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Verification of Glyphosate Resistance, Lepidopteran Resistance and Wide Compatibility of Male Sterile Line E1C4008S in Rice

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Rice science 2020年第3期27卷 215-226页

作者： ZENG Qiang deng lihua HU Wenbin HE Xingjian MENG Qiucheng YU Jianghui LI Jinjiang WENG Lushui XIAO Guoying Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China University of Chinese Academy of Sciences Beijing 100049China Hunan Rice Research Institute Changsha 410125China The Sericultural Research Institute of Hunan Province Changsha 410125China The Innovation Academy for Seed Design Chinese Academy of SciencesBeijing 100101China

Weeds and insect pests are two important biotic stresses resulting in yield loss in rice, and wide compatibility is the essential characteristic of breeding inter-subspecific hybrid rice. This study focused on glyphosate resistance, lepidopteran resistance and wide compatibility as well as identification of molecular and some agronomic characteristics of transgenic male sterile line E1 C4008 S. The results indicated that glyphosate resistance gene Epsps^# and lepidopteran resistance gene Cry1 ca^# were transferred into japonica wide compatibility male sterile line 4008 S by Agrobacterium-mediated method, and four independent transformation events named E1 C4008 S-1, E1 C4008 S-2, E1 C4008 S-3 and E1 C4008 S-4 were obtained, in which E1 C4008 S-3 and E1 C4008 S-4 were of single copy insertion. The EPSPS protein contents of E1 C4008 S-3 and E1 C4008 S-4 in different organs were significantly different both in descending order of leaf > stem > root, and ranged from 300.58 to 1410.69 μg/g at the tillering stage. The glyphosate tolerable concentration(dosage) of E1 C4008 S-3 and E1 C4008 S-4 reached at least 16 g/L(54.42 kg/hm^2), and the seeds of E1 C4008 S-4 can germinate normally on the medium containing 1 g/L glyphosate. The CRY1 C protein contents of E1 C4008 S-3 and E1 C4008 S-4 in different organs were significantly different both in descending order of leaf > stem > root, and ranged from 0.62 to 2.43 μg/g at the tillering stage. The larvae mortalities of rice leaf rollers fed on leaves of E1 C4008 S-3 and E1 C4008 S-4 for 5 d were 95.35% and 97.77%, respectively, while the average mortalities of silkworms fed with protein extracts from leaves of E1 C4008 S-3 and E1 C4008 S-4 reached 94.55% and 83.64%, respectively. The results suggested that wide compatibility and evaluated agronomic traits of E1 C4008 S-4 were not significantly changed by insertion of the exogenous genes. Overall, a novel male sterile germplasm with glyphosate resistance, lepidopteran resistance

关键词： rice glyphosate resistance lepidopteran resistance wide compatibility male sterile line bioengineered crop

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A simple and efficient CRISPR/Cas9 system permits ultra-multiplex genome editing in plants

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The Crop Journal 2024年第2期12卷 569-582页

作者： Suting Wu Htin Kyaw Zhijun Tong Yirong Yang Zhiwei Wang Liying Zhang lihua deng Zhiguo Zhang Bingguang Xiao William Paul Quick Tiegang Lu Guoying Xiao Guannan Qin Xue'an Cui Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Biological Breeding for Fujian and Taiwan Crops Ministry of Agriculture and Rural AffairsCollege of AgricultureFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125HunanChina School of Biosciences The University of SheffieldFirth CourtWestern BankSheffield S102TNUK Key Laboratory of Tobacco Biotechnological Breeding National Tobacco Genetic Engineering Research CenterYunnan Academy of Tobacco Agricultural SciencesKunming 650021YunnanChina

The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic *** available genome-editing tools have a limited number of targets,restricting their application in genetic *** this study,we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors,eight donor vectors,four destination vectors,and one primer-design software *** combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sg RNA expression cassettes,the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci.A rice knockout vector containing 49 sg RNA expression cassettes was assembled and a high co-editing efficiency was *** plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering.

