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Decoding the fruit microbiome:A climate smart strategy to manage postharvest decays

Horticultural Plant Journal 2024年第5期10卷 1061-1072页

作者： Manori Kuruppu Kong lih Ling Phebe Ding Khairulmazmi Ahmad Asgar Ali Yasmeen Siddiqui Laboratory of Sustainable Agronomy and Crop Protection Institute of Plantation StudiesUniversiti Putra MalaysiaSerdang 43400Malaysia Department of Plant Protection Faculty of AgricultureUniversiti Putra MalaysiaSerdang 43400Malaysia Centre of Excellence for Post-harvest Biotechnology(CEFB) School of BiosciencesUniversity of Nottingham MalaysiaJalan Broga43500 SemenyihSelangorMalaysia Department of Biological Sciences College of ScienceKing Faisal UniversityAl Ahsa31982Saudi Arabia

There is an urgent necessity to shift our diets toward those rich in fruits and vegetables and at reduce postharvest losses of *** 20%-50%of fruits and vegetables are lost due to poor postharvest handling and pathogen infections in developing countries while it was estimated as 5%-35%in developed countries. Fresh fruits have evolved with a plethora of microorganisms having important roles in maintaining fruit health. However, little information is available on the dynamics, structure, and functional capacities of underpinning fruit microbiomes. The present review discussed environmental conditions favoring fruit-harbored antagonists and their different modes of action for suppressing postharvest pathogens in fruits. it also provides information on omics technologies such as next-generation sequencing (NGS),metaproteomics, metatranscriptomic, and metabolomics studies to characterize fruit microbiomes. With the advent of NGS and meta-omics technologies, microbiome research could bring remarkable development and understanding in succeeding biological treatments. in addition, they may provide us with a fundamental understanding of microclimate requirements for fruit microbiome establishment and microbiome shifts during post-harvest storage, which would be advantageous in developing composite biocontrol treatments for post-harvest decay management.

关键词： Biological control Food loss Fruit microbiome Meta-omics Microclimate Postharvest decay

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中空玻璃微球/TPU复合泡沫的制备及微孔结构的研究

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精密成形工程 2024年第6期16卷 24-33页

作者：梁议心王心超刘佳佳肖寒池耿铁 lih-Sheng Turng 河南工业大学机电工程学院郑州450001 威斯康星大学麦迪逊分校美国麦迪逊市53706

目的探讨GB含量变化对中空玻璃微球/TPU复合材料相变行为、流变性能及热力学性能的影响,并研究微米尺寸填料GB对泡沫微孔形态、尺寸分布的影响。方法以中空玻璃微球和热塑性聚氨酯为原料,采用微孔注塑成型(MiM)发泡方式,制备GB/TPU复合... 详细信息

目的探讨GB含量变化对中空玻璃微球/TPU复合材料相变行为、流变性能及热力学性能的影响,并研究微米尺寸填料GB对泡沫微孔形态、尺寸分布的影响。方法以中空玻璃微球和热塑性聚氨酯为原料,采用微孔注塑成型(MiM)发泡方式,制备GB/TPU复合泡沫材料。结果通过向TPU中引入微米玻璃球相改善发泡材料的微孔结构,在TPU中加入有机改性的GB,GB作为成核剂能显著提高TPU发泡材料的微孔结构。当GB质量分数为0.5%时,TPU熔体黏度得到提高,发泡性能得到改善,成功制备出具有复孔结构的GB/TPU复合泡沫。GB/TPU复合泡沫的密度被降低至0.51 g/cm^(3)。由于微米尺寸填料GB的平均直径为18μm,与TPU熔体形成较大的相界面,因此引起泡孔尺寸较大且泡孔开孔率较高的现象。结论差示扫描量热仪(DSC)测试结果表明,在冷却阶段及第二次加热熔融阶段,GB对TPU软段相和硬段相的相变行为没有明显的影响。但是在DSC第一次加热熔融阶段,GB引起了TPU熔融峰向低温区移动。旋转流变仪测试分析结果表明,在高剪切频率下,GB/TPU复合材料的复合黏度和储能模量明显高于TPU纯料的,且TPUGB0.5(含质量分数为0.5%的GB)的复合黏度和储能模量最大。TGA结果显示,热稳定性提高,利用扫描电子显微镜(SEM)观察复合材料泡沫材料泡孔结构可知,泡孔分布和尺寸均匀性显著提高。

关键词：中空玻璃微球(GB) 热塑性聚氨酯(TPU) 泡孔复合材料微孔结构

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Synthesis and Luminescent Properties of Planar-tip and Tapered-tip ZnO Nanorod Arrays

