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GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines

World Journal of Stem Cells 2024年第5期16卷 538-550页

作者： Yang-Zhou Jiang Lan-Yue Hu Mao-Shan Chen Xiao-Jie Wang Cheng-Ning Tan Pei-Pei Xue Teng Yu Xiao-Yan He li-xin xiang Yan-Ni Xiao Xiao-liang li Qian Ran Zhong-Jun li li Chen Laboratory of Radiation Biology Department of Blood TransfusionLaboratory Medicine CenterThe Second Affiliated HospitalThird Military Medical UniversityChongqing 400037China Hematopoietic Acute Radiation Syndrome Medical and Pharmaceutical Basic Research Innovation Center Ministry of Education of the People’s Republic of ChinaChongqing 400037China

BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(Fli1)have been identified as negative regulators of ***,the positive regulators of ANKRD26 are still *** To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 *** Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family *** to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(Fli1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activi

关键词： Ankyrin repeat domain containing 26 GATA binding protein 2 Thrombocytopenia 2 Transcriptional regulation Myeloid-derived cell lines

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新辅助治疗后临床完全缓解或近临床完全缓解直肠癌病人临床治疗策略

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中国实用外科杂志 2024年第7期44卷 751-756页

作者：李心翔翁俊勇复旦大学附属肿瘤医院大肠外科上海200032

对于直肠癌新辅助放化疗后达到临床完全缓解和(或)近临床完全缓解的病人,其临床治疗策略一直存在争议,包括全直肠系膜切除(TME)手术、局部切除和等待观察策略。TME手术带来了相关风险,而非手术治疗策略在实现了器官保留、改善病人生活... 详细信息

对于直肠癌新辅助放化疗后达到临床完全缓解和(或)近临床完全缓解的病人,其临床治疗策略一直存在争议,包括全直肠系膜切除(TME)手术、局部切除和等待观察策略。TME手术带来了相关风险,而非手术治疗策略在实现了器官保留、改善病人生活质量的同时,有望实现与TME相当的肿瘤学安全性。等待观察策略避免了不必要的手术创伤和严重并发症的风险,随着新辅助治疗完全缓解率的持续提高,等待观察策略在直肠癌综合治疗的地位也必将进一步提升。优化新辅助治疗策略、准确识别获益群体、提升完全缓解评估的精确性,以及优化随访监测和补救治疗机制,是器官保留肿瘤学安全性的重要保障。但须注意,如盲目选择等待观察,存在潜在局部复发和预后受损风险,故建议在具有丰富直肠癌综合治疗经验的临床中心开展,并加强医患沟通,提高随访依从性,保障治疗结局。

关键词：局部进展期直肠癌完全缓解等待观察器官保留新辅助治疗

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2024年V2版《美国国家综合癌症网络结肠癌临床实践指南》更新解读

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中国实用外科杂志 2024年第7期44卷 730-732,736页

作者：李心翔张瑞嘉复旦大学附属肿瘤医院大肠外科上海200032

2024年V2版《美国国家综合癌症网络结肠癌临床实践指南》的更新涵盖了诊断和治疗的多个领域,特别注重分子诊断技术的进步、免疫治疗的最新进展以及综合治疗方案的调整。在分子检测方面,指南推荐对疑似或已确诊的转移性腺癌病人进行更全... 详细信息

2024年V2版《美国国家综合癌症网络结肠癌临床实践指南》的更新涵盖了诊断和治疗的多个领域,特别注重分子诊断技术的进步、免疫治疗的最新进展以及综合治疗方案的调整。在分子检测方面,指南推荐对疑似或已确诊的转移性腺癌病人进行更全面的基因分析,包括罕见基因突变/融合如POLE/POLD1、RET和NTRK的检测,并继续关注HER2、RAS、BRAF等基因突变。对于循环肿瘤DNA(ctDNA)的应用,指南持保守态度,建议仅在临床试验中使用,并在Ⅱ、Ⅲ期及接受辅助治疗的病人中谨慎使用ctDNA监测。治疗更新方面,推荐携带POLE/POLD1突变及错配修复功能缺陷(dMMR)/微卫星高度不稳定(MSI-H)的病人接受免疫治疗,尤其适用于存在不可切除转移的情况。同时,取消了抗表皮生长因子受体治疗对左半结肠肿瘤的限制,拓宽至所有适合的KRAS/NRAS/BRAF野生型病人,并将曲妥珠单抗的使用从RAS和BRAF野生型病人中解除限制,扩展到所有HER2扩增的结肠癌病人。此外,针对dMMR/MSI-H型病人的术后辅助化疗也进行了重组和更新建议,明确了辅助化疗在提升生存率方面的潜在限制。

