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Food Science and Human Wellness 2024年第3期13卷 1135-1151页

作者： Enning zhou Qiangqiang Li Dan Zhu Gang Chen liming Wu Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing 100093China Department of Food Science University of OtagoDunedin 9016New Zealand School of Food and Health Beijing Technology and Business UniversityBeijing 100048China

Food allergens are mainly naturally-occurring proteins with immunoglobulin E(IgE)-binding epitopes.Understanding the structural and immunogenic characteristics of allergenic proteins is essential in assessing whether and how food processing techniques reduce allergenicity.We here discuss the impacts of food processing technologies on the modification of physicochemical,structural,and immunogenic properties of allergenic proteins.Detection techniques for characterizing changes in these properties of food allergens are summarized.Food processing helps to reduce allergenicity by aggregating or denaturing proteins,which masks,modifies,or destroys antigenic epitopes,whereas,it cannot eliminate allergenicity completely,and sometimes even improves allergenicity by exposing new epitopes.Moreover,most food processing techniques have been tested on purified food allergens rather than food products due to potential interference of other food components.We provide guidance for further development of processing operations that can decrease the allergenicity of allergenic food proteins without negatively impacting the nutritional profile.

关键词： Food allergens Protein structural characterization Immunogenicity evaluation Food processing modification

Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice

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Food Science and Human Wellness 2024年第1期13卷 349-359页

作者： Shuting Yin Qiangqiang Li Yuxiao Tao Enning zhou Kun Wang Wanwen Chen Xiangxin Li liming Wu Institute of Apicultural Research Chinese Academy of Agricultural SciencesBeijing 100093China Department of Animal Science McGill UniversityQuebec H9X 3V9Canada State Key Laboratory of Food Science and Technology School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.

关键词： Alleviatory effects Lactic acid bacteria fermentation Bee pollen allergen Gut microbiota Host metabolism

Computational fluid dynamics simulation and experimental study on mixing performance of a three-dimensional circular cyclone-type microreactor

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Chinese Chemical Letters 2024年第4期35卷 382-388页

作者： Shiyu Hou Maolin Sun liming Cao Chaoming Liang Jiaxin Yang Xinggui zhou Jinxing Ye Ruihua Cheng School of Chemical Engineering East China University of Science and TechnologyShanghai 200237China School of Biomedical and Pharmaceutical Sciences Guangdong University of TechnologyGuangzhou 510006China School of Pharmacy East China University of Science and TechnologyShanghai 200237China

Microchannels enable the fast and efficient mixing of multiphase fluids.In this study, a millimeter-scale three-dimensional(3D) circular cyclone-type microreactor was designed for the mixing.The flow characteristics and mixing intensity were simulated by computational fluid dynamics simulations at a flow rate range of 12–96 m L/min using a water/ethyl acetate system.In the 3D variable-diameter structure,the microreactor induced paired opposite vortices and abruptly changed the local pressure to achieve a stable turbulent effect within the theoretical range of laminar flow.Tracer injection simulations indicated that sufficient mixing units successfully promote fluid dispersion.Diazo-coupling experiments showed a segregation index of X_S= 0.00,039 within a residence time of 9 s.Extraction experiments on the nbutanol/succinic acid/water system showed that the 3D circular cyclone-type microreactor achieved 100%extraction efficiency(E) in 4.25 s, and the overall volume mass transfer coefficient(K_La) reached 0.05–1.5s^(-1) in 12–96 m L/min.The isolated yield of the phase transfer alkylation and oxidation reactions in the 3D circular cyclone-type microreactor achieved 99% within 36 s, which was superior to the coil microreactor and batch reactor.

关键词： Microfluidics Passive microreactor CFD simulation Extraction Liquid-liquid biphase reaction

Effect of yttrium on catalytic performance of Y-doped TiO_(2) catalysts for propane dehydrogenation

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Journal of Rare Earths 2024年第5期42卷 962-971,I0005页

作者： Liyu Hou liming Xia Ruipeng zhou Jianmei Li Renjie Li Zhen Zhao Guimei Yuan Aijun Duan State Key Laboratory of Heavy Oil Processing China University of PetroleumBeijing102249China Institute of Catalysis for Energy and Environment College of Chemistry and Chemical EngineeringShenyang Normal UniversityShenyang110034China

