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Mining favorable alleles for five agronomic traits from the elite rapeseed cultivar Zhongshuang 11 by QTL mapping and integration

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The Crop Journal 2021年第6期9卷 1449-1459页

作者： Xianming Zhou lihong dai Pengfei Wang Ying Liu Zhaoqi Xie Haiyan Zhang Qiang Xin Lili Wan Liyong Yang Guangsheng Yang Dengfeng Hong National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Institute of Crops Wuhan Academy of Agricultural SciencesWuhan 430065HubeiChina Shanghai Academy of Agricultural Sciences Shanghai 201403China

Zhongshuang 11(ZS11) is an elite inbred rapeseed(Brassica napus L.) cultivar widely planted in the Yangtze River basin for its favorable characteristics including high seed oil content(SOC), low seed glucosinolate content(SGC), long siliques, and stable yield. To transfer the ideal traits from ZS11 into 195-14 A, a Polima(pol)-type cytoplasmic male sterile line with high general combining ability, a doubled haploid population derived from the cross of ZS11 and 195-14 A was developed. Based on this population,a high-density genetic linkage map covering 2553 c M with an average marker interval of 0.81 c M, was constructed using the Brassica 60 K SNP array and simple sequence repeats. In seven environments, 64,29, 35, 37, and 33 QTL were identified for silique length, seeds per silique, seed density per silique,SOC, and SGC, respectively. Most favorable alleles were from ZS11. Seventy-one consensus QTL were identified by a QTL meta-analysis, eight of which(cqSL–A9–2, cqSL–C7, cqSGC–C2, cqSOC–A5–2, cqSOC–A5–3, cqSPS–A6–2, cqSPS–A7–2, and cqSDPS–A9–2) were assigned as major QTL. Comparative genomics and expression analysis predicted 72 candidate genes underlying the 21 consensus QTL for the five *** findings suggest the genetic basis of the superior performance of ZS11 and suggest favorable alleles for development of cultivars with improved yield and quality. These results will assist in cloning these promising alleles in the future.

关键词： Rapeseed ZS11 QTL Five agronomic traits Candidate genes

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气相色谱法测定蔬菜中多种有机磷农药残留

食品安全导刊 2024年第24期 55-57页

作者：吴少青戴立红黄浩峰徐奕晖苏晓濛赵颖峰南京市产品质量监督检验院(南京市质量发展与先进技术应用研究院) 江苏南京210000

通过优化进样量、进样方式、温度程序和梯度设置等检测条件,建立了一种高效、可靠的气相色谱法检测蔬菜中多种有机磷农药残留的方法。实验结果显示,各有机磷农药在0.025~10.000μg·mL^(-1)具有良好线性关系,相关系数均大于0.999。加标... 详细信息

通过优化进样量、进样方式、温度程序和梯度设置等检测条件,建立了一种高效、可靠的气相色谱法检测蔬菜中多种有机磷农药残留的方法。实验结果显示,各有机磷农药在0.025~10.000μg·mL^(-1)具有良好线性关系,相关系数均大于0.999。加标回收率均在80%以上,相对标准偏差均小于5%。该方法具有较高的灵敏度、较好的准确性和重复性,可有效应用于蔬菜中有机磷农药残留的检测,为食品安全提供可靠的技术支持。

关键词：气相色谱蔬菜有机磷农药残留食品安全

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隘口沥青混凝土心墙堆石坝岩溶坝基稳定分析及处理研究

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岩石力学与工程学报 2006年第Z2期25卷 3802-3808页

作者：方朝阳代礼红段亚辉武汉大学水资源与水电工程科学国家重点实验室湖北武汉430072

研究岩溶坝基岩体稳定性评价方法和坝基处理措施,灌浆是一种可靠和有效提高地基防渗性能和地基稳定性的方法。根据隘口工程特点,确定基础灌浆处理方案,对不同岩溶分布情况下坝基岩体的稳定性进行分析比较,指出要保证沥青混凝土心墙的稳... 详细信息

研究岩溶坝基岩体稳定性评价方法和坝基处理措施,灌浆是一种可靠和有效提高地基防渗性能和地基稳定性的方法。根据隘口工程特点,确定基础灌浆处理方案,对不同岩溶分布情况下坝基岩体的稳定性进行分析比较,指出要保证沥青混凝土心墙的稳定和安全,必须对基础进行加固处理。根据计算结果确定不同深度坝基岩溶的处理方式:固结灌浆孔按3序逐渐加密加深,I序孔孔距12m,孔深13～36m,河床坝基处深36m,坝趾、坝踵和坝肩处13m;II序孔孔距6m,孔深13～21m,河床坝轴线处深21m,坝趾、坝踵及坝肩处深13m;III序孔孔距3m,孔深均为13m。对处理后的坝基岩体稳定性进行分析比较,计算分析表明,经灌浆处理后,沥青混凝土心墙的工作性状满足工程要求,基础处理方案是可行的。