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Journal of Materials Science & Technology 2008年第4期24卷 633-636页

作者： Chun-wen wang Jr-hau HE lih-juann CHEN Department of Materials Science and Engineering National Tsing-Hua University Hsinchu City 30013 Taiwan China institute of Photonics and Optoelectronics and Department of Electrical Engineering National Taiwan UniversityTaipei 106 Taiwan China

Vertically aligned ZnO nanorods were synthesized on a-plane sapphire via a metal catalyzed vapor phase transport and condensation process in a two-zone vacuum furnace. Planar-tip and tapered-tip ZnO nanorods were successfully synthesized by utilizing different source materials under the same growth conditions. The growth mechanisms were proposed to be vapor-liquid-solid （VLS） process for planar-tip ZnO nanorods and a combination of VLS and self-catalyzed processes for tapered-tip ZnO nanorods, From cathodoluminescence （CL） measurements, tapered-tip ZnO nanorods have more intense green emission than planar-tip ZnO nanorods, and therefore possess higher oxygen vacancy concentration than planar-tip ZnO nanorods. From CL characteristics, well-aligned planar-tip ZnO nanorods shall serve effectively as laser source, while well-aligned tapered-tip ZnO nanorods are suitable for direction-related optical applications.

关键词： ZnO Well-aligned Nanorods Cathodoluminescence

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Antacid effects of Chinese herbal prescriptions assessed by a modified artificial stomach model

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World Journal of Gastroenterology 2010年第35期16卷 4455-4459页

作者： Tsung-Hsiu Wu i-Chin Chen lih-Chi Chen Chinese Medicine Pharmacy Department E-Da Hospital School of Chinese Medicine for Post-Baccalaureate I-Shou University Department of Pharmacy Taipei City Hospital College of Pharmacy Taipei Medical University

AiM:To assess the antacid effects of the tonic Chinese herbal prescriptions,Si-Jun-Zi-Tang(SJZT) and Shen-Ling-Bai-Zhu-San(SLBZS).METHODS:Decoctions of the tonic Chinese herbal prescriptions,SJZT and SLBZS,were prepared according to Chinese original *** pH of the prescription decoctions and their neutralizing effects on artif icial gastric acids were determined and compared with water and the active controls,sodium bicarbonate and colloidal aluminum phosphate.A modified model of Vatier's artificial stomach was used to determine the duration of consistent neutralization effect on artificial gastric *** neutralization capacity in vitro was determined with the titration method of Fordtran's ***:The results showed that both SJZT and SLBZS have antacid effects in *** with the water group,SJZT and SLBZS were found to possess signif icant gastric acid neutralizing *** duration for consistent neutralization of SLBZS was signif icantly longer than that of ***,SLBZS and SJZT exhibited signif icant antacid capacities compared to ***:SJZT and SLBZS were consistently active in the artificial stomach model and are suggested to have antacid effects similar to the active control drugs.

关键词： Traditional Chinese medicines Antacid Acid neutralizing capacity Artificial stomach

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Quantitative interactomic of CD40 in primary B cells

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hLife 2024年第2期2卷 88-93页

作者： Jeremy Argenty Emilie Maturin Mylene Camus Valentin Mellado Jeanne Perroteau Benoit Pasquier Guillaume Voisinne Odile Burlet-Schiltz lih-Ling Lin Anne Gonzalez de Peredo Romain Roncagalli Bernard Malissen Centre d’immunologie de Marseille-Luminy Aix Marseille UniversitéINSERMCNRSMarseilleFrance Centre d’immunophénomique Aix Marseille UniversitéINSERMCNRS UMRMarseilleFrance institut de Pharmacologie et de Biologie Structurale Département Biologie Structurale BiophysiqueProtéomique Génopole Toulouse Midi Pyrénées CNRS UMR 5089205 Route de NarbonneToulouse CedexFrance Checkpoint immunology Immunology and Inflammation Therapeutic AreaSanofiVitryFrance Checkpoint immunology Immunology and Inflammation Therapeutic AreaSanofiCambridgeUSA

iNTRODUCTiON Over the past few decades,antibody-based therapies have emerged as an additional tool to treat patients developing cancer and auto-immune *** natural properties of the developed antibodies may lead to agonist or blocking functions for the targeted ***,antibodies developed for clinical trials are extensively analyzed in vitro and in vivo for safety and efficacy,yet they are not routinely evaluated for their potential signaling effectiveness.

