Electrophoretic-deposited novel ternary silk fibroin/graphene oxide/hydroxyapatite nanocomposite coatings on titanium substrate for orthopedic applications

作     者：Ming LI Pan XIONG Maosong MO Yan CHENG Yufeng ZHENG 无

作者机构：Institute of Electrical Engineering Chinese Academy of Sciences Beijing 100190 China School of Electronic Electrical and Communication Engineering University of Chinese Academy of Sciences Beijing 100049 China Center for Biomedical Materials and Tissue Engineering Academy for Advanced Interdisciplinary Studies Peking University Beijing 100871 China Department of Materials Science and Engineering College of Engineering Peking University Beijing 100871 China 

出 版 物：《Frontiers of Materials Science》 (材料学前沿（英文版）)

年 卷 期：2016年第10卷第3期

页      面：270-280页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0703[理学-化学] 070301[理学-无机化学] 

基　　金：This work was supported by the Beijing Natural Science Foundation (Grant No. 2164073)  the National Natural Science Foundation of China (Grant Nos. 21271032  31370954 and 51431002)  the Scientific and Technical Program of Beijing (Grant No. Z141100002814008)  and the Innovative-Talent Program (Institute of Electrical Engineering  Chinese Academy of Sciences) 

主　　题：graphene oxide silk fibroin hydroxyapatite nano-biocomposite coatings electrodeposition 

摘      要：The combination of graphene oxide （GO） with robust mechanical property, silk fibroin （SF） with fascinating biological effects and hydroxyapatite （HA） with superior osteogenic activity is a competitive approach to make novel coatings for orthopedic applications. Herein, the feasibility of depositing ternary SF/GO/HA nanocomposite coatings on Ti substrate was firstly verified by exploiting electrophoretic nanotechnology, with SF being used as both a charging additive and a dispersion agent. The surface morphology, microstructure and composition, in vitro hemocompatibility and in vitro cytocompatibility of the resulting coatings were investigated by SEM, Raman, FTIR spectra and biocompatibiUty tests. Results demonstrated that GO, HA and SF could be co-deposited with a uniform, smooth thin-film morphology. The hemolysis rate analysis and the platelet adhesion test indicated good blood compatibility of the coatings. The human osteosarcoma MG63 cells displayed well adhesion and proliferation behaviors on the prepared coatings, with enhanced ALP activities. The present study suggested that SFIGO/HA nanocomposite coatings could be a promising candidate for the surface functionalization of biomaterials, especially as orthopedic implant coating.