检索结果-南通市图书馆

Astragalus Polysaccharide Enhances Voriconazole Metabolism under Inflammatory Conditions through the Gut Microbiota

在线全文

学校读者我要写书评

暂无评论

Journal of Clinical and Translational Hepatology 2024年第5期12卷 481-495页

作者： Xiaokang Wang Xianjing Hu Chunxiao Ye Jingqian Zhao Shing Cheng Tan liangbin zhou Chenyu Zhao Kit Hang Wu Xixiao Yang Jinbin Wei Maoxun Yang The Marine Biomedical Research Institute of Guangdong Zhanjiang ZhanjiangGuangdongChina Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs Guangdong Medical UniversityDongguanGuangdongChina Department of Pharmacy Shenzhen Longhua District Central HospitalShenzhenGuangdongChina Dongguan Key Laboratory of Chronic Inflammatory Diseases The First Dongguan Affiliated HospitalGuangdong Medical UniversityDongguanGuangdongChina Department of Pharmacy The Second Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdongChina Department of Pharmacy Shenzhen Hospital of Southern Medical UniversityShenzhenGuangdongChina UKM Medical Molecular Biology Institute Universiti Kebangsaan MalaysiaKuala LumpurMalaysia Department of Biomedical Engineering Faculty of EngineeringThe Chinese University of Hong KongHong Kong SARChina Department of Pharmacy Nossa Senhora do Carmo-Lago Health CentreHealth BureauMacaoChina Pharmaceutical College Guangxi Medical UniversityNanningGuangxiChina

Background and Aims:Voriconazole(VRC),a widely used antifungal drug,often causes hepatotoxicity,which presents a significant clinical *** studies demonstrated that Astragalus polysaccharide(APS)can regulate VRC metabolism,thereby potentially mitigating its hepatotoxic *** this study,we aimed to explore the mechanism by which APS regulates VRC ***:First,we assessed the association of abnormal VRC metabolism with hepatotoxicity using the Roussel Uclaf Causality Assessment Method ***,we conducted a series of basic experiments to verify the promotive effect of APS on VRC *** in vitro and in vivo assays,including cytokine profiling,immunohistochemistry,quantitative polymerase chain reaction,metabolite analysis,and drug concentration measurements,were performed using a lipopolysaccharideinduced rat inflammation ***,experiments such as intestinal biodiversity analysis,intestinal clearance assessments,and Bifidobacterium bifidum replenishment were performed to examine the ability of *** to regulate the expression of the VRC-metabolizing enzyme CYP2C19 through the gut–liver ***:The results indicated that APS does not have a direct effect on ***,the assessment of gut microbiota function revealed that APS significantly increases the abundance of ***,which could lead to an anti-inflammatory response in the liver and indirectly enhance VRC *** dual-luciferase reporter gene assay revealed that APS can hinder the secretion of pro-inflammatory mediators and reduce the inhibitory effect on CYP2C19 transcription through the nuclear factor-κB signaling ***:The study offers valuable insights into the mechanism by which APS alleviates VRC-induced liver damage,highlighting its immunomodulatory influence on hepatic tissues and its indirect regulatory control of VRC-metabolizing enzymes within hepatocytes.

关键词： Astragalus polysaccharide Voriconazole Metabolism RUCAM Bifidobacterium bifidum Anti-inflammatory responses Gut Microbiota Hepatotoxicity

Functionalized Hydrogels for Articular Cartilage Tissue Engineering

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Engineering 2022年第6期8卷 71-90页

作者： liangbin zhou Peng Guo Matteo D’Este Wenxue Tong Jiankun Xu Hao Yao Martin J.Stoddart Gerjo J.V.M.van Osch Kevin Ki-Wai Ho Zhen Li Ling Qin Musculoskeletal Research Laboratory of Department of Orthopaedics&Traumatology Faculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Innovative Orthopaedic Biomaterials and Drug Translational Research Laboratory Li Ka Shing Institute of Health SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong 999077China AO Research Institute Davos DavosCH 7270Switzerland Innovation Platform of Regeneration and Repair of Spinal Cord and Nerve Injury Department of Orthopaedic SurgeryThe Seventh Affiliated Hospital of Sun Yat-sen UniversityShenzhen 518000China Department of Orthopaedics and Sports Medicine&Department of Otorhinolaryngology Erasmus MCUniversity Medical CenterRotterdam 3000 CAthe Netherlands f Department of Biomechanical EngineeringDelft University of TechnologyDelft 2600 AAthe Netherlands Centre for Translational Medicine Research and Development Shenzhen Institute of Advanced TechnologyThe Chinese Academy of SciencesShenzhen 518000China

