Reduction of drosopterin content caused by a 45-nt insertion in Henna pre-mRNA of Drosophila melanogaster
Reduction of drosopterin content caused by a 45-nt insertion in Henna pre-mRNA of Drosophila melanogaster作者机构：State Key Laboratory of Agrobiotechnology & Key laboratory of Animal Genetics and Breeding of the Ministry of Agriculture College of Animal Science and Technology China Agricultural University Beijing China
出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)
年 卷 期：2008年第51卷第8期
页 面：702-710页
核心收录：
学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 071007[理学-遗传学]
基 金：the State Major Basic Research Development Program (Grant No. 2006CB102100) National Natural Science Foundation of China (Grant No. 30771535)
主 题：Drosophila melanogaster Henna gene drosopterin phenylalanine hydroxylase Hnbp
摘 要：Phenylalanine hydroxylase is assumed to be a key enzyme in drosopterin metabolism, but direct in vivo evidence to support this hypothesis is still *** the present study, we found a new natural reces-sive purple eye mutant of Drosophila melanogaster, Hnbp, which was a 45-nt insertion mutant in the second exon of *** insertion resulted in a predicted protein with 15 additional amino acids as compared to the wild-type *** analysis of protein structure showed that the predicted mutant protein probably had two more β-sheets, which may cause instability of two α-helices near the catalytic centre of the enzyme in the Biopterin-Hydroxyl binding *** mutant showed eye color defect with decrease of mRNA level, as well as drosopterin content *** drosopterin defect could be fully rescued by expression of wild type Henna in the Hnbp background by GMR-GAL4 UAS-Henna/UAS-Henna:Hnbp/Hnbp transgenic *** taken together, it can be concluded that the mu-tation in Henna is responsible for drosopterin reduction in mutant Hnbp, which provides key in vivo evidence to support the hypothesis that Henna is involved in drosopterin synthesis.
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