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细胞周期蛋白依赖性激酶7抑制剂THZ1对脑胶质瘤干细胞干性的调控及机制

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中国组织工程研究 2025年第25期29卷 5374-5381页

作者：虎恩喜何文莹陶翔杜沛静王立斌宁夏医科大学第一临床医学院宁夏回族自治区银川市750004 宁夏医科大学基础医学院宁夏回族自治区银川市750004 华中科技大学协和深圳医院广东省深圳市518000

背景:THZ1是一种细胞周期蛋白依赖性激酶7的抑制剂,可以抑制多种肿瘤细胞的增殖,但THZ1是否可以通过Wnt/β-catenin信号通路影响脑胶质瘤干细胞的干性尚不清楚。目的:探究THZ1对脑胶质瘤细胞U87干性的影响及机制。方法:培养脑胶质瘤细胞... 详细信息

背景:THZ1是一种细胞周期蛋白依赖性激酶7的抑制剂,可以抑制多种肿瘤细胞的增殖,但THZ1是否可以通过Wnt/β-catenin信号通路影响脑胶质瘤干细胞的干性尚不清楚。目的:探究THZ1对脑胶质瘤细胞U87干性的影响及机制。方法:培养脑胶质瘤细胞U87形成干细胞球并通过Western blot验证干性相关蛋白的表达;采用CCK-8法检测THZ1作用于U87细胞的半数抑制浓度(IC_(50));通过克隆实验、划痕实验、Transwell迁移实验确定THZ1对U87细胞增殖、迁移的影响;分析THZ1处理对U87干细胞成球率及干细胞球体大小的影响;Western blot检测干性相关蛋白CD133、ABCG2、Nanog、OCT4、SOX2和上皮-间充质转化相关蛋白E-cadherin、N-cadherin、Occludin、Snail以及Wnt/β-catenin通路相关蛋白Axin1、β-Catenin、Wnt-5a、GSK3β、Cyclind-1、C-myc的表达变化。结果与结论:①与贴壁细胞相比,U87干细胞干性相关蛋白Nestin、CD133、ABCG2、Nanog、OCT4、SOX2表达明显升高;②与对照组相比,THZ1减弱了U87细胞的增殖和迁移能力;③THZ1抑制U87干细胞成球率及球体大小,下调干性相关蛋白的表达;④THZ1处理后,U87干细胞中N-cadherin、Snail蛋白表达降低,而E-cadherin、Occludin蛋白表达升高;(5)THZ1处理使U87干细胞中Wnt/β-catenin通路相关蛋白Axin1、β-Catenin、Wnt-5a、GSK3β、Cyclind-1、C-myc表达降低。结果表明:THZ1通过下调Wnt/β-catenin信号通路相关分子表达,抑制脑胶质瘤细胞U87的增殖、迁移,抑制U87干细胞成球能力、干性相关蛋白表达和上皮-间充质转化能力。

关键词： THZ1 脑胶质瘤肿瘤干细胞成球实验干性标志物 Wnt/β-catenin信号通路上皮-间充质转化增殖迁移

A Blast Shock Isolation System with MRFD and Its Semi-Active Control Analysis

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Transactions of Tianjin University 2006年第B09期12卷 65-69页

作者： LIU Jingbo wang libin DU Yixin wang Yan Department of Civil Engineering Tsinghua University Beijing 100084 China

To effectively reduce the damage to people and devices in civil defense engineering subjected to blast shock, a blast shock isolation system with magnetorheological fluid dampers (MRFD) is proposed. MRFD can provide continuously adjustable Coulomb friction and has many advantages for semi-active control. Numerical simulation of this isolation system is finished using Matlab simulink toolbox. General semi-active control algorithms are consided based on instantaneous optimal active control algorithm. And the results indicate that the shock isolation system can work efficiently, decreasing about 93% of the peak acceleration of the isolation floor.

关键词： blast shock isolation magnetorheological fluid damper semi-active control simulink

Cross-laminated Timber(CLT)in China:A State-of-the-Art

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Journal of Bioresources and Bioproducts 2019年第1期4卷 22-30,I0001页

作者： Hao LI Brad Jianhe wang Peixing WEI libin wang College of Civil Engineering Nanjing Forestry UniversityNanjing 210037China Ningbo Sino-Canada Low-Carbon Technology Research Institute Ninghai 315600China School of Landscape Architecture Jiangsu Vocational College of Agriculture and ForestryJurong 212400Chin

As a new type of green low-carbon engineered wood product,cross-laminated timber(CLT)is widely used in various types of wooden buildings in Europe and North America,and the number of high-rise wood construction is also *** on the introduction of the structural characteristics of the CLT and the development status of the CLT in developed countries,this paper focused on the review of the status of research and development of the CLT in China,with an emphasis on the breakthrough technologies of new bamboo-wood composite CLT ***,the prospects of the CLT in China were discussed.