Defective bulk catalysts based on TiO_(2) have superior catalytic performance for propane dehydrogenation(PDH).The oxygen vacancy concentration and the number of active sites on the catalyst surface can be effectively tuned by doping metal in TiO_(2).Herein,yttrium(Y)-doped titanium dioxide(nY/TiO_(x))catalysts were in-situ synthesized via the coprecipitation method to study the effect of rare earth metal Y doping on the structure of TiO_(2) and the catalytic performance for PDH.Experimental results demonstrate that Ydoped TiO_(2) exhibits higher catalytic activity,propylene selectivity and stability than bare TiO_(2).Full characterizations with X-ray diffraction(XRD),high-resolution transmission electron microscope(HRTEM),X-ray photoelectron spectroscopy(XPS),infrared spectroscopy of pyridine adsorption(Py-IR),temperature-programmed desorption of ammonia(NH_(3)-TPD),H_(2) temperature-programmed reduction(H_2-TPR),and Raman techniques on these catalysts reveal that Y^(3+)can enter TiO_(2) lattice,and the lattice stability of the catalyst can be enhanced by replacing Ti^(4+)to form Y-O-Ti structure.Meanwhile,the introduction of an appropriate amount of Y can obviously promote the PDH reaction by adjusting the acidity of the catalyst,improving the release capacity of TiO_(2) lattice oxygen and increasing the formation of active centers.Nevertheless,excessive Y doping will lead to pore clogging,and the exposure of active sites will be reduced,resulting in the degradation of catalytic performance.

关键词： Propane dehydrogenation Anatase Lattice defects Coordinatively unsaturated Ti cations Oxygen vacancy Rare earths

CsRAXs negatively regulate leaf size and fruiting ability through auxin glycosylation in cucumber

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Journal of Integrative Plant Biology 2024年第5期66卷 1024-1037页

作者： Jiacai Chen Liu Liu Guangxin Chen Shaoyun Wang Ye Liu Zeqin Zhang Hongfei Li liming Wang Zhaoyang zhou Jianyu Zhao Xiaolan Zhang Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops Department of Vegetable SciencesBeijing key Laboratoy of Growtn and Developmental Regul China Agricultural UniversityBeijing 100193China

Leaves are the main photosynthesis organ that directly determines crop yield and biomass.Dissecting the regulatory mechanism of leaf development is crucial for food security and ecosystem turn-over.Here,we identified the novel function of R2R3-MYB transcription factors CsRAXs in regulating cucumber leaf size and fruiting ability.Csrax5 single mutant exhibited enlarged leaf size and stem diameter,and Csrax1/2/5 triple mutant displayed further enlargement phenotype.Overexpression of CsRAX1 or CsRAX5 gave rise to smaller leaf and thinner stem.The fruiting ability of Csrax1/2/5 plants was significantly enhanced,while that of CsRAX5 overexpression lines was greatly weakened.Similarly,cell number and free auxin level were elevated in mutant plants while decreased in overexpression lines.Biochemical data indicated that CsRAX1/5 directly promoted the expression of auxin glucosyltransferase gene CsUGT74E2.Therefore,our data suggested that CsRAXs function as repressors for leaf size development by promoting auxin glycosylation to decrease free auxin level and cell division in cucumber.Our findings provide new gene targets for cucumber breeding with increased leaf size and crop yield.

关键词： auxin glycosylation CsRAXs CsUGT74E2 fruiting ability leaf size

In situ formed Mg(BH4)2 for improving hydrolysis properties of MgH2

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Journal of Magnesium and Alloys 2024年第3期12卷 1204-1214页

作者： Yongyang Zhu Mili Liu liming Zeng Yin Wang Daifeng Wu Rui Li Qing zhou Renheng Tang Fangming Xiao Institute of Resources Utilization and Rare Earth Development Guangdong Provincial Key Laboratory of Rare Earth Development and ApplicationGuangdong Academy of Sciences School of Materials Science and Engineering Guangdong Provincial Key Laboratory of Advanced Energy Storage Materials South China University of Technology

