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Neural Regeneration Research 2017年第11期12卷 1832-1842页

作者： Jia-nan Guo lin-yu tian Wen-yu Liu lie Mu Dong Zhou Department of Neurology West China HospitalSichuan UniversityChengduSichuan ProvinceChina

Survivors of sepsis may suffer chronic cognitive impairment as a long-term sequela. However, the precise mechanisms of cognitive dys- function after sepsis are not well understood. We employed the cecal ligation-and-puncture-induced septic mouse model. We observed elevated phosphorylation of Akt, mammalian target of rapamycin （roTOR） and p70S6K on days 14 and 60, progressive neuronal loss in the cornu ammonis 1 region, and abnormal neuronal morphology in the hippocampus in the sepsis mouse model, These findings indicate that changes in neuronal morphology and number in the hippocampus after sepsis were associated with strong activation of the Akt/mTOR sig- naling pathway, and may reflect a ＂self-rescuing＂ feedback response to neuronal loss after sepsis.

关键词： nerve regeneration sepsis cognitive impairment Akt mTOR p 7OS6K hippocampus neurons phosphorylation neural hypertrophy neural regeneration

Homeobox protein A1-like and DNA methylation regulate embryo-specific Zinc finger protein 615 gene expression and embryonic development in the silkworm Bombyx mori

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Insect Science 2023年第4期30卷 1063-1080页

作者： yu-lin tian Tong-yu Fu Qi-En Zhong Yi-Guang lin Si-Chun Zheng Guan-Feng Xu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Applied ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

DNA methylation and transcription factors play roles in gene expression and animal *** insects,DNA methylation modifies gene bodies,but how DNA methylation and transcription factors regulate gene expression is *** this study,we investigated the mechanism that regulates the expression of Bombyx mori Zinc finger pro-tein 615(ZnF 615),which is a downstream gene of DNA methyltransferase 1(Dnmt1),and its effects on the regulation of embryonic *** progressively truncating the ZnF 615 promoter,it was found that the-223 and-190 nt region,which contains homeobox(Hox)protein cis-regulatory elements(CREs),had the greatest impact on the transcription of ZnF *** interference(RNAi)-mediated knockdown and overexpres-sion of Hox family genes showed that Hox A1-like can enhance the messenger RNA level of ZnF *** studies showed that Hox A1-like regulates ZnF 615 expression by directly binding to the-223 and-190 nt region of its *** RNAi-mediated knockdown or overexpression of Hox A1-like and Dnmtl significantly inhibited or enhanced the regulatory effect of either gene alone on ZnF 615 expression,suggesting that both DNA methylation of gene bodies and binding of transcription factors to pro-moters are essential for gene ***-mediated knockdown of Hox A1-like and Dnmtl showed that the embryonic development was retarded and the hatching rate was *** together,these data suggest that Hox A1-like and DNA methylation en-hance the expression of ZnF 615,thereby affecting the development of *** embryos.

关键词： Bombyx mori DNA methylation embryo gene expression Hox Al-like ovary

Methyl-CpG binding domain(MBD)2/3 specifically recognizes and binds to the genomic mCpG site with a β-sheet in the MBD to affect embryonic development in Bombyx mori

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Insect Science 2023年第6期30卷 1607-1621页

作者： Tong-yu Fu Shuang-Shun Ji yu-lin tian Yi-Guang lin yu-Mei Chen Qi-En Zhong Si-Chun Zheng Guan-Feng Xu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina

Methyl-CpG(mCpG)binding domain(MBD)proteins especially bind with methylated DNA,and are involved in many important biological processes;however,the binding mechanism between insect MBD2/3 and mCpG remains *** this study,we identified 2 isoforms of the MBD2/3 gene in Bombyx mori,MBD2/3-S and MBD2/*** analysis of MBD2/3-L,MBD2/3-S,and 7 mutant MBD2/3-L proteins deficient inβ1−β6 orα1 in the MBD showed thatβ2−β3-turns in theβ-sheet of the MBD are necessary for the formation of the MBD2/3–mCpG complex;furthermore,other secondary structures,namely,β4−β6 and anα-helix,play a role in stabilizing theβ-sheet structure to ensure that the MBD is able to bind *** addition,sequence alignment and binding analyses of different insect MBD2/3s indicated that insect MBD2/3s have an intact and conserved MBD that binds to the mCpG of target ***,MBD2/3 RNA interference results showed that MBD2/3-L plays a role in regulating *** embryonic development,similar to that of DNA methylation;however,MBD2/3-S withoutβ4−β6 andα-helix does not alter embryonic *** results suggest that MBD2/3-L recognizes and binds to mCpG through the intactβ-sheet structure in its MBD,thus ensuring silkworm embryonic development.