Articular cartilage(AC)is an avascular and flexible connective tissue located on the bone surface in the diarthrodial *** defects are common in the knees of young and physically active *** of the lack of suitable tissue-engineered artificial matrices,current therapies for AC defects,espe-cially full-thickness AC defects and osteochondral interfaces,fail to replace or regenerate damaged carti-lage *** rapid research and development advancements in AC tissue engineering(ACTE),functionalized hydrogels have emerged as promising cartilage matrix substitutes because of their favor-able biomechanical properties,water content,swelling ability,cytocompatibility,biodegradability,and lubricating *** can be rationally designed and conveniently tuned to simulate the extracel-lular matrix of *** article briefly introduces the composition,structure,and function of AC and its defects,followed by a comprehensive review of the exquisite(bio)design and(bio)fabrication of func-tionalized hydrogels for AC ***,we summarize the challenges encountered in functionalized hydrogel-based strategies for ACTE both in vivo and in vitro and the future directions for clinical translation.

关键词： Articular cartilage Functionalized hydrogels Cartilage repair Cartilage tissue engineering Clinical translation

Engineered biochemical cues of regenerative biomaterials to enhance endogenous stem/progenitor cells(ESPCs)-mediated articular cartilage repair

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第8期26卷 490-512页

作者： liangbin zhou Jietao Xu Andrea Schwa Wenxue Tong Jiankun Xu Lizhen Zheng Ye Li Zhuo Li Shunxiang Xu Ziyi Chen Li Zou Xin Zhao Gerjo J.V.Mvan Osch Chunyi Wen Ling Qin Musculoskeletal Research Laboratory of Department of Orthopaedics and Traumatology&Innovative Orthopaedic Biomaterials and Drug Translational Research Laboratory of Li Ka Shing Institute of Health Sciences Faculty of MedicineThe Chinese University of Hong Kong999077Hong Kong SARChina Department of Biomedical Engineering Faculty of EngineeringThe Hong Kong Polytechnic University999077Hong Kong SARChina Department of Orthopaedics and Sports Medicine Erasmus MCUniversity Medical Center Rotterdam3015 GDRotterdamthe Netherlands Department of Oral and Maxillofacial Surgery Erasmus MCUniversity Medical Center Rotterdam3015 GDRotterdamthe Netherlands Centre for Regenerative Medicine and Health Hong Kong Institute of Science&InnovationChinese Academy of Sciences-CRMH999077Hong Kong SARChina Department of Biomedical Engineering Faculty of EngineeringThe Chinese University of Hong Kong999077Hong Kong SARChina Department of Otorhinolaryngology Erasmus MCUniversity Medical Center Rotterdam3015 GDRotterdamthe Netherlands Department of Biomechanical Engineering Faculty of MechanicalMaritime and Materials EngineeringDelft University of Technology(TU Delft)2600 AADelftthe Netherlands Centre for Translational Medicine Research and Development Shenzhen Institute of Advanced TechnologyThe Chinese Academy of Sciences518000ShenzhenChina

As a highly specialized shock-absorbing connective tissue,articular cartilage(AC)has very limited self-repair capacity after traumatic injuries,posing a heavy socioeconomic *** clinical therapies for small-to medium-size focal AC defects are well-developed endogenous repair and cell-based strategies,including microfracture,mosaicplasty,autologous chondrocyte implantation(ACI),and matrix-induced ACI(MACI).However,these treatments frequently result in mechanically inferior fibrocartilage,low cost-effectiveness,donor site morbidity,and short-term *** prompts an urgent need for innovative approaches to pattern a pro-regenerative microenvironment and yield hyaline-like cartilage with similar biomechanical and biochemical properties as healthy native *** regenerative biomaterials can create a favorable local environment for AC repair without causing relevant regulatory and scientific concerns from cell-based treatments.A deeper understanding of the mechanism of endogenous cartilage healing is furthering the(bio)design and application of these ***,the utilization of regenerative biomaterials to magnify the repairing effect of joint-resident endogenous stem/progenitor cells(ESPCs)presents an evolving improvement for cartilage *** review starts by briefly summarizing the current understanding of endogenous AC repair and the vital roles of ESPCs and chemoattractants for cartilage *** several intrinsic hurdles for regenerative biomaterials-based AC repair are *** recent advances in novel(bio)design and application regarding regenerative biomaterials with favorable biochemical cues to provide an instructive extracellular microenvironment and to guide the ESPCs(***,migration,proliferation,differentiation,matrix production,and remodeling)for cartilage repair are ***,this review outlines the future directions of engineering the next-generation regenerative biomaterials toward ultim

