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Construction and characterization of UAA-controlled recombinant Zika virus by genetic code expansion

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Science china(life sciences) 2021年第1期64卷 171-173页

作者： Rong-Rong Zhang Qing Ye Xiao-feng li Yong-Qiang Deng yan-Peng Xu xing-Yao Huang Qing Xia cheng-feng Qin Graduate school Anhui Medical UniversityHefei230032China Department of Virology State Key Laboratory of Pathogen and BiosecurityBeijing Institute of Microbiology and EpidemiologyBeijing100071China Department of Chemical Biology State Key Laboratory of Natural and Biomimetic DrugsSchool of Pharmaceutical SciencesPeking UniversityBeijing100191China

Dear Editor,Genetic code expansion based on orthogonal tRNA-aminoacyl tRNA synthetase is an emerging technology to sitespecifically incorporate unnatural amino acids(UAAs)into viral proteins to produce UAA-controlled ... 详细信息

关键词： expansion characterization incorporate

Direct synthesis of α-hydroxyketone phosphates from terminal alkynes and H-phosphine oxides in the presence of PhI(OAc)2 and H2O

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Chinese Chemical Letters 2016年第11期27卷 1691-1695页

作者： Dong-yan Hu Meng-Shun li Wen-Wu Zhong Jian-xin Ji Jin Zhu Wei Wei Qiang Zhang Ming cheng chengdu Institute of Organic Chemistry Chinese Academy of Sciences Chengdu 610041 China chengdu Institute of Biology Chinese Academy of Sciences Chengdu 610041 China university of the Chinese Academy of sciences Beijing 100049 China school of Chemistry and Chemical Engineering Qufu Normal University Qufu 273165 China

A simple and highly efficient one-pot method for the construction of α-hydroxyketone phosphates from terminal alkynes and H-phosphine oxides has been developed in the presence of PhI（OAc）2 and H20. The present protocol provides an attractive approach to α-hydroxyketone phosphates in good to high yields, with the advantages of operation simplicity, the use of commercially available materials, broad substrate scope, high atom efficiency and good tolerance to scale-up synthesis.

关键词： α-Hydroxyketone phosphates Alkynes H-Phosphine oxides Water Phenyliodine diacetate

滴灌条件下散水介质规格对龟裂碱土水盐运移特征的影响

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土壤通报 2024年第4期55卷 944-952页

作者：黄鹏飞黄艳丽康跃虎万书勤李晓彬刘春成中国农业科学院农田灌溉研究所河南新乡453004 新乡学院土木工程与建筑学院河南新乡453003 中国科学院地理科学与资源研究所陆地水循环及地表过程重点实验室北京100101 中国科学院大学资源与环境学院北京100049 中国农业科学院农业资源与农业区划研究所北京100081

【目的】探讨滴灌改良利用龟裂碱土方法中散水介质规格对剖面水盐运移特征的影响,研究结果为散水介质规格的选择提供科学依据。【方法】通过室内土柱模拟试验,在一维和二维条件下对比分析了不同散水介质规格对龟裂碱土水盐运移特征的影... 详细信息

【目的】探讨滴灌改良利用龟裂碱土方法中散水介质规格对剖面水盐运移特征的影响,研究结果为散水介质规格的选择提供科学依据。【方法】通过室内土柱模拟试验,在一维和二维条件下对比分析了不同散水介质规格对龟裂碱土水盐运移特征的影响。【结果】一维垂直入渗条件下,平铺型散水介质的深度在0~10 cm越小或10~15 cm越大,均能获得较高的垂直湿润峰扩散速度,随散水介质深度减小,灌水频率增加,促进了盐分淋洗。二维入渗条件下,垂直湿润峰入渗速率大于水平湿润峰入渗速率,稳定入渗阶段两者均随半球型散水介质直径的增加而下降,但单位灌溉水盐分淋洗效率降低,单位时间盐分淋洗效率不变,散水介质直径≥20 cm时水分扩散速率变化趋缓。【结论】不同散水介质规格改变了灌水频率和单次灌水量,进而影响龟裂碱土的水分入渗和盐分淋洗过程。实际应用中在考虑灌溉水淋洗效率的同时要兼顾经济效益,满足一年生植物生长需求后散水介质深度越小越好,而多年生作物采用半球型散水介质时直径不易超过20 cm。

