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二甲双胍激活Hippo信号通路抑制胰腺癌细胞生长的作用

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临床和实验医学杂志 2024年第18期23卷 1905-1908页

作者：李世艳孙永辉余鸿新疆医科大学第一附属医院胰腺外科新疆乌鲁木齐830054

目的分析二甲双胍激活Hippo信号通路对胰腺癌细胞生长的抑制作用。方法采用中国科学院上海生命科学院细胞资源中心提供的人胰腺癌细胞株PANC-1,进行细胞培养,提取细胞Total RNA,测定RNA样本纯度与浓度,RNA逆转录为cDNA。实时荧光定量R... 详细信息

目的分析二甲双胍激活Hippo信号通路对胰腺癌细胞生长的抑制作用。方法采用中国科学院上海生命科学院细胞资源中心提供的人胰腺癌细胞株PANC-1,进行细胞培养,提取细胞Total RNA,测定RNA样本纯度与浓度,RNA逆转录为cDNA。实时荧光定量Real-time PCR提取细胞总蛋白,采用BCA染色法测定蛋白浓度并进行蛋白变性,蛋白质印迹法检测目的蛋白表达,分析蛋白RNA样品质检情况。二甲双胍组采用二甲双胍处理,对照组不采用二甲双胍处理,统计分析二甲双胍对人约克同源(YAP1)、大肿瘤抑制基因1(LATS1)、育系20样激酶1(MST1)mRNA表达的调节作用,并统计分析二甲双胍对P-YAP1、LATS1、MST1蛋白质表达的影响。结果所有样品质检均合格,能够进行后续检测。二甲双胍组PANC-1细胞中YAP1、LATS1、MST1 mRNA表达分别为4.16±0.36、3.72±0.85、4.04±0.47,均明显高于对照组(1.02±0.10、0.94±0.04、1.01±0.08),差异均有统计学意义(P0.05)。结论二甲双胍治疗胰腺癌能够激活Hippo信号通路,从而抑制细胞生长,发挥抗肿瘤作用。

关键词：胰腺肿瘤二甲双胍 Hippo信号通路细胞生长

AMPHIPHIliC STAR-BLOCK COPOLYMERS BY IODIDE-MEDIATED RADICAL POLYMERIZATION

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Chinese Journal of Polymer Science 2007年第6期25卷 609-619页

作者： Bing-yi li yan shi Zhi-feng Fu Wan-tai yang Shu-ke Jiao State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Beijing 100029 China

Amphiphilic star-block copolymers composed of polystyrene and poly（acrylic acid） were synthesized by iodide- mediated radical polymerization. Firstly, free radical polymerization of styrene was carried out with AIBN as initiator and 1,1,1-trimethyolpropane tri（2-iodoisobutyrate） as chain transfer agent, giving iodine atom ended star-shaped polystyrene with three arm chains, R（polystyrene）3. Secondly, tert-butyl acrylate was polymerization using polystyrene obtained as macro-chain transfer agent, and star-block copolymer, R（polystyrene-b-poly（tert-butyl acrylate））3 with controlled molecular weight was obtained. Finally, amphiphilic star-block copolymer, R（polystyrene-b-poly（acrylic acid））3 was obtained by hydrolysis of R（polystyrene-b-poly（tert-butyl acrylate））3 under acidic condition.

关键词： living radical polymerization Iodide-Mediated radical polymerization Star-block copolymers Amphiphilic copolymers.

中医辨证联合耳穴压豆对妊娠期糖尿病患者血糖及妊娠结局的影响

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中国现代药物应用 2024年第18期18卷 144-147页

作者：李世言郝银平于源源青岛大学附属威海市立第二医院(威海市妇幼保健院) 264200

目的研究妊娠期糖尿病患者给予中医辨证联合耳穴压豆治疗的效果,并观察对其血糖及妊娠结局的影响。方法遴选200例妊娠期糖尿病患者,根据电脑随机法分成对照组(98例)和治疗组(102例)。对照组给予运动、营养指导治疗,治疗组在对照组基... 详细信息

