检索结果-南通市图书馆

Molecular regulation mechanism of intestinal stem cells in mucosal injury and repair in ulcerative colitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2023年第16期29卷 2380-2396页

作者： lie zheng Sheng-Lei Duan Department of Gastroenterology Shaanxi Hospital of Traditional Chinese MedicineXi’an 730000Shaanxi ProvinceChina

Ulcerative colitis(UC)is a chronic nonspecific inflammatory disease with complex causes.The main pathological changes were intestinal mucosal injury.Leucinerich repeat-containing G protein coupled receptor 5(LGR5)-labeled small intestine stem cells(ISCs)were located at the bottom of the small intestine recess and inlaid among Paneth cells.LGR5+small ISCs are active proliferative adult stem cells,and their self-renewal,proliferation and differentiation disorders are closely related to the occurrence of intestinal inflammatory diseases.The Notch signaling pathway and Wnt/β-catenin signaling pathway are important regulators of LGR5-positive ISCs and together maintain the function of LGR5-positive ISCs.More importantly,the surviving stem cells after intestinal mucosal injury accelerate division,restore the number of stem cells,multiply and differentiate into mature intestinal epithelial cells,and repair the damaged intestinal mucosa.Therefore,in-depth study of multiple pathways and transplantation of LGR5-positive ISCs may become a new target for the treatment of UC.

关键词： Molecular regulation Mucosal injury Regeneration Ulcerative colitis

Research progress on the relationship between Paneth cellssusceptibility genes,intestinal microecology and inflammatory bowel disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2023年第34期11卷 8111-8125页

作者： Qi-Ming Zhou lie zheng Department of Nephrology Lanxi Hospital of Traditional Chinese MedicineLanxi 321100Zhejiang ProvinceChina Department of Gastroenterology Shaanxi Hospital of Traditional Chinese MedicineXi’an 710003Shaanxi ProvinceChina

Inflammatory bowel disease(IBD)is a disorder of the immune system and intestinal microecosystem caused by environmental factors in genetically susceptible people.Paneth cells(PCs)play a central role in IBD pathogenesis,especially in Crohn's disease development,and their morphology,number and function are regulated by susceptibility genes.In the intestine,PCs participate in the formation of the stem cell microenvironment by secreting antibacterial particles and play a role in helping maintain the intestinal microecology and intestinal mucosal homeostasis.Moreover,PC proliferation and maturation depend on symbiotic flora in the intestine.This paper describes the interactions among susceptibility genes,PCs and intestinal microecology and their effects on IBD occurrence and development.

关键词： Susceptibility gene Paneth cells Intestinal microecology Inflammatory bowel disease

Role of metabolites derived from gut microbiota in inflammatory bowel disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第9期10卷 2660-2677页

作者： lie zheng Xin-Li Wen Sheng-Lei Duan Department of Gastroenterology Shaanxi Hospital of Traditional Chinese MedicineXi’an 710003Shaanxi ProvinceChina

Over the past two decades,it is improved gut microbiota plays an important role in the health and disease pathogenesis.Metabolites,small molecules produced as intermediate or end products of microbial metabolism,is considered as one of the major interaction way for gut microbiota with the host.Bacterial metabolisms of dietary substrates,modification of host molecules or bacteria are the major source of metabolites.Signals from microbial metabolites affect immune maturation and homeostasis,host energy metabolism as well as mucosal integrity maintenance.Based on many researches,the composition and function of the microbiota can be changed,which is also seen in the metabolite profiles of patients with inflammatory bowel disease(IBD).Additionally,some specific classes of metabolites also can trigger IBD.In this paper,definition of the key classes of microbialderived metabolites which are changed in IBD,description of the pathophysiological basis of association and identification of the precision therapeutic modulation in the future are the major contents.