关键词：盐碱地改良龟裂碱土滴灌散水介质水盐运移

心肌缺血再灌注损伤中心肌免疫细胞单细胞图谱绘制及具有心肌保护效应的Ym-1hi中性粒细胞的鉴定

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Science Bulletin 2024年第7期69卷 949-967页

作者：董亚兰康真玉张子力张永强周海锋刘艳飞帅欣欣李君仪殷梁青青王埙埙马彦范恒 feng Jiang 林志豪丁从珠金渊真 Alexey Sarapultsev 李芳菲张戈谢天尹长军程翔罗珊珊刘玥胡德胜 Department of Integrated Traditional Chinese and Western Medicine Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Hubei Key Laboratory of Biological Targeted Therapy Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western MedicineNanjing University of Chinese MedicineNanjing 210008China National Clinical Research Center for Chinese Medicine Cardiology Xiyuan HospitalChinese Academy of Chinese Medical SciencesBeijing 100091China Department of Cardiology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of International Education Tianjin University of Traditional Chinese MedicineTianjin 300193China Institute of Neuroscience School of MedicineXiamen UniversityXiamen 361102China school of Traditional Chinese Medicine Xiamen University MalaysiaSepang 43900Malaysia school of Medical Biology South Ural State UniversityChelyabinsk 620049Russia Shum Yiu Foon Sum Bik Chuen Memorial Centre for Cancer and Inflammation Research(CCIR) School of Chinese MedicineHong Kong Baptist UniversityHong Kong 999077China Institute of Integrated Bioinfomedicine and Translational Science School of Chinese MedicineHong Kong Baptist UniversityHong Kong 999077China Division of Vascular Surgery The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Institute of Precision Medicine The First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080China Institute for Cardiovascular Prevention(IPEK) Ludwig-Maximilians-UniversityMunich 80336Germany Institute of Hematology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China china-Russia Medical Research Center for Stress Immunology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Myocardial ischemia–reperfusion injury(MIRI)is a major hindrance to the success of cardiac reperfusion *** increased neutrophil infiltration is a hallmark of MIRI,the subtypes and alterations of neutrophils in this process remain ***,we performed single-cell sequencing of cardiac CD45^(+)cells isolated from the murine myocardium subjected to MIRI at six-time *** identified diverse types of infiltrating immune cells and their dynamic changes during *** neutrophils showed the most immediate response and largest changes and featured with functionally heterogeneous subpopulations,including Ccl3^(hi)Neu and Ym-1^(hi)Neu,which were increased at 6 h and 1 d after reperfusion,***-1^(hi)Neu selectively expressed genes with protective effects and was,therefore,identified as a novel specific type of cardiac cell in the injured *** analysis indicated that neutrophils and their subtypes orchestrated subsequent immune responses in the cardiac tissues,especially instructing the response of *** abundance of Ym-1^(hi)Neu was closely correlated with the therapeutic efficacy of MIRI when neutrophils were specifically targeted by anti-Lymphocyte antigen 6 complex locus G6D(Ly6G)or anti-Intercellular cell adhesion molecule-1(ICAM-1)neutralizing *** addition,a neutrophil subtype with the same phenotype as Ym-1^(hi)Neu was detected in clinical samples and correlated with ***-1 inhibition exacerbated myocardial injury,whereas Ym-1 supplementation significantly ameliorated injury in MIRI mice,which was attributed to the tilt of Ym-1 on the polarization of macrophages toward the repair phenotype in myocardial ***,our findings reveal the antiinflammatory phenotype of Ym-1^(hi)Neu and highlight its critical role in myocardial protection during the early stages of MIRI.

关键词： Myocardial ischemia-reperfusion injury Neutrophil Ym-1 Cardiac immune response Single-cell sequencing

Electrophoretic-deposited novel ternary silk fibroin/graphene oxide/hydroxyapatite nanocomposite coatings on titanium substrate for orthopedic applications

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Frontiers of Materials Science 2016年第3期10卷 270-280页

作者： Ming li Pan XIONG Maosong MO yan cheng Yufeng ZHENG Institute of Electrical Engineering Chinese Academy of Sciences Beijing 100190 China school of Electronic Electrical and Communication Engineering University of Chinese Academy of Sciences Beijing 100049 China Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China

