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A post-GWAS confirming the genetic effects and functional polymorphisms of AGPAT3 gene on milk fatty acids in dairy cattle

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Journal of Animal Science and Biotechnology 2021年第1期12卷 122-140页

作者： lijun Shi Xin Wu Yuze Yang Zhu Ma Xiaoqing Lv Lin Liu Yanhua Li Feng Zhao bo Han Dongxiao Sun Department of Animal Genetics Breeding and ReproductionCollege of Animal Science and TechnologyKey Laboratory of Animal GeneticsBreeding and Reproduction of Ministry of Agriculture and Rural AffairsNational Engineering Laboratory for Animal BreedingChina Agricultural UniversityNo.2 Yuanmingyuan West RoadHaidian DistrictBeijing 100193China Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China Beijing General Station of Animal Husbandry Beijing 100101China Beijing Dairy Cattle Center Beijing 100192China.

Background: People are paying more attention to the healthy and balanced diet with the improvement of their living standards. Milk fatty acids(FAs) have been reported that they were related to some atherosclerosis and coronary heart diseases in human. In our previous genome-wide association study(GWAS) on milk FAs in dairy cattle, 83 genomewide significant single nucleotide polymorphisms(SNPs) were detected. Among them, two SNPs, ARS-BFGL-NGS-109493 and BTA-56389-no-rs associated with C18 index(P = 0.0459), were located in the upstream of 1-acylglycerol-3-phosphate O-acyltransferase 3(AGPAT3) gene. AGPAT3 is involved in glycerol-lipid, glycerol-phospholipid metabolism and phospholipase D signaling pathways. Hence, it was inferred as a candidate gene for milk FAs. The aim of this study was to further confirm the genetic effects of the AGPAT3 gene on milk FA traits in dairy ***: Through re-sequencing the complete coding region, and 3000 bp of 5′ and 3′ regulatory regions of the AGPA T3 gene, a total of 17 SNPs were identified, including four in 5′ regulatory region, one in 5′ untranslated region(UTR),three in introns, one in 3′ UTR, and eight in 3′ regulatory region. By the linkage disequilibrium(LD) analysis with Haploview4.1 software, two haplotype blocks were observed that were formed by four and 12 identified SNPs,respectively. Using SAS9.2, we performed single locus-based and haplotype-based association analysis on 24 milk FAs in 1065 Chinese Holstein cows, and discovered that all the SNPs and the haplotype blocks were significantly associated with C6:0, C8:0 and C10:0(P A, g.146704373 A > G, g.146704618 A > G and g.146704699 G > A) changed the transcription factor binding sites(TFBSs) for transcription factors SMARCA3, REX1, VMYB, BRACH, NKX26, ZBED4, SP1, USF1, ARNT and FOXA1. Out of them, two SNPs were validated to impact transcriptional ac

关键词： AGPAT3 Chinese Holstein Genetic effects Milk fatty acids Potential causal mutation

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依达拉奉右莰醇在高龄中重度急性缺血性脑卒中rt-PA静脉溶栓治疗中的应用时机

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山东医药 2024年第2期64卷 13-17页

作者：谷亚伟楚旭赵路静洪波罗芝宽林展增李强范宏光高静珍董银华王利军陈念南开大学附属第四中心医院神经内科天津300140 保定市徐水区人民医院内科南开大学附属第四中心医院急诊科天津市宁河区医院神经内科

目的探讨依达拉奉右莰醇在高龄中重度急性缺血性脑卒中(AIS)重组组织型纤溶酶原激活剂(rt-PA)静脉溶栓治疗中的应用时机。方法选择rt-PA静脉溶栓救治的221例高龄中重度AIS患者,随机分为对照组70例、早期组75例、晚期组76例。对照组接... 详细信息

