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富油微藻-真菌共培养资源化处理模拟畜禽养殖废水的效能研究

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环境科学研究 2023年第4期36卷 715-723页

作者：李双喜朱联东武汉大学资源与环境科学学院湖北武汉430079

为探究富油微藻-真菌共培养处理畜禽养殖废水的可行性,构建小球藻(Chlorella vulgaris)和米曲霉菌(Aspergillus oryzae)的共培养体系,通过调节不同比例的微藻真菌初始接种量,对模拟畜禽养殖废水污染物去除、藻菌结合与采收、生物质干质... 详细信息

为探究富油微藻-真菌共培养处理畜禽养殖废水的可行性,构建小球藻(Chlorella vulgaris)和米曲霉菌(Aspergillus oryzae)的共培养体系,通过调节不同比例的微藻真菌初始接种量,对模拟畜禽养殖废水污染物去除、藻菌结合与采收、生物质干质量与生化组分以及生物柴油生产能力进行系统研究.结果表明:①C.vulgaris和A.oryzae在废水处理过程中具有协同效应,与C.vulgaris单独处理相比,共培养更有利于污染物的去除.最优藻菌接种比例为25∶1,TN、NH_(4)^(+)-N、TP、COD和Cu(Ⅱ)的去除率分别达72.51%、71.19%、92.23%、91.47%和90.38%.藻菌共培养对3种磺胺类药物(SAs)也具有显著的生物去除效果,磺胺二甲嘧啶(SMZ)、磺胺甲恶唑(SMX)和磺胺间甲氧嘧啶(SMM)的去除率分别达57.61%、58.31%和50.48%.②丝状真菌易于与微藻形成球状体,在菌丝强力支撑作用下,C.vulgaris在废水中呈现悬浮状态,提高污染物的去除率,并实现对微藻的高效采收,最高采收效率为76.91%,采收后的总生物质干质量高达1.26 g/L,总叶绿素含量达15.99 mg/g.③藻菌生物质中蛋白质、多糖和油脂的含量分别为111、136和249 mg/g,而脂肪酸组分主要以C16∶0(棕榈酸)、C18∶1(油酸)和C18:2(亚油酸)为主,有利于生物柴油生产.研究显示,C.vulgaris和A.oryzae共培养不仅能有效去除废水中的各类污染物,而且能实现生物质的资源化利用,在畜禽养殖废水处理中具有潜在的工程应用价值.

关键词：微藻-真菌共培养畜禽养殖废水磺胺类药物 Cu(Ⅱ) 生物柴油生产

Red oil A5 inhibits proliferation and induces apoptosis in pancreatic cancer cells

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World Journal of Gastroenterology 2004年第1期10卷 105-111页

作者： Mi-liandong Xian-ZhongDing ThomasE.Adrian AffiliatedTaizhouHospital WenzhouMedicalCollegeLinhai317000ZhejiangProvinceChina NorthwesternUniversityMedicalSchool ChicagoIL60611-3008U.S.A

AIM: To study the effect of red oil A5 on pancreatic cancer cells and its possible mechanisms. METHODS: Effect of different concentrations of red oil A5 on proliferation of three pancreatic cancer cell lines, AsPC-1, MiaPaCa-2 and S2013, was measured by 3H-methyl thymidine incorporation. Time-dependent effects of 1:32 000 red oil A5 on proliferation of three pancreatic cancer cell lines, were also measured by ^3H-methyl thymidine incorporation, and Time-course effects of 1:32 000 red oil A5 on cell number.The cells were counted by Z1-Coulter Counter. Fiowcytometric analysis of cellular DNA content in the control and red oil A5 treated AsPC-1, MiaPaCa-2 and $2013 cells,were stained with propidium iodide. TUNEL assay of red oil A5-induced pancreatic cancer cell apoptosis was performed. Western blotting of the cytochrome c protein in AsPC-1, MiaPaCa-2 and $2013 cells treated 24 hours with 1:32 000 red oil A5 was performed. Proteins in cytosolic fraction and in mitochondria fraction were extracted. Proteins extracted from each sample were electrophoresed on SDS-PAGE gels and then were transferred to nitrocellulose membranes. Cytochrome c was identified using a monoclonal cytochrome c antibody. Western blotting of the caspase-3 protein in AsPC-1, MiaPaCa-2 and S2013 cells treated with 1:32 000 red oil A5 for 24 hours was carried out. Proteins in whole cellular lysates were electrophoresed on SDS-PAGE gels and then transferred to nitrocellulose membranes. Caspase-3 was identified using a specific antibody. Western blotting of polyADP ribose polymerase (PARP) protein in AsPC-1, MiaPaCa2 and S2013 cells treated with 1:32 000 red oil A5 for 24 hours was performed. Proteins in whole cellular lysates were separated by electrophoresis on SDS-PAGE gels and then transferred to nitrocellulose membranes. PARP was identified by using a monoclonal antibody. RESULTS: Red oil A5 caused dose- and time-dependent inhibrdon of pancreatic cancer cell proliferation. Propidium iodide DNA staining showed an increase of the sub-G0/G1 cell po