关键词： routine antibodies clinical

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A biomimetic basementmembrane consisted of hybrid aligned nanofibers andmicrofibers with immobilized collagen iV and laminin for rapid endothelialization

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Bio-Design and Manufacturing 2021年第2期4卷 171-189页

作者： Chenglong Yu Guoping Guan Stefanie Glas Lu wang Zhutong Li lih-Sheng Turng Key Laboratory of Textile industry for Biomedical Textile Materials and Technology College of TextilesDonghua University2999 North Renmin RoadSongjiang DistrictShanghai 201620China Engineering Research Center of Technical Textiles of Ministry of Education College of TextilesDonghua UniversityShanghai 201620China Wisconsin institute for Discovery University of Wisconsin-Madison330 North Orchard StreetMadisonWI 53715USA Department of Mechanical Engineering University of Wisconsin-Madison1513 University AveMadisonWI 53706USA

Rapid formation of a continuous endothelial cell(EC)monolayer with healthy endothelium function on the luminal surface of vascular implants is imperative to improve the longtime patency of small-diameter vascular *** the present study,we combined the contact guidance effects of aligned nanofibers,which enhance EC adhesion and proliferation because of its similar fiber scale with native vascular basement membranes,and aligned microfibers,which could induce EC elongation effectively and allow ECs *** was followed by successive immobilization of collagen iV and laminin to fabricate a biomimetic basement membrane(BBM)with structural and compositional *** hemolysis assay and platelet adhesion results showed that the BBM exhibited excellent ***,the adhered human umbilical vein endothelial cells(HUVECs)onto theBBMaligned along the orientation of the microfibers with an elongated morphology,and the data demonstrated that the BBM showed favorable effects on EC attachment,proliferation,and *** oriented EC monolayer formed on the BBM exhibited improved antithrombotic capability as indicated by higher production of nitric oxide and prostacyclin(PGi2).Furthermore,fluorescence images indicated that HUVECs could infiltrate into the BBM,implying theBBM’s ability to enhance transmural ***,theBBMpossessed the properties to regulate ECbehaviors and allow transmural ingrowth,demonstrating the potential to be applied as the luminal surface of small-diameter vascular implants for rapid endothelialization.

关键词： Biomimetic basement membranes Aligned electrospun fibers Surface modification Endothelialization Anti-thrombogenicity Transmural ingrowth

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Al⁃lih复合燃料制备及性能

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含能材料 2024年第1期32卷 12-19页

作者：王芳张鑫鹏王鼎程李春涛孙鑫科冯勇李伟航天化学动力技术重点实验室湖北襄阳441003 湖北航天化学技术研究所湖北襄阳441003

为了提高铝粉燃烧效率,利用球磨法分别制备了质量含量为3%,5%,10%,15%的Al‐lih复合燃料。通过X射线衍射仪(XRD)、扫描电镜(SEM)、粒度仪对样品结构、形貌和粒径进行表征;采用差热‐热重量热仪对其热氧化性能进行表征,最后采用CO_(2)激... 详细信息

为了提高铝粉燃烧效率,利用球磨法分别制备了质量含量为3%,5%,10%,15%的Al‐lih复合燃料。通过X射线衍射仪(XRD)、扫描电镜(SEM)、粒度仪对样品结构、形貌和粒径进行表征;采用差热‐热重量热仪对其热氧化性能进行表征,最后采用CO_(2)激光点火装置、高速摄像机及氧弹量热仪对其燃烧性能进行考察。结果表明:通过球磨法可以将lih嵌入Al粉内部,lih含量是影响其微观形貌及粒径、粒径分布的关键因素,lih的加入使得燃料质量热值增加,同时随着lih含量的增加Al‐lih复合燃料燃烧火焰强度增强,点火延迟时间大幅降低。其中由于Al‐lih复合燃料在高温下发生了微爆现象,Al‐3lih和Al‐10lih复合燃料在一次氧化后分别实现第二次氧化。

关键词：铝粉氢化锂推进剂燃烧性能

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Ubiquitin phosphorylation in Parkinson’s disease:implications for pathogenesis and treatment

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Translational Neurodegeneration 2016年第1期5卷 1-8页

作者： lih-Shen Chin Lian Li Department of Pharmacology and Center for Neurodegenerative Disease Emory University School of MedicineAtlantaGA 30322USA

