WT-PE:Prime editing with nuclease wild-type Cas9 enables versatile large-scale genome editing

作     者：Rui Tao Yanhong Wang Yun Hu Yaoge Jiao Lifang Zhou Lurong Jiang Li Li Xingyu He Min Li Yamei Yu Qiang Chen Shaohua Yao 

作者机构：From laboratory of BiotherapyNational Key Laboratory of BiotherapyCancer CenterWest China HospitalSichuan universityRenmin Nanlu 17Chengdu 610041 SichuanChina 

出 版 物：《Signal Transduction and Targeted Therapy》 (信号转导与靶向治疗（英文）)

年 卷 期：2022年第7卷第5期

页      面：1756-1763页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：We thank Yang Yang for providing python script for HTS data analysis.This work was supported by National Natural Science Foundation of China.No.U19A2002 No.81771220 and No.81974238 

主　　题：Cas9 nuclease editor 

摘      要：Large scale genomic aberrations including duplication,deletion,translocation,and other structural changes are the cause of a subtype of hereditary genetic disorders and contribute to onset or progress of *** current prime editor,PE2,consisting of Cas9-nickase and reverse transcriptase enables efficient editing of genomic deletion and insertion,however,at small ***,we designed a novel prime editor by fusing reverse transcriptase(RT)to nuclease wild-type Cas9(WT-PE)to edit large genomic fragment.