关键词：胰腺癌细胞凋亡细胞增殖红油A5 药理作用 TUNEL法

An activatable ratiometric near-infrared fluorescent probe for hydrogen sulfide imaging in vivo

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Science China Chemistry 2020年第5期63卷 741-750页

作者： Luyan Wu Wenhui Zeng liandong Feng Yuxuan Hu Yidan Sun Yingxiao Yan Hong-Yuan Chen Deju Ye State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Chemistry and Biomedicine Innovation Center(ChemBIC) Nanjing UniversityNanjing 210023China

Ratiometric fluorescent probes hold great promise for in vivo imaging;however,stimuli-activatable ratiometric probes with fluorescence emissions in near-infrared(NIR)region are still very few.Herein,we report a hydrogen sulfide(H_2S)-activatable ratiometric NIR fluorescent probe(1-SPN)by integrating a H_2S-responsive NIR fluorescent probe 1 into a H_2S-inert poly[2,6-(4,4-bis-(2-ethylhexyl)-4 H-cyclopenta[2,1-b;3,4-b′]dithiophene)-alt-4,7(2,1,3-benzothiadiazole)](PCPDTBT)-based NIR semiconducting polymer nanoparticle(SPN).1-SPN shows"always on"PCPDTBT fluorescence at 830 nm and weak probe 1 fluorescence at 725 nm under excitation at 680 nm.The ratio of NIR fluorescence intensities between 725 and 830 nm(I_(725)/I_(830))is small.Upon interaction with H_2S,the fluorescence at 725 nm is rapidly switched on,resulting in a large enhancement of I_(725)/I_(830),which is allowed for sensitive visualization and quantification of H_2S concentrations in living cells.Taking advantage of enhanced tissue penetration depth of NIR fluorescence,1-SPN is also applied for real-time ratiometric fluorescence imaging of hepatic and tumor H_2S in living mice.This study demonstrates that activatable ratiometric NIR fluorescent probes hold great potential for in vivo imaging.

关键词： ratiometric probe activatable probe near-infrared fluorescence molecular imaging H2S

Oil A induces apoptosis of pancreatic cancer cells via caspase activation, redistribution of cell cycle and GADD expression

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World Journal of Gastroenterology 2003年第12期9卷 2745-2750页

作者： Mi-liandong Yue-ChunZhu JohnV.Hopkins AffiliatedTaizhouHospital WenzhouMedicalCollegeLinhai317000ZhejiangProviceChina NorthwesternUniversityMedicalSchool ChicagoIL60611-3008U.S.A