关键词： embryonic development insects MBD2/3 methyl-CpG binding domain(MBD) β-sheet

An efficient method for preparing the sphingosine-1-phosphate receptor agonist, KRP203-phosphate

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Chinese Chemical Letters 2013年第9期24卷 817-820页

作者： Xiao-Jian Wang Jian-Guo Qi Gang Li yu-lin tian Da-Li Yin State Key Laboratory of Bioactive Substances and Functions of Natural Medicines Beijing Key Laboratory of Active Substance Discovery and Drugability EvaluationInstitute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences

An efficient method for mono-phosphorylation of 2 （KRP203） using cbz-protection, dibenzylphosphoryl chloride and TMSI affording 2-P （KRP203-P） was developed. We applied the present method to the synthesis of KRP20... 详细信息

关键词： Mono-phosphorylation TMSI KRP203-P

An efficient synthesis of substituted 1,4-diazepines by a Pd catalyzed amination and sequential hydrogenation condensation

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Chinese Chemical Letters 2013年第8期24卷 743-746页

作者： Xiao-Jian Wang yu-lin tian Qing-Yang Zhang Jian-Guo Qi Da-Li Yin State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing Key Laboratory of Active Substance Discovery and Druggability EvaluationInstitute of Materia MedicaPeking Union Medical College and Chinese Academy of Medical Sciences

An efficient synthesis of substituted 1,4-diazepines is *** accessible intermediates have been obtained via Pd-catalyzed *** subsequent hydrogenation and intramolecular condensation sequences could be conducted successively in one pot without special *** mild and general strategy enables the synthesis of various substituted 1,4-diazepines in high yields.

关键词： 1 4-Diazepines Pd-catalyzed amination Hydrogenation Intramolecular condensation

DNA methylation-mediated expression of zinc finger protein 615 affects embryonic development in Bombyx mori

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Zoological Research 2022年第4期43卷 552-565页

作者： Guan-Feng Xu Cheng-Cheng Gong yu-lin tian Tong-yu Fu Yi-Guang lin Hao Lyu yu-ling Peng Chun-Mei Tong Qi-Li Feng Qi-Sheng Song Si-Chun Zheng Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Applied ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouGuangdong 510631China Division of Plant Science and Technology College of AgricultureFood and Natural ResourcesUniversity of MissouriColumbia MO 65211USA

Cell division and differentiation after egg fertilization are critical steps in the development of embryos from single cells to multicellular individuals and are regulated by DNA methylation via its effects on gene ***,the mechanisms by which DNA methylation regulates these processes in insects remain ***,we studied the impacts of DNA methylation on early embryonic development in Bombyx *** methylation and transcriptome analysis of early embryos showed that DNA methylation events mainly occurred in the 5'region of protein metabolism-related *** transcription factor gene zinc finger protein 615(ZnF615)was methylated by DNA methyltransferase 1(Dnmt1)to be up-regulated and bind to protein metabolism-related ***1 RNA interference(RNAi)revealed that DNA methylation mainly regulated the expression of nonmethylated nutrient metabolism-related genes through *** same sites in the ZnF615 gene were methylated in ovaries and *** of ZnF615 using CRISPR/Cas9 gene editing decreased the hatching rate and egg number to levels similar to that of Dnmt1 *** of the ZnF615 methylation rate revealed that the DNA methylation pattern in the parent ovary was maintained and doubled in the offspring ***,Dnmt1-mediated intragenic DNA methylation of the transcription factor ZnF615 enhances its expression to ensure ovarian and embryonic development.

关键词： DNA methylation Embryonic development Transcriptional regulation Epigenetic

One pot synthesis of dibenzodiazepinones via CuI catalysis in ethylene glycol

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Chinese Chemical Letters 2013年第9期24卷 825-828页

作者： Qing-Yang Zhang Xiao-Jian Wang yu-lin tian Jian-Guo Qi Chun Li Da-Li Yin State Key Laboratory of Bioactive Substance and Function of Natural Medicines Beijing Key Laboratory of Active Substance Discovery and Druggability Evaluation Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences

A one pot protocol for the synthesis of dibenzodiazepinones was developed. The substituted ethyl 2- halobenzoates are cross-coupled with o-phenylenediamine utilizing a ligand-free, Cul catalyzed system, which spontaneously undergo an intramolecular N-acylation in ethylene glycol to give the corresponding products in high yields. This synthetic protocol provides a concise and efficient access to a wide variety of dibenzodiazepinone, including biologically active molecules.

关键词： One pot Copper Cyclization Dibenzodiazepinone

Clinicopathological significance of Bcl-2 and Bax protein expression in human pancreatic cancer

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World Journal of Gastroenterology 2005年第18期11卷 2744-2747页

作者： MingDong Jian-PingZhou HaoZhang Ke-JanGuo yu-lin-tian yu-TingDong DepartmentofSurgery FirstAffiliatedHospitalChinaMedicalUniversityShenyangllO00lLiaoningProvinceChina

