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A monolithically integrated microcantilever biosensor based on partially depleted SOI CMOS technology

Microsystems & Nanoengineering 2023年第3期9卷 273-283页

作者： Yi Liu yuan tian Cong lin Jiahao Miao Xiaomei yu School of Integrated Circuits Peking UniversityNational Key Laboratory of Science and Technology on Micro/Nano FabricationBeijing 100871China

This paper presents a monolithically integrated aptasensor composed of a piezoresistive microcantilever array and an on-chip signal processing *** microcantilevers,each of them embedded with a piezoresistor,form three sensors in a Wheatstone bridge *** on-chip signal processing circuit consists of a multiplexer,a chopper instrumentation amplifier,a low-pass filter,a sigma-delta analog-to-digital converter,and a serial peripheral *** the microcantilever array and the on-chip signal processing circuit were fabricated on the single-crystalline silicon device layer of a silicon-on-insulator(SOI)wafer with partially depleted(PD)CMOS technology followed by three micromachining *** integrated microcantilever sensor makes full use of the high gauge factor of single-crystalline silicon to achieve low parasitic,latch-up,and leakage current in the PD-SOI CMOS.A measured deflection sensitivity of 0.98×10^(−6) nm^(−1) and an output voltage fluctuation of less than 1μV were obtained for the integrated microcantilever.A maximum gain of 134.97 and an input offset current of only 0.623 nA were acquired for the on-chip signal processing *** functionalizing the measurement microcantilevers with a biotin-avidin system method,human IgG,abrin,and staphylococcus enterotoxin B(SEB)were detected at a limit of detection(LOD)of 48 pg/***,multichannel detection of the three integrated microcantilever aptasensors was also verified by detecting *** these experimental results indicate that the design and process of monolithically integrated microcantilevers can meet the requirements of high-sensitivity detection of biomolecules.

关键词： SOI amplifier cantilever

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八角精油对三黄鸡生长免疫性能及肠道菌群的作用

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广西农学报 2020年第4期35卷 26-30,34页

作者：欧莹熊晓妍覃振斌虞霖田广西农业职业技术学院广西南宁530007

【目的】探究水提和乙醇提八角精油对三黄鸡生长性能、免疫性能及肠道菌群的影响。【方法】选用21日龄广西三黄鸡150只,随机分为5组,每组3个重复(10只/重复)。在不同试验组鸡的饲料中分别添加八角精油(乙醇提)0.4g/kg、八角精油(水提)0.... 详细信息

【目的】探究水提和乙醇提八角精油对三黄鸡生长性能、免疫性能及肠道菌群的影响。【方法】选用21日龄广西三黄鸡150只,随机分为5组,每组3个重复(10只/重复)。在不同试验组鸡的饲料中分别添加八角精油(乙醇提)0.4g/kg、八角精油(水提)0.4g/kg、八角精油(水提)0.8g/kg、抗生素0.06g/kg及空白对照组等5个试验组。【结果】经过21d的试验发现,八角精油0.8g/kg水提组ADFI和F/G较空白对照组降低了9.3%、10.5%(P<0.05);八角精油水提0.4g/kg和0.8g/kg组血清免疫球蛋白A比空白对照组分别提高了36.4%、44.9%(P<0.05);八角精油水提0.4g/kg和0.8g/kg组大肠杆菌菌数,比空白对照组分别降低49.88%和53.23%。【结论】水提八角精油对三黄鸡具有提高生产性能、提升部分血清免疫球蛋白水平,增强免疫性能,优化肠道菌群的作用。

关键词：八角精油三黄鸡生长性能免疫性能肠道菌群

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[Evaluation of the toxicity of perfluorooctanoic acid toward human colorectal cancer cells using multi-dimensional approaches]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 96-103页

作者： Rui-Jia Zhang Ying-Shi lin Lan-Yin Tu Zi-Tong Chen Wei-Wei Zhang tian-Gang Luan Bao-Wei Chen School of Life and Health Sciences Hainan University Haikou 570228 China. School of Life Sciences Sun Yat-sen University Guangzhou 510275 China. School of Artificial Intelligence Sun Yat-sen University Zhuhai 519082 China. Guangzhou Center for Disease Control and Prevention Guangzhou 510440 China. School of Marine Sciences Sun Yat-sen University Zhuhai 519082 China.