AIM: To explore the mechanisms of effects of oil A on apoptosis of human pancreatic cancer cells.METHODS: Cellular DNA content was analyzed by flow cytometry. Western blotting was used for caspase-3 and PARP, caspase-7, caspase-9, cytochrome c, Bcl-2, Bax, Mcl-1, cyclinA, cyclin B1, cyclin D1, cyclin E, CDK2, CDK4, CDK6,P21, P27,GADD45, GADD153.RESULTS: The caspase-3, caspase-7, and caspase-9activities were significantly increased as well as the cleavage of caspase-3, downstream substrate poly-ADP ribose polymerase (PARP) was induced. The amount of cytochrome c in the cytosolic fraction was increased, while the amount of cytochrome c in the mitochondrial fraction was decreased after oil A treatment. The anti-apoptosis proteins Bcl-2and Mci-1 were decreased in parallel and Bax increased,indicating that Bcl-2 family proteins-mitochondria-caspase cascade was responsible for oil-induced apoptosis. The proportion of cells in the G0/G1 decreased in MiaPaCa-2and AsPC-1 cells after the treatment of oil A for 24 hours.The number of cells in S phase was increased in two cancer cell lines at 24 hours. Therefore, cells were significantly accumulated in G2/M phase. The cells with a sub-G0/G1DNA content, a hallmark of apoptosis, were seen at 24 hours both in MiaPaCa-2 and AsPC-1 cells following exposure to oil A. The expression of cyclin A and cyclin B1 was slightly decreased and cyclin D1 levels were markedly lowered in MiaPaCa-2 cells. The expression of cyclin A and cyclin B1 was markedly decreased and cyclin D1 levels were slightly lowered in AsPC-1 cells, while cydin E was not affected and the levels of CDK2, CDK4, and CDK6 were unchanged in MiaPaCa-2 and AsPC-1 cells. In response to oil A, P21 expression was increased, but P27 expression was not affected. The expression of both GADD45 and GADD153was increased in two cell lines following oil A treatment.CONCLUSION: Oil A induces apoptosis of pancreatic cancer cells via activating caspase cascade, modifying cell cycle progress and changing cell cycle-regulating proteins and

关键词：胰腺癌细胞凋亡血流细胞计数载脂蛋白 GADD 抗癌作用 A油

Red Electrophoretic Particles Based on Fe_2O_3 Nanoparticles for Electronic Inks: Design, Preparation and Properties

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Transactions of Tianjin University 2015年第3期21卷 244-249页

作者：邓联东张晶董岸杰 School of Chemical Engineering and Technology Tianjin University

In order to modify inorganic particles as chromatic electrophoretic particles, an approach was designed and used to prepare Fe2O3 red electrophoretic particles. These Fe2O3-cationic hybrid nanoparticles(Fe2O3-CHNPs)were prepared through Fe2O3 core covered with polymer shell which was composed of SiO 2 and P(DMAEMA-co-HMA)by using atom transfer radical polymerization(ATRP)technique. The Si O2-coating could introduce the functional group on the surfaceof inorganic particles, through which the polymer shell could be formed by using ATRP technique. The results of Fourier transform infrared spectra(FT-IR), X-ray photoelectron spectroscopy(XPS)and thermal gravimetric analysis(TGA)confirmed the chemical compositions of Fe2O3-CHNPs; the images of transmission electron microscopy(TEM)indicated the core-shell structure of Fe2O3-CHNPs; the measurements of dynamic light scattering(DLS)showed a 253.7 nm average particle size with narrow size distribution; and the zeta potential measurements identified the high chargeability of Fe2O3-CHNPs. Furthermore, the resulting nanoparticles were successfully applied in the electrophoretic display cell, which demonstrated that it was an effective approach to preparing chromatic electrophoretic particles.

关键词：纳米粒子 Fe2O3 电泳制备电子墨水设计原子转移自由基聚合 X射线光电子能谱

生物医用纳米材料在增强肿瘤细胞免疫原性死亡中的应用

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科学通报 2022年第13期67卷 1436-1448页

作者：庞小雨董岸杰邓联东程亚亚邓宏章天津大学化工学院天津300350 西安电子科技大学生命科学技术学院西安710126

激活机体的适应性免疫反应是提升长期抗肿瘤疗效的关键因素.化学治疗、放射治疗、光动力治疗(photodynamic therapy,PDT)等方法可诱导肿瘤细胞发生凋亡并伴随着免疫原性死亡(immunogenic cell death,ICD).ICD引起的损伤相关分子模式(dam... 详细信息