关键词： Regenerative biomaterials Endogenous stem/progenitor cells(ESPCs) Articular cartilage(AC)repair Biochemical cues

欧盟体外诊断医疗器械分类监管体系变化与思考

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国医疗器械杂志 2021年第6期45卷 674-679页

作者：周良彬崔乐程娟黄颖广东省医疗器械质量监督检验所广州市510663 中国食品药品检定研究院北京市100050

分析对比欧盟新法规IVDR与旧指令IVDD及相关指南文件中IVD产品分类监管体系,探讨其中监管变革思路,为行业人员了解掌握新的欧盟IVD分类体系提供参考,以供我国进行中的分类管理改革工作作为借鉴。

关键词：体外诊断器械分类监管体系法规欧盟

在线全文

学校读者我要写书评

暂无评论

转基因何首乌毛状根生物转化瑞香素的研究

中国中药杂志 2011年第3期36卷 351-355页

作者：邓文娟周良彬于荣敏暨南大学药学院广东广州510632

目的:利用转基因何首乌毛状根对瑞香素(I)进行生物转化研究,对其生物转化产物进行分离、鉴定,并建立其产物的时效曲线图。方法:将瑞香素投入何首乌毛状根悬浮培养体系中,共培养36 h后,利用TLC和HPLC进行产物检测,硅胶柱层析法分离纯化,... 详细信息

目的:利用转基因何首乌毛状根对瑞香素(I)进行生物转化研究,对其生物转化产物进行分离、鉴定,并建立其产物的时效曲线图。方法:将瑞香素投入何首乌毛状根悬浮培养体系中,共培养36 h后,利用TLC和HPLC进行产物检测,硅胶柱层析法分离纯化,核磁共振技术进行结构鉴定,利用HPLC考察共培养时间对转化产物(Ⅱ)、降解产物含量的影响及分析转化产物降解的原因。结果:瑞香素在何首乌毛状根悬浮培养体系中发生了生物转化反应。分离鉴定出一个产物:瑞香素-8-O-β-D-葡萄糖苷(Ⅱ)。何首乌毛状根悬浮培养体系转化生成Ⅱ的最佳共培养时间为36 h,总摩尔转化率为32.11%。蔗糖-水培养基(仅含有蔗糖和水)可以使何首乌毛状根悬浮培养体系转化产率大幅增加,总摩尔转化率达72.44%。初步推断产物(Ⅱ)的降解与MS培养基中金属离子有关。结论:使用何首乌毛状根悬浮培养体系可大量生物合成天然药材中含量极低的活性化合物——瑞香素-8-O-β-D-葡萄糖苷。

关键词：何首乌毛状根生物转化瑞香素瑞香素-8-O-β-D-葡萄糖苷

2,3-二氢环戊二烯香豆素类化合物对转基因何首乌毛状根生长及其二苯乙烯苷累积的影响

在线全文

学校读者我要写书评

暂无评论

中国中药杂志 2012年第9期37卷 1197-1201页

作者：刘玲周良彬张明华于荣敏暨南大学中药生物技术研究所广东广州510632 暨南大学药学院天然药物化学研究室广东广州510632

目的:考察2,3-二氢环戊二烯香豆素类化合物对何首乌毛状根生长的影响,并研究其对毛状根中有效成分二苯乙烯苷累积的刺激作用。方法:将2,3-二氢环戊二烯香豆素类化合物在不同的培养时间加入到毛状根中,共培养特定时间后测定其二苯乙烯苷... 详细信息

目的:考察2,3-二氢环戊二烯香豆素类化合物对何首乌毛状根生长的影响,并研究其对毛状根中有效成分二苯乙烯苷累积的刺激作用。方法:将2,3-二氢环戊二烯香豆素类化合物在不同的培养时间加入到毛状根中,共培养特定时间后测定其二苯乙烯苷的含量及生长量,并通过考察不同浓度和继代次数确定最佳实验条件。结果:加入香豆素类化合物的最佳时间为预培养第4天,最佳质量浓度为0.025 g.L-1,毛状根的生长量有较大提高,与对照组相比二苯乙烯苷的含量增加4倍左右。结论:2,3-二氢环戊二烯香豆素类化合物作为一类非生物诱导子能够促进何首乌毛状根的生长及有效成分二苯乙烯苷的累积。

