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Ames Test of Protein-modified Enterococcus faecalis

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Agricultural Biotechnology 2019年第1期8卷 78-79页

作者： lijun ding Shasha ZHAO Jiangsu Agri-animal Husbandry Vocational College

The potential mutagenicity of protein-modified Enterococcus faecalis was evaluated by the Ames test. The test subject was designed with 0. 05,0. 5,5. 0,and 50. 0 mg/dish doses,and a control group was set. With Salmonella typhimurium mutant strains TA97,TA98,TA100 and TA102 as test strains,the Ames test was carried out with( +) or without(-) S9,and the number of revertant colonies was counted. The results showed that for the protein-modified E. faecalis in the case of + S9 and-S9,the average numbers of revertant colonies of the four test strains were less than two times of that of the negative control group,and no dose-response relationship was observed. The protein-modified E. faecalis was tested to be negative in the Ames test,indicating that the test substance was not mutagenic in vitro.

关键词： Protein-modified Enterococcus faecalis Ames test

Diagnosis and Treatment of a Case of Canine Infectious Venereal Tumor

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Agricultural Biotechnology 2018年第6期7卷 101-102,109页

作者： Shasha ZHAO lijun ding Jiangsu Agri-animal Husbandry Vocational College Taizhou 225300China

Canine infectious venereal disease tumor( CTVT) is mainly due to a canine infectious intercourse tumor disease which is infected during *** is a malignant tumor originating from *** infection,the mucosal surface or prepuce of the urogenital tract is destroyed and bleed for a long time after being invaded by tumor tissue,which leads to inflammation of the urogenital tract and long-term blood loss,seriously affecting the health of *** this paper,the clinical diagnosis and treatment of a canine infectious venereal disease( STD) tumor are described,with emphasis on the course of chemotherapy for the disease.

关键词： Canine infectious venereal tumor Laboratory examination Chemotherapy

Teratogenic Effect of Paulownia Flower Extract on Rats

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Agricultural Biotechnology 2018年第4期7卷 100-101,105页

作者： lijun ding Yumei ZHANG Jiangsu Agri-animal Husbandry Vocational College Taizhou 225300China College of Veterinary Medicine Yangzhou UniversityYangzhou 225009China

In this study, Paulownia flower extract of different concentrations was administered to pregnant rats by gavage to investigate its teratogenic effect. It was found that Paulownia has neither teratogenic effect nor gro... 详细信息

关键词： Paulownia flower extract Rat Concentration Test

Rescue of Alcoholism in Dogs

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Animal Husbandry and Feed Science 2018年第5期10卷 295-296页

作者： ding lijun Zhao Shasha Jiangsu Agri-animal Husbandry Vocational College Taizhou 225300China

Under certain specific or unexpected circumstances, pet dogs ingested foods or drinks containing alcohol, and excessive alcohol would cause urinary incontinence or toxic syncope. Taking a case of canine alcoholism as an example, this paper expounds the diagnosis and treatment process of the disease, in order to provide references for veterinary workers.

关键词： Alcoholism Diagnosis Treatment Discussion

Diagnosis and Treatment of a Case of Gastroenteritis in Dog

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Agricultural Biotechnology 2018年第6期7卷 95-96页

作者： lijun ding Shasha ZHAO Jiangsu Agri-animal Husbandry Vocational College Taizhou 225300China

When dogs suffer from gastrointestinal inflammation,they will produce vomiting,diarrhea and dehydration and other symptoms,which are similar to canine distemper and canine *** symptoms are often confused with each other in *** the diagnosis and treatment of a case of canine gastroenteritis,this paper expounded the methods of differential diagnosis in detail,providing a reference for clinical diagnosis and treatment of the disease in pets.

