A Novel Method for Studying the interaction of Macromolecule with Small Molecule by Means of Affinity Capillary Electrophoresis

作     者：Met FANG Li SHENG Hut Wan HAN Xiao YU Rut ZHAO Guo Quan LIU(Laboratory of Analytical Chemistry in Life Science, Institute of Chemistry, Chinese Academy ofSciences，Beijing 100080) 

作者机构：Lab. of Analyt. Chem. in Life Sci. Institute of Chemistry Chinese Academy of Sciences Beijing 100080 China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：1999年第10卷第3期

页      面：235-238页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 

主　　题：interaction of macromolecule with small molecule binding constant affinity capillary electrophoresis microdialysis membrane 

摘      要：A novel capillary electrophoresis method coupled with on-line microdialysis using an attachable electrode has been developed to study the interaction of macromolecule with small molecule. The binding constants of bovine serum album (BSA) with D,L-tryptophan (Trp), sulfamethoxazole (SMZ) with trypsin and chymotrypsin were determined. These values are 2.3 x 10(4)L/mol for BSA-L-Trp; 1.77 x 10(3) L/mol for BSA-D-Trp in pH 7.4, 50 mmol/ L phosphate; 1.4 x 10(4) L/mol for SMZ- trypsin and 6.0 x 10(3) L/mol for SMZ-chymotrypsin in pH 6.5, 25 mmol/L Tris buffer. The proposed method has merits of speed, low sample consumption and readily available to be performed in desired physiological conditions.