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园艺学报 2024年第3期51卷 669-696页

作者：张正海王永富吴华茂于海龙曹亚从冯锡刚王立浩中国农业科学院蔬菜花卉研究所蔬菜生物育种全国重点实验室北京100081

概述了辣椒基因组公布近10年来,辣椒种内和种间遗传图谱构建,园艺性状、果实性状和抗病性状等重要性状遗传定位,全基因组关联分析和候选基因预测等研究进展。

关键词：辣椒遗传图谱全基因组关联分析混合分组分析法基因定位

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肝脏各“门”的解剖学发展及临床意义

医学综述 2024年第14期30卷 1717-1722页

作者：罗世超王利皓薛飞河南大学人民医院郑州大学人民医院河南省人民医院肝胆胰腺外科郑州450003

“肝门”作为进出肝脏管道结构的重要解剖概念,其理论范畴不断在肝脏手术实践中扩展、延伸,并出现了诸多肝脏的“门”理论,包括经典“肝门”(第一、二、三、四肝门)的概念、竜崇正教授提出的肝脏第三扇门、经由Laennec膜入路的肝脏各门(... 详细信息

“肝门”作为进出肝脏管道结构的重要解剖概念,其理论范畴不断在肝脏手术实践中扩展、延伸,并出现了诸多肝脏的“门”理论,包括经典“肝门”(第一、二、三、四肝门)的概念、竜崇正教授提出的肝脏第三扇门、经由Laennec膜入路的肝脏各门(GateⅠ~Ⅳ)等。这些概念在肝脏手术解剖中均具有重要价值,但关于“门”的理论、解剖依据和命名较复杂,且部分重叠易混淆。因此,全面了解肝脏解剖中“门”的概念和解剖特点,可以提高肝脏手术的安全性、精准性,为疾病的治疗提供新思路。

关键词：肝脏肝门解剖

Relationship between wall shear stresses and streamwise vortices in turbulent flows over wavy boundaries

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Applied Mathematics and Mechanics(English Edition) 2019年第3期40卷 381-396页

作者： lihao wang Weixi HUANG Chunxiao XU Lian SHEN Zhaoshun ZHANG Applied Mechanics Laboratory Department of Engineering MechanicsTsinghua University Department of Mechanical Engineering and Saint Anthony Falls Laboratory University of Minnesota

The relationship between wall shear stresses and near-wall streamwise vortices is investigated via a direct numerical simulation(DNS) of turbulent flows over a wavy boundary with traveling-wave motion. The results indicate that the wall shear stresses are still closely related to the near-wall streamwise vortices in the presence of a wave. The wave age and wave phase significantly affect the distribution of a two-point correlation coefficient between the wall shear stresses and streamwise vorticity. For the slow wave case of c/Um = 0.14, the correlation is attenuated above the leeward side while the distribution of correlation function is more elongated and also exhibits a larger vertical extent above the crest. With respect to the fast wave case of c/U_m=1.4, the distribution of the correlation function is recovered in a manner similar to that in the flat-wall case. In this case, the maximum correlation coefficient exhibits only slight differences at different wave phases while the vertical distribution of the correlation function depends on the wave phase.

关键词： direct numerical simulation(DNS) wall shear stress near-wall streamwise vortex two-point correlation

Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants

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Rice science 2019年第2期26卷 109-117,I0016,I0017页

作者： HE Yubing ZHU Min wang lihao WU Junhua wang Qiaoyan wang Rongchen ZHAO Yunde National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University College of Life Science and Technology Huazhong Agricultural University College of Plant Science & Technology Huazhong Agricultural University Section of Cell and Developmental Biology University of California San Diego

Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation and by backcross is laborious and time consuming. We previously reported the development of the transgene killer CRISPR(TKC) technology that uses a pair of suicide genes to trigger self-elimination of the transgenes without compromising gene editing efficiency. The TKC technology enables isolation of transgene-free CRISPR-edited plants within a single generation, greatly accelerating crop improvements. Here, we presented two new TKC vectors that show great efficiency in both editing the target gene and in undergoing self-elimination of the transgenes. The new vectors replaced the CaMV35 S promoter used in our previous TKC vector with two rice promoters to drive one of the suicide genes, providing advantages over our previous TKC vector under certain conditions. The vectors reported here offered more options and flexibility to conduct gene editing experiments in rice.