关键词：水利工程岩溶灌浆法强度储备法沥青混凝土心墙堆石坝

Isolation and Identification of a Porcine Pseudorabies Virus Strain in Taizhou City

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Agricultural Biotechnology 2018年第5期7卷 133-135,142页

作者： Guangfu GUO Aiping ZHU Junping CAO Cailian JIN lihong dai Department of Veterinary Medicine Jiangsu Agri-animal Husbandry Vocational CollegeTaizhou 225300China Hubei Academy of Agricultural Sciences Wuhan 430064China

In this study, the liver, kidney and spleen tissues were collected from pigs with suspected PR in a pig farm in Jiangyan District, Taizhou City for virus isolation and identification. The isolated virus was inoculated onto PK15 monolayer cells. The virus culture was collected to extract genomic DNA for PCR assay and indirect immunoinfluscent assay. The results showed that the isolated virus was porcine pseudorabies virus, which was named TAIZ130417. The growth titer of the isolated virus reached 10 8.12 TCID 50 /ml on PK15 cells. Rabbits inoculated with the isolated virus soon exhibited pseudorabies symptoms such as itching and eventually died. The results provided reference for in-depth research and scientific prevention and control of pseudorabies.

关键词： Porcine pseudorabies virus Isolation Identification

中高污染风险水平大米无机成分分析标准物质研制

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化学分析计量 2024年第6期33卷 7-14页

作者：戴礼洪郝亚波姜红新赵玉杰肖笛王玉姣朱璇李黎徐亚平刘潇威农业农村部环境保护科研监测所农业农村部环境质量监督检验测试中心(天津)天津300191

研制了中高污染风险水平大米无机成分分析系列标准物质。按照JJF 1342—2022《标准物质研制(生产)机构通用要求》、JJF 1343—2022《标准物质的定值及均匀性、稳定性评估》等进行样品采集、加工制备、均匀性检验、稳定性检验,由11家不... 详细信息

研制了中高污染风险水平大米无机成分分析系列标准物质。按照JJF 1342—2022《标准物质研制(生产)机构通用要求》、JJF 1343—2022《标准物质的定值及均匀性、稳定性评估》等进行样品采集、加工制备、均匀性检验、稳定性检验,由11家不同行业的权威实验室进行联合定值,并对不确定度进行合成与评定。结果表明,样品粒度分布满足样品加工和检测的要求,具有良好的均匀性和稳定性。该系列大米无机成分分析标准物质共定值Pb、Cd、Cr、As、Cu、Zn、Ni、Mo、Fe、Mn、K、Ca、Mg、Na、Hg、Se 16种元素,定值数据为正态分布或近似正态分布,全部参数F实测值均小于F临界值[F0.05(24,50)=1.74],不同元素均匀性检验引入不确定度为0.20%~2.58%、稳定性检验引入不确定度为0.58%~4.79%、定值测试引入不确定度为0.67%~2.76%、扩展不确定度为6.45%~17.1%,量值准确可靠,不确定度范围评估合理,获批国家二级标准物质证书。研制的3种大米无机成分分析标准物质定值的元素含量梯度范围合理,解决了部分一级标准物质中Pb、Cr、Na、Hg、Se等缺少定值、难以进行计量溯源的问题,扩展了国内中高污染风险水平大米无机成分分析标准物质的范围。

关键词：大米中高风险水平无机成分分析标准物质

Isolation and Identification of Porcine Circovirus Type 2 in Taizhou

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Agricultural Biotechnology 2018年第4期7卷 108-109,249页

作者： Guangfu GUO Aiping ZHU Junping CAO Cailian JIN lihong dai Hongwei TAO College of Veterinary Medicine Jiangsu Agri-animal Husbandry Vocational CollegeTaizhou 225300China Hubei Academy of Agricultural Sciences Wuhan 430064China

In September 2011, an infectious disease suspected to be postweaning multisystemic wasting syndrome （PMWS） broke out in some pig farm in Taizhou. The inguinal lymph node, liver and lung tissues were collected and grinded into tissue suspension. The suspension was subjected to PCR detection, and the positive product was sequenced. The suspension of positive samples was filtered with 0.22 μm filter membrane, and the filtrate was inoculated onto PK15 cells. After five generations of blind passages, the cell viral liquid was collected and extracted for DNA, which was subjected to PCR detection and indirect immunofluorescence. The results showed that the isolate was porcine circovirus type 2 （PCV2） and designated as TAIZ110926. The target sequence was committed to NCBI with a serial number： KF039888.