关键词： CRISPR/Cas9 Multiplex genome editing Assembly system Plant

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Resistance Evaluation of Indica Rice Varieties with Bacterial Blight Resistance Genes Xa21 and Xa23 under Different Genetic Backgrounds

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Plant Diseases and Pests 2022年第1期13卷 1-8,12页

作者： Jianghui YU Jinjiang LI lihua deng Lushui WENG Key Laboratory of Agro-ecological Processes in Subtropical Region Institute of Subtropical AgricultureChinese Academy of SciencesChangsha 410125China

[Objectives]The paper was to evaluate the resistance of indica rice varieties with bacterial blight resistance genes Xa21 and Xa23 under different genetic backgrounds.[Methods]The rice materials with bacterial blight resistance genes Xa21 or Xa23 were used as the donors,and those without the genes were used as the *** back crossing,composite crossing and other methods,combined with molecular marker-assisted selection(MAS),the materials with Xa21 or Xa23 gene,and their sister lines with/without Xa21 or Xa23 gene were inoculated with 7 widely used physiological races of Xanthomonas oryzae,and the resistance of the materials to bacterial blight was analyzed.[Results]The proportions of cumulative resistance(HR,R,MR)of rice materials with resistance gene Xa21 were HNA1-4/PXO86(84.62%)>YN24(82.14%)>GD1358(73.08%)>PXO99(67.86%)>GDA2(63.33%)>FuJ(6.67%),indicating that the materials with Xa21 gene had no resistance to bacterial blight induced by strain FuJ,but for other strains,there were still 36.67%-15.38%of the materials susceptible to the *** cumulative resistance(HR,R,MR)of the materials with Xa23 gene was over 76%,but there were still 23.02%-15.52%of the materials that had no resistance to the 7 *** analysis of variation coefficient of lesion length showed that the resistance of materials with Xa21 or Xa23 genes varied among strains,or the resistance to the same strain varied among *** analysis demonstrated that there was significant or extremely significant positive correlation between strain GDA2 or HNA1-4 and other 6 corresponding strains in the materials with Xa21 gene,and there was extremely significant positive correlation between strain YN24,GD1358 or PXO86 and other 6 corresponding strains in the materials with Xa23 *** of these strains for resistance identification could improve the efficiency of *** of sister lines showed that bacterial blight resistance of Xa21 or Xa23 genes was related to t

关键词： Rice Bacterial blight Resistance analysis Resistance gene

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Reduction of drosopterin content caused by a 45-nt insertion in Henna pre-mRNA of Drosophila melanogaster

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Science China(Life Sciences) 2008年第8期51卷 702-710页

作者： WANG Qin, ZHAO ChunJiang, BAI lihua, deng XueMei & WU ChangXin State Key Laboratory of Agrobiotechnology & Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China 1. State Key Laboratory of Agrobiotechnology & Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing 100193 China

Phenylalanine hydroxylase is assumed to be a key enzyme in drosopterin metabolism, but direct in vivo evidence to support this hypothesis is still *** the present study, we found a new natural reces-sive purple eye mutant of Drosophila melanogaster, Hnbp, which was a 45-nt insertion mutant in the second exon of *** insertion resulted in a predicted protein with 15 additional amino acids as compared to the wild-type *** analysis of protein structure showed that the predicted mutant protein probably had two more β-sheets, which may cause instability of two α-helices near the catalytic centre of the enzyme in the Biopterin-Hydroxyl binding *** mutant showed eye color defect with decrease of mRNA level, as well as drosopterin content *** drosopterin defect could be fully rescued by expression of wild type Henna in the Hnbp background by GMR-GAL4 UAS-Henna/UAS-Henna:Hnbp/Hnbp transgenic *** taken together, it can be concluded that the mu-tation in Henna is responsible for drosopterin reduction in mutant Hnbp, which provides key in vivo evidence to support the hypothesis that Henna is involved in drosopterin synthesis.

关键词： Drosophila melanogaster Henna gene drosopterin phenylalanine hydroxylase Hnbp

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可连续调谐的窄线宽外腔半导体激光器

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中国激光 2023年第23期50卷 253-256页

作者：邓力华阎柏屹梁伟中国科学技术大学纳米技术与纳米仿生学院安徽合肥230026 中国科学院苏州纳米技术与纳米仿生研究所江苏苏州215123 西交利物浦大学江苏苏州215123

可快速连续调谐的小尺寸窄线宽外腔半导体激光器是调频连续波(FMCW)激光雷达、量子技术等领域的核心器件。通过紧凑的反馈光路设计,采用腔长为2.5 mm的法布里-珀罗腔(FP腔)反馈压窄线宽,并结合压电陶瓷(PZT)进行腔长扫描,组装了波长为15... 详细信息

可快速连续调谐的小尺寸窄线宽外腔半导体激光器是调频连续波(FMCW)激光雷达、量子技术等领域的核心器件。通过紧凑的反馈光路设计,采用腔长为2.5 mm的法布里-珀罗腔(FP腔)反馈压窄线宽,并结合压电陶瓷(PZT)进行腔长扫描,组装了波长为1550 nm的小型化可调频的激光样机。采用光纤长度为1 m的马赫-曾德尔干涉仪,在重复频率为0.1、1.0、10.0、100.0 kHz下分别测得激光器无跳模的连续调谐范围为25.0、21.6、18.0、10.0 GHz。基于20 km长光纤的延时自外差法,测得3 dB线宽约为10 kHz。未来通过更加紧凑的光路设计,可以获得更大调频范围的超窄线宽激光。