目的研究妊娠期糖尿病患者给予中医辨证联合耳穴压豆治疗的效果,并观察对其血糖及妊娠结局的影响。方法遴选200例妊娠期糖尿病患者,根据电脑随机法分成对照组(98例)和治疗组(102例)。对照组给予运动、营养指导治疗,治疗组在对照组基础上给予中医辨证联合耳穴压豆治疗。比较两组治疗前后血糖指标水平、胰岛功能、脂代谢指标水平、妊娠结局及治疗效果。结果治疗组治疗后糖化血红蛋白(5.50±0.26)%、空腹血糖(5.38±0.71)mmol/L、餐后2 h血糖(7.62±0.79)mmol/L显著低于对照组的(6.34±0.30)%、(5.96±0.87)mmol/L、(8.40±1.03)mmol/L(P<0.05)。治疗组治疗后胰岛β细胞分泌指数(1.91±0.13)、胰岛素抵抗指数(2.74±0.60)显著低于对照组的(2.25±0.10)、(3.07±0.87)(P<0.05)。治疗组治疗后甘油三酯(1.32±0.25)mmol/L、总胆固醇(5.95±0.72)mmol/L显著低于对照组的(1.76±0.31)、(6.83±0.90)mmol/L(P<0.05)。治疗组治疗总有效率91.18%显著高于对照组的78.57%(P<0.05)。治疗组自然分娩率81.37%、5 min新生儿阿氏评分(8.56±0.32)分显著高于对照组的68.37%、(6.39±0.27)分(P<0.05)。结论妊娠期糖尿病患者给予中医辨证联合耳穴压豆治疗效果显著,能够改善患者血糖及脂代谢异常,优化胰岛功能,对妊娠结局有着积极影响。

关键词：中医辨证耳穴压豆妊娠期糖尿病血糖水平妊娠结局

Influence of rare earth oxides on microstructure and mechanical properties of nickel-based superalloys fabricated by high energy beam processing:A review

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Journal of Rare Earths 2024年第9期42卷 1629-1645,I0001页

作者： lianjie Bi Hua yan Peilei Zhang Haichuan shi Zhiyuan li Ruidi li School of Materials Science and Engineering Shanghai University of Engineering ScienceShanghai201620China College of Agricultural Science and Engineering Hohai UniversityNanjing211100China Department of Mechanics and Maritime Science Chalmers University of TechnologyGothenburSE41296Sweden State Key Laboratory of Powder Metallurgy Central South UniversityChangsha410083China

In this paper,the effects of rare earth oxides on the micro structure and mechanical properties of nickelbased superalloys prepared by high-energy beam processing technology were critically *** focus is on the optimal amount of rare earth oxides that can produce ideal *** attention was paid to their main strengthening mechanisms,including solid solution strengthening mainly in the form of solid solution dissolved in the nickel-based alloy and improving the microstructure of the alloy by grain refinement or fine grain strengthening produced by homogenizing the distribution phase.Y_(2)O_(3),La_(2)O_(3) and CeO_(2) rare earth oxides can also improve the fluidity of the alloy molten pool and reduce the segregation of alloying *** advantages can significantly improve the mechanical properties of the ***,this paper outlines the future research directions of rare earth oxides,aiming to expand their application potential.

关键词： Rare earths High energy beam Nickel-based superalloys Microstructure Mechanical properties

Pd-Catalyzed Highly Regioselective Hydroesterification of Terminal Alkyl Olefins with Formates

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Chinese Journal of Chemistry 2024年第12期42卷 1381-1386页

作者： Junhua li Tianci Shen yan yang Yian shi Jiangsu Key Laboratory of Advanced Catalytic Materials&Technology Schoolof Petrochemical EngineeringInstitute of Natural and Synthetic Organic ChemistryChangzhou UniversityChangzhouJiangsu 213164China

Comprehensive Summary A Pd-catalyzed regioselective hydroesterification of alkyl terminal olefins with aryl formates is described.A wide variety of linear carboxylic esters bearing various functional groups can be obtained in good yields with high regioselectivities under mild reaction conditions by using 1,2-DTBPMB or(p-F-Ph)3P as *** reaction process is operationally simple and requires no handling of toxic CO or strong *** resulting aryl esters can be readily converted to other carboxylic acid derivatives.