The combination of graphene oxide （GO） with robust mechanical property, silk fibroin （SF） with fascinating biological effects and hydroxyapatite （HA） with superior osteogenic activity is a competitive approach to make novel coatings for orthopedic applications. Herein, the feasibility of depositing ternary SF/GO/HA nanocomposite coatings on Ti substrate was firstly verified by exploiting electrophoretic nanotechnology, with SF being used as both a charging additive and a dispersion agent. The surface morphology, microstructure and composition, in vitro hemocompatibility and in vitro cytocompatibility of the resulting coatings were investigated by SEM, Raman, FTIR spectra and biocompatibiUty tests. Results demonstrated that GO, HA and SF could be co-deposited with a uniform, smooth thin-film morphology. The hemolysis rate analysis and the platelet adhesion test indicated good blood compatibility of the coatings. The human osteosarcoma MG63 cells displayed well adhesion and proliferation behaviors on the prepared coatings, with enhanced ALP activities. The present study suggested that SFIGO/HA nanocomposite coatings could be a promising candidate for the surface functionalization of biomaterials, especially as orthopedic implant coating.

关键词： graphene oxide silk fibroin hydroxyapatite nano-biocomposite coatings electrodeposition

Generating wind power time series based on its persistence and variation characteristics

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Science china(Technological sciences) 2014年第12期57卷 2475-2486页

作者： li Jing Hua li Jia Ming WEN Jin Yu cheng Shi Jie XIE Hai lian YUE cheng yan china State Key Laboratory of Advanced Electromagnetic Engineering and Technology Huazhong University of Science and TechnologySchool of Electrical and Electronic Engineering school of Electrical Engineering Guangxi University ABB (china) Ltd. Corporate Research Center

Generation of wind power time series is an important foundational task for assisting electric power system planning and mak- ing decision. By analyzing the characteristics of wind power persistence and variation, th!.s paper proposes an improved Mar- kov chain Monte Carlo （MCMC） method, identified as the PV-MC method, for the direct generation of a synthetic series of wind power output. On the basis of the MCMC method, duration time and variation features are concluded in PV-MC method, gaining a more comprehensive reflection of wind power characteristics in the generated wind power time series. First, the wind power state series is generated to meet the state transition matrix based on the definition of the wind power state. Then, the time duration of each state in the series is determined by its respective duration character. Finally, the variation characteristic is used to convert the state series to a wind power time series. A significant amount of simulations are performed based on the PV-MC and MCMC methods and are then compared for 25 wind farms at 6 different locations throughout the world. The sim- ulation results show that the PV-MC method offers an excellent fit for the time domain features （persistence and variation characteristic） while holding other statistic features （mean value, variance, autocorrelation coefficient （ACC） and probability density function （PDF）） close to the MCMC method.

关键词： wind power time series generation persistence characteristic variation characteristic markov chain monte carlomethod

1960—2023年我国东北典型季节性冻土区冻融指数及冻土退化影响因素分析

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水利水电技术（中英文） 2024年第8期55卷 161-173页

作者：李保琦张楚楚周毓彦丁红吕航常诚燕玉亮石家庄铁道大学土木工程学院河北石家庄050043 中国水利水电科学研究院流域水循环模拟与调控国家重点实验室北京100038 黑龙江省水利科学研究院黑龙江哈尔滨150000 吉林大学吉林长春130012 松辽水利委员会松辽流域水土保持监测中心站吉林长春130000 黑龙江大学水利电力学院黑龙江哈尔滨150080

【目的】季节性冻土的冻融循环过程显著影响了流域水循环和冻土层的演变。明晰冻融过程演变规律,为保障季节性冻土区生态及水利水电工程的建设和运行管理提供理论支撑。【方法】基于我国东北部典型季节性冻土区的10个气象站和冻土观测... 详细信息