目的探讨依达拉奉右莰醇在高龄中重度急性缺血性脑卒中(AIS)重组组织型纤溶酶原激活剂(rt-PA)静脉溶栓治疗中的应用时机。方法选择rt-PA静脉溶栓救治的221例高龄中重度AIS患者,随机分为对照组70例、早期组75例、晚期组76例。对照组接受rt-PA静脉溶栓治疗,早期组在rt-PA静脉溶栓治疗启动后即刻给予依达拉奉右莰醇治疗,晚期组在rt-PA静脉溶栓24 h后给予依达拉奉右莰醇治疗。于治疗7 d时采用NIHSS评分评价神经功能改善情况,治疗90 d时采用mRS评分评价预后情况,比较三组短期疗效和长期疗效;观察24 h症状性颅内出血发生率、14 d颅外系统性并发症发生率和90 d病死率,比较三组治疗安全性。结果三组7 d神经功能改善率、90 d预后良好率比较,早期组和晚期组均高于对照组,且早期组高于晚期组(P均0.05)。三组90 d病死率比较,早期组和晚期组均低于对照组,且早期组低于晚期组(P均<0.05)。结论高龄中重度AIS患者rt-PA静脉溶栓后即刻应用依达拉奉右莰醇可提高rt-PA静脉溶栓治疗的有效性和安全性,减少并发症的发生,改善患者预后。

关键词：依达拉奉右莰醇静脉溶栓急性缺血性脑卒中组织型纤溶酶原激活剂

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面向扩展目标跟踪的网格聚类量测划分方法

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智能系统学报 2022年第4期17卷 806-813页

作者：唐孟麒李波郝丽君辽宁工业大学电子与信息工程学院辽宁锦州121001

针对扩展目标跟踪中量测集划分困难及目标数目估计不准的问题,提出了一种面向扩展目标跟踪的网格聚类量测集划分方法。首先,由目标之间的时空关联性,将当前时刻的量测划分为存活目标量测与新生目标量测。然后,针对高斯混合概率假设密度... 详细信息

针对扩展目标跟踪中量测集划分困难及目标数目估计不准的问题,提出了一种面向扩展目标跟踪的网格聚类量测集划分方法。首先,由目标之间的时空关联性,将当前时刻的量测划分为存活目标量测与新生目标量测。然后,针对高斯混合概率假设密度滤波器与扩展目标高斯混合概率假设密度滤波器,分别推导出改进的模糊C均值算法与改进的网格聚类算法用于划分存活目标量测集与新生目标量测集。仿真结果表明本文方法可实现量测集的准确划分,有效完成扩展目标跟踪,避免了漏检与过检。

关键词：扩展目标量测集网格聚类时空关联模糊C均值存活目标新生目标概率密度假设

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Transcriptome analysis of Saposhnikovia divaricata and mining of bolting and fowering genes

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Chinese Herbal Medicines 2023年第4期15卷 574-587页

作者： Min Zhang Wenle Wang Qian Liu Erhuan Zang lijun Wu Guofa Hu Minhui Li Inner Mongolia Hospital of Traditional Chinese Medicine Hohhot 010020China Baotou Medical College Baotou 014040China Inner Mongolia Traditional Chinese&Mongolian Medical Research Institute Hohhot 010010China Hulunbuir Hengyi Chinese Medicinal Plants Co. Ltd.Hulunbuir 021099China

Objective: Early bolting of Saposhnikovia divaricata has seriously hindered its medicinal value and sustainable development of resources. The molecular mechanism of bolting and fowering of S. divaricata is still unclear and worth of research. In our study, we explored the transcriptome of the genes related to the bolting and fowering of S. ***: The transcriptome library was constructed, sequenced, assembled and annotated from the bolting and unbolting leaves of S. divaricata by high-throughput sequencing at the bud and fowering *** on the pathways related to bolting and fowering in plants, and exploring genes. The expression of seven candidate genes was verified by real-time fuorescence quantitative PCR(qRT-PCR).Results: Transcriptome results showed that 249 889 422 high-quality clean reads were obtained. A total of 67 866 unigenes were assembled with an average length of 948.1 bp. Trinity de Novo assembly produced 67 866 unigenes with an average length of 948.1 bp. Among 993 differentially expressed genes,484 genes were significantly up-regulated and 509 genes were down-regulated in the SdM group. A total of 79 GO terms were significantly enriched for differentially expressed genes. KEGG results showed that 11 154 unigenes were enriched in 89 pathways. And 21 candidate genes related to bolting and fowering of S. divaricata were excavated. The qRT-PCR results showed that expression trends of HDA9, PHYB, AP2,TIR1, Hsp90, CaM, and IAA7 were consistent with transcriptomic sequencing results. In addition, RNA-seq had identified 10 740 transcription factors and classified them into 58 families by their conserved domains. Further studies showed that the transcription factors regulating the fowering of S. divaricata were mainly distributed in the NAC, MYB_related, HB-other, ARF, and AP2 ***: Based on the results of this study, it was found that the plant hormone signal transduction pathway was one of the decisive factors to control bo