关键词： Inflammatory bowel disease Microbial metabolites Short chain fatty acids Bile acids Tryptophan

Fecal microbiota transplantation in the metabolic diseases:Current status and perspectives

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2022年第23期28卷 2546-2560页

作者： lie zheng Yong-Yi Ji Xin-Li Wen Sheng-Lei Duan Department of Gastroenterology Shaanxi Hospital of Traditional Chinese MedicineXi’an 710003Shaanxi ProvinceChina Department of Neurology Xi’an Hospital of Traditional Chinese MedicineXi’an 710021Shaanxi ProvinceChina

With the development of microbiology and metabolomics,the relationship between the intestinal microbiome and intestinal diseases has been revealed.Fecal microbiota transplantation(FMT),as a new treatment method,can affect the course of many chronic diseases such as metabolic syndrome,malignant tumor,autoimmune disease and nervous system disease.Although the mechanism of action of FMT is now well understood,there is some controversy in metabolic diseases,so its clinical application may be limited.Microflora transplantation is recommended by clinical medical guidelines and consensus for the treatment of recurrent or refractory Clostridium difficile infection,and has been gradually promoted for the treatment of other intestinal and extraintestinal diseases.However,the initial results are varied,suggesting that the heterogeneity of the donor stools may affect the efficacy of FMT.The success of FMT depends on the microbial diversity and composition of donor feces.Therefore,clinical trials may fail due to the selection of ineffective donors,and not to faulty indication selection for FMT.A new understanding is that FMT not only improves insulin sensitivity,but may also alter the natural course of type 1 diabetes by modulating autoimmunity.In this review,we focus on the main mechanisms and deficiencies of FMT,and explore the optimal design of FMT research,especially in the field of cardiometabolic diseases.

关键词： Fecal microbiota transplantation Metabolic diseases Inflammatory bowel disease Type 1 diabetes Metabolic syndrome

Network pharmacology and molecular docking reveal zedoary turmeric-trisomes in Inflammatory bowel disease with intestinal fibrosis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第22期10卷 7674-7685页

作者： lie zheng Yong-Yi Ji Yan-Cheng Dai Xin-Li Wen Shi-Cheng Wu Department of Gastroenterology Shaanxi Hospital of Traditional Chinese MedicineShaanxi Provincial Hospital of Traditional Chinese MedicineXi’an 730000Shaanxi ProvinceChina Department of Neurology Xi’an Hospital of Traditional Chinese MedicineXi’an 710021Shaanxi ProvinceChina Department of Gastroenterology Shanghai Traditional Chinese Medicine Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai 200082China Department of Gastroenterology Shaanxi Hospital of Traditional Chinese MedicineXi’an 710003Shaanxi ProvinceChina Department of Proctology Gansu Academy of Traditional Chinese MedicineGanSu Hospital of Traditional Chinese MedicineLanzhou 730050Gansu ProvinceChina

BACKGROUND Inflammatory bowel disease(IBD) is a complex chronic IBD that is closely associated with risk factors such as environment, diet, medications and lifestyle that may influence the host microbiome or immune response to antigens. At present, with the increasing incidence of IBD worldwide, it is of great significance to further study the pathogenesis of IBD and seek new therapeutic targets. Traditional Chinese medicine(TCM) treatment of diseases is characterized by multiple approaches and multiple targets and has a long history of clinical application in China. The mechanism underlying the effect of zedoary turmerictrisomes on inducing mucosal healing in IBD is not clear.AIM To explore the effective components and potential mechanism of zedoary turmeric-trisomes in the treatment of IBD with intestinal fibrosis using network pharmacology and molecular docking techniques.METHODS The chemical constituents and targets of Rhizoma zedoary and Rhizoma sanarum were screened using the TCMSP database. The GeneCards database was searched to identify targets associated with intestinal fibrosis in IBD. The intersection of chemical component targets and disease targets was obtained using the Venny 2.1 online analysis platform, and the common targets were imported into the STRING 11.0 database to construct a protein interaction regulatory network. A “zedoary turmeric-trisomes-chemical composition-target-disease” network diagram was subsequently constructed using Cytoscape 3.7.2 software, and the topological properties of the network were analyzed using the “Network Analysis” plug-in. Gene Ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analyses of the common targets were performed using the DAVID 6.8 database to elucidate the mechanism of zedoary turmeric-trisomes in the treatment of IBD. Subsequently, molecular docking of the compounds and targets with the highest intermediate values in the “zedoary turmeric-trisomes-chemical composition-target-disease” network was performed using Sybyl-x 2.1.1

关键词： Network pharmacology Molecular docking Zedoary turmeric trisomes Inflammatory bowel disease Intestinal fibrosis