The hydrolysis of MgH2delivers high hydrogen capacity(15.2 wt%), which is very attractive for real-time hydrogen supply. However, the formation of a surface passivation Mg(OH)2layer and the large excess of H2O required to ensure complete hydrolysis are two key challenges for the MgH2hydrolysis systems. Now, a low-cost method is reported to synthesize MgH2@Mg(BH4)2composite via ball-milling MgH2with cheap and widely available B2O3(or B(OH)3). By adding small amounts of B2O3, the in-situ formed Mg(BH4)2could significantly promote the hydrolysis of MgH2. In particular, the MgH2–10 wt% B2O3composite releases 1330.7 mL·g-1H2(close to 80% theoretical hydrogen generation H2) in H2O and 1520.4 mL·g-1H2(about 95%) in 0.5 M MgCl2in 60 min at 26 ℃ with hydrolysis rate of 736.9 mL·g-1·min-1and 960.9 mL·g-1·min-1H2during the first minute of the hydrolysis, respectively. In addition, the MgCl2solution allows repeated use by filtering and exhibits high cycle stability(20 cycles), therefore leading to much reduced capacity loss caused by the excess H2O. We show that by introducing B2O3and recycling the 0.5 M MgCl2solution, the system hydrogen capacity can approach 5.9 wt%,providing a promising hydrogen generation scheme to supply hydrogen to the fuel cells.

关键词： Hydrolysis MgH 2 Mg(BH 4 ) 2 Hydrogen generation B 2 O 3 MgCl 2

Bio-inspired Leg Design for a Heavy-Duty Hexapod Robot

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Journal of Bionic Engineering 2022年第4期19卷 975-990页

作者： Haoyuan Yi Zhenyu Xu Xueting Xin Liming ZHOU Xin Luo State Key Laboratory of Digital Manufacturing Equipment and Technology Huazhong University of Science and TechnologyWuhan 430074China Research Institute Inner Mongolia First Machinery Group Co.LtdBaotou 014030China

The leg structure is crucial to the legged robot's motion performance.With the size and load of the legged robot increasing,the difficulty of leg design increases sharply.Inspired by biomechanics,this paper proposes a leg design approach based on effective mechanical advantage(EMA)for developing the heavy-duty legged robot.The bio-inspired design approach can reduce the demand for joint actuation forces during walking by optimizing the ratio relationship between the joint driving force and ground contact force.A dimensionless EMA model of the leg for the heavy-duty legged robot is constructed in this paper.Leg dimensions and hinge point locations are optimized according to the EMA and energy-optimal criterion.Based on the optimal leg structure,an electrically driven tri-segmented leg prototype is developed.The leg's joint hinge points are located near the main support line,and the load-to-weight ratio is 15:1.The leg can realize a swing frequency of 0.63 Hz at the stride length of 0.8 m,and the maximum stride length can reach 1.5 m.

关键词： Legged robot EMA Heavy-duty Bio-inspired

A non-viral gene therapy for melanoma by staphylococcal enterotoxin A

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Chinese Chemical Letters 2024年第5期35卷 325-329页

作者： Ling Yang Min Ren Jie Wang liming He Shanshan Wu Shuai Yang Wei Zhao Hao Cheng Xiaoming zhou Maling Gou State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan UniversityChengdu 610041China Department of Ophthalmology West China HospitalSichuan UniversityChengdu 610041China State Key Laboratory of Biotherapy Department of Integrated Traditional Chinese and Western MedicineRare Diseases CenterWest China HospitalSichuan UniversityChengdu 610041China

Staphylococcal enterotoxin A(SEA)derived from Staphylococcus aureus,as a superantigen,shows potential for cancer immunotherapy,but systemic immunotoxicity restricts its clinical application.Targeted delivery of SEA to tumor site provides a promising option for reducing the systemic toxicity.Here,we constructed an iRGD peptide(H-[Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys]-NH_(2))modified nanoparticle(iDPP)to deliver plasmids encoding SEA for melanoma treatment.The iDPP/SEA nanocomplexes efficiently mediated SEA expression in B16-F10 cells in vivo and in vitro and induced the activation of lymphocytes and maturation of murine bone marrow-derived dendritic cells(BMDCs)in vitro.In the subcutaneous B16-F10 melanoma model,the iDPP/SEA nanocomplexes could effectively enhance immune response and T lymphocytes infiltration in tumor site after intravenous administration,thereby considerably decreased melanoma growth.Meanwhile,no obvious adverse effect was observed after intravenous administration of the iDPP/SEA nanocomplexes in vivo.Our findings demonstrated that gene therapy of SEA is a potential candidate for melanoma treatment.