关键词： bolting and bloom comparative analysis flowering genes Saposhnikovia divaricata(Turcz.)Schischk transcriptome

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新型血液净化兔模型的建立及评价

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中华实验外科杂志 2024年第6期41卷 1333-1336页

作者：谷亚伟楚旭赵路静洪波罗芝宽林展增高静珍董银华王利军陈念张利霞潘思虎天津市第四中心医院神经内科天津300140 保定市徐水区人民医院内科保定072550 天津市第四中心医院急诊科天津300140 天津市宁河区医院神经内科天津301500 天津市胸科医院超声科天津300222 天津市环湖医院神经外科天津300222

目的探讨3种穿刺置管方式建立可持续血液净化治疗的新西兰兔模型的可行性及优劣性。方法120只雌性新西兰兔按照随机数字表法分为颌内静脉组、颈总动脉组、股动脉组和对照组,每组30只。采用22 Gauge密闭式防针刺伤型静脉留置针分组进行... 详细信息

目的探讨3种穿刺置管方式建立可持续血液净化治疗的新西兰兔模型的可行性及优劣性。方法120只雌性新西兰兔按照随机数字表法分为颌内静脉组、颈总动脉组、股动脉组和对照组,每组30只。采用22 Gauge密闭式防针刺伤型静脉留置针分组进行单侧颌内静脉穿刺置管、颈总动脉穿刺置管和股动脉穿刺置管。对照组不进行穿刺置管。记录单次穿刺置管时间、可置管次数,术后实验兔感染及死亡情况。比较4组实验兔的血常规、血糖、电解质及肝功能检测值。组间比较分别采用单因素方差分析、Kruskal-Wallis H检验及χ^(2)检验进行统计分析。结果3组均可成功构建动物模型。颌内静脉组的平均穿刺置管时间分别少于颈总动脉组和股动脉组[2.5(2.3,2.6)min比6.7(6.4,7.5)min、5.5(5.4,6.1)min,Z=67.110,P0.05)。颌内静脉组的术后白细胞数值分别低于颈总动脉组和股动脉组[(6.2±1.3)×10^(9)/L比(7.8±1.4)×10^(9)/L、(7.2±1.1)×10^(9)/L,F=14.571,P<0.01]。结论3种穿刺置管方式均可成功建立可持续血液净化治疗的新西兰兔模型,优选颌内静脉穿刺置管。

关键词：血液净化新西兰兔穿刺置管模型

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Rapid, automated, and reliable antimicrobial susceptibility test from positive blood culture by CAST‐R

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mLife 2022年第3期1卷 329-340页

作者： Pengfei Zhu Lihui Ren Ying Zhu Jing Dai Huijie Liu Yuli Mao Yuandong Li Yuehui He Xiaoshan Zheng Rongze Chen Xiaoting Fu Lili Zhang lijun Sun Yuanqi Zhu Yuetong Ji bo Ma Yingchun Xu Jian Xu Qiwen Yang Single‐Cell Center CAS Key Laboratory of BiofuelsShandong Key Laboratory of Energy Genetics and Shandong Energy InstituteQingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdaoChina University of Chinese Academy of Sciences BeijingChina College of Information Science&Engineering Ocean University of ChinaQingdaoChina Department of Clinical Laboratory Peking Union Medical College HospitalPeking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Graduate School Peking Union Medical CollegeChinese Academy of Medical SciencesBeijingChina Department of Clinical Laboratory Affiliated Hospital of Qingdao UniversityQingdao UniversityQingdaoChina Qingdao Single‐Cell Biotechnology Co.Ltd.QingdaoChina The Bioland Laboratory GuangzhouChina

