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Association Between VDR FokI Polymorphism and Intervertebral Disk Degeneration

Genomics, Proteomics & Bioinformatics 2015年第6期13卷 371-376页

作者： Jian Zhao mingyuan Yang Jie Shao Yushu Bai ming li Department of Orthopaedics Changhai Hospital Second Military Medical University

Intervertebral disk degeneration （IDD） is strongly associated with genetic predisposition and environmental susceptibility. Several studies been conducted to investigate the potential asso- ciation between IDD and FokI polymorphism located in the gene encoding the vitamin D receptor （VDR）, and inconsistent conclusions had been reached among different ethnic populations. In order to assess the association between the FokI polymorphism and the risk of IDD, we performed a comprehensive and systematic meta-analysis. Candidate articles were retrieved from PubMed, EMBASE, China National Knowledge Infrastructure （CNKI）, and China Biology Medical （CBM） with strict inclusion criteria in January 2015. Among the 54 articles that were retrieved, only eight studies met the inclusion criteria. The pooled data analysis based on allele contrast, homozy- gore, heterozygote, dominant, and recessive models revealed no significant correlation between the FokI polymorphism and the risk of IDD. However, when stratified by ethnicity, significant associ- ations were detected for Hispanics based on allele contrast （OR = 1.395, 95% CI = 1.059-1.836, P = 0.018）, homozygote （OR = 1.849, 95% CI = 1.001-3.416, P = 0.049）, heterozygote （OR = 1.254, 95% CI = 1.049-1.498, P = 0.013）, and dominant （OR = 1.742, 95% CI = 1.174-2.583, P = 0.006） models, and for Asians using the dominant model （OR = 1.293, 95% CI = 1.025-1.632, P --- 0.030）, whereas there is no significant association detected for Caucasians. In conclusion, FokI polymorpbism is not generally associated with IDD, but there is increased risk for IDD in Hispanics and Asians carrying FokI allele T.

关键词： FokI polymorphism rs2228570 Vitamin D receptor Disk degeneration Intervertebral disk disease

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Microemulsion-mediated hydrothermal synthesis of flower- like MoS2 nanomaterials with enhanced catalytic activities for anthracene hydrogenation

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Frontiers of Chemical Science and Engineering 2018年第1期12卷 32-42页

作者： Yuxia Jiang Donge Wang Zhendong Pan Huaijun ma Min li Jiahe li Anda Zheng Guang Lv Zhijian Tian Dalian National Laboratory for Clean Energy Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China University of Chinese Academy of Sciences Beijing 100049 China State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China

Flower-like intercalated MoS2 nanomaterials have been successfully synthesized via a microemulsion- mediated hydrotherrnal （MMH） method, and characterized by X-ray diffraction, Raman spectroscopy, element analysis, scanning electron microscopy, transmission electron microscopy, thermogravimetric analysis, and Fourier transform infrared spectroscopy in detail. Their catalytic performance for anthracene hydrogenation was evaluated using a slurry-bed batch reactor with an initial hydrogen pressure of 80 bar at 350 ℃ for 4 h. The intercalated MoS2 nanoflowers synthesized from Na2MoO4 （MoS2-S） and H2MoO4 （MoS2-A） as molybde- num precursors have diameters of about 150 and 50 nm, respectively. MoS2 nanosheets on MoS2-S and MoS2-A possess stacking layer numbers of 5-10 and 2-5, and slab lengths of about 15 and 10 nm, respectively. The interlayer distances of MoS2-S and MoS2-A are both enlarged from 0.62 nm to about 0.95 nm due to the intercalation ofNH4＋ and surfactant molecules. The MoS2 nanoflowers have high catalytic activities for anthracene hydrogenation. The selectivity for octahydroanthracene, a deeply hydrogenated product, over MoS2-A is 89.8%, which is 31.0 times higher than that over commercial bulk MoS2. Fully hydrogenated product （perhydroanthracene） was also detected over MoS2 nanoflowers with a selectivity of 3.7%. The enhanced hydrogenation activities of MoS2 nanoflowers can be ascribed to the high exposure of catalytic active sites, resulting from the smaller particle size, fewer stacking layer, shorter slab length and enlarged interlayer distance of MoS2 nanoflowers compared with commercial bulk MoS2. In addition, a possible growth mechanism of MoS2 nanoflowers synthesized via the MMH method was proposed.