While human exposure to perfluorooctanoic acid (PFOA) can lead to ulcerative colitis, the molecular mechanisms responsible for PFOA-induced intestinal toxicity are unclear. Herein, we examined the toxicity of PFOA toward human colorectal cancer cells (HCT116) from three dimensions: the cytotoxic phenotype, cell respiration, and transcription levels of metabolism-related genes. Formazan was used to assess how PFOA exposure affects HCT116-cell relative viability, after which the mitochondrial respiratory activities of these cells were determined by analyzing extracellular flux. The quantitative real-time polymerase chain reaction (qPCR) method was used to detect metabolism-related gene expression levels. The cytotoxicity assay revealed that the HCT116 showed significantly inhibited relative activities compared to those of the control when exposed to 300 μmol/L PFOA for 48 h (<0.01), with most cells retained at the G0/G1 stage. In contrast, the mitochondrial respiratory activities of the HCT116 were promoted by concentrations of PFOA as low as 50 μmol/L. Two genes related to cellular metabolism (dipeptidase 1 () and sphingosine kinase 1 ()) were found to be related to the PFOA-promoted formation of ulcerative colitis using our self-developed Metabolic Gene and Pathway Query software and Comparative Toxicogenomics Database (CTD). The qPCR studies revealed that and expression levels were enhanced by 8-10 times in HCT116 exposed to 300 μmol/L PFOA relative to the control, whereas this trend was not observed for HCT116 exposed to 50 μmol/L PFOA. Collectively, these results suggest that the respiratory activity of cellular mitochondria may serve as an index for determining the interference effects associated with PFOA and that metabolic pathways mediated by and may be involved in the development of PFOA-induced ulcerative colitis. Future studies should investigate the relationships between changes in metabolism-related genes ( and ) and the mitochondrial respiratory act

关键词： Metabolic Gene and Pathway Query software human colorectal enterotoxicity metabolic disorder perfluorooctanoic acid (PFOA)

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PLG-g-mPEG Mediated Multifunctional Nanoparticles for Photoacoustic Imaging Guided Combined Chemo/Photothermal Antitumor Therapy

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Chinese Journal of Polymer Science 2023年第4期41卷 538-546,I0007页

作者： Chong Liu ling Li Zhao-Pei Guo lin lin Yan-Hui Li Hua-yu tian School of Materials Science and Engineering Changchun University of Science and TechnologyChangchun130022China Key Laboratory of Polymer Ecomaterials Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun130022China College of Chemistry and Chemical Engineering Xiamen UniversityXiamen361005China School of Materials Science and Engineering Xiamen University of TechnologyXiamen361024China

Under laser irradiation,photothermal therapy(PTT)effectively ablates tumors above 50℃.However,hyperthermia can cause additional damage due to the inevitable heat spread to surrounding healthy ***,nanoparticles named as GI@P NPs were designed for enhanced PTT with heat shock protein 90(HSP90)inhibition at temperatures below 50℃to achieve optimal cancer therapy and avoid surrounding ***@P NPs were done by co-loading Garcinia cambogia acid(GA)and photosensitizer IR783 in polymer PLG-g-mPEG to form a nanomedicine,where IR783 with excellent photoacoustic(PA)signal acted as an excellent photothermal therapeutic agent that converted the laser energy into heat to kill tumor cells,GA was used as antitumor drug for chemotherapy and an inhibitor of HSP90 to overcome the heat resistance of tumors for efficient cryo-photothermal therapy,and PLG-g-mPEG can encapsulate IR783 and GA to increase biocompatibility and accumulate effectively in the *** GI@P NPs were injected into the mice,we could observe that the PA signals gradually increased in the tumor region and showed the strongest PA signals at 12 *** laser irradiation,the tumor temperature of the mice could raise to about 43.5℃,and the tumor was significantly inhibited after long-term monitoring by PA *** a result,gentle PTT produced by GI@P NPs exhibited good antitumor effects at relatively low temperature and minimized nonspecific thermal damage to normal *** GI@P NPs as nanomedicine enriched our understanding of various applications of polymeric carriers,especially in the biomedical field.

