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Journal of Molecular Cell Biology 2019年第5期11卷 371-382页

作者： Limin Liu Peng Zhang Ming Bai lijie he Lei Zhang Ting Liu Zhen Yang Menglu Duan Minna Liu Baojian Liu Rui Du Qi Qian Shiren Sun Department of Nephrology Xijing Hospital Fourth Military Medical University Xi'an 710032 China State Key Laboratory of Cancer Biology Fourth Military Medical University Xi'an 710032 China Department of Radiation Oncology Navy General Hospital Beijing 100048 China Department of Medicine Mayo Clinic College of Medicine and Mayo Graduate School Rochester MN 55905 USA

Hypoxia plays an important role in the genesis and progression of renal *** underlying mechanisms, however, have not been sufficiently elucidated. We examined the role of p53 in hypoxia-induced renal fibrosis in cell culture (human and rat renal tubular epithelial cells) and a mouse unilateral ureteral obstruction (UUO) model. Cell cycle of tubular cells was determined by flow cytometry, and the expression of profibrogenic factors was determined by RT-PCR, immunohistochemistry, and western blotting. Chromatin immunoprecipitation and luciferase reporter experiments were performed to explore the effect of HIF-lα on p53 expression. We showed that, in hypoxic tubular cells, p53 upregulation suppressed the expression of CDK1 and cyclins Bl and DI, leading to cell cycle (G2/M) arrest (or delay) and higher expression of TGF-β, CTGF, collagens, and fibronectin. p53 suppression by siRNA or by a specific p53 inhibitor (PIF-α) triggered opposite effects preventing the G2/M arrest and profibrotic changes. In vivo experiments in the UUO model revealed similar antifibrotic results following intraperitoneal administration of PIF-α(2.2 mg/kg). Using gain-of-function, loss-of-function, and luciferase assays, we further identified an HRE3 region on the p53 promoter as the HIF-lα-binding site. The HIF-la-HRE3 binding resulted in a sharp transcriptional activation of p53. Collectively, we show the presence of a hypoxia-activated, p53-responsive profibrogenic pathway in the kidney. During hypoxia, p53 upregulation induced by HIF-la suppresses cell cycle progression, leading to the accumulation of G2/M cells, and activates profibrotic TGF-β and CTGF-mediated signaling pathways, causing extracellular matrix production and renal fibrosis.

关键词： renal tubulointerstitial fibrosis hypoxia cell cycle (G2/M) arrest p53 HIF-lα TGF-β

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SJD581数字测井系统的故障诊断设计

西安电子科技大学学报 1989年第2期16卷 232-237页

作者：贺利洁西安电子科技大学电子工程系

本文讨论了一种数字系统的故障诊断的设计方法。在SJD581数字测井系统中,利用该方法可以把故障定位到30个区域之一,极大地方便了该系统的生产、调试和维修。

关键词：数学测井故障诊断故障模型

长链非编码RNA-MALAT1通过miR-320促进高糖诱导的人腹膜间皮细胞纤维化

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长链非编码RNA-MALAT1通过miR-320促进高糖诱导的人腹膜间皮细胞...

中国中西医结合学会肾脏疾病专业委员会2018年学术年会

作者： he lijie xijing hospital fourth military medical university

目的:探讨lnc RNA-MALAT1在人腹膜间皮细胞(HPMCs)高糖损伤导致的腹膜纤维化中的作用。方法:永生化的HPMCs采用50mmol/L高糖刺激培养不同时间后,使用real time-PCR及Western blot方法,检测MALAT1、可能的靶分子mi R-320、表型转换及纤...

