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The Crop Journal 2022年第3期10卷 911-916页

作者： Diaoguo An Guohao Han Jing Wang Hanwen Yan Yilin Zhou lijun cao Yuli Jin Xiaotian Zhang Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022HebeiChina The State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China

Rye(Secale cereale genome RR),a close relative of common wheat,possesses valuable resistance genes for wheat *** to the co-evolution of pathogen virulence and host resistance,some resistance genes derived from rye have lost *** and identification of new,effective resistance genes from rye is thus *** the current study,wheat-rye line WR56 was produced through distant hybridization,embryo rescue culture,chromosome doubling and ***56 was then proved to be a wheat-rye 2 RL ditelosomic addition line using GISH(genomic in situ hybridization),mc-FISH(multicolor fluorescence in situ hybridization),ND-FISH(non-denaturing FISH),mc-GISH(multicolor GISH)and rye chromosome arm-specific marker ***56 exhibited a high level of adult plant resistance to powdery mildew caused by Blumeria graminis ***(Bgt).This resistance was carried by the added 2 RL telosomes and presumed to be different from Pm7 which is also located on chromosome arm 2 RL but confers resistance at the seedling and adult ***56 will be a promising bridging parent for transfer of the resistance to a more stable wheat breeding line.A newly developed2 RL-specific KASP(kompetitive allele specific PCR)marker should expedite that work.

关键词： Blumeria graminis f.sp.tritici Disease resistance Ditelosomic line Secale cereale Triticum aestivum

Research on Nonlinear Dynamic Simulation and Fatigue Reliability Life Prediction for Synthesis Transmission System of Self-Propelled Gun

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Journal of Shanghai Jiaotong university(Science) 2018年第6期23卷 776-783页

作者： YU Guibo cao lijun WANG Shuhai MA Qiao Department of Gun Engineering Army Engineering UniversityShijiazhuang 050003China

Aiming to the puzzle that the inner load of nonlinear synthesis transmission system is difficult to obtain,a new kind of virtual prototype establishment and simulation method is put forward. The influence on nonlinear vibration with flexible rotor, bearing backlash is analyzed based on a virtual prototype. To validate the virtual prototype of nonlinear gear transmission system, the corresponding test platform is established. The consistency between simulation results and test results proves that the simulation results of the virtual prototype can be used to calculate the fatigue reliability life of key components. A new kind of fatigue reliability life prediction method of gear system considering multi-random parameter distribution is put forward based on the fatiguestatistic theory. Considering the periodicity of gear meshing, linear interpolation method is adopted to obtain the stress-time course of random load spectrum based on the gear's complicated torque provided by virtual *** gear's P-Sa-Sm-N curved cluster can be simulated based on material's P-S-N curve. The simulation process considers the parameter distributions of stress concentration coefficients, dimension coefficients and surface quality treatment coefficients, and settles the puzzle that traditional test methods cannot acquire the gear's fatigue life of all reliability levels. This method can provide the distribution function and the interval of fatigue reliability life of gear's danger region, and has a guide meaning for the gear maintenance periods determination and reliability evaluation.

关键词： synthesis transmission system virtual prototype nonlinear dynamic simulation fatigue reliability life prediction

Genome-wide characterization of microsatellites and development of polymorphic markers shared between two weevils of Eucryptorrhynchus(Coleoptera:Curculionidae)

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Zoological Systematics 2021年第4期46卷 273-280页

作者： Yujie Zhang Wei Song Jincui Chen lijun cao Junbao Wen Shujun Wei Beijing Key Laboratory for Forest Pest Control Beijing Forestry UniversityBeijing 100083China Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

The weevils Eucryptorrhynchus brandti(Harold)and ***(Motschulsky)are two pests of the tree of heaven,Ailanthus *** usually co-occur on their host ***,these two species have a niche separation with different eggs-laying locations and larvae-feeding parts on the ***,we characterized the distribution of microsatellites in the genome and developed genome-wide microsatellite markers shared between these two weevils.A total of 12,524 and 11,030 potential microsatellites were identified for *** and ***,*** with AT,TA,AC,TG,CA repeat motifs were most common for both *** designed primers universal for two weevils and developed 14 loci with high amplification efficacy and *** validated these microsatellite markers in three populations of each *** ***,the observed and expected heterozygosity for the genotyped loci ranged from 0.17-0.24 and 0.44-0.54;in ***,the above values ranged from 0.06-0.18 and *** on the developed microsatellite markers,we found that populations of both weevils have a clear pattern of genetic *** study provides valuable genetic markers for ecological,genetic,and evolutionary study of these two weevils.