关键词： Mild-temperature photothermal therapy Photoacoustic imaging Polymer carrier Combination therapy Co-loading

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促卵泡素对雏鸡原始卵泡形成的影响

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南方农业学报 2024年第9期55卷 2772-2782页

作者：崔毓菲杨灿灿凌卫东刘思琪彭睿梁炅明虞霖田陆阳清广西大学动物科学技术学院广西南宁530004

【目的】探究促卵泡素(FSH)对雏鸡原始卵泡形成的影响,为开展雏鸡卵巢的早期发育及提高蛋鸡产蛋性能的研究提供理论依据。【方法】将40只刚出壳雏鸡随机分为试验组(FSH)和对照组(CK),分别肌肉注射20 IU/mL FSH和生理盐水。试验开始后0和... 详细信息

【目的】探究促卵泡素(FSH)对雏鸡原始卵泡形成的影响,为开展雏鸡卵巢的早期发育及提高蛋鸡产蛋性能的研究提供理论依据。【方法】将40只刚出壳雏鸡随机分为试验组(FSH)和对照组(CK),分别肌肉注射20 IU/mL FSH和生理盐水。试验开始后0和5.5 d称取雏鸡体重,5.5 d采集雏鸡卵巢组织进行石蜡切片、HE染色和转录组测序(RNA-Seq)分析。通过生物信息学分析筛选差异表达基因(DEGs),并进行GO功能注释和KEGG信号通路富集分析。将DEGs上传STRING数据库构建蛋白互作(PPI)网络。最后通过实时荧光定量PCR检测基因相对表达量。【结果】与CK组相比,FSH组雏鸡体重无显著变化(P>0.05,下同),卵巢体积明显增大。HE染色结果表明,FSH组卵巢皮质较厚,卵巢组织更致密,原始卵泡数量增多且体积增大,能明显看到原始卵泡结构。2组雏鸡卵巢中共存在617个DEGs,FSH组有107个,CK组有510个。GO功能注释和KEGG信号通路富集分析结果表明,DEGs注释到的GO功能条目可分为生物学过程、细胞组分和分子功能三大类,主要富集到PI3K-AKT、Wnt/β-catenin、MEK/ERK和MEK1/2/ERK1/2信号通路。实时荧光定量PCR结果显示,与CK组相比,FSH组DAZL和PIWIL1基因相对表达量极显著降低(P<0.01,下同),SMAD2、ZP1和CYP17A1基因相对表达量显著(P<0.05)或极显著升高,FSHR基因相对表达量无显著变化。【结论】FSH可能是通过影响颗粒细胞增殖、类固醇生成和血管生成等多条卵泡发育相关信号通路来促进雏鸡原始卵泡形成,其对CYP17A1、SMAD2和ZP1等基因的调控可能是其促进原始卵泡生成的主要分子机制之一。

关键词：鸡促卵泡素卵巢原始卵泡 RNA-Seq

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Metastable seed-crystals: epitaxial growth of branched-array organic heterostructure nanowires

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Science China Chemistry 2025年

作者： Jin Feng Zhen-yu Geng Ting-Yao Song Ying-Xin Ma Chuan-Zeng Wang Wei Zhang Hong-Tao lin Jin Zhou Shu-Ping Zhuo Shu-Hai Chen Xue-Dong Wang School of Chemistry and Chemical Engineering Shandong University of Technology Institute of Functional Nano & Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials & DevicesSoochow University College of Chemistry and Chemical Engineering Hunan Normal University

Tunable light–matter interactions are exhibited by organic low-dimensional crystals, making these crystals a promising platform for organic photonics. However, the precise synthesis of organic low-dimensional crystals remains challenging due to the stochastic nature of molecular nucleation processes. Herein, the directed nucleation process is driven by the introduction of metastable seed-crystals as the trunk, which ultimately leads to branched-array organic heterostructures. The successful formation of organic heterostructures with high-density branched arrays is attributed to the highest attachment energy (Eatt（023）=-104.25 kcal mol-1) of the exposed （023） crystal plane during the seed-crystal growth route. Significantly, these as-prepared heterostructures inherently have an ultralow lattice mismatch ratio η of 0.7%between trunk and branch, which contributes to the multi-channel photon transportation. Therefore, this work provides valuable insights into a versatile synthetic strategy for accessing low-dimensional heterostructures for integrated optoelectronics.

