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A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis

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Chinese Chemical Letters 1999年第3期10卷 235-238页

作者： Met FANG Li SHENG Hut Wan HAN Xiao YU Rut ZHAO Guo Quan LIU(Laboratory of Analytical Chemistry in life science, institute of Chemistry, Chinese Academy ofsciences，Beijing 100080) Lab. of Analyt. Chem. in life Sci. Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China

A novel capillary electrophoresis method coupled with on-line microdialysis using an attachable electrode has been developed to study the interaction of macromolecule with small molecule. The binding constants of bovine serum album (BSA) with D,L-tryptophan (Trp), sulfamethoxazole (SMZ) with trypsin and chymotrypsin were determined. These values are 2.3 x 10(4)L/mol for BSA-L-Trp; 1.77 x 10(3) L/mol for BSA-D-Trp in pH 7.4, 50 mmol/ L phosphate; 1.4 x 10(4) L/mol for SMZ- trypsin and 6.0 x 10(3) L/mol for SMZ-chymotrypsin in pH 6.5, 25 mmol/L Tris buffer. The proposed method has merits of speed, low sample consumption and readily available to be performed in desired physiological conditions.

关键词： interaction of macromolecule with small molecule binding constant affinity capillary electrophoresis microdialysis membrane

Fluorescence Resonance Energy Transfer Analysis of Bid Activation in Living Cells during Ultraviolet-induced Apoptosis

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Acta Biochimica et Biophysica Sinica 2007年第1期39卷 37-45页

作者： Yinyuan WU Da XING Lei LIU Tongsheng CHEN and Wei R.CHEN MOE Key Laboratory of Laser life science &institute of life science,South China Normal University,Guangzhou 510631,China MOEKeyLaboratoryofLaserlifescience&instituteofLaserlifescience SouthChinaNormalUniversityGuangzhou510631China

Ultraviolet (UV) irradiation is a DNA-damaging agent that triggers apoptosis through both themembrane death receptor and mitochondrial apoptotic signaling ***,a pro-apoptotic Bcl-2family member,is important in most cell types to apoptosis in response to DNA *** this study,arecombinant plasmid,YFP-Bid-CFP,comprised of yellow and cyan fluorescent protein and a full length Bid,was used as a fluorescence resonance energy transfer analysis (FRET) *** the FRET techniquebased on YFP-Bid-CFP,we found that Bid activation was initiated at 9±1 h after UV irradiation,and theaverage duration of the activation was 75±10 *** activation coincided with a collapse of the mitochondrialmembrane potential with an average duration of 50±10 min. When cells were pretreated with Z-IETD-fmk(caspase-8 specific inhibitor) the process of Bid activation was completely inhibited,but the apoptosis wasonly partially affected.Z-DEVD-fmk (caspase-3 inhibitor) and Z-FA-fmk (non asp specific inhibitor) didnot block Bid ***,the endogenous Bid activation with or without Z-IETD-fmk in responseto UV irradiation was confirmed by Western *** summary, using the FRET technique,we observedthe dynamics of Bid activation during UV-induced apoptosis and found that it was a caspase-8 dependentevent.

关键词： ultraviolet irradiation Bid activation apoptosis caspase-8 fluorescence resonance energy transfer analysis

电子束激励的稀有气体碱金属离子准分子(XeRb)^+和(KrRb)^+的真空紫外发射

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中国激光 1997年第5期24卷 401-405页

作者：邢达华南师范大学激光与生命科学研究所

由相对论电子束激发稀有气体和碱金属Ｒｂ的混合蒸气，观察到来自（ＸｅＲｂ）＋和（ＫｒＲｂ）＋离子准分子的两个真空紫外发射带，其波长分别在１６４ｎｍ和１３３ｎｍ。

关键词：真空紫外发射离子准分子电子束激发激光物理

Disassembly intermediates of RbsD protein remain oligomeric despite the loss of an intact secondary structure

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science China(life sciences) 2009年第11期52卷 997-1002页

