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BE-PIGS: a base-editing tool with deaminases inlaid into Cas9 PI domain significantly expanded the editing scope

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Signal Transduction and Targeted Therapy 2019年第1期4卷 327-329页

作者： Yanhong Wang lifang zhou Nan Liu Shaohua Yao State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan University610041 Chengdu People’s Republic of China

Dear Editor,The discovery of base-editing tools sheds new light on both basic biomedical research and clinical therapeutics.1,2 Although showing profound performance in a wide range of applications,current base editors have their *** particular,the narrow editing windows of these base editors have significantly limited their targetable *** current base editors,the most frequent way to tether deaminase is to fuse it to the N-terminus of the Cas9 protein through flexible peptide *** editors derived from Streptococcus pyogenes Cas9(SpyCas9)or its variants can efficiently edit desired bases in an~5 nt wide window within the detached,non-target strand(NTS).Maximum editing typically occurs around positions 5–7,counting from the 5′end of the protospacer,which is about 15 nt away from the protospacer adjacent motif(PAM).

关键词： base expanded editing

Influence of Bias on the Properties of Carbon Nitride Films Prepared by Vacuum Cathodic Arc Method

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Journal of Materials Science & Technology 2004年第6期20卷 735-738页

作者： Zhimin zhou, lifang XIA and Mingren SUNSchool of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China Sch. of Mat. Sci. and Eng. Harbin Inst. of Technol. Harbin 150001 China

Carbon nitride films have been synthesized in a wide range of biases from 0 to -900 V by vacuum cathodic arc method. The N content was about 12.0-22.0 at. pct. Upon increasing the biases from 0 to -100 V, the N content increased from 15.0 to 22.0 at. pct which could be attributed to the knot-on effect. While the further increasing biases led to the gradual falling of the N content to 12.0 at. pct at -900 V due to the enhancement of the sputtering effect. Below -200 V, with the increasing biases the sp2C fraction in the films decreased, as a result of vvhich the I(D)/I(G) fell in the Raman spectra and the sp peaks also showed the decreasing tendency relative to the s peaks in the VBXPS (valence band X-ray photoelectron spectroscopy). While above -200 V, the sp2C fraction increased and the films became graphitinized gradually, accompanying which the I(D)/I(G) rose from -200 V to -300 V and the Raman spectra even shovved the graphite characteristic above -300 V and the sp peaks rose again relative to the s peak. The carbon nitride films mainly consist of three types of bonding: CC, sp2CN and sp3CN bonds. In the first stage the sp3CN relative ratio rises and falls in the second stage, which corresponded well with the variation of the sp2C in the films. The subpiantation mechanism resulting from the effect of ion energy played an important role in deciding'the variation of the microstructure of the carbon nitride films.

关键词： Carbon nitride Vacuum cathodic arc Raman spectroscopy X-ray photoelectron spectroscopy

Associations of Occupational Stress and Coping Styles with Well-Being Among Couriers-Three Cities,Zhejiang Province,China,2021

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China CDC weekly 2023年第20期5卷 446-451,I0002页

作者： Panqi Xue Yixin Zhang Fang Wei lifang zhou Xinglin Fang Yong Hu Yu Hong Shuang Li Xiaoming Lou Hua Zou Institute of Occupational Health and Radiation Protection Zhejiang Provincial Center for Disease Control and PreventionHangzhou CityZhejiang ProvinceChina Department of Public Health Hangzhou Normal UniversityHangzhou CityZhejiang ProvinceChina National Institute of Occupational Health and Poison Control Chinese Center for Disease Control and PreventionBeijingChina

Summary What is already known about this topic?Prior research has primarily concentrated on occupational health concerns,including injuries and heatstroke,among ***,there has been a scarcity of emphasis on mental health aspects,with existing studies predominantly addressing the risk factors associated with occupational *** is added by this report?The present study demonstrated a significant association between occupational stress and well-being among couriers,with positive coping strategies acting as a mediating ***,the results indicate that implementing a positive coping style may mitigate the impact of occupational stress on *** are the implications for public health practice?Future public policy initiatives should focus on promoting the well-being of couriers by fostering improvements in the workplace environment,reevaluating the organization of work,and delivering support to couriers in managing occupational stress.

关键词： injuries policy primarily

WT-PE:Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing

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Signal Transduction and Targeted Therapy 2022年第5期7卷 1756-1763页

作者： Rui Tao Yanhong Wang Yun Hu Yaoge Jiao lifang zhou Lurong Jiang Li Li Xingyu He Min Li Yamei Yu Qiang Chen Shaohua Yao From laboratory of Biotherapy National Key Laboratory of BiotherapyCancer CenterWest China HospitalSichuan universityRenmin Nanlu 17Chengdu 610041 SichuanChina

Large scale genomic aberrations including duplication,deletion,translocation,and other structural changes are the cause of a subtype of hereditary genetic disorders and contribute to onset or progress of *** current prime editor,PE2,consisting of Cas9-nickase and reverse transcriptase enables efficient editing of genomic deletion and insertion,however,at small ***,we designed a novel prime editor by fusing reverse transcriptase(RT)to nuclease wild-type Cas9(WT-PE)to edit large genomic fragment.