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Double [5]carbohelicene: facile synthesis, chiroptical properties,isomerization study, and lasing application

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Science China Chemistry 2025年第1期 233-240页

作者： Peipei Liu Yantong Li Meng-Xiang Wu Hao Kang Xiao-Li Zhao lin Xu linlin Liu Xiaodong Li Junfeng Fang Zhiwei Fang Ya Cheng Hai-Bo Yang Huakang yu Xueliang Shi State Key Laboratory of Petroleum Molecular & Process Engineering Shanghai Key Laboratory of Green Chemistry and Chemical ProcessesSchool of Chemistry and Molecular Engineering East China Normal University School of Physics and Optoelectronics South China University of Technology Institute of Polymer Optoelectronic Materials & Devices State Key Laboratory of Luminescent Materials & Devices South China University of Technology School of Physics and Electronic Science Engineering Research Center of Nanophotonics & Advanced Instrument Ministry of EducationEast China Normal University State Key Laboratory of Precision Spectroscopy School of Physics and Electronic Science East China Normal University

Double carbohelicenes have attracted considerable attention due to their aesthetic structures, distinct π-conjugation extension, inherent chirality, and intriguing optical and electronic properties. Herein, the concise de novo synthesis of a new double[5]carbohelicene 1 together with its chiroptical properties, isomerization process and lasing application is presented. 1 was synthesized by a simple protocol involving the formation of a 6,6′-bipentacene as the key intermediate. It is worth noting that1 existed as two diastereomers 1-PP/MM and 1-PM, which were successfully isolated and unambiguously confirmed by Xray crystallography. The optical resolution of the racemic 1-PP/MM was successfully achieved by chiral-phase highperformance liquid chromatography（HPLC）, and 1-PP and 1-MM were characterized by circular dichroism. Interestingly, it was found that 1-PP/MM could be completely isomerized to 1-PM upon heating, and the detailed thermodynamics and kinetics of the isomerization process were systematically investigated. 1-PM exhibited deep-red luminescence with emission maximum at 723 nm and fluorescence quantum yield as high as 32.95%. Consequently, we can showcase the lasing application of 1-PM by doping with epoxy resin to assemble a bottle microlaser spontaneously, which the lasing wavelength is 796.6 nm at a threshold of 0.8 m J cm-2. We believe that our studies, including the facile synthesis methodology, detailed isomerization studies, and lasing application, will shed some light on the design and synthesis of novel carbohelical systems with more functions.

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Lumped parameter model for resonant frequency estimation of a thermoacoustic engine with gas-liquid coupling oscillation

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第3期12卷 232-237页

作者： Ke TANG tian LEI Xiao-gang lin Tao JIN yu ZHANG Institute of Refrigeration and Cryogenics Zhejiang University Hangzhou 310027 China

Gas-liquid coupling oscillation is a novel approach to reducing the resonant frequency and to elevating the pressure amplitude of a thermoacoustic *** a thermoacoustic engine is used to drive low-frequency pulse tube refrigerators,the frequency matching between the thermoacoustic engine and the refrigerator plays an important *** on an acoustic-electric analogy,a lumped parameter model is proposed to estimate the resonant frequency of a standing-wave thermoacoustic engine with gas-liquid coupling ***,a simplified lumped parameter model is also developed to reduce the computation *** resonant frequency dependence on the mean pressure,the gas space volume,and the water column length is computed and *** impact of different working gases on the resonant frequency is also *** effectiveness of the models is validated by comparing the computed results with the experimental data of the gas-liquid coupling oscillation *** increase in the mean working pressure can lead to a rise in the resonant frequency,and a lower resonant frequency can be achieved by elongating the liquid *** comparison with nitrogen and argon,carbon dioxide can realize a lower frequency due to a smaller specific heat ratio.