关键词： Microsatellite primer population genetics

Mitochondrial gene rearrangement within genus Gasteruption(Hymenoptera: Evanioidea: Gasteruptiidae)

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Zoological Systematics 2016年第2期41卷 133-139页

作者： Shujun Wei lijun cao Qiuling Wu Chaodong Zhu Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences College of Agronomy and Plant Protection Qingdao Agricultural University Key Laboratory of Zoological Systematics and Evolution (CAS) Institute of Zoology Chinese Academy of Sciences

The complete mitochondrial genome of the Gasteruption parvicollarium Enderlein （GenBank accession number： KR270643） was sequenced in the study. Totally 17 009 bp sequence was determined with an A＋T content of 83.81%, including full set of typical animal mitochondrial genes. Two protein-coding and 10 tRNA genes as well as the A＋T-rich region were rearranged compared with the putative ancestral arrangement of insects. Most of the rearranged genes were located in the ancestral region of trnl-trnQ-trnM-nad2-trn W-trnC-trn Y- coxl-trnL2. The other rearranged genes are trnN and trnS1 located in the tRNA cluster trnA- trnR-trnN-trnSl-trnE-trnF and trnS2 located between cob and nadl. Remote inversion is dominant rearrangement event in G. parvicollarium mitochondrial genome, involving two protein-coding and 8 tRNA genes. Compared with the other mitochondrial genome reported in the same genus of Gasteruption, the inverted trnN was translocated to the tRNA cluster between cox1 and nad2 in G. parvicollarium. This is the first report of mitochondrial gene rearrangement occurred within genus of Hymenoptera. Our study points to a recently occurred gene rearrangement event in the Gasteruption species.

关键词： Apocrita wasp mitochondrial genome , gene rearrangement phylogeny

Surface Tension Calculation of Undercooled Alloys

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Rare Metals 2001年第1期20卷 58-61页

作者： Zhiyu Qiao, lijun Yan, Zhanmin cao, Guirong Liu (Department of Physical Chemistry, University of Science and Technology Beijing, Beijing 100083, China) 北京科技大学北京 100083

Based on the Butler equation and extrapolated thermodynamic data of undercooled alloys from those of liquid stable alloys, a method for surface tension calculation of undercooled alloys is proposed. The surface tensions of liquid stable and undercooled Ni-Cu (x(Ni)=0.42) and Ni-Fe (x(Ni)=0.3 and 0.7) alloys are calculated using STCBE (Surface Tension Calculation based on Butler Equation) program. The agreement between calculated values and experimental data is good enough, and the temperature dependence of the surface tension can be reasonable down to 150-200 K under the liquid temperature of the alloys.

关键词： undercooled alloy surface tension Butler equation

LION STATUES IN BEIJING

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China & The World Cultural Exchange 2008年第4期74卷 4-7页

作者： cao lijun

The lion is an exotic animal that was brought into inland China as an imperial tribute in the Han Dynasty (206 BC-220 AD) when Western influences began to be imported.

关键词：石狮雕像北京传统文化雕刻技术

Genome-wide developed microsatellite markers for the melon thrips Thrips palmi Karny(Thysanoptera:Thripidae)

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Zoological Systematics 2019年第2期44卷 100-110页

作者： Yongfu Gao Yajun Gong Ling Ma lijun cao Jincui Chen Min Chen Shujun Wei Beijing Key Laboratory for Forest Pest Control College of Forestry Beijing Forestry University Institute of Plant and Environmental Protection Beijing Academy of Agriculture and Forestry Sciences

The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtained randomly sequenced regions from the genome using next-generation sequencing and assembled 356 Mb genomic sequences. In total, 155,789 microsatellites were identified from the genomic sequences, of which, 64.02% were loci of dinucleotide repeat. Sixty primer pairs were initially validated in seven individuals. Thirty-five polymorphic markers were retained according to the amplification efficiency and *** 35 microsatellite loci were then assessed in 96 individuals in four geographical populations collected from China. The allele numbers ranged from 2 to 19 with an average value of 6.9 per locus, while the polymorphism information content ranged from 0.2219 to *** observed and expected heterozygosity varied from 0.5252 to 0.5367 and 0.5934 to 0.6148,respectively. Genetic structure was found between populations from southern and northern China. These novel microsatellite markers developed in our study provide abundant molecular markers for the genetic study of T. palmi.

关键词： Microsatellite genetic marker genome population genetic structure

ANCIENT PAGODAS IN BEIJING

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China & The World Cultural Exchange 2008年第2期74卷 24-27页

作者： cao lijun

Pagodas,an architectural type unique to Buddhist cultures,are widely distributed over the vast land of China,available in high mountains and deep valleys,ancient temples, snow-covered plateaus and the depth of deserts.

关键词：北京古塔建筑形式佛教文化

An Exhibition of Carved Stone Piers to Welcome Chinese New Year

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维普期刊数据库

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China & The World Cultural Exchange 2009年第3期75卷 32-35页

作者： Jia Kun & cao lijun

From January 24 to February 14, 2009, an exhibition oncarved stone piers from Beijing and Tianjin was held in Beijing International Sculpture Park, displaying nearly 300 pairs of traditional carved stone piers of the ... 详细信息

关键词：石刻图片展览桥墩民间传统码头博物馆清王朝摄影师

Beijing Ancient Observatory

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维普期刊数据库

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China & The World Cultural Exchange 2008年第11期74卷 34-37页

作者： Xin yuan cao lijun

The Ancient Observatory, a key cultural heritage site under national-level protection,is situated at the southeast corner of the cityproper of Beijing. Its east side was originally connected with the city wall of Beij... 详细信息

关键词：北京古代天文台文化继承权