Isolation and Identification of a Porcine Pseudorabies Virus Strain in Taizhou City

作     者：Guangfu GUO Aiping ZHU Junping CAO Cailian JIN Lihong DAI 

作者机构：Department of Veterinary MedicineJiangsu Agri-animal Husbandry Vocational CollegeTaizhou 225300China Hubei Academy of Agricultural SciencesWuhan 430064China 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2018年第7卷第5期

页      面：133-135,142页

学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：Supported by Youth Fund of Jiangsu Agri-animal Husbandry Vocational College(NSFQN1304) Key Project of Jiangsu Agri-animal Husbandry Vocational College(ZD201104) Phoenix Talent Project of Jiangsu Agri-animal Husbandry Vocational College(10434014001) 

主　　题：Porcine pseudorabies virus Isolation Identification 

摘      要：In this study, the liver, kidney and spleen tissues were collected from pigs with suspected PR in a pig farm in Jiangyan District, Taizhou City for virus isolation and identification. The isolated virus was inoculated onto PK15 monolayer cells. The virus culture was collected to extract genomic DNA for PCR assay and indirect immunoinfluscent assay. The results showed that the isolated virus was porcine pseudorabies virus, which was named TAIZ130417. The growth titer of the isolated virus reached 10 8.12 TCID 50 /ml on PK15 cells. Rabbits inoculated with the isolated virus soon exhibited pseudorabies symptoms such as itching and eventually died. The results provided reference for in-depth research and scientific prevention and control of pseudorabies.