关键词： Alkenes Regioselectivity Carbonylation Hydroesterification Formates

High-speed penetration of ogive-nose projectiles into thick concrete targets:Tests and a projectile nose evolution model

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Defence Technology（防务技术） 2024年第2期32卷 553-571页

作者： Xu li yan liu Junbo yan Zhenqing shi Hongfu Wang Yingliang Xu Fenglei Huang State Key Laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijing100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing401120China China Research and Development Academy of Machinery Equipment Beijing100089China

The majority of the projectiles used in the hypersonic penetration study are solid flat-nosed cylindrical projectiles with a diameter of less than 20 *** study aims to fill the gap in the experimental and analytical study of the evolution of the nose shape of larger hollow projectiles under hypersonic *** the hypersonic penetration test,eight ogive-nose AerMet100 steel projectiles with a diameter of 40 mm were launched to hit concrete targets with impact velocities that ranged from 1351 to 1877 m/*** erosion of the projectiles was observed during high-speed penetration of heterogeneous targets,and apparent localized mushrooming occurred in the front nose of recovered *** examining the damage to projectiles,a linear relationship was found between the relative length reduction rate and the initial kinetic energy of projectiles in different penetration ***,microscopic analysis revealed the forming mechanism of the localized mushrooming phenomenon for eroding penetration,i.e.,material spall erosion abrasion mechanism,material flow and redistribution abrasion mechanism and localized radial upsetting deformation ***,a model of highspeed penetration that included erosion was established on the basis of a model of the evolution of the projectile nose that considers radial upsetting;the model was validated by test data from the literature and the present *** upon the impact velocity,v0,the projectile nose may behave as undistorted,radially distorted or *** to the effects of abrasion of the projectile and enhancement of radial upsetting on the duration and amplitude of the secondary rising segment in the pulse shape of projectile deceleration,the predicted DOP had an upper limit.

关键词： High-speed penetration Concrete target Erosion Projectile nose evolution model

Triclosan-conjugated,lipase-responsive Polymeric Micelles for Eradication of Staphylococcal Biofilms

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Chinese Journal of Polymer Science 2024年第6期42卷 718-728页

作者： yan-Qiang Huang Yuan-Feng li Yong liu lin-Qi shi Guangxi Technology Innovation Cooperation Base of Prevention and Control Pathogenic Microbes with Drug Resistance Youjiang Medical University for NationalitiesBaise533000China Translational Medicine Laboratory the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou325035China Wenzhou Institute University of Chinese Academy of SciencesWenzhou325001China State Key Laboratory of Medicinal Chemical Biology Key Laboratory of Functional Polymer Materials of Ministry of Educationand Institute of Polymer ChemistryCollege of ChemistryNankai UniversityTianjin300071China

Bacterial biofilms present a significant challenge in treating drug-resistant infections,necessitating the development of innovative *** this study,we introduce triclosan-conjugated,lipase-responsive polymeric micelles designed to exploit biofilm properties and serve as a responsive drug delivery *** micelles were created using an amphiphilic block polymer synthesized via ring-opening polymerization ofε-caprolactone(CL)and triclosan-containing cyclic trimethylene carbonate(MTC-Tri).Poly(ethylene glycol)(PEG-OH)acted as the macro-initiator,resulting in micelles with a PEG shell that facilitated their penetration into bacterial *** important advantage of our micelles lies in their interaction with local bacterial lipases within *** lipases triggered rapid micelle degradation,releasing triclosan in a controlled *** liberated triclosan effectively eliminated bacteria embedded in the ***,the triclosan-conjugated micelles displayed minimal toxicity to murine fibroblasts,indicating their biocompatibility and *** finding emphasizes the potential application of these micelles in combatting drug resistance observed in bacterial *** triclosan-conjugated,lipase-responsive polymeric micelles exhibit promising characteristics for addressing drug resistance in bacterial *** harnessing biofilm properties and implementing a responsive drug delivery system,we seek to provide an effective solution in the fight against drug-resistant bacteria.