关键词：激光器连续调频法布里-珀罗外腔半导体激光自注入锁定

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创业团队冲突导致创业失败的探索性研究

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科技进步与对策 2021年第17期38卷 134-143页

作者：孙继伟邓莉华上海大学管理学院上海200444

当前,创业团队冲突和创业失败研究文献较多,但研究两者关系的较少。选取创业团队冲突导致创业失败的4家企业作为典型案例,运用扎根理论研究方法,构建“输入-转化-输出”模型。其中,创业活动是输入部分,创业环境、创业团队冲突、冲突管... 详细信息

当前,创业团队冲突和创业失败研究文献较多,但研究两者关系的较少。选取创业团队冲突导致创业失败的4家企业作为典型案例,运用扎根理论研究方法,构建“输入-转化-输出”模型。其中,创业活动是输入部分,创业环境、创业团队冲突、冲突管理共同构成转化机制,创业失败是输出部分。在该理论模型框架下,深入挖掘创业团队冲突导致创业失败的演化路径,识别出创业活动“三阶段”和有效冲突管理的“两节点”,提出防范控制权争夺、减少决策分歧等一系列针对性建议。

关键词：创业团队冲突创业失败冲突管理扎根理论

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Exploring the interaction between the gut microbiota and cyclic adenosine monophosphate-protein kinase A signaling pathway:a potential therapeutic approach for neurodegenerative diseases

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Neural Regeneration Research 2025年第11期20卷 3095-3112页

作者： Fengcheng deng Dan Yang Lingxi Qing Yifei Chen Jilian Zou Meiling Jia Qian Wang Runda Jiang lihua Huang College of Pharmacy Chengdu University of Traditional Chinese MedicineChengduSichuan ProvinceChina State Key Laboratory of Southwestern Chinese Medicine Resources Chengdu University of Traditional Chinese MedicineChengduSichuan ProvinceChina

The interaction between the gut microbiota and cyclic adenosine monophosphate(cAMP)-protein kinase A(PKA)signaling pathway in the host's central nervous system plays a crucial role in neurological diseases and enhances communication along the gut–brain *** gut microbiota influences the cAMP-PKA signaling pathway through its metabolites,which activates the vagus nerve and modulates the immune and neuroendocrine ***,alterations in the cAMP-PKA signaling pathway can affect the composition of the gut microbiota,creating a dynamic network of microbial-host *** reciprocal regulation affects neurodevelopment,neurotransmitter control,and behavioral traits,thus playing a role in the modulation of neurological *** coordinated activity of the gut microbiota and the cAMP-PKA signaling pathway regulates processes such as amyloid-β protein aggregation,mitochondrial dysfunction,abnormal energy metabolism,microglial activation,oxidative stress,and neurotransmitter release,which collectively influence the onset and progression of neurological *** study explores the complex interplay between the gut microbiota and cAMP-PKA signaling pathway,along with its implications for potential therapeutic interventions in neurological *** pharmacological research has shown that restoring the balance between gut flora and cAMP-PKA signaling pathway may improve outcomes in neurodegenerative diseases and emotional *** can be achieved through various methods such as dietary modifications,probiotic supplements,Chinese herbal extracts,combinations of Chinese herbs,and innovative dosage *** findings suggest that regulating the gut microbiota and cAMP-PKA signaling pathway may provide valuable evidence for developing novel therapeutic approaches for neurodegenerative diseases.

关键词： cyclic adenosine monophosphate emotional disorders gut microbiota neurodegenerative diseases neurological diseases protein kinase A reciprocal regulation signaling pathway strategy therapies

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风险投资行业专长影响企业IPO审核吗?--来自中小板和创业板的经验证据

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会计与经济研究 2020年第2期34卷 77-93页

作者：徐虹邓李华刘家树安徽工业大学商学院安徽马鞍山243032 安徽工业大学安徽创新驱动发展研究院安徽马鞍山243032

文章选取2009-2016年深交所中小板和创业板申请IPO的企业为研究对象,探究风险投资行业专长对企业IPO审核通过率、IPO后长期市场业绩及财务业绩波动的影响。研究发现,高行业专长风险投资机构有助于提升企业IPO审核通过率,且这种提升作用... 详细信息