关键词： Porcine circovirus type 2 Isolation Identification Target sequence

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过程控制编程方法在冷轧清洗线中的应用

清华大学学报（自然科学版） 2007年第Z2期47卷 1725-1728页

作者：戴立红陈雪波于政军辽宁科技大学电子与信息工程学院鞍山114044 鞍钢设计研究院鞍山114021

针对复杂的过程控制系统,采用可编程控制器(programmable logic controller,PLC)线性化编程方法,软件的开发和维护都很困难,甚至出现所开发的软件不可用的严重问题,往往不能满足客户需求。该文采用面向对象的方法对PLC过程控制系统进行... 详细信息

针对复杂的过程控制系统,采用可编程控制器(programmable logic controller,PLC)线性化编程方法,软件的开发和维护都很困难,甚至出现所开发的软件不可用的严重问题,往往不能满足客户需求。该文采用面向对象的方法对PLC过程控制系统进行程序设计,使得程序设计更加简单,设计的系统更加可靠,后续开发更加方便,并且利于代码复用。将此方法应用于鞍钢冷轧清洗线PLC过程控制中,使得大型PLC程序在集体合作设计时分工更加明确,现已投入实际运行,实践证明控制性能更加可靠,提高了带钢的质量,取得了一定的实用价值和经济价值。

关键词：可编程控制器面向对象冷轧清洗线

Lignans are the main active components of Schisandrae Chinensis Fructus for liver disease treatment:a review

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Food Science and Human Wellness 2024年第5期13卷 2425-2444页

作者： Ke Fu Shu dai Cheng Ma Yafang Zhang Shenglin Zhang Cheng Wang lihong Gong Honglin Zhou Yunxia Li State Key Laboratory of Southwestern Chinese Medicine Resources Key Laboratory of Standardization for Chinese Herbal MedicineMinistry of EducationSchool of PharmacyChengdu University of Traditional Chinese MedicineChengdu 611137China

As a traditional Chinese herbal medicine,Schisandrae Chinensis Fructus(SC)has been used in medicine and food industry due to its health care and therapeutic *** the past 20 years,the use of SC and its active ingredient lignans in the prevention and treatment of liver diseases has been increasing,and their hepatoprotective effects has increased the interest of the public and ***,in the present work,we first determined the effectiveness of SC in the treatment of liver diseases such as metabolic associated fatty liver disease,alcoholic liver disease,cholestatic liver disease and acute liver ***,the pharmacological effects and molecular mechanisms of lignans,the active components of SC,for liver disease treatment were comprehensively summarized for the first *** results showed that the lignans in SC could achieve hepatoprotective effects by regulating lipid metabolism,anti-fibrosis,anti-inflammation,anti-oxidation,anti-tumor and regulating bile acid *** mechanism mainly involved adenosine 5’-monophosphate-activated protein kinase,endoplasmic reticulum stress,sterol regulatory element binding protein 1c,autophagy,transforming growth factor-β,mitogen-activated protein kinase,microRNA,nuclear factor kappa-B,nuclear factor erythroid-2-related factor 2,heat shock proteins and pregnane X receptor signaling *** results can lay a scientific foundation for the development of hepatoprotective drugs or functional foods from SC/lignans.

关键词： Schisandrae Chinensis Fructus Lignans Hepatoprotection Pharmacological effects Molecular mechanism

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基于应力波衰减材料的目标层特征凸现方法

探测与控制学报 2018年第2期40卷 52-55,60页

作者：董灵飞戴黎红李蓉西安机电信息技术研究所陕西西安710065

针对多层侵彻过载粘连信号成分复杂、信号处理方法从中提取穿层特征压力大的问题,提出基于应力波衰减材料的目标层特征凸现方法。该方法采用灌封硅橡胶+纳米SiO_2颗粒制备的材料填充至引信壳体内侧,能够将弹体高速侵彻多层硬目标过程中... 详细信息

针对多层侵彻过载粘连信号成分复杂、信号处理方法从中提取穿层特征压力大的问题,提出基于应力波衰减材料的目标层特征凸现方法。该方法采用灌封硅橡胶+纳米SiO_2颗粒制备的材料填充至引信壳体内侧,能够将弹体高速侵彻多层硬目标过程中激发的应力波快速衰减,避免了应力波沿着弹体多次重复叠加造成侵彻过载的层间粘连,从而凸现侵彻过载的目标层特征。试验验证表明,同一发弹内采用该材料缓冲测得的侵彻过载层特征较未采用该材料缓冲的侵彻过载层特征明显。

关键词：高速侵彻多层硬目标过载信号粘连层特征凸现应力波衰减缓冲材料