目的:探讨lnc RNA-MALAT1在人腹膜间皮细胞(HPMCs)高糖损伤导致的腹膜纤维化中的作用。方法:永生化的HPMCs采用50mmol/L高糖刺激培养不同时间后,使用real time-PCR及Western blot方法,检测MALAT1、可能的靶分子mi R-320、表型转换及纤维化相关分子E-钙黏素(E-cadherin)、ɑ-平滑肌肌动蛋白(ɑ-SMA)、胶原蛋白I(collagenⅠ)、胶原蛋白Ⅲ(collagenⅢ)、纤连蛋白(fibronectin,FN)、结缔组织生长因子(Connective Tissue

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小城镇都市农业规划编制初探

国际城市规划 2015年第3期30卷 91-96页

作者：贺丽洁中国矿业大学(北京)力学与建筑工程学院

本文首先分析了小城镇都市农业规划编制工作的重要性;然后依据规划编制指南,阐明了小城镇都市农业的规划模式,同时把都市农业用地纳入到小城镇规划建设用地分类体系中,并制定出相关的技术指标和奖罚细则;最后建立评价体系来验证规划编... 详细信息

本文首先分析了小城镇都市农业规划编制工作的重要性;然后依据规划编制指南,阐明了小城镇都市农业的规划模式,同时把都市农业用地纳入到小城镇规划建设用地分类体系中,并制定出相关的技术指标和奖罚细则;最后建立评价体系来验证规划编制结果的优劣。

关键词：小城镇都市农业规划编制

Economic Development Status of the Countries along the Belt and Road and Their Correlations with Population and Carbon Emissions

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Journal of Resources and Ecology 2020年第6期11卷 539-548页

作者： ZHONG Yang LIN Aiwen ZHOU Zhigao he lijie YUAN Moxi School of Resource and Environmental Sciences Wuhan UniversityWuhan 430079China The Key Laboratory of Geographic Information System Wuhan UniversityWuhan 430079China Department of Geography and Resource Management The Chinese University of Hong KongHong Kong 999077China

The construction of the Silk Road Economic Belt and the 21st Century Maritime Silk Road are important measures for allowing China to expand its opening up to the outside world under the background of economic *** on this consideration,and from the perspective of geo-economics,this study uses a variety of mathematical statistical methods to analyze the economic development status and differences among the 30 countries along the Belt and *** addition,the correlations between GDP,population and carbon emissions in these countries are also *** results show that the current economic development levels of the countries along the Belt and Road are quite variable;the gaps between the indicators of the economic development of the countries along the Belt and Road are convergent;the GDP,population,and carbon emissions of the countries along the Belt and Road each showed an overall upward trend during the study period,and the changes in these three values showed significant *** all countries,the correlation coefficients between GDP and population(0.989),between GDP and carbon emissions(0.995),and between population and carbon emissions(0.993),all indicate that the correlations between GDP,population and carbon emissions are very *** them,GDP has the highest correlation with carbon emissions,reaching *** analysis shows that the value of R^2 reached 0.995,indicating that the regression fitting effect is very good and the calculation result is highly *** on these results,this paper proposes the following two suggestions:(1)Promoting the Belt and Road initiative should handle the relationship between developing and developed countries;and(2)Promoting the Belt and Road initiative should also be linked to China’s domestic regional development strategy.

关键词： the Belt and Road geo-economics correlation globalization China

Glycolytic dysregulation in Alzheimer's disease:unveiling new avenues for understanding pathogenesis and improving therapy

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Neural Regeneration Research 2025年第8期20卷 2264-2278页

作者： You Wu lijie Yang Wanrong Jiang Xinyuan Zhang Zhaohui Yao Department of Geriatrics Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina

Alzheimer's disease poses a significant global health challenge owing to the progressive cognitive decline of patients and absence of curative *** current therapeutic strategies,primarily based on cholinesterase inhibitors and N-methyl-Daspartate receptor antagonists,offer limited symptomatic relief without halting disease progression,highlighting an urgent need for novel research directions that address the key mechanisms underlying Alzheimer's *** studies have provided insights into the critical role of glycolysis,a fundamental energy metabolism pathway in the brain,in the pathogenesis of Alzheimer's *** in glycolytic processes within neurons and glial cells,including microglia,astrocytes,and oligodendrocytes,have been identified as significant contributors to the pathological landscape of Alzheimer's *** changes impact neuronal health and function,thus offering promising targets for therapeutic *** purpose of this review is to consolidate current knowledge on the modifications in glycolysis associated with Alzheimer's disease and explore the mechanisms by which these abnormalities contribute to disease onset and *** focus on the pathways through which glycolytic dysfunction influences Alzheimer's disease pathology should provide insights into potential therapeutic targets and strategies that pave the way for groundbreaking treatments,emphasizing the importance of understanding metabolic processes in the quest for clarification and management of Alzheimer's disease.