【目的】季节性冻土的冻融循环过程显著影响了流域水循环和冻土层的演变。明晰冻融过程演变规律,为保障季节性冻土区生态及水利水电工程的建设和运行管理提供理论支撑。【方法】基于我国东北部典型季节性冻土区的10个气象站和冻土观测站数据,分析1960—2020年冻融指数的时空分布特征,计算了最大冻结深度、冻结开始日期、完全融化日期、冻融期、冻土退化速率,并结合气候(年平均气温、冻结温度变化率、冻结指数、融化指数)及地理参数(经纬度、海拔),利用相关性分析评估1960—2023年典型季节性冻土区最大冻结深度、冻土退化速率与冻融状态的影响。【结果】我国东北部典型季节性冻土区冻结指数以55.10℃·d/10 a的速率减小,融化指数以60.80℃·d/10 a的速率增加。60 a间最大冻结深度范围为68.00~260.00 cm,冻土退化速率范围为0.07~1.45 cm/a,开始冻结日期推迟速率为1.15 d/10 a,完全融化日期以4.71 d/10 a的速率显著提前,冻融期以5.60 d/10 a的速率缩短。【结论】冻结指数与纬度的相关性大,而融化指数与海拔相关性强。我国东北部典型季节性冻土区最大冻结深度主要受年平均气温和纬度影响,冻土退化主要受冻结温度变化率影响,冻融期显著受年平均气温影响。

关键词：冻融特征时空演变影响因素贡献分解季节性冻土区

Effective and Robust Detection of Adversarial Examples via Benford-Fourier Coefficients

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Machine Intelligence Research 2022年

作者： cheng-cheng Ma Bao-Yuan Wu yan-Bo Fan Yong Zhang Zhi-feng li Shenzhen Research Institute of Big Data National Laboratory of Pattern Recognition Institute of Automation Chinese Academy of Sciences school of Data Science The Chinese University of Hong Kong school of Artificial Intelligence University of Chinese Academy of Sciences AI Lab Tencent Inc.

Adversarial example has been well known as a serious threat to deep neural networks(DNNs). In this work, we study the detection of adversarial examples based on the assumption that the output and internal responses of one DNN model for both adversarial and benign examples follow the generalized Gaussian distribution(GGD) but with different parameters(i.e., shape factor, mean, and variance). GGD is a general distribution family that covers many popular distributions(e.g., Laplacian, Gaussian, or uniform). Therefore, it is more likely to approximate the intrinsic distributions of internal responses than any specific distribution. Besides, since the shape factor is more robust to different databases rather than the other two parameters, we propose to construct discriminative features via the shape factor for adversarial detection, employing the magnitude of Benford-Fourier(MBF) coefficients, which can be easily estimated using responses. Finally, a support vector machine is trained as an adversarial detector leveraging the MBF features. Extensive experiments in terms of image classification demonstrate that the proposed detector is much more effective and robust in detecting adversarial examples of different crafting methods and sources compared to state-of-the-art adversarial detection methods.

关键词：

ligustrazine monomer against cerebral ischemia/reperfusion injury

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Neural Regeneration Research 2015年第5期10卷 832-840页

作者： Hai-jun Gao Peng-fei liu Pei-wen li Zhuo-yan Huang feng-bo Yu Ting Lei Yong Chen Ye cheng Qing-chun Mu Hai-yan Huang Department of Neurosurgery First Bethune Hospital of Jilin University Department of Neurosurgery Hongqi Hospital of Mudanjiang Medical University Clinical Medical College of Beihua university school of Pharmacy Mudanjiang Medical University

ligustrazine （2,3,5,6-tetramethylpyrazine） is a major active ingredient of the Szechwan lovage rhizome and is extensively used in treatment of ischemic cerebrovascular disease. The mecha- nism of action of ligustrazine use against ischemic cerebrovascular diseases remains unclear at present. This study summarizes its protective effect, the optimum time window of administra- tion, and the most effective mode of administration for clinical treatment of cerebral ischemia/ reperfusion injury. We examine the effects of ligustrazine on suppressing excitatory amino acid release, promoting migration, differentiation and proliferation of endogenous neural stem cells. We also looked at its effects on angiogenesis and how it inhibits thrombosis, the inflammatory response, and apoptosis after cerebral ischemia. We consider that ligustrazine gives noticeable protection from cerebral ischemia/reperfusion injury. The time window of ligustrazine admin- istration is limited. The protective effect and time window of a series of derivative monomers of ligustrazine such as 2-[（1,1-dimethylethyl）oxidoimino]methyl]-3,5,6-trimethylpyrazine, CXC137 and CXC 195 after cerebral ischemia were better than ligustrazine.

关键词： nerve regeneration ligustrazine ischemia cerebral ischemia/reperfusion injury neuralregeneration