Parkinson’s disease(PD)is the most common neurodegenerative movement disorder,characterized primarily by the loss of dopaminergic neurons in substantia *** pathogenic mechanisms of PD remain unclear,and no effective therapy currently exists to stop neurodegeneration in this debilitating *** identification of mutations in mitochondrial serine/threonine kinase PiNK1 or E3 ubiquitin-protein ligase parkin as the cause of autosomal recessive PD opens up new avenues for uncovering neuroprotective pathways and PD pathogenic *** studies reveal that PiNK1 translocates to the outer mitochondrial membrane in response to mitochondrial depolarization and phosphorylates ubiquitin at the residue *** phosphorylated ubiquitin serves as a signal for activating parkin and recruiting autophagy receptors to promote clearance of damaged mitochondria via *** evidence has begun to indicate a link between impaired ubiquitin phosphorylation-dependent mitophagy and PD pathogenesis and supports the potential of Ser65-phosphorylated ubiquitin as a biomarker for *** new mechanistic insights and phenotypic screens have identified multiple potential therapeutic targets for PD drug *** review highlights recent advances in understanding ubiquitin phosphorylation in mitochondrial quality control and PD pathogenesis and discusses how these findings can be translated into novel approaches for PD diagnostic and therapeutic development.

关键词： Mitophagy Parkinson’s disease PiNK1 Parkin Ubiquitin-protein ligase Ubiquitin phosphorylation Mitochondrial quality control Mitophagy

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Hypertonia-linked protein Trakl functions with mitofusins to promote mitochondrial tethering and fusion

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Protein & Cell 2018年第8期9卷 693-716页

作者： Crystal A. Lee lih-Shen Chin Lian Li Department of Pharmacology Emory University School of Medicine Atlanta GA 30322 USA Cell Biology Section Neurogenetics Branch National Institute of Neurological Disorders and Stroke National Institutes of Health Bethesda MD 20892 USA

Hypertonia is a neurological dysfunction associated with a number of central nervous system disorders, including cerebral palsy, Parkinson＇s disease, dystonia, and epilepsy. Genetic studies have identified a homozygous truncation mutation in Trakl that causes hypertonia in mice. Moreover, elevated Trakl protein expression is associated with several types of cancers and variants in Trakl are linked to childhood absence epilepsy in humans. Despite the importance of Trakl in health and disease, the mechanisms of Trakl action remain unclear and the pathogenic effects of Trakl mutation are unknown. Here we report that Trakl has a crucial function in regulation of mitochondrial fusion. Depletion of Trakl inhibits mitochondrial fusion, result- ing in mitochondrial fragmentation, whereas overex- pression of Trakl elongates and enlarges mitochondria. Our analyses revealed that Trakl interacts and colocal- izes with mitofusins on the outer mitochondrial mem- brane and functions with mitofusins to promote mitochondrial tethering and fusion. Furthermore, Trakl is required for stress-induced mitochondrial hyperfu- sion and pro-survival response. We found that hyper- tonia-associated mutation impairs Trakl mitochondrial localization and its ability to facilitate mitochondrial tethering and fusion. Our findings uncover a novel function of Trakl as a regulator of mitochondrial fusion and provide evidence linking dysregulated mitochon- drial dynamics to hypertonia pathogenesis.

关键词： mitochondria mitochondrial fusion mitochondrial tethering mitofusin hypertonia

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A combinatorial approach of developing alloy thin films using co-sputtering technique for displays

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Science China(Technological Sciences) 2009年第1期52卷 79-87页

作者： Jaydeep SARKAR Tien-Heng HUANG lih-Ping wang Peter H. McDONALD Chi-Fung LO Paul S. GiLMAN Praxair Electronics 560 Route 303 Orangeburg Materials and Chemical Research Laboratories Photovoltaic Technology Center Industrial Technology Research Institute (ITRI)

in this study we have used a combinatorial approach for producing binary and ternary alloy thin film libraries using a lab-scale RF co-sputtering system. initially we used two elemental sputtering targets, i.e. aluminum (Al) target and neodymium (Nd) target, to produce a film library of varying composition and successfully identified a suitable composition range (1.95–2.38 at% Nd) in which resistance to hillock formation and resistivity of the film spots were found to be satisfactory in annealed state (350°C, 30 min). in another case, in order to form ternary alloy composition library we have used two sputtering targets, i.e. an Al-0.5 at% Nd alloy target and an elemental Ni target. Though, co-sputtered Al-0.6 at% Nd-0.9 at% Ni alloy films showed satisfactory resistance to hillock formation and low resistivity after annealing, film deposited from a ternary alloy target with the same composition failed to show satisfactory resistance to hillock formation during annealing. in case of Al-0.6 at% Nd-0.9 at% Ni alloy target, 250 nm thick film showed poor resistance to hillock formation than the 500 nm thick film. This clearly showed thickness-dependent hillock performance of Al-0.6 at% Nd-0.9 at% Ni alloy. in this study it was found that, in addition to the process variables, metallurgical microstructure of the alloy sputtering targets had significant effect on the film properties which was not obvious from the results of films deposited using co-sputtering of the individual elemental targets.

关键词： co-sputtering thin films aluminum alloys displays

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