关键词： microemulsion intercalated MoS2 catalytic hydrogenation active sites

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A New Pseudo Sesquiterpenoid from the Seeds of Koelreuteria paniculata

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Chinese Chemical Letters 2002年第11期13卷 1067-1068页

作者： Wen Han liN Hai ming LEI Hong Zheng FU Jun li National Research Laboratories of Natural and Biomimetic Drugs Peking UniversityBeijing 100083

From the seeds of medicinal plant Koelreuteria paniculata, a new pseudo sesquiter- penoid with novel skeleton namely paniculoid 1 was isolated. The structure of 1 was established on the basis of extensive 2D NMR spec... 详细信息

关键词： Koelreuteria Paniculata seeds paniculoid structural elucidation.

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A novel Lactococcus lactis L-arabinose isomerase for D-tagatose production from lactose

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Food Bioscience 2022年第4期48卷 339-348页

作者： Susu Zhang Zhenshang Xu ming ma Guoyan Zhao Runlei Chang Hongli Si Meixue Dai College of life Science Shandong Normal UniversityJinan250014PR China State Key Laboratory of Biobased material and Green Papermaking Qilu University of TechnologyShandong Academy of ScienceJinan250353PR China Shandong Provincial Key Laboratory of Microbial Engineering Department of BioengineeringQilu University of TechnologyShandong Academy of ScienceJinan250353PR China

A new strain of Lactococcus lactis producing D-tagatose was isolated and *** L-arabinose isomerase coding gene was cloned and expressed in Escherichia coli BL21(DE3).The optimal temperature and pH of the purified enzyme were 50◦C and *** produce D-tagatose from lactose,β-D-galactosidases from ***,Lactiplantibacillus plantarum,and Streptococcus thermophilus were further incorporated into *** by two ***β-D-galactosidases were fused to L-arabinose isomerase coupled with a peptide linker(GGGGS)***,they were co-expressed with L-arabinose isomerase using pETDuet-1 *** these recombinant strains,the cell co-expressing L-arabinose isomerase and ***β-D-galactosidase showed maximal ***-PAGE results confirmed that exogenous enzymes had the maximum soluble expression level in this *** the optimal condition,the conversion rate of D-tagatose from 300 g/L lactose achieved to 42.4%,and the volumetric productivity reached 4.28 g/L/h at 15 *** research established a highly efficient biotransformation system to produce D-tagatose from lactose.

关键词： Lactose D-tagatose Lactic acid bacteria L-arabinose isomerase β-D-galactosidase

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HYDRODYNAMIC INTERACTIONS BETWEEN TWO BODIES

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Journal of Hydrodynamics 2007年第6期19卷 784-785页

作者： CHENG li Wu De-ming Xu Yu-ru Wang Yan-ying li Ji-de Zhang liang Ding Yong College of Shipbuilding Engineering Harbin Engineering University Harbin 150001 China Harbin Engineering University （member of CAE Harbin Engineering University） Chairman Dalian University of Technology

On the basis of model tests, potential flow theory, and viscous Computational Fluid Dynamics （CFD） method, the hydrodynamic interactions between two underwater bodies were investigated to determine the influencing factors, changing rule, interaction mechanism, and appropriate methods describing them. Some special phenomena were discovered in two series of near-wall interaction experiments. The mathematical model and predicting methods were presented for interacting forces near wall, and the calculation results agreed well with the experimental ones. From the comparisons among numerical results with respect to nonviscosity, numerical results with respect to viscosity, and measured results, data on the influence of viscosity on hydrodynamic interactions were obtained. For hydrodynamic interaction related to multi-body unsteady motions with six degrees of freedom that is difficult to simulate in tests, numerical predictions of unsteady interacting forces were given.

关键词： hydrodynamic interactions UUV unsteady flow wall effect panel method model test Kutta condition

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Preparation and properties of two new soluble carbazole-containing functional polyacetylenes

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Chinese Chemical Letters 2011年第9期22卷 1017-1020页

作者： Wei Ju Zhu Zhen Yu Wu Ji ming Song Cun li School of Chemistry and Chemical Engineering Anhui University Hefei 230039 China

Two new functional polyacetylenes bearing carbazole group as pendant, poly { 3-[（4-ethynylstyryl）-N-butyl] carbazole } （P1 ） and poly{3-14-（prop-2-ynyloxy）phenyl-N-butyl]carbazole}（P2）, were prepared using [Rb（nbd）Cl]2-Et3N as catalyst. The polymers were soluble in common organic solvents such as CHCl3 and THF. Their structures and properties were characterized and evaluated with FTIR, lit NMR, UV, TGA, GPC, and CV, respectively. The results show that the polymers possess high thermal stability and well hole-injection property.