关键词： Self-assembly Polymer prodrug Ring-opening polymerization Biofilm eradication Cytotoxicity

Injectable hydrogel made from antler mesenchyme matrix for regenerative wound healing via creating a fetal-like niche

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World Journal of Stem Cells 2023年第7期15卷 768-780页

作者： Guo-Kun Zhang Jing Ren Ji-Ping li Dong-Xu Wang Sheng-Nan Wang li-yan shi Chun-Yi li Institute of Antler Science and Product Technology Changchun Sci-Tech UniversityChangchun 130600Jilin ProvinceChina College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun 130118Jilin ProvinceChina China-Japan Union Hospital Jilin UniversityChangchun 130033Jilin ProvinceChina

BACKGROUND Scar formation and loss of cutaneous appendages are the greatest challenges in cutaneous wound *** studies have indicated that antler reserve mesenchyme(RM)cells and their conditioned medium improved regenerative wound healing with partial recovery of cutaneous *** To develop hydrogels from the antler RM matrix(HARM)and evaluate the effect on wound *** We prepared the hydrogels from the HARM via enzymatic solubilization with *** we investigated the therapeutic effects of HARM on a full-thickness cutaneous wound healing rat model using both local injections surrounding the wound and topical wound *** The results showed that HARM accelerated wound healing rate and reduced scar ***,HARM stimulated the regeneration of cutaneous appendages and blood vessels,and reduced collagen fiber *** study showed that these functions might be achieved via creating a fetal-like niche at the wound *** levels of fetal wound healing-related genes,including Collagen III and TGFβ3 treated with HARM were all increased,while the expression levels of Collagen I,TGFβ1,and Engrailed 1 were decreased in the ***,the number of stem cells was increased in the fetal-like niche created by HARM,which may contribute to the regeneration of cutaneous *** Overall,we successfully developed an injectable hydrogel made from antler RM matrix for the regenerative repair of full-thickness cutaneous *** uncovered the molecular mechanism of the hydrogels in promoting regenerative wound healing,and thus pave the way for HARM to be developed for the clinic use.

关键词： Hydrogel Antler reserve mesenchyme Regenerative wound healing Fetal-like niche

Difference in microstructural characteristics of stamp-charged coke and gravity-charged coke with approximate macroscopic performance

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Journal of Iron and Steel Research International 2024年第5期31卷 1050-1062页

作者： Chong Zou Zheng-yan Huang Jian-yun li Bin li shi-wei liu Yuan She School of Metallurgical Engineering Xi’an University of Architecture and TechnologyXi’an710311ShaanxiChina Technical Center of Shandong Iron and Steel Co. Ltd.(Laiwu Branch)Jinan271104ShandongChina

The microstructural characteristics including optical texture,porosity and pore structure and chemical structure of stamp-charged coke(SCC)and gravity-charged coke(GCC)with similar conventional macro-indicators were investigated,and the properties including micro-strength,reactivity of coke matrix and that after alkali enrichment were comparatively studied by various characterization *** anisotropic structure of SCC is composed of high content of fine mosaic texture,while the content of medium mosaic texture,coarse texture and fibrous texture is *** statistical average shows that the fine mosaic average of SCC(24.89%)is 3.78 times the GCC average(6.58%),and the coarse mosaic average(1.24%)is only about 1/3 of the GCC average(3.43%).The porosity of SCC is lower than that of GCC,but tamping process does not lead to the fact that the number of closed pores of SCC is significantly lower than that of *** the structure of SCC is compact,its pore number is large and the pore wall is *** of coke with diameter less than 150 nm seem unaffected by tamping *** aromatic structure of SCC was less ordered than that of GCC,which was speculated to be related to the addition of more low metamorphic coal in *** microscopic strength and structural strength of SCC are lower than those of *** reactivity of coke matrix is affected by the specific surface area,but it is not the determining factor of its *** improvement in dissolution reactivity of coke after potassium enrichment is independent of coke type.

关键词： Stamp-charged coke Optical texture Porosity Microstructure Reactivity