Successful embryo transfer in Tianzhu white yak using standard protocol

作     者：YU SiJiu JU XiangHong WANG LiBin FAN JiangFeng 

作者机构：Faculty of Veterinary Medicine Gansu Agriculture University Lanzhou 730070 China Faculty of Veterinary Medicine Gansu Agriculture University Lanzhou 730070 China Faculty of Veterinary Medicine Gansu Agriculture University Lanzhou 730070 China Faculty of Veterinary Medicine Gansu Agriculture University Lanzhou 730070 China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2007年第50卷第5期

页      面：655-659页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：Supported by Gansu Science Foundation of Application of Biological Techniques in Agriculture (Grant No. GNSW-2004-07) Science Foundation of Gansu Province (Grant No. 2GS042-A41-001-05) 

主　　题：Tianzhu white yak, superovulation, oestrus synchronization, embryo transfer 

摘      要：The present study was carried out to investigate the efficiency of superovulation, oestrus synchroni-zation, and embryo recovery in Tianzhu white yaks and also to confirm the pregnancy rate of black yaks, to which embryos collected from white yaks were transplanted. Forty-seven yaks were selected from different experiment groups, including 10 Tianzhu white female yaks (donor, group A) and 37 black female yaks (recipient, groups B and C). Superovulation of the donor was induced by the application procedure of CIDR-B + FSH + PG. Oestrus synchronization of recipients was induced using two meth-ods: group B was given the same treatment as group A, except that the follicle-stimulating hormone (FSH) injection was not administered, whereas group C was injected with cloprostenol only once when corpus luteum (corpora lutea) was (were) palpated. The results showed that the oestrous rates in group A were higher (80%) than those in group B (60%) and group C (44.5%). As for the efficiency of su-perovulation, it was indicated that the mean numbers (±SD) of total corpora lutea, follicles, viable (transferable), and degenerated embryos were 4.75 ± 2.19, 1.13 ± 0.83, 2.50 ± 1.31, and 1.38 ± 0.92, re-spectively. The mean embryo recovery rates were 55.6%. All together, 18 viable embryos of Tianzhu white yak were obtained and 12 of them were transplanted to 10 recipients. The pregnancy rate was 50% and the delivery rate was 40%.