激活机体的适应性免疫反应是提升长期抗肿瘤疗效的关键因素.化学治疗、放射治疗、光动力治疗(photodynamic therapy,PDT)等方法可诱导肿瘤细胞发生凋亡并伴随着免疫原性死亡(immunogenic cell death,ICD).ICD引起的损伤相关分子模式(damage associated molecular patterns,DAMPs)分子的暴露或释放可刺激免疫系统发挥抗肿瘤免疫作用,招募抗原递呈细胞(antigen-presenting cell,APC),激活T细胞适应性免疫应答.因此,在肿瘤免疫治疗中ICD诱导剂可以改善其治疗效果,从而提高患者生存率.越来越多的研究表明,基于纳米材料的癌症治疗平台不仅可以实现特定肿瘤微环境(tumor microenvironment,TME)刺激响应的药物控制释放,并且其可以调节TME进而诱导肿瘤细胞发生ICD,这撞击出了癌症纳米医学的新火花.自此以后,设计和构建增强肿瘤ICD效应的生物医用材料的相关研究受到了极大关注.基于此背景,本文首先介绍了肿瘤ICD的成因以及纳米材料在诱导肿瘤ICD中发挥的多重功能.其次,阐述了纳米材料在调控TME,改善肿瘤治疗效果方面所具有的巨大潜力.最后,对纳米材料在诱导肿瘤ICD研究中所面临的挑战也进行了展望性的深入探讨.

关键词：纳米材料免疫原性死亡药物递送免疫治疗联合治疗

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劣质原料生产低硫低凝柴油的工艺研究

石油炼制与化工 1998年第5期29卷 8-11页

作者： yang liandong Zhou Jianwu (Research Institute of Petroleum Processing, Beijing 100083)

采用加氢精制－加氢脱蜡工艺，由青海原油高含蜡、高含氮的直馏常三线馏分油及新疆混合原油重油催化裂化柴油等劣质原料生产低硫（≤０．０５％）、低凝点（冷滤点符合－３５号柴油规格）的合格柴油产品。该工艺有效地缓和了柴油加氢脱... 详细信息

采用加氢精制－加氢脱蜡工艺，由青海原油高含蜡、高含氮的直馏常三线馏分油及新疆混合原油重油催化裂化柴油等劣质原料生产低硫（≤０．０５％）、低凝点（冷滤点符合－３５号柴油规格）的合格柴油产品。该工艺有效地缓和了柴油加氢脱蜡的反应条件，对提高柴油产品收率、延长催化剂使用周期及提高柴油产品的质量极为有利。该工艺对原料适应性好，扩大了生产低凝。

关键词：加氢精制加氢脱蜡低硫低凝点柴油工艺

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局部进展期胰腺癌新辅助治疗的现状与进展

中国普通外科杂志 2023年第3期32卷 317-326页

作者：柯牧京纪连栋李宜雄中南大学湘雅医院超声影像科湖南长沙410008 中南大学湘雅医院普通外科湖南长沙410008

胰腺癌恶性程度高,其总体5年生存率仅约11%。虽然根治性手术切除可能治愈胰腺癌,但仅约15%胰腺癌在首次确诊时为可切除性疾病。新辅助治疗使得有些原本不可切除的局部进展期胰腺癌(LAPC)获得了R0切除的机会。LAPC新辅助治疗是基于目前... 详细信息