关键词： Alzheimer’s disease glial cells glycolysis neuronal metabolism pathogenesis therapeutic targets

Tessier连续提取法用于土壤铬分析的Cr(Ⅵ)-Cr(Ⅲ)转化及适用性

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环境工程学报 2021年第7期15卷 2368-2378页

作者：李东贺丽洁盛培培重庆大学煤矿灾害动力学与控制国家重点实验室重庆400044 重庆大学环境与生态学院重庆400044

为揭示Tessier连续提取法应用于土壤总铬(TCr)和六价铬[Cr(Ⅵ)]结合态分析的适用性,通过液相和土壤相实验,分别研究了提取液自身还原性组分及土壤残留还原剂在各个提取步骤中导致的Cr(Ⅵ)-Cr(Ⅲ)转化问题。结果表明,提取液中还原性组分... 详细信息

为揭示Tessier连续提取法应用于土壤总铬(TCr)和六价铬[Cr(Ⅵ)]结合态分析的适用性,通过液相和土壤相实验,分别研究了提取液自身还原性组分及土壤残留还原剂在各个提取步骤中导致的Cr(Ⅵ)-Cr(Ⅲ)转化问题。结果表明,提取液中还原性组分会在铁锰氧化物结合态和有机结合态的检测中导致Cr(Ⅵ)被还原为Cr(Ⅲ),可还原的Cr(Ⅵ)最大量分别为50.88和0.54 mg;经还原修复后的铬污染土壤中残留还原剂会在可交换态和碳酸盐结合态的检测中导致Cr(Ⅵ)的还原。提取过程中Cr(Ⅵ)-Cr(Ⅲ)的转化限制了Tessier连续提取法在铬污染土壤中的应用。当用于了解铬污染土壤TCr、Cr(Ⅵ)结合态的分布时,Cr(Ⅵ)铁锰氧化物结合态和有机结合态检测结果均显著低于真实值,TCr结合态的检测结果重现性较差。当用于评价修复后土壤中Cr(Ⅵ)结合态变化时,可交换态、碳酸盐结合态、铁锰氧化物结合态和有机结合态的检测结果均可能显著低于真实值。研究可为利用Tessier连续提取法准确评估铬污染土壤修复效果提供参考。

关键词： Tessier连续提取法铬污染土壤六价铬三价铬

NEAT1、miR-27a-3p在阿尔茨海默病患者血清和脑脊液中的表达关系

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北京大学学报（医学版） 2024年第2期56卷 207-212页