Antimicrobial susceptibility tests(ASTs)are pivotal in combating multidrug resistant pathogens,yet they can be time‐consuming,labor‐intensive,and *** the AST of tigecycline for sepsis as the main model,here we establish an automated system of Clinical Antimicrobials Susceptibility Test Ramanometry(CAST‐R),based on D2O‐probed Raman mi*** a liquid robot for sample pretreatment and a machine learning‐based control scheme for data acquisition and quality control,the 3‐h,automated CAST‐R process accelerates AST by>10‐fold,processes 96 paralleled antibiotic‐exposure reactions,and produces high‐quality Raman *** Expedited Minimal Inhibitory Concentration via Metabolic Activity is proposed as a quantitative and broadly applicable parameter for metabolism‐based AST,which shows 99%essential agreement and 93%categorical agreement with the broth microdilution method(BMD)when tested on 100 Acinetobacter baumannii *** tests on 26 clinically positive blood samples for eight antimicrobials,including tigecycline,meropenem,ceftazidime,ampicillin/sulbactam,oxacillin,clindamycin,vancomycin,and levofloxacin reveal 93%categorical agreement with BMD‐based *** automation,speed,reliability,and general applicability of CAST‐R suggest its potential utility for guiding the clinical administration of antimicrobials.

关键词： antimicrobial susceptibility test laboratory automation Raman sepsis tigecycline

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Water-soluble PANI:PSS designed for spontaneous non-disruptive membrane penetration and direct intracellular photothermal damage on bacteria

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Bioactive Materials 2021年第12期6卷 4758-4771页

作者： Huanfeng Tang Yifan Liu Bing Li bo Shang Jiacheng Yang Congrou Zhang lijun Yang Kezheng Chen Wei Wang Jianfeng Liu Lab of Functional and Biomedical Nanomaterials College of Materials Science and EngineeringQingdao University of Science and TechnologyQingdao266042China Tianjin Key Laboratory of Radiopharmacokinetics for Innovative Drugs Chinese Academy of Medical SciencesAnd Institute of Radiation MedicineChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin300192PR China State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhou730000PR China

The major challenge in the field of antibacterial agents is to overcome the low-permeability of bacteria cell membranes that protects the cells against diverse *** this work,water-soluble polyaniline(PANI)-poly(p-styrenesulfonic acid)(PSS)(PANI:PSS)is found to spontaneously penetrate bacteria cellular membranes in a non-disruptive way,leaving no evidence of membrane poration/disturbance or cell death,thus avoiding side effects caused by cationic ammonia groups in traditional ammonia-containing antibacterial *** aqueous synthesis,which is important for biocompatibility,the polymer is synthesized via an enzyme-mimetic route relying on the catalysis of a *** to its fluorescent properties,the localization of as-prepared PANI:PSS is determined by the confocal microscope,and the results confirm its rapid entry into *** 808 nm near-infrared(NIR)irradiation,the internalized PANI:PSS generates local hyperthermia and destroys bacteria highly efficiently from inside the cells due to its excellent photothermal *** aureus(***),Methicillin-resistant Staphylococcus aureus(MRSA)and Escherichia coli(***)could be effectively eliminated as well as the corresponding bacterial *** of in vivo antibacterial experiments demonstrate excellent antibacterial activities of the water-soluble PANI:PSS without side ***,the prepared water-soluble polymer in this study has great potential in the treatment of various bacterial infections.

关键词： Conducting polymers Membrane penetration Photothermal Antibacterial

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基于65 nm体硅CMOS技术的DICE-DFF和TMR-DFF SEU辐射硬化方法分析

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电子科技大学学报 2022年第3期51卷 458-463页

作者：李海松杨博蒋轶虎高利军杨靓西安微电子技术研究所西安710065

基于65 nm体硅CMOS工艺,采用移位寄存器链方式对普通触发器(DFF)、2种双互锁触发器(DICE-DFF,FDICE-DFF)、普通触发器空间三模冗余(TMR-DFF)和2种普通触发器时间三模冗余(TTMR-DFF300,TTMR-DFF600)这6种结构进行单粒子翻转(SEU)性能试... 详细信息

基于65 nm体硅CMOS工艺,采用移位寄存器链方式对普通触发器(DFF)、2种双互锁触发器(DICE-DFF,FDICE-DFF)、普通触发器空间三模冗余(TMR-DFF)和2种普通触发器时间三模冗余(TTMR-DFF300,TTMR-DFF600)这6种结构进行单粒子翻转(SEU)性能试验评估。利用Ti、Cu、Br、I、Au和Bi这6种离子对被测电路进行轰击,试验结果表明,普通触发器单粒子翻转截面最大,约为3.5×10^(−8)~1.7×10^(−7) cm^(2)/bit;时钟间隔时间600 ps的时间三模冗余结构触发器单粒子翻转截面最小,约为5×10^(−11)~7×10^(−10)cm^(2)/bit,仅为普通触发器的0.1%左右。同时,针对6种触发器单元,从速度、面积、晶体管数量以及抗SEU性能多方面进行综合分析,为后续超大规模集成电路抗SEU设计提供了一定的指导意义。

