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Response of soil nutrients to terracing and environmental factors in the Loess Plateau of China

Geography and Sustainability 2024年第2期5卷 230-240页

作者： Die chen Wei Wei lidin. chen.bojun.ma Hao Li Department of Soil and Water Conservation Changjiang River Scientific Research InstituteWuhan 430010China State Key Laboratory of Urban and Regional Ecology Research Center for Eco-environmental SciencesChinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China Research Center on Mountain Torrent&Geologic Disaster Prevention of Ministry of Water Resources Wuhan 430010China

Terracing is a widely adopted agricultural practice in mountainous regions around the world that aims to conserve soil and water *** nutrients play a crucial role in determining soil quality,particularly in landscapes prone to *** are influenced by factors such as land-use type,slope aspect,and *** this study,we sought to examine the impact of terracing on soil nutrients(soil organic content(SOC),total nitrogen(TN),nitrate-nitrogen(NO_(3)^(-)-N),ammonium nitrogen(NH_(4)^(+)-N),total phosphorus(TP),available phosphorus(AP),total potassium(TK),and available potassium(AK))and how they vary with environmental factors in the Chinese Loess *** the growing season,we collected 540 soil samples from the 0 to 100 cm soil layer across five major land-use types,different slope aspects,and varying ***,a meta-analysis of literature data further corroborated the effective accumulation of soil nutrients through terracing in the Loess *** findings are as follows:(1)Terraced fields,regardless of land-use type,showed a significant improvement in SOC and TN content.(2)Soil nutrient contents within terraced fields were predominantly higher on sunny slopes.(3)Terraces at lower altitudes are characterized by elevated SOC concentrations.(4)A meta-analysis of literature data pertaining to terracing and soil nutrients in this region confirmed the effective accumulation of soil nutri-ents through *** elucidated outcomes of this study offer a profound theoretical underpinning for the accurate planning and management of terraces,the scientific utilization of land resources,and the enhancement of land productivity.

关键词： Terrace Soil nutrients Land-use Slope aspect Altitude Loess Plateau

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CRISPR/Cas9基因编辑技术水稻育种应用的实验教学设计

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生物工程学报 2024年第4期40卷 1237-1250页

作者：刘亚萍马伯军陈析丰浙江师范大学生命科学学院生物学国家级实验教学示范中心浙江金华321004

CRISPR/Cas9基因编辑技术在作物育种中已具有重要的应用价值,理解与掌握该项技术能够为生物学、农学等专业的学生从事相关科研工作打好基础。为了将CRISPR/Cas9技术融入高校的实验教学中,设计开发了一个创新性教学实验“利用CRISPR/Cas... 详细信息

CRISPR/Cas9基因编辑技术在作物育种中已具有重要的应用价值,理解与掌握该项技术能够为生物学、农学等专业的学生从事相关科研工作打好基础。为了将CRISPR/Cas9技术融入高校的实验教学中,设计开发了一个创新性教学实验“利用CRISPR/Cas9技术提高水稻植株的白叶枯病抗性”。该实验有助于学生更好地理解CRISPR/Cas9技术原理,掌握其操作流程和方法,学习应用该技术对水稻进行定向分子育种改良。在拓展学生知识和技能的同时,推进实验教学的改革与创新。

关键词： CRISPR/Cas9 基因编辑技术水稻育种实验教学设计

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植物执行者抗病基因研究进展

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植物学报 2024年第4期59卷 671-680页

作者：何璐梅马伯军陈析丰浙江师范大学生命科学学院金华321004

在与病原菌的长期斗争过程中,植物进化出复杂而精细的免疫防御系统。抗病(resistant,R)基因的克隆和功能研究极大地促进了人们对植物免疫防御系统的理解。执行者(executor,E)基因作为一类新的植物抗病基因,具有独特的抗病特点,同时也是... 详细信息

在与病原菌的长期斗争过程中,植物进化出复杂而精细的免疫防御系统。抗病(resistant,R)基因的克隆和功能研究极大地促进了人们对植物免疫防御系统的理解。执行者(executor,E)基因作为一类新的植物抗病基因,具有独特的抗病特点,同时也是重要的抗病基因资源,因此成为植物免疫领域的研究热点。近年来,E基因的克隆和功能机制研究取得了一系列重要进展,但尚未见相关中文综述。该文全面总结了E基因的蛋白序列特征、与病原菌的互作机制、生物学功能及育种应用,以期为深入理解植物-病原菌互作机制和作物抗病育种提供重要参考。