关键词： Cas9 nuclease editor

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内蒙古晋语形容词“牛”[■ni■]

方言 2016年第3期38卷 375-379页

作者：周利芳陕西师范大学国际汉学院

内蒙古晋语中的形容词"牛"表示神气、得意等意思的时候读[■ni■],和效摄今读同音,和流摄三等其他字的今读不同音。

关键词：内蒙古晋语形容词 “牛[cnio]”

Model switching method of multi-hierarchical model predictive control system

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化工学报 2012年第4期63卷 1132-1139页

作者：刘琳琳周立芳浙江大学控制科学与工程学系浙江杭州310027

实际的工业过程对象,大部分都呈现出很强的非线性特性,其控制过程十分复杂。虽然近年来,对非线性技术的研究已经取得了很多的成果。但是非线性系统精确建模困难~([1])、非线性微分方程求解烦琐等问题仍然阻碍着非线性控制器的发展。针... 详细信息

实际的工业过程对象,大部分都呈现出很强的非线性特性,其控制过程十分复杂。虽然近年来,对非线性技术的研究已经取得了很多的成果。但是非线性系统精确建模困难~([1])、非线性微分方程求解烦琐等问题仍然阻碍着非线性控制器的发展。针对这些问题,很多学者根据处理复杂问题使用的分解-合成策略,提出了多模型方法~([2]。多模型方法的基本思想是把整个操作区间用某种划分方法分成若干个子区间,每个子区间上建立对应的子模型,在系统运行时,使用子模型代替全局模型。

关键词：多层次结构模型 k均值聚类算法模型预测控制多层次结构模型切换 pH中和过程

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基于聚类多模型建模的多模态预测控制

化工学报 2008年第10期59卷 2546-2552页

作者：周立芳张赫男浙江大学信息学院控制系工业控制研究所先进控制研究所浙江杭州310027

多模型预测控制(MMPC)是解决非线性控制问题的重要手段,本文针对多模态控制器设计中模态匹配准则的选取问题,利用当前样本状态与各聚类建模子空间距离差异,提出了一种基于距离匹配的多模型控制器加权算法。然后,基于模态融合思想,提出... 详细信息

多模型预测控制(MMPC)是解决非线性控制问题的重要手段,本文针对多模态控制器设计中模态匹配准则的选取问题,利用当前样本状态与各聚类建模子空间距离差异,提出了一种基于距离匹配的多模型控制器加权算法。然后,基于模态融合思想,提出了模态加权构建实时预测模型的控制策略。通过对pH中和过程进行仿真,结果表明:两种方法都提高了非线性系统的暂态响应,跟踪特性优良,体现了它们对非线性系统大范围控制的有效性。

关键词：多模型切换策略多模型预测控制多模态控制器设计模型融合

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热耦合空分流程变负荷特性分析与模拟方法

化工学报 2011年第8期62卷 2232-2237页

作者：祝铃钰周立芳钱积新浙江工业大学化学工程与材料学院浙江杭州310014 浙江大学控制系浙江杭州310027

针对管网升压事件讨论低温空分三塔精馏热耦合流程的变负荷操作模拟问题。分析认为,此大规模变负荷问题的求解难点在于耦合流程操作变量的微小变化引起了精馏塔浓度波形的大幅度移动,某些变量在微小步长上产生数量级的变化,基于牛顿法... 详细信息

针对管网升压事件讨论低温空分三塔精馏热耦合流程的变负荷操作模拟问题。分析认为,此大规模变负荷问题的求解难点在于耦合流程操作变量的微小变化引起了精馏塔浓度波形的大幅度移动,某些变量在微小步长上产生数量级的变化,基于牛顿法及其改进算法的数值计算方法在梯度计算中难以获得正确的下降方向,导致收敛困难。利用工况变量构造同伦参数并采用回溯同伦法(HBM)进行自动搜索,可以有效解决考虑耦合换热器换热面积计算的变负荷问题,能够描述因粗氩塔顶换热温差减小引起的'氮塞'现象,对过程控制更有指导意义。

关键词：耦合回溯同伦空分流程模拟

Forelli-Rudin型定理和一类与超几何函数相关的积分算子

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中国科学：数学 2019年第5期49卷 765-780页

作者：周立芳卢金湖州师范学院数学系湖州313000

借助超几何函数与Schur检验等理论知识,本文讨论了一类与超几何函数相关的积分算子在L^p(0, 1)上的有界性与精确范数.主要结果的建立不仅为全纯Forelli-Rudin型定理与调和Forelli-Rudin型定理搭建了桥梁,而且也深化了对Bergman投影和Ber... 详细信息

借助超几何函数与Schur检验等理论知识,本文讨论了一类与超几何函数相关的积分算子在L^p(0, 1)上的有界性与精确范数.主要结果的建立不仅为全纯Forelli-Rudin型定理与调和Forelli-Rudin型定理搭建了桥梁,而且也深化了对Bergman投影和Berezin变换的认识.

关键词：超几何函数 Bergman投影 Berezin变换精确范数