Oil A induces apoptosis of pancreatic cancer cells via caspase activation, redistribution of cell cycle and GADD expression

作     者：Mi-LianDong Yue-ChunZhu JohnV.Hopkins 

作者机构：AffiliatedTaizhouHospitalWenzhouMedicalCollegeLinhai317000ZhejiangProviceChina NorthwesternUniversityMedicalSchoolChicagoIL60611-3008U.S.A 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2003年第9卷第12期

页      面：2745-2750页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100214[医学-肿瘤学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：the National Cancer Institute of USA,No.CA72712 Special Funds for Zhejiang 151 Talent Project of China,No.98-2095 

主　　题：胰腺癌 细胞凋亡 血流细胞计数 载脂蛋白 GADD 抗癌作用 A油 

摘      要：AIM: To explore the mechanisms of effects of oil A on apoptosis of human pancreatic cancer ***: Cellular DNA content was analyzed by flow cytometry. Western blotting was used for caspase-3 and PARP, caspase-7, caspase-9, cytochrome c, Bcl-2, Bax, Mcl-1, cyclinA, cyclin B1, cyclin D1, cyclin E, CDK2, CDK4, CDK6,P21, P27,GADD45, ***: The caspase-3, caspase-7, and caspase-9activities were significantly increased as well as the cleavage of caspase-3, downstream substrate poly-ADP ribose polymerase (PARP) was induced. The amount of cytochrome c in the cytosolic fraction was increased, while the amount of cytochrome c in the mitochondrial fraction was decreased after oil A treatment. The anti-apoptosis proteins Bcl-2and Mci-1 were decreased in parallel and Bax increased,indicating that Bcl-2 family proteins-mitochondria-caspase cascade was responsible for oil-induced apoptosis. The proportion of cells in the G0/G1 decreased in MiaPaCa-2and AsPC-1 cells after the treatment of oil A for 24 *** number of cells in S phase was increased in two cancer cell lines at 24 hours. Therefore, cells were significantly accumulated in G2/M phase. The cells with a sub-G0/G1DNA content, a hallmark of apoptosis, were seen at 24 hours both in MiaPaCa-2 and AsPC-1 cells following exposure to oil A. The expression of cyclin A and cyclin B1 was slightly decreased and cyclin D1 levels were markedly lowered in MiaPaCa-2 cells. The expression of cyclin A and cyclin B1 was markedly decreased and cyclin D1 levels were slightly lowered in AsPC-1 cells, while cydin E was not affected and the levels of CDK2, CDK4, and CDK6 were unchanged in MiaPaCa-2 and AsPC-1 cells. In response to oil A, P21 expression was increased, but P27 expression was not affected. The expression of both GADD45 and GADD153was increased in two cell lines following oil A ***: Oil A induces apoptosis of pancreatic cancer cells via activating caspase cascade, modifying cell cycle progress