胰腺癌恶性程度高,其总体5年生存率仅约11%。虽然根治性手术切除可能治愈胰腺癌,但仅约15%胰腺癌在首次确诊时为可切除性疾病。新辅助治疗使得有些原本不可切除的局部进展期胰腺癌(LAPC)获得了R0切除的机会。LAPC新辅助治疗是基于目前治疗现状的一种新的治疗模式,逐渐为临床外科医生接受。新辅助治疗方案的出现,使得20%~61%的LAPC经新辅助治疗后转化为可切除病例。奥沙利铂、伊立替康、氟尿嘧啶和亚叶酸钙(FOLFIRINOX)及吉西他滨联合白蛋白紫杉醇(AG)明显提高了LAPC的手术切除率,是LAPC首选一线新辅助治疗方案。各医疗中心关于LAPC新辅助治疗的方案选择、周期、评估指标、手术时机等方面仍存在较大差异。部分术前全身化疗不足以使肿瘤降期达到手术指征的LAPC患者,可将联合化放疗作为初始治疗。对于不能耐受系统性化疗的LAPC患者,可采用立体定向放射治疗(SBRT)控制局部肿瘤进展。胰腺癌的治疗靶点包括KRAS、EGFR、PARP及NTRK等。NCCN指南建议对所有LAPC患者进行基因检测,指导最佳药物治疗方案及参与新药的临床研究。胰腺癌免疫治疗主要包括免疫检查点抑制剂、过继性T细胞治疗及肿瘤疫苗。帕博利珠单抗是唯一获得美国食品药品管理局批准用于微卫星高度不稳定或错配修复缺陷的实体瘤(包括胰腺癌)的二线治疗方案。目前过继性T细胞治疗仅限于转移性胰腺癌。肿瘤疫苗GVAX现处于临床试验阶段。免疫治疗与某些靶向药物(如抗血管生成因子及酪氨酸激酶抑制剂)之间存在协同作用。未来的免疫治疗应旨在联合多种新型免疫治疗策略,以及联合细胞毒性药物和/或局部消融治疗,针对肿瘤诱导的免疫逃逸机制产生作用。关于联合治疗,药物选择、给药顺序及剂量将是主要挑战。选择特定的LAPC患者行新辅助治疗后实施手术是值得积极推荐的方法。通过影像学评估新辅助治疗后肿瘤的可切除性是困难的,CT无法准确区分肿瘤组织与纤维组织。18F-FDG PET判断胰腺癌R0可切除性较CT更加准确,但未来仍然需要高质量的证据进一步证实。其他对于新辅助治疗效果的评估包括肿瘤标志物血清学水平下降以及临床症状的改善。液体活检技术(包括循环肿瘤细胞、循环肿瘤DNA及外泌体检测等)在确定微转移灶及新辅助治疗疗效评估方面显现出潜在的应用前景。新辅助治疗后接受手术切除的LAPC患者长期生存率有所提高。创新技术动脉外膜鞘剥离及自体小肠移植有助于新辅助治疗后的手术切除。笔者就LAPC新辅助治疗的现状和进展等方面进行回顾和讨论。

关键词：胰腺肿瘤肿瘤辅助疗法综述

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曲靖地区电离层多参数变化特征

空间科学学报 2022年第6期42卷 1122-1128页

作者：代连东丁宗华杨嵩苗建苏许正文吴健中国电波传播研究所电波环境特性及模化技术重点实验室青岛266107 昆明电磁波环境国家野外科学观测研究站曲靖655500

利用电波环境观测网曲靖站电离层垂直探测仪数据(2008-2018),分析了该站电离层多参数(包括fmin,f_(0)E_(s),h’E_(s),f_(0)E,f_(0)F_(1),f_(0)F_(2),h’F,MUF(3000)F_(2)等)随太阳活动、季节、地方时的变化特征。结果表明,fmin,f_(0)E_(s),h’E_(s)随太阳活动变化不明显,f_(0)E,f_(0)F_(1),f_(0)F_(2),h’F,MUF(3000)F_(2)与太阳活动变化具有相关性,f_(0)F_(2)经常发生日落增强现象,同时各自具有独立性;与拉萨、乌鲁木齐地区f_(0)F_(2),h’F,f_(0)E_(s)变化特征对比,发现地方时与月份变化趋势相同,但是极值出现的位置有所不同,这可能与各地区的地理纬度和地形等因素有关;h’F夜间大于白天,在日出日落时段有突然上升现象,冬季的h’F一般都小于其他季节;MUF(3000)F_(2)与f_(0)F_(2)的变化特征相似,白天值高于夜晚值,春秋分季高于夏冬季,太阳活动高年日落后MUF(3000)F_(2)一直维持较高值并持续到03:00-04:00 LT。

关键词：曲靖电离层垂直探测仪参数特征