关键词：翻转截面触发器双互锁触发器单粒子翻转三模冗余

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Does Financial Reporting Quality Affect Optimal Capital Structure？ Evidence from China

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Frontiers of Business Research in China 2016年第3期10卷 432-450页

作者： bo Zhang Hanze Wei lijun Ma School of Business Renmin Unive.rsity of China Beijing 100872 China

In this study, we investigate the association between financial reporting quality and a firm＇s deviation from its optimal capital structure. We find that a firm＇s deviation from its optimal capital structure is decreasing in financial reporting quality. The results suggest that high-quality financial reporting helps to reduce a firm＇s deviation from its optimal capital structure. In addition, we document that the relationship is intensified in non-SOEs, in which financial reporting quality plays a more crucial role. Furthermore, we find that bank connections diminish the role of financial reporting quality in optimal capital structure. Our results hold when we control for potential endogeneity in financial reporting quality and capital structure decisions. Our study contributes to both financial reporting quality and capital structure literature. We also contribute to the literature on informal contracts in organizational decision-making.

关键词： optimal capital structure financial reporting quality state-ownedenterprises bank connections

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Recruited CD68^(+)CD206^(+)macrophages orchestrate graft immune tolerance to prompt xenogeneic-dentin matrix-based tooth root regeneration

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Bioactive Materials 2021年第4期6卷 1051-1072页

作者： Hui Li Jingjing Sun Hefeng Yang Xue Han Xiangyou Luo lijun Liao bo Yang Tian Zhu Fangjun Huo Weihua Guo Weidong Tian State Key Laboratory of Oral Diseases West China Hospital of StomatologySichuan UniversityChengduChina National Engineering Laboratory for Oral Regenerative Medicine West China Hospital of StomatologySichuan UniversityChengduChina Department of Oral and Maxillofacial Surgery West China Hospital of StomatologySichuan UniversityChengduChina Department of Stomatology Beijing Shijitan HospitalCapital Medical UniversityBeijingChina Department of Stomatology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina Department of Dental Research The Affiliated Stomatological Hospital of Kunming Medical UniversityKunmingChina Department of Pediatric Dentistry West China Hospital of StomatologySichuan UniversityChengduChina

Successful regenerative medicine strategies of xenogeneic extracellular matrix need a synergistic balance among inflammation,fibrosis,and remodeling *** macrophage subsets have been identified to modulate inflammation and orchestrate the repair of neighboring parenchymal *** study fabricated PPARγ-primed CD68+CD206+M2 phenotype(M2γ),and firstly verified their anti-inflammatory and tissue-regenerating roles in xenogeneic bioengineered organ *** results showed that Th1-type CD3^(+)CD8^(+)T cell response to xenogeneic-dentin matrix-based bioengineered root complex(xeno-complex)was significantly inhibited by M2γmacrophage in ***γactivation also timely recruited CD68^(+)CD206^(+)tissue macrophage polarization to xeno-complex in *** subsets alleviated proinflammatory cytokines(TNF-α,IFN-γ)at the inflammation site and decreased CD3^(+)CD8^(+)T lymphocytes in the periphery *** translated to an orthotopic nonhuman primate model,PPARγ-primed M2 macrophages immunosuppressed IL-1β,IL-6,TNF-α,MMPs to enable xeno-complex to effectively escape immune-mediated rejection and initiate graft-host synergistic *** collective activities promoted the differentiation of odontoblast-like and periodontal-like cells to guide pulp-dentin and cementum-PDLs-bone regeneration and rescued partially injured odontogenesis such as DSPP and periostin ***,the regenerated root showed structure-biomechanical and functional equivalency to the native *** timely conversion of M1-to-M2 macrophage mainly orchestrated odontogenesis,fibrogenesis,and osteogenesis,which represents a potential modulator for intact parenchymal-stromal tissue regeneration of targeted organs.

关键词： Xenogeneic extracellular matrix Macrophage polarization Cellular response Organ regeneration Parenchymal and stromal tissues regeneration

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