关键词： Polyacetylene CV Hole-injection Carbazole Thermal stability

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Analysis of Specific Binding and Subcellular Localization of Wheat ERF Transcription Factor W17

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Agricultural Sciences in China 2008年第6期7卷 647-655页

作者： ZHAO Yun-xiang liU Pei XU Zhao-shi CHEN ming li lian-cheng CHEN Yao-feng XIONG Xiang-jin ma You-zhi Institute of Crop Science Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement （NFCR1）/Key Laboratory of Crop Genetics and Breeding Ministry of Agriculture Beijing 100081 P.R.China Agronomy College Northwest A ＆ F University Yangling 712100 P.R.China

The study aims to detect the subcellular localization of ERF （ethylene-responsive element binding factor） transcription factor W17 protein, the interaction between W 17 and cis-acting regulatory elements GCC-box and DRE in vitro, the binding and transactivating ability in vivo, and the role of W17 in higher plant stress-signal pathway. Recombinant plasmid W17/163hGFP was introduced into onion epidermal cells by the particle bombardment method with a PDS 1000/He. Transformed cells were incubated for 24 h at 22℃ in the dark and green fluorescence was monitored under a confocal microscope. The gene W17 was fused N-terminus of GST （glutathione-S-transferase） in prokaryotic expression vector pGEX-4T-1 and then transformed into E. coli strain BL21 （DE3）. IPTG （0.5 mmol L-1） was added to induce the expression of recombinant GST/W17 for 3 h. The fused proteins were purified by GST purification columns, and then subjected to gel retardation assay with a 32p-labeled GCC or DRE sequence. The different reporter and effector plasmids were introduced into tobacco leaves through agroinfiltration, then transformed leaves stained by X-Gluc, faded with 75% alcohol and monitored under a Stereozooming microscope. The GFP fused with W17 protein was localized in the nuclei; SDS-PAGE assay demonstrated that the fused protein GST/W17 could be induced and purified with molecular weight at around 42.2 kD under the induction of IPTG. Purified fused protein was able to specifically bind to both the wild-type GCC-box and DRE element, but had no interaction with either the mutant DRE or GCC-box; W17 protein can bind to GCC-box and transactive downstream GUS gene in vivo. W17 can localize into the nuclei, and it may be involved not only in biotic stresses controlled by GCC-box, but also in abiotic stresses （e.g., salt-） induced signaling pathway.

关键词： ERF/AP2 domain ERF DRE element GCC-box subcellular localization

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Review:Mitochondrial functions on oocytes and preimplantation embryos

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第7期10卷 483-492页

作者： li-ya WANG Da-hui WANG Xiang-yang ZOU Chen-ming XU Department of Biotechnology Dalian Medical University Dalian 116044 China The Sperm Laboratory Institute of Cell Biology and Genetics College of Life Sciences Zhejiang University Hangzhou 310058 China Department of Reproductive Endocrinology Women's Hospital School of Medicine Zhejiang University Hangzhou 310006 China

Oocyte quality has long been considered as a main limiting factor for in vitro fertilization （IVF）. In the past decade, extensive observations demonstrated that the mitochondrion plays a vital role in the oocyte cytoplasm, for it can provide adenosine triphosphate （ATP） for fertilization and preimplantation embryo development and also act as stores of intracellular calcium and proapoptotic factors. During the oocyte maturation, mitochondria are characterized by distinct changes of their distribution pattern from being homogeneous to heterogeneous, which is correlated with the cumulus apoptosis. Oocyte quality decreases with the increasing maternal age. Recent studies have shown that low quality oocytes have some age-related dysfunctions, which include the decrease in mitochondrial membrane potential, increase of mitochondrial DNA （mtDNA） damages, chromosomal aneuploidies, the incidence of apoptosis, and changes in mitochoncLrial gene expression. All these dysfunctions may cause a high level of de- velopmental retardation and arrest of preimplantation embryos. It has been suggested that these mitochondrial changes may arise from excessive reactive oxygen species （ROS） that is closely associated with the oxidative energy production or calcium overload, which may trigger permeability transition pore opening and subsequent apoptosis. Therefore, mitochondria can be seen as signs for oocyte quality evaluation, and it is possible that the oocyte quality can be improved by enhancing the physical function of mitochondria. Here we reviewed recent advances in mitochondrial functions on oocytes.

