Identification and characterization of putative CIPK genes in maize
Identification and characterization of putative CIPK genes in maize作者机构：State Key Lab of Crop Genetics and Germplasm Enhaneement Nanjing Agricultural University Naajing 210095 China College of Chemistry and Life Sciences Zhejiang Normal University Jinhua 321004 China
出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)
年 卷 期：2011年第38卷第2期
页 面：77-87页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种]
基 金：supported by the Natural Science Foundation of China(Nos.30771329 and 30800677) Natural Science Foundation of Zhejiang Province(No. Y3080359) the National Key Programs for Transgenic Crops(No.2009ZX08009-076B)
主 题：Maize CIPK family Evolution Expression Abiotic stresses
摘 要：Calcium （Ca） plays a crucial role as a second messenger in intracellular signaling elicited by developmental and environmental cues. Calcineurin B-like proteins （CBLs） and their target proteins, CBL-interacting protein kinases （CIPKs） have emerged as a key Ca^2＋-mediated signaling network in response to stresses in plants. Bioinformatic analysis was used to identify 43 putative ZmC1PK （Zea mays CIPK） genes in the genome of maize inbred line B73. Based on gene structures, these ZmCIPKs were divided into intron-rich and intron-poor groups. Phylogenetic analysis indicated that the ZmCIPK family had a high evolutionary relationship with the rice CIPK family of 30 members. Microarray data and RT-PCR assay showed that ZrnCIPK genes transcriptionally responded to abiotic stresses, and that 24, 31, 20 and 19 ZmCIPK genes were up-regulated by salt, drought, heat and cold stresses, respectively. There were different expression patterns of ZmCIPKs between cold-tolerant inbred line B73 and cold-sensitive inbred line Mo17 under cold stress. Our findings will aid further molecular dissection of biological functions of the CIPKs in maize, and provide new insight into the CBL--CIPK signaling network in plants.
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