作者：何丽杰张春艳王静天津市第五中心医院检验科天津300450 天津市第五中心医院天津市早产儿器官发育表观遗传重点实验室天津300450

目的:探究长链非编码RNA核富含丰富的转录本1(long chain non-coding RNA nuclear-enriched abundant transcript 1, LncRNA NEAT1)、miR-27a-3p在阿尔茨海默病(Alzheimer disease, AD)患者血清和脑脊液中的表达关系及意义。方法:选择2019年10月至2021年9月天津市第五中心医院神经内科收治的AD患者66例作为病例组,根据临床痴呆评定量表(clinical dementia rating, CDR)评分分为轻度组(≤1分,n=41)与中重度组(>1分,n=25);另取同期门诊其他就诊患者血清和脑脊液标本66例志愿者作为对照组。收集所有受试者的一般资料并评估认知程度,采用实时荧光定量PCR检测血清和脑脊液miR-27a-3p、NEAT1表达水平,酶联免疫吸附试验检测脑脊液β-淀粉样前体蛋白裂解酶1(β-site amyloid precursor protein cleaving enzyme 1,BACE1)、β淀粉样蛋白(amyloid β,Aβ)40和Aβ42水平,采用Spearman法分析血清miR-27a-3p、NEAT1水平与简易精神状态检测量表的相关性,采用Pearson法分析血清miR-27a-3p、NEAT1水平与Aβ沉积平均标准摄取值比率(standardized uptake value ratio, SUVR)及脑脊液miR-27a-3p、NEAT1、BACE1、Aβ42、Aβ40水平的相关性。结果:MMSE评分[21 (17,25),9(7,11)vs. 27 (21,34)]、MoCA评分[17 (12,21),10 (7,13)vs. 27 (21,31)]、血清miR-27a-3p水平(0.55±0.13,0.46±0.06 vs. 0.97±0.22)、脑脊液miR-27a-3p(0.48±0.10,0.35±0.10 vs. 1.03±0.31)、Aβ42水平[(303.55±36.77) ng/L,(231.45±34.14) ng/L vs.(499.99±53.63) ng/L]及Aβ42/Aβ40比值(0.030±0.008, 0.022±0.007 vs. 0.048±0.010)轻度组、中重度组AD患者均低于对照组(P均<0.05),且中重度组AD患者较轻度组低(P均<0.05);血清NEAT1水平(2.31±0.64,3.13±0.76 vs. 1.05±0.20)、SUVR(1.50±0.29,1.76±0.52 vs. 0.74±0.15)及脑脊液NEAT1(3.51±1.24,4.30±1.65 vs. 1.01±0.23)、BACE1水平[(55.78±5.98)μg/L,(72.32±16.08)μg/L vs.(21.39±3.73)μg/L]轻度组、中重度组AD患者均高于对照组(P均<0.05),且中重度组AD患者较轻度组高(P均<0.05)。AD患者血清NEAT1水平与SUVR及脑脊液NEAT1、BACE1呈正相关(r=0.350,0.606,0.341,P<0.05),与MMSE评分、MoCA评分呈负相关(r=-0.473,-0.482,P均<0.05);血清miR-27a-3p水平与脑脊液miR-27a-3p水平、MMSE评分、MoCA评分呈正相关(r=0.695,0.424,0.412,P<0.05),与SUVR及脑脊液BACE1水平呈负相关(r=-0.521、-0.447,P均<0.05)。结论:NEAT1、miR-27a-3p在AD患者血清及脑脊液中表达趋势具有一致性,NEAT1水平均升高,miR-27a-3p水平均降低,二者水平呈负相关,与AD患者脑中Aβ沉积程度有关,并参与AD的病情进展。

关键词：阿尔茨海默病长链非编码RNA核富含丰富的转录本1 miR-27a-3p β淀粉样蛋白相关性

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“城市双修”理念下王平煤矿废弃地再生设计

工业建筑 2022年第12期52卷 19-26页

作者：贺丽洁刘佳妹中国矿业大学(北京)力学与建筑工程学院北京100083

矿产资源枯竭后形成的矿业废弃地严重威胁着周边地区的生态环境和可持续发展,对其进行再生利用是城市进行生态文明建设和转型发展的当务之急。以北京王平煤矿废弃地为研究对象,对其历史概况、矿区现状、外部发展机遇以及现有资源等进行... 详细信息

矿产资源枯竭后形成的矿业废弃地严重威胁着周边地区的生态环境和可持续发展,对其进行再生利用是城市进行生态文明建设和转型发展的当务之急。以北京王平煤矿废弃地为研究对象,对其历史概况、矿区现状、外部发展机遇以及现有资源等进行分析;基于“城市双修”理念,从生态恢复、场地规划、建筑更新、景观重构4方面进行再生更新设计,以期对我国矿业废弃地的再生利用提供借鉴与参考。

关键词：城市双修矿业废弃地王平煤矿