关键词： genome editing suicide gene transgene killer CRISPR Cas9 transgene-free

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河南省中医医院中医药文化现状及对策

中国中医药现代远程教育 2024年第19期22卷 206-206,I0001,I0002页

作者：宋文富王利豪关徐涛孙士玲河南中医药大学第一附属医院血液肿瘤科河南郑州450000

通过收集河南省中医医院中医药文化发展现状的基本资料,分析现有缺陷和不足,提出可行的对策和方法。在河南省中医医院中医药文化建设过程中,存在着文化融合度不高、缺乏自身特色、宣传不到位以及服务能力相对薄弱等问题。虽然有着许多... 详细信息

通过收集河南省中医医院中医药文化发展现状的基本资料,分析现有缺陷和不足,提出可行的对策和方法。在河南省中医医院中医药文化建设过程中,存在着文化融合度不高、缺乏自身特色、宣传不到位以及服务能力相对薄弱等问题。虽然有着许多独有的文化条件和深厚的历史背景,但部分中医医院并没有充分利用这些条件,导致医院建设以及中医药文化宣传都滞后于其他地区。因此河南省中医医院需要重新审视自己,发现自身问题和不足并加以改进,踏踏实实走出一条符合中医药文化特点的中医医院可持续发展道路。

关键词：中医医院中医药文化现状对策

Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants

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Molecular Plant 2018年第9期11卷 1210-1213页

作者： Yubing He Min Zhu lihao wang Junhua Wu Qiaoyan wang Rongchen wang Yunde Zhao National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China College of Plant Science & Technology Huazhong Agricultural University Wuhan 430070 China Section of Cell and Developmental Biology University of California San Diego La Jolla CA 92093-0116 USA

Dear Editor CRISPR gene-editing technology has successfully generated targeted mutations in rice and many other plant species （Ma et al., 2015）. Assessment of heritability and phenotypic stability of CRISPR-edited plants requires the elimination of the CRISPR construct. The presence of the CRISPR construct makes it difficult to distinguish the mutations transmitted from the previous generation from newly generated mutations by the CRISPR construct at the current generation. The existence of the CRISPR construct also greatly increases the risk of off-target effects.

关键词： MALE-STERILITY

Physiological Responses of Clam(Ruditapes philippinarum)to Transport Modes with Different Temperatures

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Journal of Ocean University of China 2023年第2期22卷 517-526页

作者： BI Shijie XUE Changhu XU Lili WEN Yunqi wang lihao LI Zhaojie LIU Hongying College of Food Science and Technology Hebei Agricultural UniversityBaoding 071000China College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Ocean College of Hebei Agricultural University Qinhuangdao 066000China

Given the increased circulation time after fishing,a series of changes take place in live clams,leading to a deterioration in quality even after ***,in this study,we aimed to explore the optimal mode of transportation of *** container for holding clams was reformed,and a water circulation temperature control system was *** physiological responses of clams during anhydrous and watery transportation at two temperatures(4 and 15℃)were investigated based on the aforementioned *** comparing the transportation patterns after 3 d of transport,a higher survival rate was observed at 4℃(97%)than at 15℃(63%)in the anhydrous transportation groups and a lower survival rate was observed at 4℃(93%)than at 15℃(99%)in the watery transportation *** addition,the glycogen content,condition index(CI),and adenylate energy charge(A.E.C)value were higher at4℃((40.87±0.99)mg g^(-1),13.71%±0.50%and 57.45%±1.60%)than at 15℃((30.54±0.81)mg g^(-1),9.09%±0.30%and 43.12%±1.65%)in the anhydrous transportation *** the watery transportation groups,a lower glycogen content,CI,and *** were observed at 4℃((33.78±0.84)mg g^(-1),9.78%±0.50%and 64.65%±1.25%)than at 15℃((41.53±0.93)mg g^(-1),12.72%±0.83%and 71.58%±1.27%).Results from this study show that anhydrous transportation(4℃)is the optimal transport condition for clams to maintain a high quality and good physiological ***,this study will be particularly useful for establishing shellfish transportation systems.