关键词： Gene therapy Superantigen Melanoma Staphylococcal enterotoxins A Immunotherapyene therapy

Elastic Buffering Layer on CuS Enabling High-Rate and Long-Life Sodium-Ion Storage

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Nano-Micro Letters 2022年第12期14卷 27-39页

作者： Yuanhua Xiao Feng Yue Ziqing Wen Ya Shen Dangcheng Su Huazhang Guo Xianhong Rui Liming ZHOU Shaoming Fang Yan Yu Key Laboratory of Surface and Interface Science and Technology Zhengzhou University of Light IndustryZhengzhou 450002People’s Republic of China Institute of Nanochemistry and Nanobiology School of Environmental and Chemical EngineeringShanghai UniversityShanghai 200444People’s Republic of China Institute School of Materials and Energy Guangdong University of TechnologyGuangzhou 510006People’s Republic of China Hefei National Research Center for Physical Sciences at the Microscale Department of Materials Science and EngineeringNational Synchrotron Radiation LaboratoryCAS Key Laboratory of Materials for Energy ConversionUniversity of Science and Technology of China.HefeiAnhui 230026People’s Republic of China

The latest view suggests the inactive core,surface pulverization,and poly sulfide shuttling effect of metal sulfides are responsible for their low capacity and poor cycling performance in sodium-ion batteries(SIBs).Whereas overcoming the above problems based on conventional nanoengineering is not efficient enough.In this work,erythrocyte-like CuS microspheres with an elastic buffering layer of ultrathin poly aniline(PANI) were synthesized through one-step selfassembly growth,followed by in situ polymerization of aniline.When CuS@PANI is used as anode electrode in SIBs,it delivers high capacity,ultrahigh rate capability(500 mAh gat 0.1 A g,and 214.5 mAh gat 40 A g),and superior cycling life of over 7500 cycles at 20 A g.A series of in/ex situ characterization techniques were applied to investigate the structural evolution and sodium-ion storage mechanism.The PANI swollen with electrolyte can stabilize solid electrolyte interface layer,benefit the ion transport/charge transfer at the PANI/electrolyte interface,and restrain the size growth of Cu particles in confined space.Moreover,finite element analyses and density functional simulations confirm that the PANI film effectively buffers the volume expansion,suppresses the surface pulverization,and traps the poly sulfide.

关键词： CuS Elastic buffering layer Polyaniline Long life Sodium-ion batteries

Status-quo of proved oil/gas initially-in-place in China as of 2020 and its variation trend in the past decade

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Energy Geoscience

Energy Geoscience 2022年第4期3卷 343-348页

作者： Liming ZHOU Daoyong Zhang Jinchao Sun Yufeng Gu Chenshuo Zhang Chen Zhang Strategic Research Center of Oil/gas Resources Ministry of Natural ResourcesBeijing100034China CHN Energy Lucency Enviro-Tech CO. LTDBeijing100039China

This study analyzes the status-quo of the proved oil/gas initially-in-place and its variation trend,the proved undeveloped oil/gas initially-in-place,and the remaining proved technically recoverable reserves(TRR)of oil/gas in China as of 2020 based on statistics.As shown by the results,the proved oil initially-in-place(OIIP),the proved undeveloped OIIP,and the remaining proved TRR of oil in China are mainly distributed in the Bohai Bay,Ordos and Songliao Basins,and those of free gas are mainly in the Ordos,Sichuan,and Tarim Basins.From 2011 to 2020,the largest increment in the proved OIIP,the proved undeveloped OIIP and the remaining proved TRR of oil occurred in the Ordos Basin,followed by the Bohai Bay Basin,while that in the proved gas initially-in-place(GIIP),the proved undeveloped GIIP,and the remaining proved TRR of gas occurred in the Ordos Basin,followed by the Sichuan Basin.In addition,a comprehensive analysis reveals that the petroliferous basins in China with the potential of reserve addition and production growth include the Ordos Basin,the Bohai Bay Basin,the Sichuan Basin,and the Tarim Basin.

关键词： Proved oil/gas initially-in-place(OIIP/GIIP) Proved undeveloped oil/gas initially-in-place(OIIP/GIIP) Remaining proved technically recoverable reserves(TRR) Petroleum distribution in Chinese basins Variation trend