关键词： Mitochondria Oocyte Preimplantation embryo

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The rhizospheric microbiome becomes more diverse with maize domestication and genetic improvement

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Journal of Integrative Agriculture 2022年第4期21卷 1188-1202页

作者： HUANG Jun li Yun-feng ma Yuan-ying li Yan-sheng JIN Jian liAN Teng-xiang Key Laboratory of Plant Molecular Breeding of Guangdong Province College of AgricultureSouth China Agricultural UniversityGuangzhou 510640P.R.China Centre for Mined Land Rehabilitation Sustainable Minerals InstituteThe University of QueenslandBrisbane Qld 4072Australia Key Laboratory of Mollisols Agroecology Northeast Institute of Geography and AgroecologyChinese Academy of SciencesHarbin 150081P.R.China Department of Animal Plant and Soil SciencesCentre for AgriBioscienceLa Trobe UniversityMelbourne CampusBundoora Vic 3086Australia

Domestication and genetic improvement of maize improve yield and stress tolerance due to changes in morphological and physiological properties, which likely alter rhizosphere microbial diversity. Understanding how the evolution of maize germplasm impacts its rhizobacterial traits during the growth stage is important for optimizing plant-microbe associations and obtaining yield gain in domesticated germplasms. In this study, a total of nine accessions representing domestication and subsequent genetic improvement were selected. We then sequenced the plant DNA and rhizobacterial DNA of teosinte, landraces and inbred lines at the seedling, flowering and maturity stages in a field trial. Moreover, the soil chemical properties were determined at the respective stages to explore the associations of soil characteristics with bacterial community structures. The results showed that domestication and genetic improvement increased the rhizobacterial diversity and substantially altered the rhizobacterial community composition. The core microbiome in the rhizosphere differed among germplasm groups. The co-occurrence network analysis demonstrated that the modularity in the bacterial network of the inbred lines was greater than those of teosinte and the landraces. In conclusion, the increased diversity of the rhizobacterial community with domestication and genetic improvement may improve maize resilience to biotic stresses and soil nutrient availability to plants.

关键词： teosinte landraces inbred lines domestication and improvement core microbiome network

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Long-term outcomes of uveitic glaucoma treated with Ahmed valve implant in a series of Chinese patients

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International Journal of Ophthalmology(English edition) 2018年第4期11卷 629-634页

作者： Ning Bao Zheng-Xuan Jiang Paul Coh li-ming Tao Department of Ophthalmology the Second Affiliated Hospital of Anhui Medical University Department of Ophthalmology University of CaliforniaSan Francisco

AIM： To report long-term outcomes of secondary glaucoma due to uveitis treated with Ahmed glaucoma valve（AGV） implantation in a series of Chinese patients. METHODS： The retrospective study included 67 eyes from 56 patients with uveitic glaucoma who underwent AGV implantation. Success of the treatment was defined as patients achieving intraocular pressure（IOP） levels between 6 and 21 mm Hg with or without additional antiglaucoma medications and/or a minimum of 20% reduction from baseline IOP. The main outcome measurements included IOP, the number of glaucoma medications at 1, 3, 6, 12, 24, 36, 48 and 60 mo after surgery, surgical complications, final best-corrected vision acuity（BCVA）, visual field（VF） and retinal nerve fiber layer（RNFL）.RESULTS： The mean follow-up was 53.3±8.5（range 48 to 60）mo. The cumulative probability of success rate was 98.5%, 95.5%, 89.6%, 83.6%, 76.1%, 70.1%, 65.7% and 61.2% at 1, 3, 6, 12, 24, 36, 48 and 60 mo, respectively. IOP was reduced from a baseline of 30.8±6.8 to 9.9±4.1, 10.1±4.2, 10.9±3.7, 12.9±4.6, 13.8±3.9, 13.2±4.6, 12.3±3.5 and 13.1±3.7 mm Hg at 1, 3, 6, 12, 24, 36, 48 and 60 mo, respectively（P〈0.01）. The number of postoperative glaucoma medications was significantly decreased compared with baseline at all time points during the study period（P〈0.05）. There was no significant difference between preoperative and postoperative BCVA. Remarkable surgical complications were not found after surgery. The VF and RNFL of the patients were stable after the ***： AGV implantation is safe and effect in terms of reducing IOP, decreasing the number of glaucoma medications, and preserving vision for patients with uveitic glaucoma.

关键词： uveitic glaucoma Ahmed glaucoma valve follow-up Chinese population

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