关键词： Dog Gastroenteritis Diagnosis Treatment

In-vivo Mutagenicity of Protein-modified Enterococcus faecalis

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Agricultural Biotechnology 2019年第3期8卷 92-94页

作者： lijun ding Shasha ZHAO Jiangsu Agri-animal Husbandry Vocational College

The mutagenicity of the protein-modified Enterococcus faecalis was evaluated by a mouse bone marrow micronucleus test and a mouse sperm abnormality *** test substance was designed with three dose groups (1,2 and 5 g/kg·bw) and intragastrically administrated,with cyclophosphamide as a positive control and normal saline as a normal *** micronucleus rate and sperm abnormality rate were *** results showed that the micronucleus rates and sperm abnormality rates in the different dose groups were not significantly different from those in normal control group ( P >0.05),while the positive control group was significantly higher than the normal control group ( P <0.01).The conclusion is that the protein-modified *** was tested to be negative in both the mouse bone marrow micronucleus test and mouse sperm abnormality test,suggesting that it has no mutagenicity in vivo.

关键词： Protein-modified E.faecalis Mouse bone marrow micronucleus test Mouse sperm abnormality test

Diagnosis and Treatment of a Case of Radius and Ulna Fracture in Dog

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Agricultural Biotechnology 2019年第2期8卷 116-118页

作者： Shasha ZHAO lijun ding Jiangsu Agri-animal Husbandry Vocational College

60% of a dog's body weight is supported by its fore limbs, while the hind limbs mainly play the role of power, so the probability of dog's radius and ulna fracture is greatly increased. This paper mainly introduced the diagnosis and treatment of a case of radius and ulna fracture in dog, with an attempt to provide reference for clinical practice.

关键词： Radius and ulna fracture Diagnosis Treatment Postoperative care

Diagnosis and Treatment of a Case of Femoral Fracture in Dog

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Agricultural Biotechnology 2019年第2期8卷 122-123,164页

作者： lijun ding Shasha ZHAO Jiangsu Agri-animal Husbandry Vocational College

Femoral fracture of dogs is common in daily life. This paper introduced the diagnosis and treatment of a case of femoral fracture in dog, providing reference for clinical practice.

关键词： Femoral fracture Diagnosis Treatment Postoperative care

Umbilical cord artery-derived perivascular stem cells for treatment of ovarian failure through CD146 signaling

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Signal Transduction and Targeted Therapy 2022年第8期7卷 2694-2697页

作者： Lu Xu Yanjun Yang Lingling Zhang Guijun Yan Shiyuan Li Yifan Li Yali Hu lijun ding Bruno Péault Haixiang Sun Center for Reproductive Medicine and Obstetrics and Gynecology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Center for Molecular Reproductive Medicine Nanjing UniversityNanjing 210093China Department of Obstetrics and Gynecology the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China NHC Key Lab of Reproduction Regulation Shanghai Institute of Planned Parenthood ResearchPharmacy SchoolFudan UniversityShanghai 200032China Clinical Center for Stem Cell Research the Affiliated Drum Tower Hospital of Nanjing University Medical SchoolNanjing 210008China Orthopedic Hospital Research Center and Broad Stem Cell Center David Geffen School of MedicineUniversity of CaliforniaLos AngelesCAUSA State Key Laboratory of Pharmaceutical Biotechnology Nanjing UniversityNanjing 210008China

Dear Editor,Chemotherapy-induced ovarian failure significantly diminishes ovarian blood flow,ovarian size,and follicular *** plays a vital role in repairing ovarian damage.1 Perivascular stem cells(PSCs),known as mural cells covering the vasculature,are essential for blood vessel formation and postulated as progenitors of mesenchymal stem cells(MSCs).2,3 We previously established umbilical cord artery-derived PSCs(UCA-PSCs)and Wharton’s jelly-derived MSCs(WJ-MSCs)and UCAPSCs display optimal angiogenic capacity in vitro.4 Therefore,we explored the angiogenesis and pro-angiogenesis mechanisms of UCA-PSCs and provided them as an efficient treatment strategy for ovarian failure.

关键词： CD146 treatment artery damage