Role of adherent invasive Escherichia coli in pathogenesis of inflammatory bowel disease

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第32期10卷 11671-11689页

作者： lie zheng Sheng-Lei Duan Yan-Cheng Dai Shi-Cheng Wu Department of Gastroenterology Shaanxi Provincial Hospital of Traditional Chinese MedicineXi’an 322000Shaanxi ProvinceChina Department of Gastroenterology Shanghai Traditional Chinese Medicine Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai 200082China Department of Proctology Gansu Academy of Traditional Chinese MedicineGansu Hospital of Traditional Chinese MedicineLanzhou 730050Gansu ProvinceChina

Gut microbiota imbalances play an important role in inflammatory bowel disease(IBD),but no single pathogenic microorganism critical to IBD that is specific to the IBD terminal ileum mucosa or can invade intestinal epithelial cells has been found.Invasive Escherichia coli(E.coli)adhesion to macrophages is considered to be closely related to the pathogenesis of inflammatory bowel disease.Further study of the specific biological characteristics of adherent invasive E.coli(AIEC)may contribute to a further understanding of IBD pathogenesis.This review explores the relationship between AIEC and the intestinal immune system,discusses the prevalence and relevance of AIEC in Crohn's disease and ulcerative colitis patients,and describes the relationship between AIEC and the disease site,activity,and postoperative recurrence.Finally,we highlight potential therapeutic strategies to attenuate AIEC colonization in the intestinal mucosa,including the use of phage therapy,antibiotics,and anti-adhesion molecules.These strategies may open up new avenues for the prevention and treatment of IBD in the future.

关键词： Adherent invasive Escherichia coli Inflammatory bowel disease Crohn's disease Ulcerative colitis Prevention

Jianpi Qingchang Bushen decoction improves inflammatory response and metabolic bone disorder in inflammatory bowel disease-induced bone loss

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2022年第13期28卷 1315-1328页

作者： Ya-Li Zhang Qian Chen lie zheng Zi-Wei Zhang Yu-Jun Chen Yan-Cheng Dai Zhi-Peng Tang Institute of Digestive Diseases LongHua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Department of Gastroenterology Traditional Chinese Medicine Hospital of Shaanxi ProvinceXi’an 710003Shaanxi ProvinceChina Department of Gastroenterology Shanghai Traditional Chinese Medicine-Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai 200082China

BACKGROUND Bone loss and osteoporosis are commonly described as extra-intestinal manifestations of inflammatory bowel disease(IBD).Jianpi Qingchang Bushen decoction(JQBD)is a prescription used in clinical practice.However,further studies are needed to determine whether JQBD regulates the receptor activator of nuclear factor kappa B(NF-κB)(RANK)/receptor activator of NF-κB ligand(RANKL)/osteoprotegerin(OPG)pathways and could play a role in treating IBD-induced bone loss.AIM To evaluate the therapeutic effect of JQBD in IBD-induced bone loss and explore the underlying mechanisms.METHODS An IBD-induced bone loss model was constructed by feeding 126-to-8-wk-old interleukin-10(IL-10)-knockout mice with piroxicam for 10 d.The mice were randomly divided into model and JQBD groups.We used wild-type mice as a control.The JQBD group was administered the JQBD suspension for 2 wk by gavage,while the control and model groups were given normal saline at the corresponding time points.All mice were killed after the intervention.The effect of JQBD on body weight,disease activity index(DAI),and colon length was analyzed.Histopathological examination,colon ultrastructure observation,and micro-computed tomographic scanning of the lumbar vertebrae were performed.The gene expression of NF-κB,tumor necrosis factor-α(TNF-α),IL-1β,IL-6,and IL-8 in the colon was evaluated by real-time polymerase chain reaction.Colon samples were assessed by Western blot for the expression of RANKL,OPG,RANK,and NF-κB proteins.RESULTS The model group lost body weight,had a shorter colon,and showed a dramatic increase in DAI score,whereas JQBD had protective and therapeutic effects.Treatment with JQBD significantly improved inflammatory cell infiltration and reduced crypt abscess and ulcer formation.Threedimensional imaging of the vertebral centrum in the model group revealed a lower bone mass,loose trabeculae,and“rod-shaped”changes in the structure compared to the control group and JQBD groups.The bone volume/total volume ratio and bone mineral density were