关键词： 2,3-二氢环戊二烯香豆素诱导作用何首乌毛状根二苯乙烯苷

益赛普联合复方风湿宁片用于难治性强直性脊柱炎的疗效研究

在线全文

学校读者我要写书评

暂无评论

基层医学论坛 2014年第19期18卷 2464-2465页

作者：周良斌胡昌盛黄冈职业技术学院附属医院湖北黄冈438000 黄冈职业技术学院医药卫生学院湖北黄冈438000

目的分析益赛普联合复方风湿宁片治疗难治性强直性脊柱炎的临床效果。方法将2011年2月—2012年9月收治的60例难治性强直性脊柱炎患者,随机分为治疗组和对照组。对照组采用益赛普治疗,治疗组在对照组的基础上联合复方风湿宁片治疗,监测2... 详细信息

目的分析益赛普联合复方风湿宁片治疗难治性强直性脊柱炎的临床效果。方法将2011年2月—2012年9月收治的60例难治性强直性脊柱炎患者,随机分为治疗组和对照组。对照组采用益赛普治疗,治疗组在对照组的基础上联合复方风湿宁片治疗,监测2组患者的观察指标。结果 2组患者的各项指标在治疗后均升高,但治疗组改善更明显(P<0.01)。临床症状缓解的时间分别为(38.1±2.8)d、(67.5±4.2)d,治疗组明显优于对照组(P<0.01)。结论难治性强直性脊柱炎应用益赛普联合复方风湿宁片治疗,能够明显缓解症状、控制复发,疗效显著,值得临床推广应用。

关键词：强直性脊柱炎难治性益赛普复方风湿宁片

腹腔内注射丹参多酚酸治疗慢性盆腔炎44例临床疗效观察

在线全文

学校读者我要写书评

暂无评论

内蒙古中医药 2014年第13期33卷 13-14页

作者：周良斌胡昌盛黄冈职业技术学院 438000

目的:应用丹参多酚酸注射液腹腔内注射治疗慢性盆腔炎的疗效。方法:2012年月至2013年11月我院收治的慢性盆腔炎患者88例,随机分为治疗组和对照组两组各44例,治疗组应用丹参多酚酸注射液,对照组庆应用大霉素、地塞米松、甲硝唑。两组均... 详细信息

目的:应用丹参多酚酸注射液腹腔内注射治疗慢性盆腔炎的疗效。方法:2012年月至2013年11月我院收治的慢性盆腔炎患者88例,随机分为治疗组和对照组两组各44例,治疗组应用丹参多酚酸注射液,对照组庆应用大霉素、地塞米松、甲硝唑。两组均采用腹腔内注射的治疗方法。疗程为14天。结果:治疗组有效率为97.73%,对照组有效率为75.00%。治疗组疗效明显高于对照组(X2=9.65 P<0.01)。结论:丹参多酚酸腹腔内注射治疗慢性盆腔炎疗效确切,安全性能好。

关键词：腹腔内注射丹参多酚酸慢性盆腔炎

利奈唑胺治疗耐药性金黄色葡萄球菌肺炎的效果观察

在线全文

学校读者我要写书评

暂无评论

基层医学论坛 2014年第1期18卷 12-14页

作者：周良斌黎春红黄冈职业技术学院湖北黄冈438000

目的观察比较利奈唑胺和万古霉素治疗耐甲氧西林金黄色葡萄球菌(MRSA)呼吸机相关性肺炎(VAP)的临床效果和不良反应。方法将88例MRSA所致VAP患者随机分为2组,治疗组和对照组各44例。治疗组采用利奈唑胺治疗,对照组采用万古霉素治疗。2组... 详细信息

目的观察比较利奈唑胺和万古霉素治疗耐甲氧西林金黄色葡萄球菌(MRSA)呼吸机相关性肺炎(VAP)的临床效果和不良反应。方法将88例MRSA所致VAP患者随机分为2组,治疗组和对照组各44例。治疗组采用利奈唑胺治疗,对照组采用万古霉素治疗。2组疗程均为14 d,观察2组的临床疗效和不良反应。结果MRSA清除率治疗组为79.55%,对照组为56.82%;治疗组总有效率为74.00%,对照组为52.00%,2组比较,治疗组清除率、总有效率均优于对照组(P0.05)。结论利奈唑胺对MRSA所致VAP有较好的临床疗效,毒副作用与万古霉素相当。

关键词：呼吸机相关性肺炎耐甲氧西林金黄色葡萄球菌利奈唑胺万古霉素