关键词： cross-laminated timber(CLT) bamboo wood construction engineering application China

DIRECTION-OF-ARRIVAL ESTIMATION IN THE PRESENCE OF MUTUAL COUPLING BASED ON JOINT SPARSE RECOVERY

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Journal of Electronics(China) 2012年第5期29卷 408-414页

作者： wang libin Cui Chen Department of Information Engineering Electronic Engineering Institute Key Laboratory of Electronic Restricting Technique of Anhui Province

A novel Direction-Of-Arrival (DOA) estimation method is proposed in the presence of mutual coupling using the joint sparse recovery. In the proposed method, the eigenvector corresponding to the maximum eigenvalue of covariance matrix of array measurement is viewed as the signal to be represented. By exploiting the geometrical property in steering vectors and the symmetric Toeplitz structure of Mutual Coupling Matrix (MCM), the redundant dictionaries containing the DOA information are constructed. Consequently, the optimization model based on joint sparse recovery is built and then is solved through Second Order Cone Program (SOCP) and Interior Point Method (IPM). The DOA estimates are gotten according to the positions of nonzeros elements. At last, computer simulations demonstrate the excellent performance of the proposed method.

关键词： Direction-Of-Arrival (DOA) Uniform Linear Array (ULA) Mutual coupling Joint sparse recovery

Highly efficient H-bonding charge-transfer complex for microsupercapacitors under extreme conditions of low temperatures

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Journal of Energy Chemistry 2020年第12期29卷 182-189页

作者： libin wang Ting Shu Songtao Guo Shi Chen Yingjun Jiang Xianluo Hu State Key Laboratory of Materials Processing and Die Sf Mould Technology School of Materials Science and EngineeringHuazhong University of Science and TechnologyWuhan 430074HubeiChina

Owing to sluggish ionic mobility at low temperatures, supercapacitors, as well as other energy-storage devices, always suffer from severe capacity decay and even failure under extreme low-temperature circumstances. Solar-thermal-enabled self-heating promises an attractive approach to overcome this ***, we report a unique H-bonding charge-transfer complex with a high photothermal conversion efficiency of 79.5% at 405 nm based on chloranilic acid and albendazole. Integrated with a microsupercapacitor, the chloranilic acid-albendazole complex(CAC) film prompts an apparent temperature increase of 22.7 °C under 1 sun illumination at-32.6 °C, effectively elevating the working temperature of *** a result, the rate capability of the microsupercapacitor has been significantly improved with a 17-fold increase in capacitance at a current density of 60 μA cm^(-2), leading to outstanding low-temperature performances. Importantly, the integrated device is capable of working at a low temperature of-30 °C in the open air, which demonstrates the potential of CAC in practical applications for low-temperature ultracapacitive energy-storage devices.

关键词： Charge-transfer complexes Solar-thermal conversion Extreme conditions Microsupercapacitors Low temperature

Successful embryo transfer in Tianzhu white yak using standard protocol

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Science China(Life Sciences) 2007年第5期50卷 655-659页

作者： YU SiJiu JU XiangHong wang libin FAN JiangFeng Faculty of Veterinary Medicine Gansu Agriculture University Lanzhou 730070 China Faculty of Veterinary Medicine Gansu Agriculture University Lanzhou 730070 China Faculty of Veterinary Medicine Gansu Agriculture University Lanzhou 730070 China Faculty of Veterinary Medicine Gansu Agriculture University Lanzhou 730070 China

The present study was carried out to investigate the efficiency of superovulation, oestrus synchroni-zation, and embryo recovery in Tianzhu white yaks and also to confirm the pregnancy rate of black yaks, to which embryos collected from white yaks were transplanted. Forty-seven yaks were selected from different experiment groups, including 10 Tianzhu white female yaks (donor, group A) and 37 black female yaks (recipient, groups B and C). Superovulation of the donor was induced by the application procedure of CIDR-B + FSH + PG. Oestrus synchronization of recipients was induced using two meth-ods: group B was given the same treatment as group A, except that the follicle-stimulating hormone (FSH) injection was not administered, whereas group C was injected with cloprostenol only once when corpus luteum (corpora lutea) was (were) palpated. The results showed that the oestrous rates in group A were higher (80%) than those in group B (60%) and group C (44.5%). As for the efficiency of su-perovulation, it was indicated that the mean numbers (±SD) of total corpora lutea, follicles, viable (transferable), and degenerated embryos were 4.75 ± 2.19, 1.13 ± 0.83, 2.50 ± 1.31, and 1.38 ± 0.92, re-spectively. The mean embryo recovery rates were 55.6%. All together, 18 viable embryos of Tianzhu white yak were obtained and 12 of them were transplanted to 10 recipients. The pregnancy rate was 50% and the delivery rate was 40%.