AIM: To assess the clinicopathological significance of the expression of the apoptosis-inhibitory Bcl-2 protein (pBcl-2) and the apoptosis-promoting Bax protein (pBax) in human invasive ductal carcinomas (IDCs) of the pancreas. METHODS: Fifty-nine surgical specimens of IDCs of the pancreas were stained immunohistochemically to detect pBcl-2 and pBax expressions whose correlation to tumor classification, staging, and prognosis was analyzed by univariate and multivariate analyses. RESULTS: The expression of pBcl-2 and pBax was detected in 21 of 59 (35.6%) and in 29 of 59 (49.2%) patients with IDCs of the pancreas, respectively. Neither pBcl-2 nor pBax alone was correlated to TIMM staging and differentiation degree of IDCs of the pancreas according to univariate analysis. By Mantel-Cox test, the median survival time after surgery for pBcl-2(+) and pBcl-2(-) groups were 14.3 and 7.3 mo, respectively (x^2=9.357, P=0.002) and that for pBax(+) and pBax(-) groups were 12.9 and 10.2 mo, respectively (x^2=0.285, P＞0.05). Contingency coefficient between pBcl-2 and pBax expression was 0.298, indicating that there was correlation between them (x^2=5.74, P＜0.05). The median survival time after surgery for pBcl-2(+)pBax(+) and pBcl-2(+)pBax(-) groups were 14.3 and 14.1 mo, respectively, and that for pBcl-2 (-)pBax(+) and pBcl-2(-)pBax(-) groups were 5.9 and 9.9 mo, respectively. There was a significant difference between pBd-2(+)pBax(+) and pBd-2(-)pBax(+) (x^2=5.06, P＜0.05), such was the case for pBcl-2(+)pBax(+) and pBd-2(-)pBax(-) (x~ 2=7.18, P＜0.01). Cox proportional hazards model for multivariate analysis was applied, indicating that pBcl-2, TNM staging, age and pBax were high risk factors of post-surgical survival time. CONCLUSION: Both pBcl-2 and pBax have high expression in IDCs of the pancreas, indicating that co-expression of pBcl-2 and pBax is a good indicator of favorable prognosis in IDCs of the pancreas.

关键词： Pancreatic cancer pBcl-2 pBax Immunohistochemistry

Optimization of multi-reflection time-of-flight mass analyzer operating in in-trap-lift mode

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Radiation Detection Technology and Methods 2018年第1期2卷 1-6页

作者： Wen-Xue Huang yu-lin tian Yong-Sheng Wang Jun-Ying Wang Xiao-Hong Zhou Institute of Modern Physics Chinese Academy of SciencesLanzhou730000China School of Physical Sciences University of Chinese Academy of SciencesBeijing100049China School of Nuclear Science and Technology Lanzhou UniversityLanzhou730000China

Purpose We are building an MRTOF-MS(multi-reflection time-of-flight mass spectrometer)for isobaric separation for the Lanzhou Penning *** potentials applied on the electrodes of our MRTOF mass analyzer operating in in-trap-lift mode have to be optimized to achieve a very high mass resolving *** Our method to design and optimize an MRTOF mass analyzer has been updated to introduce constraints on the potentials,and this method now can be used to optimize the parameters of MRTOF-MS both operating in mirror-switching mode and in in-trap-lift *** using this method,the optimal potential parameters of the electrodes have been obtained for our MRTOF mass analyzer operating in the in-trap-lift *** and conclusion With a beam size of 2.8 mm diameter and an initial average ion kinetic energy of 1500 eV,the maximal mass resolving power has been achieved to be 3.2×10^(4) with a total TOF of 7.0 ms for an ion species of ^(40)Ar^(1+).It can reach up to 5.6×10^(4) for a beam size of 0.3 mm *** simulation shows that the inaccuracy of the potentials applied on the outermost mirror electrodes M1–M2 must be less than 50 ppm or preferably 20 ppm.

关键词： Time-of-flight mass spectrometer Multiple reflection Mass measurement Isobaric separation Exotic nuclei

Surgical management of 143 patients with adult primary retroperitoneal tumor

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World Journal of Gastroenterology 2007年第18期13卷 2619-2621页

作者： yuan-Hong Xu Ke-Jian Guo R.en-Xuan Guo Chun-lin Ge yu-lin tian San-Guang He Department of General Surgery The First Affiliated Hospital of China Medical UniversityShenyang 110001 Liaoning Province China

AIM： To analyze the surgical management of adult primary retroperitoneal tumors （APRT） and the factors influencing the outcome after operation. METHODS： Data of 143 cases of APRT from 1990 to 2003 in the First Affiliated Hospital of China Hedical University were evaluated retrospectively. RESULTS： A total of 143 cases of APRT were treated surgically. Among them, 122 （85.3%） underwent complete resection, 16 （11.2%） incomplete resection, and 3 （3%） surgical biopsies. Twenty-nine （20.2%） underwent tumor resection plus multiple organ resections. Ninety-five malignant cases were followed up for 1 mo to 5 years. The 1-year, 3-year, and 5-year survival rates of the patients subject to complete resection was 94.9%, 76.6% and 34.3% and that of patients with incomplete resection was 80.4%, 6.7%, and 0%, respectively （P 〈 0.001）. The Cox multi-various regression analysis showed the completeness of tumor, sex and histological type were associated closely with local recurrence. CONCLUSION： Sufficient preoperative preparation and complete tumor resection play important roles in redudng recurrence and improving survival.

关键词： Retroperitoneal neoplasms Surgical procedure OperaUve Recurrence