文章选取2009-2016年深交所中小板和创业板申请IPO的企业为研究对象,探究风险投资行业专长对企业IPO审核通过率、IPO后长期市场业绩及财务业绩波动的影响。研究发现,高行业专长风险投资机构有助于提升企业IPO审核通过率,且这种提升作用在熊市期间更显著;高行业专长风险投资机构对企业IPO后长期市场业绩有正向促进作用,有助于抑制企业IPO后财务业绩波动,且上述影响在熊市期间更显著。机制检验发现,高行业专长风险投资机构的优势主要体现在能更好地发挥增值服务和监督控制作用,在投资前的筛选企业能力方面并无明显优势。

关键词：风险投资行业专长 IPO审核长期市场业绩财务业绩波动

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外差探测随机相位调制连续波激光测距的快速解调方法

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光子学报 2023年第8期52卷 165-171页

作者：吴映邓力华马超峰梁伟中国科学院苏州纳米技术与纳米仿生研究所苏州215123

基于外差相干检测的随机相位调制连续波激光测距具有灵敏度高、对背景噪声容忍度高等优点,但传统的解调方法都是基于序列移位相乘和傅里叶变换,需要消耗大量计算资源和计算时间。提出并验证了一种针对随机相位调制连续波激光测距的快速... 详细信息

基于外差相干检测的随机相位调制连续波激光测距具有灵敏度高、对背景噪声容忍度高等优点,但传统的解调方法都是基于序列移位相乘和傅里叶变换,需要消耗大量计算资源和计算时间。提出并验证了一种针对随机相位调制连续波激光测距的快速解调方法,通过对回波外差信号进行傅里叶变换得到多普勒频移,解调出目标速度信息,然后通过数字下变频获得回波信号的正交分量,最后利用傅里叶变换在频域实现伪随机调制序列与回波信号的循环卷积,得到目标的位置信息。在原理验证实验中,使用长度为2 047的11阶伪随机二进制序列,调制速率为40 MHz,利用快速解调方法从回波信号中成功恢复了目标位置和速度,并在光纤中实现了2.7 m的距离分辨率。

关键词：激光测距相位调制相干探测伪随机码

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Sustainability of SARS-CoV-2 Induced Humoral Immune Responses in COVID-19 Patients from Hospitalization to Convalescence Over Six Months

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Virologica Sinica 2021年第5期36卷 869-878页

作者： Yang Zheng Qing Zhang Ashaq Ali Ke Li Nan Shao Xiaoli Zhou Zhiqin Ye Xiaomin Chen Shanshan Cao Jing Cui Juan Zhou Dianbing Wang Baidong Hou Min Li Mengmeng Cui lihua deng Xinyi Sun Qian Zhang Qinfang Yang Yong li Hui Wang Yake Lei Bo Yu Yegang Cheng Xiaolin Tong Dong Men Xian-En Zhang National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China Hubei Provincial Hospital of Traditional Chinese Medicine Wuhan 430074China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China Guang’an Men Hospital China Academy of Chinese Medical SciencesBeijing 100053China Health Bureau of Wuchang District Wuhan 430070China Hubei University Wuhan 430074China Shenzhen Mindray Bio-Medical Electronics Co. LtdShenzhen 518057China Hubei Provincial Center for Disease Control&Prevention Wuhan 430079China University of Chinese Academy of Sciences Beijing 100049China

Understanding the persistence of antibody in convalescent COVID-19 patients may help to answer the current major concerns such as the risk of reinfection,the protection period of vaccination and the possibility of building an active herd *** retrospective cohort study included 172 COVID-19 patients who were hospitalized in Wuhan.A total of404 serum samples were obtained over six months from hospitalization to *** in the specimens were quantitatively analyzed by the capture chemiluminescence immunoassays(CLIA).All patients were positive for the anti-SARS-Co V-2 Ig M/Ig G at the onset of COVID-19 symptoms,and the Ig G antibody persisted in all the patients during the ***,only approximately 25%of patients can detect the Ig M antibodies,Ig M against N protein(NIg M)and receptor binding domain of S protein(RBD-Ig M)at the 27 th *** titers of Ig M,N-Ig M and RBD-Ig M reduced to 16.7%,17.6%and 15.2%of their peak values *** contrast,the titers of Ig G,N-Ig G and RBD-Ig G peaked at 4–5 th week and reduced to 85.9%,62.6%and 87.2%of their peak values respectively at the end of *** behavior of antibodies and their correlation in age,gender and severity groups were *** general,the COVID-19 antibody was sustained at high levels for over six months in most of the convalescent *** a few patients with antibody reducing to an undetectable level which needs further *** humoral immune response against SARS-Co V-2 infection in COVID-19 patients exhibits a typical dynamic of acquired immunity.

关键词： Coronavirus disease 2019(COVID-19) Humoral Responses Sustainability Convalescent

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