关键词： Inflammatory bowel disease Osteoporosis Jianpi Qingchang Bushen decoction Inflammation Bone metabolism

Gut microbiota and inflammatory bowel disease: The current status and perspectives

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2021年第2期9卷 321-333页

作者： lie zheng Xin-Li Wen Department of Gastroenterology Shaanxi Provincial Hospital of Traditional Chinese MedicineXi’an 730000Shaanxi ProvinceChina

Inflammatory bowel disease(IBD)is a chronic immune-mediated disease that affects the gastrointestinal tract.It is argued that environment,microbiome,and immune-mediated factors interact in a genetically susceptible host to trigger IBD.Recently,there has been increased interest in the development,progression,and treatment of IBD because of our understanding of the microbiome.Researchers have proved that some factors can alter the microbiome and the pathogenesis of IBD.As a result,there has been increasing interest in the application of probiotics,prebiotics,antibiotics,fecal microbiota transplantation,and gene manipulation in treating IBD because of the possible curative effect of microbiome-modulating interventions.In this review,we summarize the findings from human and animal studies and discuss the effect of the gut microbiome in treating patients with IBD.

关键词： Inflammatory bowel disease Microbiome Inflammation Genetics Antibiotics Probiotics

Single-cell RNA-sequencing combined with bulk RNA-sequencing analysis of peripheral blood reveals the characteristics and key immune cell genes of ulcerative colitis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2022年第33期10卷 12116-12135页

作者： Yan-Cheng Dai Dan Qiao Chen-Ye Fang Qiu-Qin Chen Ren-Ye Que Tie-Gang Xiao lie zheng Li-Juan Wang Ya-Li Zhang Department of Gastroenterology Shanghai Traditional Chinese Medicine-Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai 200082China Department of Gastroenterology Traditional Chinese Medicine Hospital of NingboNingbo 315000Zhejiang ProvinceChina Department of Pathology Shanghai Traditional Chinese Medicine-Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai 200082China Department of Gastroenterology Traditional Chinese Medicine Hospital of Xi’an 730000Shaanxi ProvinceChina Experimental Education Center Shanghai University of Traditional Chinese MedicineShanghai 201203China Institute of Digestive Diseases LongHua Hospital Shanghai University of Traditional Chinese MedicineShanghai 200032China

BACKGROUND Ulcerative colitis(UC)is a complicated disease caused by the interaction between genetic and environmental factors that affects mucosal homeostasis and triggers an inappropriate immune response.Single-cell RNA sequencing(scRNA-seq)can be used to rapidly obtain the precise gene expression patterns of thousands of cells in the intestine,analyze the characteristics of cells with the same phenotype,and provide new insights into the growth and development of intestinal organs,the clonal evolution of cells,and immune cell changes.These findings can provide new ideas for the diagnosis and treatment of intestinal diseases.To identify clinical phenotypes and biomarkers that can predict the response of UC patients to specific therapeutic drugs and thus aid the diagnosis and treatment of UC.METHODS Using the Gene Expression Omnibus(GEO)database,we analyzed peripheral blood cell subtypes of patients with UC by scRNA-seq combined with bulk RNA sequencing(RNA-seq)to reveal the core genes of UC.We then combined weighted gene correlation network analysis(WGCNA)and least absolute shrinkage and selection operator(LASSO)analysis to reveal diagnostic markers of UC.RESULTS After processing the scRNA-seq data,we obtained data from approximately 24340 cells and identified 17 cell types.Through intercellular communication analysis,we selected monocyte marker genes as the candidate gene set for the prediction model.Construction of a WGCNA coexpression network identified RhoB,cathepsin D(CTSD)and zyxin(ZYX)as core genes.Immune infiltration analysis showed that these three core genes were strongly correlated with immune cells.Functional enrichment analysis showed that the differentially expressed genes were closely related to immune and inflammatory responses,which are associated with many challenges in the diagnosis and treatment of UC.CONCLUSION Through scRNA-seq analysis,LASSO diagnostic model building and WGCNA,we identified RhoB,CTSD and ZYX as core genes of UC that are closely related to monocyte infiltration that may serv

关键词： Ulcerative colitis Single-cell RNA-seq Bulk RNA-seq Peripheral blood Key genes