关键词： watery transportation anhydrous transportation clam physiological response temperature

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海南辣椒抗轻斑驳病毒病种质资源的筛选

分子植物育种 2024年第1期22卷 199-206页

作者：李雪峤于海龙伍壮生高芳华吴月燕王小娟王立浩海南省农业科学院蔬菜研究所海南省瓜菜育种工程技术研究中心海南省蔬菜生物学重点实验室海口571100 中国农业科学院蔬菜花卉研究所北京100081

为加速辣椒抗轻斑驳病毒病新品种的选育进程,有效应对近年来在海南辣椒生产中大面积发生的辣椒轻斑驳病毒病病情,通过IH1-04和L4-087H3T7R 2个分子标记筛选,结合病毒病苗期接种的方法,对已有的53份商品性较好的辣椒种质资源进行了抗轻... 详细信息

为加速辣椒抗轻斑驳病毒病新品种的选育进程,有效应对近年来在海南辣椒生产中大面积发生的辣椒轻斑驳病毒病病情,通过IH1-04和L4-087H3T7R 2个分子标记筛选,结合病毒病苗期接种的方法,对已有的53份商品性较好的辣椒种质资源进行了抗轻斑驳病毒病筛选,其中6份通过抗病筛选达到抗(R)级,包括泡椒1份、尖椒3份、线椒2份,且各辣椒资源的商品性普遍较好,加深了对抗病辣椒资源的分布情况,并为进一步辣椒抗轻斑驳病毒病辣椒新品种选育工作提供了切实基础。

关键词：辣椒轻斑驳病毒病(PMMoV) 分子标记苗期接种

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海南辣椒抗南方根结线虫种质资源的筛选

分子植物育种 2024年第18期22卷 6046-6052页

作者：李雪峤于海龙高芳华伍壮生王小娟吴月燕王立浩海南省农业科学院蔬菜研究所海南省瓜菜育种工程技术研究中心海南省蔬菜生物学重点实验室海口571100 中国农业科学院蔬菜花卉研究所北京100081

鉴于根结线虫的专化特性及其引发的植物病症防治的困难性与易复发性,选育并种植抗性品种仍然是目前最经济和最有效的防治策略之一,为加快辣椒抗南方根结线虫新品种的选育进程,有效应对近年来海南辣椒种植中大面积发生的南方根结线虫问题... 详细信息

鉴于根结线虫的专化特性及其引发的植物病症防治的困难性与易复发性,选育并种植抗性品种仍然是目前最经济和最有效的防治策略之一,为加快辣椒抗南方根结线虫新品种的选育进程,有效应对近年来海南辣椒种植中大面积发生的南方根结线虫问题,通过PM6a和PM542个分子标记筛选,结合苗期接种抗性鉴定的方法,对本单位已有的53份商品性较好的辣椒种质资源进行了抗南方根结线虫筛选,4份通过分子标记筛选,并在接种鉴定试验中抗性达到抗(R),其中包括泡椒1份,尖椒2份,线椒1份,且各辣椒资源的商品性较好,加深了对本单位抗性辣椒资源的分布情况,并为进一步抗南方根结线虫辣椒新品种选育工作提供了切实基础。

关键词：辣椒南方根结线虫(Meloidogyne incognita) 分子标记

Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing

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Journal of Genetics and Genomics 2017年第9期44卷 469-472页

作者： Yubing He Tao Zhang Ning Yang Meilian Xu Lang Yan lihao wang Rongchen wang Yunde Zhao National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Section of Cell and Developmental Biology University of California San Diego

Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop *** ability to cut DNA at specific locations in the genome to ... 详细信息

关键词： RGR Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing