A non-viral gene therapy for melanoma by staphylococcal enterotoxin A

作     者：Ling Yang Min Ren Jie Wang Liming He Shanshan Wu Shuai Yang Wei Zhao Hao Cheng Xiaoming Zhou Maling Gou Ling Yang;Min Ren;Jie Wang;Liming He;Shanshan Wu;Shuai Yang;Wei Zhao;Hao Cheng;Xiaoming Zhou;Maling Gou

作者机构：State Key Laboratory of Biotherapy and Cancer CenterWest China HospitalSichuan UniversityChengdu 610041China Department of OphthalmologyWest China HospitalSichuan UniversityChengdu 610041China State Key Laboratory of BiotherapyDepartment of Integrated Traditional Chinese and Western MedicineRare Diseases CenterWest China HospitalSichuan UniversityChengdu 610041China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2024年第35卷第5期

页      面：325-329页

核心收录：

学科分类：1002[医学-临床医学] 0703[理学-化学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation(No.82073363) the Sichuan Science and Technology Program(Nos.2020YFQ0059,2022YFQ0004) the Natural Science Foundation of Sichuan Province(No.2022NSFSC1304) 

主　　题：Gene therapy Superantigen Melanoma Staphylococcal enterotoxins A Immunotherapyene therapy 

摘      要：Staphylococcal enterotoxin A(SEA)derived from Staphylococcus aureus,as a superantigen,shows potential for cancer immunotherapy,but systemic immunotoxicity restricts its clinical *** delivery of SEA to tumor site provides a promising option for reducing the systemic ***,we constructed an iRGD peptide(H-[Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys]-NH_(2))modified nanoparticle(iDPP)to deliver plasmids encoding SEA for melanoma *** iDPP/SEA nanocomplexes efficiently mediated SEA expression in B16-F10 cells in vivo and in vitro and induced the activation of lymphocytes and maturation of murine bone marrow-derived dendritic cells(BMDCs)in *** the subcutaneous B16-F10 melanoma model,the iDPP/SEA nanocomplexes could effectively enhance immune response and T lymphocytes infiltration in tumor site after intravenous administration,thereby considerably decreased melanoma ***,no obvious adverse effect was observed after intravenous administration of the iDPP/SEA nanocomplexes in *** findings demonstrated that gene therapy of SEA is a potential candidate for melanoma treatment.