关键词：美国国家综合癌症网络结肠癌指南解读

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Influencing factors on elastic-plastic deformation of multi-layered surfaces under sliding contact

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中国有色金属学会会刊：英文版 2004年第z1期14卷 472-477页

作者： YAN li PAN xin-xiang XU Jiu-jun CHENG Dong Institute of Materials and Technology Dalian Maritime University China Institute of Materials and Technology Dalian Maritime University China Institute of Materials and Technology Dalian Maritime University China Institute of Materials and Technology Dalian Maritime University China

Stress distribution in the gradient multi-layered surface under a sliding contact was investigated using finite element method(FEM). The main structure parameters of layered surface discussed are total layer thickness,layer number and elastic modulus ratio of layer to the substrate. A model of multi-layered surface contact with rough slider was studied. The effect of the surface structure parameters on the elastic-plastic deformation was analyzed.

关键词： gradient multi-layered surface elastic-plastic deformation surface structure parameter FEM

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RIG-I： a multifunctional protein beyond a pattern recognition receptor

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Protein & Cell 2018年第3期9卷 246-253页

作者： Xiao-xiao Xu Han Wan li Nie Tong Shao li-xin xiang Jian-zhong Shao Key Laboratory for Cell and Gene Engineering of Zhejiang Province College of Life Sciences Zhejiang University Hangzhou 310058 China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology Qingdao 266235 China

It was widely known that retinoic acid inducible gene I （RIG-I） functions as a cytosolic pattern recognition receptor that initiates innate antiviral immunity by detecting exogenous viral RNAs. However, recent stud- ies showed that RIG-I participates in other various cellular activities by sensing endogenous RNAs under different circumstances. For example, RIG-I facilitates the therapy resistance and expansion of breast cancer cells and promotes T cell-independent B cell activation through interferon signaling activation by recognizing non-coding RNAs and endogenous retroviruses in certain situations. While in hepatocellular carcinoma and acute myeloid leukemia, RIG-I acts as a tumor suppressor through either augmenting STAT1 activation by competitively binding STAT1 against its negative regulator SliP1 or inhibiting AKT-mTOR signaling pathway by directly interacting with Src respectively. These new findings suggest that RIG-I plays more diverse roles in various cellular life activities, such as cell proliferation and differentiation, than previously known. Taken together, the function of RIG-I exceeds far beyond that of a pattern recognition receptor.

关键词： RIG-I viral RNA endogenous RNA immunity cancer

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重介分选密度智能控制系统在济二煤矿选煤厂的应用

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煤炭加工与综合利用 2024年第2期 1-6页

作者：朱绍文李新祥戴长官朱新奇徐鹏展兖矿能源济宁二号煤矿山东济宁272072 山东能源集团技术研究总院山东济南250000 华为技术有限公司广东深圳518116

阐述了济宁二号煤矿选煤厂针对重介分选密度控制问题,以数字化建设为基础,依托人工智能技术进行了智能化建设的探索。基于完善的生产传感器,采集原煤灰分、入洗煤量、磁性物含量、精煤灰分等生产数据入库保存,作为智能控制系统的数据基... 详细信息

阐述了济宁二号煤矿选煤厂针对重介分选密度控制问题,以数字化建设为基础,依托人工智能技术进行了智能化建设的探索。基于完善的生产传感器,采集原煤灰分、入洗煤量、磁性物含量、精煤灰分等生产数据入库保存,作为智能控制系统的数据基础。基于盘古大模型和优化求解技术构建重介分选密度设定值的智能推荐系统,并将推荐的分选密度自动下发到PLC系统执行,形成完整的生产闭环。生产实践表明,该系统控制得到的精煤产品灰分稳定性更高,精煤理论产率相比人工控制提升0.334%。

关键词：选煤厂重介质旋流器人工智能大模型分选密度控制

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Analysis of S-SEED’s characteristics in optical switch

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The Journal of China Universities of Posts and Telecommunications 2008年第4期15卷 91-94页

作者： CAO Yong-sheng YIN Xiao-li xin xiang-jun YU Chong-xiu Key Laboratory of Optical Communication and lightwave Technologies Beijing University of Posts and Telecommunications Ministry of Education Beijing 100876 China

This article introduces the basic structure of a symmetric self-electrooptic effect device （S-SEED）, and applies the Kirchoff＇ s current law and a purely equivalent capacitive model, to analyze S-SEED＇s switch characteristics. linear approximation and N-segment approximation are utilized to obtain S-SEED＇s voltage-time （V-T） and characteristics. Theoretical analysis is verified by simulations, and the results demonstrate that the precision of S-SEED＇s switch time can satisfy the requirement in applications with linear approximation. Moreover, the simulations compare S-SEED＇s switch characteristics with different input powers and input contrast ratios, which reveal that increasing input contrast ratio is an effective way to improve S-SEED＇s switch characteristics.