作者： FENG YongJun1, ZHANG Meng2, HU MingXi1, ZHENG Jie1, JIAO WangWang2 & CHANG ZengYi2 1 School of life science, Beijing institute of Technology, Beijing 100081, China 2 School of life sciences, National Laboratory of Protein Engineering and Plant Genetic Engineering, Center for Protein science, Peking University, Beijing 100871, China School of life science Beijing Institute of Technology Beijing Chin

Many proteins exist as homo-oligomers in living organisms wherein the change of oligomeric status apparently serves as an effective means for modulating their biological activities. We have previously reported that the homo-decameric RbsD from Escherichia coli undergoes stepwise disassembly and non-stepwise reassembly. Here the structural status of the urea-induced RbsD disassembly intermediates was examined, mainly using urea-containing polyacrylamide gel electrophoresis and chemical cross-linking. Such intermediates were found to remain oligomeric while losing their intact secondary structures. Such disassembly intermediates were able to effectively refold when the concentration of the urea denaturant was reduced to a lower level, or to refold/reassemble into the native decamers when urea was completely removed, as detected by non-denaturing polyacrylamide gel electrophoresis. These novel observations strongly suggest that the assembly of oligomeric proteins may occur before the completion of subunit folding.

关键词： RbsD oligomeric protein oligomeric intermediate refolding reassembly

Acetazolamide inhibits aquaporin-1 protein expression and angiogenesis

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Acta Pharmacologica Sinica 2004年第6期25卷 110-114页

作者： Yang XIANG2, Bing MA, Tao LI, Jun-wei GAO, He-ming YU3, Xue-jun LI4 Departmentof Pharmacology, School of Basic Medical sciences, Peking University, Beijing 100083 2life science institute, Nanjing University, Nanjing 210093 3National Research institute for Family Planning, Beijing 100081,China Department of Pharmacology School of Basic Medical Sciences Peking University Beijing 100083 China

AIM: To study effects of acetazolamide on aquaporin-1 (AQP1) protein expression and angiogenesis. METHODS: Establishing Lewis-lung-carcinoma model, the localization of AQP1 in tumor tissues was investigated by immuno- histochemical methods; The biological activity of acetazolamide was detected by endothelial cells proliferation test (MTT) assay and chorioallantoic membrane (CAM) vascular inhibition test. RESULTS: Immunohistochemical localization of AQP1in mice tumor was labeled in capillaries, post capillary venules endothelial cells. After being treated with acetazolamide, the number of capillaries and post capillary venules was significantly decreased in tumor tissue. Acetazolamide showed significant inhibitory effect on angiogenesis in CAMand endothelial cell proliferation. CONCLUSION: Acetazolamide might be identified and developed as one of potential lead compounds for a new therapeutic intervention in inhibiting cancer angiogenesis.

关键词： neoplasms acetazolamide angiogenesis inhibitors CHIP28

Revenue Sharing in Dairy Industry Supply Chain-A Case Study of Hohhot,China

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Journal of Integrative Agriculture 2013年第12期12卷 2300-2309页

作者： QIAN Gui-xia ZHANG Yi-pin WU Jian-guo PAN Yue-hong School of Economics & Management Inner Mongolia University Sino-US Center for Conservation Energy and Sustainability Science in Inner Mongolia School of life science & Global institute of Sustainability Arizona State University Agricultural Information institute Chinese Academy of Agricultural Sciences

Dairy industry has become an increasingly important enterprise in China as people＇s dietary preferences and composition have changed dramatically with rapid economic development in the past several decades.A number of problems,however,exist in China＇s relatively young dairy industry,including the imbalanced allocation of profits throughout the dairy supply *** of the root causes of the melamine infant powered milk scandal in 2008 was the unfair profit allocation mechanism in dairy supply *** revenue sharing contract approach has proven to be effective in generating market shares and total *** this study,we apply the three-stage revenue sharing contract model of Giannoccaro and Pontrandolfo（2004） in an analysis of dairy supply chain to explore its problems in profit allocation and possible solutions to *** analysis was conducted by a case study of Hohhot,often called as ＂milk capital of China＂.Our results show that the current profit distribution in the dairy supply chain is not balanced：the supermarket＇s profitfarmer＇s profitmanufacturer＇s *** the revenue sharing contract setting,the dairy industry＇s total profit increased by 12.49%.By exploring different parameters in the revenue sharing contract model,we have found that a win-win situation can be created among all the members of the supply *** dairy supply chain,the ratio of the revenue reserved for the supermarket itself is equal or greater than 47% and the ratio of the revenue reserved for the manufacturer itself is between 46.4 and 50.2%.The values of the parameters that generate a sustainable or win-win situation are related to the bargaining position in the dairy supply *** revenue sharing contract has proven to be effective and desirable by all the dairy chain partners in dairy supply *** results of this study provide relevant information for improving the dairy supply chain structure and the revenue sharing contract model can be applied to other industries,s