关键词： Thermoacoustic engine Gas-liquid coupling oscillation Acoustic-electric analogy Resonant frequency

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Skeletal muscle-specific Bambi deletion induces hypertrophy and oxidative switching coupling with adipocyte thermogenesis against metabolic disorders

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Science China Life Sciences 2025年

作者： Xiangping Yao Xudong Mai Ye tian Yifan Liu Guanghui Jin Ze Li Shujie Chen Xiaoshuang Dai Liujing Huang Zijing Fan Guihua Pan Xiaohan Pan Xiangmin Li Michael C yu Jia Sun Jingxing Ou Hong Chen Liwei Xie College of Life and Health Sciences Guangdong Industry Polytechnic Department of Endocrinology and Metabolism Zhujiang Hospital Southern Medical University State Key Laboratory of Applied Microbiology Southern China Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application Guangdong Open Laboratory of Applied MicrobiologyInstitute of Microbiology Guangdong Academy of Sciences Institute of Aging Research Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics School of Medical Technology Guangdong Medical University Department of Hepatic Surgery and Liver transplantation Center of the Third Affiliated Hospital Organ Transplantation Institute Sun Yat-sen University Organ Transplantation Research Center of Guangdong ProvinceGuangdong province engineering laboratory for transplantation medicine Guangdong Provincial Key Laboratory of Liver Disease Research School of Public Health Xinxiang Medical University BGI Institute of Applied Agriculture BGI-Shenzhen Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic University Department of Biological Sciences State University of New York at Buffalo Department of Internal Medicine Shunde Women and Children's Hospital (Maternity and Child Healthcare Hospital of Shunde Foshan) Guangdong Medical University

Skeletal muscle plays a significant role in both local and systemic energy metabolism. The current investigation aims to explore the role of the Bambi gene in skeletal muscle, focusing on its implications for muscle hypertrophy and systemic metabolism. We hypothesize that skeletal muscle-specific deletion of Bambi induces muscle hypertrophy, improves metabolic performance, and activates thermogenic adipocytes via the reprogramming of progenitor of i WAT, offering potential therapeutic strategies for metabolic syndromes. Leveraging the Chromatin immunoprecipitation(ChIP)-seq and bioinformatics analysis, Bambi gene is shown to be a direct target of HIF2α, which is further confirmed by ChIP-qPCR and promoter luciferase assay. Skeletal muscle-specific Bambi deletion led to significant muscle hypertrophy and improved metabolic parameters, even under high-fat diet conditions. This deletion induced metabolic reprogramming of stromal vascular fractions(SVFs) into thermogenic adipocytes, contributing to systemic metabolic improvements, potentially through the secretory ***, mice with skeletal muscle-specific Bambi deletion demonstrate resistance to high-fat diet-induced metabolic disorders, highlighting a potential therapeutic pathway for metabolic syndrome management. Thus, skeletal muscle-specific deletion of Bambi triggers muscle growth, enhances metabolic performance, and activates thermogenic adipocytes. These findings suggest Bambi as a novel therapeutic target for metabolic syndromes, providing new insights into the interaction between muscle hypertrophy and systemic metabolic improvement. The study underscores the potential of manipulating muscle physiology to regulate whole-body metabolism, offering a novel perspective on treating metabolic disorders.

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The interaction between dietary marine components and intestinal flora

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Marine Life Science & Technology 2020年第2期2卷 161-171页

作者： lin Li Chang-Hu Xue tian-tian Zhang yu-Ming Wang College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The intestine is the natural habitat for a large and dynamic bacterial community,which perform vital metabolic and immune functions that significantly effect the nutritional and health status of the *** major physiological functions of intestinal flora include metabolic activities that lead to important trophic effects on immune structure and function as well as protection of the colonized host against invasion by transient *** intestinal flora is both a target for nutritional intervention and a factor influencing the biological activity of food compounds acquired *** environments,as a source of diverse organic compounds,can supply us with a number of beneficial and health-promoting *** review focuses on the physiological functions of intestinal flora and reciprocal interactions between intestinal flora and dietary marine components including polysaccharides,lipids,protein,taurine,carotenoids,minerals,and *** review can serve as a valuable reference regarding the consumption of seafood and marine nutraceuticals.

关键词： Intestinal flora Physiological function Dietary marine components Interaction

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