关键词： Tianzhu white yak, superovulation, oestrus synchronization, embryo transfer

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Wuhan University Journal of Natural Sciences 2012年第5期17卷 414-420页

作者： LIU Shusheng wang libin GONG Zheng School of Computer Science South China Normal UniversityGuangzhou 510631GuangdongChina

The block cipher Threefish is the main component of Skein, which is based on ARX. Based on the efficient algorithms for calculating the differential of modular addition, we extend local collisions of Threefish-256 to more round by using related-key differential of addition in this paper. A related-key boomerang distinguish attack is proposed on 31-round Threefish-256 with a time complexity of 2^（234）.

关键词： Skein differential analysis related-key analysis Boomerang attack

MeCP2 deficiency promotes cell reprogramming by stimulating IGF1/AKT/mTOR signaling and activating ribosomal protein-mediated ceil cycle gene translation

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Journal of Molecular Cell Biology 2018年第6期10卷 515-526页

作者： Wei Zhang Guihai Feng libin wang Fei Teng Liu wang Wei Li Ying Zhang Qi Zhou State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

The generation of induced pturipotent stem cells (iPSCs)offers a great opportunity in research and regenerative *** current poor efficiency and incomplete mechanistic understanding of the reprogramming process hamper the clinical application of iPSCs. MeCP2 connects histone modification and DNA methyiation,which are key changes of somatic cell ***,the rote of MeCP2 in celt reprogramming has not been *** this study,we found that MeCP2 deficiency enhanced reprogramming efficiency and stimulated cell proliferation through regulating cell cycle protein expression in the earLy stage of ***2 deficiency enhanced the expression of ribosomal protein genes,thereby enhancing reprogramming efficiency through promoting the translation of ceLl cycle *** the end,MeCP2 deficiency stimulated IGF1/AKT/mTOR signaling and activated ribosomal protein gene *** together,our data indicate that MeCP2 deficiency promoted cell reprogramming through stimulating IGF1/AKT/mTOR signaling and activating ribosomal protein-mediated cell cycle gene translation in the early stage of reprogramming.

关键词： MeCP2 cell reprogramming IGFI/AKT/mTOR signaling ribosomal protein cell cycle

An ecohealth assessment of poultry production clusters (PPCs) for the livelihood and biosecurity improvement of small poultry producers in Asia

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Infectious Diseases of Poverty 2015年第1期4卷 49-57页

作者： libin wang Edi Basuno Tuan Nguyen Worapol Aengwanich Nyak Ilham Xiaoyun Li College of Humanities and Development Studies China Agricultural UniversityQinghua Donglu 17Beijing 100083China Indonesian Center for Agriculture Socio Economic and Policy Studies J1 Ahmad Yani 7016161 BogorIndonesia Center for Agricultural Policy Institute of Policy and Strategy for Agriculture and Rural DevelopmentHanoiVietnam Faculty of Veterinary Sciences Mahasarakham UniversityMaha Sarakham 440000Thailand

Background:Poultry production cluster(PPC)programs are key strategies in many Asian countries to engage small commercial poultry producers in high-value production chains and to control infectious poultry *** study assessed the multiple impacts of PPCs through a transdisciplinary ecohealth approach in four Asian countries,and drew the implications for small producers to improve their livelihoods and reduce the risk of spreading infectious diseases in the poultry ***:The data collection combined both quantitative and qualitative *** comprised:formal structured household survey questionnaires,measuring the biosecurity level of poultry farms with a biosecurity score card;and key informant *** statistics were used to process the quantitative data and a content analysis was used to process the qualitative ***:This research found that poultry farms in clusters do not necessarily have better economic performance than those outside *** farmers in PPCs only consider them to be an advantage for expanding the scale of their poultry operations and improving household incomes,and they are less concerned about-and have limited capacities to-enhancing biosecurity and environmental *** measured the biosecurity level of farms in PPCs through a 14-item checklist and found that biosecurity is generally very low across all sample *** increased flies,mosquitoes,rats,and smells in and around PPCs not only pollute the environment,but also cause social conflicts with the surrounding ***:This research concluded that a poultry cluster,mainly driven by economic objectives,is not necessarily a superior model for the control of infectious *** level of biosecurity in PPCs was found to be *** the intensity of poultry operations in PPCs(farms are densely packed into clusters),and the close proximity to residential areas of some PPCs,the risk of spreading infectious diseases,in fact,incre

关键词： Poultry production cluster(PPC) Small producers Biosecurity Infectious diseases Livelihood Ecohealth