关键词：植物-病原菌互作执行者基因基因克隆分子机制育种应用

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草莓YABBY基因家族的鉴定及表达分析

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生物工程学报 2024年第1期40卷 104-121页

作者：余婷婷沈淑容许以灵王欣雨余瑶马伯军陈析丰浙江师范大学生命科学学院浙江金华321004

YABBY蛋白是一类调控植物形态建成与器官发育的重要转录因子。为了研究草莓YABBY,通过生物信息学技术鉴定了森林草莓(二倍体)与栽培草莓(八倍体)的YABBY基因家族成员及其序列特征,构建了各成员与植物YABBY的系统发育树与共线性关系图,... 详细信息

YABBY蛋白是一类调控植物形态建成与器官发育的重要转录因子。为了研究草莓YABBY,通过生物信息学技术鉴定了森林草莓(二倍体)与栽培草莓(八倍体)的YABBY基因家族成员及其序列特征,构建了各成员与植物YABBY的系统发育树与共线性关系图,并利用RNA-seq数据与定量逆转录聚合酶链反应(quantitative reverse transcription-polymerase chain reaction,qRT-PCR)技术分析其表达模式,构建绿色荧光蛋白(green fluorescent protein,GFP)融合表达载体,在烟草叶细胞中瞬时表达观察其亚细胞定位。研究结果表明,森林草莓有6个FvYABBY基因,栽培草莓有26个FxaYABBY基因;FvYABBY基因家族成员可归为5个不同的进化分支,与拟南芥AtYABBY基因家族存在5对直系同源。在森林草莓中,所有Fv YABBY在根和花托中基本不表达,FvYABBY1、FvYABBY2、FvYABBY5和FvYABBY6在叶、茎、花和瘦果中高表达;在栽培草莓中,FxaYABBY1、FxaYABBY2、FxaYABBY5和FxaYABBY6在瘦果中表达,所有FxaYABBY在花托中为低表达或不表达。此外,在低温、高盐和干旱等非生物胁迫下,FvYABBY1、FvYABBY3、FvYABBY4和FvYABBY6下调表达,FvYABBY5上调表达,FvYABBY2则先上调后下调表达。在烟草叶细胞中,所有FvYABBY蛋白均定位在细胞核。以上结果为草莓YABBY基因的后续功能研究奠定了基础。

关键词：草莓 YABBY基因家族序列分析表达模式亚细胞定位

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Fine mapping of the rice bacterial blight resistance gene Xa-4 and its co-segregation marker

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Chinese Science Bulletin 2000年第19期45卷 1779-1782页

作者： WANG Wenming ZHOU Yongli JIANG Guanhuai ma bojun chen Xuewei ZHANG Qi ZHU Lihuang ZHAI Wenxue Plant Biotechnology Laboratory Chinese Academy of SciencesBeijing 100101China Crop Institute Chinese Academy of Agricultural SciencesBeijing 100081China

An F2 population developed from the Xa-4 near isogenic lines, IR24 and IRBB4, was used for fine mapping of the rice bacterial blight resistance gene, Xa-4. Some restriction fragment length polymorphism （RFLP） markers on the high-density map constructed by Harushima et al. and the amplified Dma fragments homologous to the conserved domains of plant disease resistance （R） genes were used to construct the genetic linkage map around the gene Xa-4 by scoring susceptible individuals in the population. Xa-4 was mapped between the RFLP marker G181 and the polymerase chain reaction （PCR） marker M55. The R gene homologous fragment marker RS13 was found co-segregating with Xa-4 by analyzing all the plants in the population. This result opened an approach to map-based cloning of this gene, and marker RS13 can be applied to molecular marker-assisted selection of Xa-4 in rice breeding programs.

关键词： rice bacterial blight resistance gene Xa-4 genetic map molecular marker.