关键词： Hohhot dairy industry revenue sharing contract win-win

A novel biosensor for rapid identification of high temperature resistant species

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A novel biosensor for rapid identification of high temperatu...

Noninvasive mapping of Subcutaneous Vasculature with High Resolution Photoacoustic Imaging

2007亚洲光电子会议先进的传感系统与应用分会场

作者： Lingrui Zhang Da Xing and Xiaoming Zhou MOE Key Laboratory of Laser life science & institute of Laser life science South China Normal University Guangzhou 510631 China

In this paper, a novel biosensor technique for identification of high temperature resistant species based on quantitative measurement of delayed fluorescence （DF） is described. The biosensor, which uses light-emitting diode lattice as excitation light source, is portable and can detect DF emission from plants in vivo. Compared with its primary version in our previous report, the biosensor presented here can better control environmental factors. Moreover, the improved biosensor can automatically complete the measurements of light response curves of DF intensity in a programmed mode. The testing of the improved biosensor has been made in two maize species （Zea May L.） after high temperature treatment. Contrast evaluations of the effects of heat stress on seedlings photosynthesis were made from measurements of net photosynthesis rate （Pn） based on consumption of CO2. Current testing has demonstrated that the DF intensity well correlates with Pn in each plant species after heat stress. We thus conclude that the DF technique is a breakthrough to traditional strategy of identifying the differences in heat tolerance based on gas exchange, and can provide a reliable approach for rapid and non-invasive determination of the effects of heat stress on photosynthesis and identification of high temperature resistant species.

关键词： Biosensor delayed fluorescence net photosynthesis rate heat stress heat tolerance

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Noninvasive mapping of Subcutaneous Vasculature with High Re...

Lysosomal Photodamage Induces Cell Death via Mitochondrial Apoptotic Pathway

2007亚洲光电子会议保健与生物医学光学分会场

作者： Yeqi Lao Da Xing Sihua Yang MOE Key laboratory of Laser life science & institute of Laser life science South China Normal University Guangzhou 510631 CHINA

As a novel hybrid imaging modality, photoacoustic （PA） imaging combines the merits of high optical contrast, good ultrasonic resolution and sufficient imaging depth, which may be of great benefit to noninvasively detect and monitor the pathological changes of subcutaneous vasculature, e.g., congenital vascular tumor and vascular malformation. In this paper, we apply a set of photoacoustic imaging system to image a sample of subcutaneous blood vessels, which is used to simulate the location of human’s subcutaneous vasculature. Furthermore, an image of subcutaneous vasculature of the abdomen in a mouse is acquired in vivo. Laser pulses at a wavelength of 532 nm from a Q-switched Nd:YAG laser are employed as light source to generate PA signals in the experiments, because the optical absorption of whole blood is much stronger than that of other tissues at this wavelength. A needle polyvinylidene fluoride （PVDF） hydrophone with a diameter of 1mm is used to capture PA signals through a circular scan. The experimental results show that detailed structural information of subcutaneous vasculature, such as the shape and position of the blood vessels and the vessel branching, is clearly revealed by the PA imaging system. The spatial resolution of the PA imaging system reaches 80μm. Moreover, the reconstructed image of a mouse’s abdomen in vivo demonstrates that this technique is suitable for noninvasive subcutaneous vasculature imaging. All of the results prove that the PA imaging can be used as a helpful tool for monitoring the pathological changes of subcutaneous vasculature.

关键词： photoacoustic imaging subcutaneous vasculature high resolution

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Lysosomal Photodamage Induces Cell Death via Mitochondrial A...