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Study on low-temperature lipase of psychrophilic bacterium 2.5-1.-1.isolated from deep sea of Southern {2.

Acta oceanologica Sinica 2003年第4期22卷 643-649页

作者： lin xuezheng1,{1.,{2.,huang xiaohang1 1. first institute of oceanography,state oceanic administration,qingdao 266061,china 2. fishery college, ocean university of china,qingdao 266003,china state {1. Adm Inst Oceanog 1 Qingdao 266061 Peoples R China ocean Univ {1. Fishery Coll Qingdao 266003 Peoples R China

A strain of psychrophilic bacterium, 2.5-1.-1. which produces low-temperature lipase, is isolated from the deep sea of Prydz Bay in Southern {2.. The highest lipase secretion of this strain is observed at 5 degreesC and this temperature is also for optimal growth. Tween 80 and olive oil enhance secretion of lipase. The optimal temperature and pH for lipase activity are 35 degreesC and 7.5 degreesC respectively. At 0degreesC, the lipase still has 37% relative enzyme activity. The lipase shows high thermolability, more than 50% activity lost after incubation at 60 degreesC for 1. min. EDTA has no effect on lipase activity, indicating the lipase activity is independent of divalent cation. In contrast, the lipase activity is inhibited drastically by Cu2. and Zn2..

关键词： Southern ocean deep sea psychrophilic bacteria low-temperature lipase

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东海北部和黄海南部鳀鱼卵和仔稚幼鱼数量、分布及其与环境条件的关系

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水产学报 2002年第4期26卷 321-330页

作者：万瑞景黄大吉张经中国水产科学研究院黄海水产研究所国家海洋局第二海洋研究所华东师范大学河口海岸国家重点实验室山东青岛 2.6071 浙江杭州 31.01. 上海 2.0062

1.99年夏季鳀鱼卵和仔稚幼鱼主要分布于黄海南部,1.2.00′～1.3°45′E、34°00′～35°1.′N与1.2.1.′E以西海域分别为鳀鱼卵与鳀鱼仔稚幼鱼的密集分布中心,其表层水温和盐度分别为2..5～2..9℃和2..1.～31.81.2..4～2..0℃和31.31...

1.99年夏季鳀鱼卵和仔稚幼鱼主要分布于黄海南部,1.2.00′～1.3°45′E、34°00′～35°1.′N与1.2.1.′E以西海域分别为鳀鱼卵与鳀鱼仔稚幼鱼的密集分布中心,其表层水温和盐度分别为2..5～2..9℃和2..1.～31.81.2..4～2..0℃和31.31.32.05。鳀鱼卵和仔稚幼鱼分布的最适表层水温分别为2..5～2..4℃和2..9～2..0℃;全长为2.mm以上的鳀鱼稚鱼已初步形成广盐性分布的习性,鳀鱼卵和仔稚幼鱼的分布对盐度的要求并不非常严格,水温是影响鱼卵和仔稚幼鱼分布的主要原因之一。鳀鱼卵和仔稚幼鱼的分布与叶绿素a的分布也有密切的关系,鳀鱼卵和仔稚幼鱼的密集分布中心正是叶绿素a的高值区。随着季节的变化,黄海南部鳀鱼产卵场由青岛外海逐渐向深水区转移。黄海南部鳀鱼活卵所占的比例为45.76％,胚胎发育状况良好。

关键词：鳀鱼卵仔稚幼鱼胚胎发育环境条件东海北部黄海南部

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Identification of SNARE proteins in fish—Tilapia Oreochromis niloticus

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Acta oceanologica Sinica 2007年第1期26卷 159-163,164页

作者： huang {1. LAM Patrick P L {2. xuezheng LIU Chen{2. bian ji GAISANO Herbert Key Laboratory of Marine Bioactive Substances First Institute of Oceanography State Oceanic Administration Qingdao 266061 China Department of Physiology and Medicine University of Toronto Toronto M5S 1A8 Canada

SNARE proteins are a group of membrane-associated proteins involved in exocytosis, secretion and membrane trafficking events in eukaryotic cells. Research on SNARE protein biology has become a more attractive field in recent years, which is applied to marine biology specifically to the fish Tilapia （Oreochromis niloticus）. Plasma membrane fractions of different tissues of Tilapia, including brain, liver-pancreas, intestine, skin and muscle, were extracted, and immuno-decorated with isoform-specific antibodies to the SNARE families and associated proteins. The presence of Syntaxins - 1.A, 2.and 3, SNAP - 2. and SNAP - 2., VAMP - 2. Munc - 1. - 1.and Munc - 1. in the brain was identified, which were differentially distributed in the other organ tissues of the fish Tilapia. The distinct distribution of SNARE and associated proteins will serve as the basis for further investigation into their special secretory function in these tissues of the fish.

关键词： SNARE proteins exocytosis secretion fish

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Screening and Characterization of the Alkaline Protease Isolated from PLI-1. a Strain of Brevibacillus sp. Collected from Indonesia's Hot Springs

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Journal of ocean university of china 2012年第2期11卷 213-218页

作者： WANG Shuai lin {1. {2. xiaohang ZHENG Li Dewi Seswita Zilda The Key Laboratory of Marine Bioactive Substances The First Institute of OceanographySOAQingdao 266061P R.China Research Center for Marine and fishery Product Processing and Biotechnology JakartaIndonesia

A total of 69 strains of thermophilic bacteria were isolated from water, soil and sediment samples from three Indonesia's hot spring areas (Pantai cermin, Kalianda and Banyu wedang) by using Minimal Synthetic Medium (MSM). The extreme thermophile Brevibacillus sp. PLI-1.was found to produce extracellular thermophilic alkaline protease with optimal activity at 70℃ and pH 8.0-9.0. The molecular weight of the protease was estimated to be around 56 kD by SDS-PAGE. The maximum activity of the protease was 2..54 U mL-1. The protease activity did not decrease after 30 min and still retained more than 70% of relative activity after 60 min at 70℃ and pH 8.0. The ion Mg2. was found to promote protease activity at both low and high concentrations, whereas Cu2. and Zn2. could almost completely inhibit the activity. Divalent cation chelator EDTA inhibited the enzyme activity by 55.06% ± 0.2.%, while the inhibition caused by PMSF, Leupeptin, Pepstain A and Benzamidine were 66.78% ± 3.2.%, 52.37% ± 0.2.%, 62.47% ± 2.96% and 50.99% ± 0.2.%, respectively. Based on these observations, the enzyme activity was conspicuously sensitive to the serine and cysteine protease inhibitors. All these results indicated that the protease isolated from the strain PLI-1.was a thermophilic protease and had a high-temperature stability and a pH stability.

关键词： hot spring thermophilic bacterium thermophilic protease identification

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