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2022年北京市一起产气荚膜梭菌引起的食源性疾病暴发事件病原学分析

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疾病监测 2024年第3期39卷 382-387页

作者：赵凤玲高翔邹林江南罗宇馨黄震甄博珺张萍李颖陈倩马晓晨张晓嫒北京市通州区疾病预防控制中心北京101100 北京市顺义区疾病预防控制中心北京101300 北京市顺义区疾病预防控制中心微生物感染性疾病检测工作站北京101300 北京市疾病预防控制中心北京100013 食物中毒诊断溯源技术北京市重点实验室北京100013

目的对一起北京市某学校由产气荚膜梭菌导致的腹泻暴发事件进行病原学分析,为类似疫情的快速精准分析与处置提供参考。方法采用实时荧光聚合酶链式反应(PCR)方法对样本进行多重病原筛查;根据初筛结果进行常规细菌分离培养、生化鉴定... 详细信息

目的对一起北京市某学校由产气荚膜梭菌导致的腹泻暴发事件进行病原学分析,为类似疫情的快速精准分析与处置提供参考。方法采用实时荧光聚合酶链式反应(PCR)方法对样本进行多重病原筛查;根据初筛结果进行常规细菌分离培养、生化鉴定和质谱分析;分离到的产气荚膜梭菌进行PCR毒力基因检测和脉冲场凝胶电泳分子分型。结果 28份病例肛拭子样本中有26份产气荚膜梭菌核酸初筛阳性,分离菌株26株;13份粪便样本有10份产气荚膜梭菌核酸初筛阳性,4份样本分离到20株产气荚膜梭菌;其他样本未检测到病原;46株菌均携带cpa基因和cpe基因,为携带肠毒素CPE基因的A型产气荚膜梭菌;46株菌中41株菌集中在2个脉冲场凝胶电泳带型。结论本次腹泻暴发事件由产气荚膜梭菌(含肠毒素)导致,分子生物学方法可用于产气荚膜梭菌暴发事件的快速检测。

关键词：产气荚膜梭菌暴发毒力基因脉冲场凝胶电泳

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Fluorinated soft carbon as an ultra-high energy density potassium-ion battery cathode enabled by a ternary phase K_(x)FC

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Journal of Energy Chemistry 2023年第2期77卷 38-44,I0002页

作者： Pengyu chen bojun Wang Zhenrui Wu Xiaobin Niu Chuying Ouyang Hong Li Liping Wang School of materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731SichuanChina School of Engineering The University of British ColumbiaKelowna V1V 1V7Canada C Innovation Laboratory Contemporary Amperex Technology Ltd.(21C LAB)Ningde 352100FujianChina Key Laboratory for Renewable Energy Institute of PhysicsChinese Academy of SciencesBeijing 100190China

Fluorinated carbons(CFx)have been widely applied as lithium primary batteries due to their ultra-high energy *** will be a great promise if CFx can be *** this study,we rationally tune the C-F bond strength for the alkaline intercalated CFx via importing an electronegative weaker element K instead of *** forms a ternary phase K_(x)FC instead of two phases(LiF+C)in lithium-ion ***,we choose a large layer distance and more defects CFx,namely fluorinated soft carbon,to accommodate ***,we enable CFx rechargeable as a potassium-ion battery *** detail fluorinated soft carbon CF_(1.01) presents a reversible specific capacity of 339 ma h g^(-1)(797 Wh kg^(-1))in the 2nd cycle and maintains 330 ma h g^(-1)(726 Wh kg^(-1))in the 15th *** study reveals the importance of tuning chemical bond stability using different alkaline ions to endow batteries with *** work provides good references for focusing on developing reversible electrode materials from popular primary cell configurations.

关键词： Fluorinated carbon High energy density battery Potassium-ion battery Conversion reaction K-free cathode

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Xa7, a new executor R gene that confers durable and broad-spectrum resistance to bacterial blight disease in rice

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Plant Communications 2021年第3期2卷 68-81页

作者： Xifeng chen Pengcheng Liu Le Mei Xiaoling He Long chen Hui Liu Shurong Shen Zhandong Ji Xixi Zheng Yuchen Zhang Zhenyu Gao Dali Zeng Qian Qian bojun ma College of Chemistry and Life Sciences Zhejiang Normal UniversityJinhua 321004China China National Rice Research Institute Chinese Academy of Agricultural SciencesHangzhou 310006China

Bacterial blight(BB)is a globally devastating rice disease caused by Xanthomonas oryzae ***(Xoo).The use of disease resistance(R)genes in rice breeding is an effective and economical strategy for the control of this ***,a majority of R genes lack durable resistance for long-term use under global warming ***,we report the isolation of a novel executor R gene,Xa7,that confers extremely durable,broad-spectrum,and heat-tolerant resistance to *** expression of Xa7 was induced by incompatible Xoo strains that secreted the transcription activator-like effector(TALE)AvrXa7 or PthXo3,which recognized effector binding elements(EBEs)in the Xa7 ***,Xa7 induction was faster and stronger under high *** of Xa7 or co-transformation of Xa7 with avrXa7 triggered a hypersensitive response in *** expression of Xa7 activated a defense response in the absence of Xoo but inhibited the growth of transgenic rice *** addition,analysis of over 3000 rice varieties showed that the Xa7 locuswas found primarily in the indica and aus subgroups.A variation consisting of an 11-bp insertion and a base substitution(G to T)was found in EBEAvrXa7 in the tested varieties,resulting in a loss of Xa7 BB *** a decade of effort,we have identified an important BB resistance gene and characterized its distinctive interaction with Xoo strains;these findings will greatly facilitate research on the molecular mechanism of Xa7-mediated resistance and promote the use of this valuable gene in breeding.

关键词： Xa7 executor R gene durable resistance TALE bacterial blight rice

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Identification and characterization of putative CIPK genes in maize

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Journal of Genetics and Genomics 2011年第2期38卷 77-87页

作者： Xifeng chen Zhimin Gu Dedong Xin Liang Hao chengjie Liu Ji Huang bojun ma Hongsheng Zhang State Key Lab of Crop Genetics and Germplasm Enhaneement Nanjing Agricultural University Naajing 210095 China College of Chemistry and Life Sciences Zhejiang Normal University Jinhua 321004 China

Calcium （Ca） plays a crucial role as a second messenger in intracellular signaling elicited by developmental and environmental cues. Calcineurin B-like proteins （CBLs） and their target proteins, CBL-interacting protein kinases （CIPKs） have emerged as a key Ca^2＋-mediated signaling network in response to stresses in plants. Bioinformatic analysis was used to identify 43 putative ZmC1PK （Zea mays CIPK） genes in the genome of maize inbred line B73. Based on gene structures, these ZmCIPKs were divided into intron-rich and intron-poor groups. Phylogenetic analysis indicated that the ZmCIPK family had a high evolutionary relationship with the rice CIPK family of 30 members. Microarray data and RT-PCR assay showed that ZrnCIPK genes transcriptionally responded to abiotic stresses, and that 24, 31, 20 and 19 ZmCIPK genes were up-regulated by salt, drought, heat and cold stresses, respectively. There were different expression patterns of ZmCIPKs between cold-tolerant inbred line B73 and cold-sensitive inbred line Mo17 under cold stress. Our findings will aid further molecular dissection of biological functions of the CIPKs in maize, and provide new insight into the CBL--CIPK signaling network in plants.

关键词： maize CIPK family Evolution Expression Abiotic stresses

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In situ formed FeS_(2)@CoS cathode for long cycling life lithium-ion battery

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Chinese Physics B 2021年第8期30卷 529-534页

作者： Xin Wang bojun Wang Jiachao Yang Qiwen Ran Jian Zou Pengyu chen Li Li Liping Wang Xiaobin Niu School of materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China Tianmu Lake Institute of Advanced Energy Storage Technologies Changzhou 213300China

Pyrite FeS_(2)exhibits an ultrahigh energy density(1671 W·h·kg^(-1),for the reaction of FeS_(2)+4Li=Fe+2Li_(2)S)in secondary lithium-ion batteries,but its poor cycling stability,huge volume expansion,the shuttle effect of polysulfides,and slow kinetic properties limit its practical *** this work,we synthesize a composite structure material CoS on FeS_(2)surface(FeSx@CoS,1ma·h·g^(-1)(919 W·h·kg^(-1))after 60 cycles than 484 ma·h·g^(-1)(778 W·h·kg^(-1))of bare pyrite FeS_(2).This efficient strategy provides a valuable step toward the realization of high cycling stability FeS_(2)cathode materials for secondary lithium-ion batteries and enriches the basic understanding of the influence of FeS_(2)interfacial stability on its electrochemical performances.

关键词： Li-free cathodes pyrite lithium metal batteries first-principles calculation

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