关键词： symmetry S-SEED optical switch V-T characteristics characteristic simulations

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Midkine accumulated in nucleolus of HepG2 cells involved in rRNA transcription

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World Journal of Gastroenterology 2008年第40期14卷 6249-6253页

作者： li-Cheng Dai li-Shan Min Zhi-Hong Ma Yong-Tao Xiao li-xin xiang Jian-Zhong Shao Huzhou keylaboratory of Molecular Medicine Huzhou Central HospitalHuzhou 3 13000 Zhejiang Province China College oflife Sciences Zhejiang University Hangzhou 310031 ZhejiangProvince China

AIM： To invesgate the ultrastructural location of midkine （MK） in nucleolus and function corresponding to its location. METHODS： To investigate the ultrastructural location of MK in nucleolus with immunoelectronic microscopy. To study the role that MK plays in ribosomal biogenesis by real-time PCR. The effect of MK on anti-apoptotic activity of HepG2 cells was studied with FITC- conjugated annexin V and propidium iodide PI double staining through FACS assay. RESULTS： MK mainly localized in the granular component （GC）, dense fibrillar component （DFC） and the border between the DFC and fibrillar center （FC）. The production of 45S precursor rRNA level was decreased significantly in the presence of MK antisense oligonucleotide in the HepG2 cells. Furthermore, it was found that exogenous MK could protect HepG2 from apoptosis significantly. CONCLUSION： MK was constitutively translocated to the nucleolus of HepG2 cells, where it accumulated and mostly distributed at DFC, GC components and at the region between FC and DFC, MK played an important role in rRNA transcription, ribosome biogenesis, and cell proliferation in HepG2 cells. MK might serve as a molecular target for therapeutic intervention of human carcinomas.

关键词： 45S rRNA Anti-apotosis Proliferation Midkine

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Transition metal clusters with precise numbers of atoms anchored on graphdiyne as multifunctional electrocatalysts for OER/ORR/HER:a computational study

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Rare Metals 2024年第7期43卷 3107-3117页

作者： xin-Yang liu Jing-Wei liu Gang li Jing-xiang Zhao College of Chemistry and Chemical Engineering Harbin Normal UniversityHarbin 150025China

Subnanometer metal clusters play an increasingly important role in heterogeneous catalysis due to their high catalytic activity and *** this work,by means of the density functional theory(DFT) calculations,the catalytic activities of transition metal clusters with precise numbers of atoms supported on graphdiyne(TM_(1-4)@GDY,TM=V,Cr,Mn,Fe,Co,Ni,Cu,Ru,Rh,Pd,Ir,Pt) were investigated for oxygen evolution reactions(OER),oxygen reduction reactions(ORR) and hydrogen evolution reactions(HER).The computed results reveal that the Pd_(2),Pd_(4) and Pt_(1) anchored graphdiyne can serve as trifunctional catalysts for OER/ORR/HER with the overpotentials of 0.49/0.37/0.06,0.45/0.33/0.12 and 0.37/0.43/0.01 V,respectively,while Pd_(1) and Pt_(2)@graphdiyne can exhibit excellent catalytic performance for water splitting(OER/HER) with the overpotentials of 0.55/0.17 and 0.43/0.03 *** addition,Ni_(1) and Pd_(3) anchored GDY can perform as bifunctional catalysts for metal-air cells(OER/ORR) and fuels cells(ORR/HER) with the overpotentials of 0.34/0.32 and 0.42/0.04 V,***,by precisely controlling the numbers of atoms in clusters,the TM_(1-4) anchored graphdiyne can serve as promising multifunctional electrocatalysts for OER/ORR/HER,which may provide an instructive strategy to design catalysts for the energy conversation and storage devices.

关键词： Density functional theory Graphdiyne Single-cluster catalysts Multifunctional electrocatalysts Overpotential

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CR6-interacting factor-1 contributes to osteoclastogenesis by inducing receptor activator of nuclear factor κB ligand after radiation

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World Journal of Stem Cells 2020年第3期12卷 222-240页

作者： li-xin xiang Qian Ran li Chen Yang xiang Feng-Jie li Xiao-Mei Zhang Yan-Ni Xiao ling-Yun Zou Jiang F Zhong Shengwen Calvin li Zhong-Jun li Laboratory Medicine Center Department of Blood TransfusionLab of Radiation BiologyThe Second Affiliated HospitalThird Military Medical UniversityChongqing400037China Department of Otolaryngology Keck School of MedicineUniversity of Southern CaliforniaLos AngelesCA 90033United States Bioinformatics Center College of Basic Medical SciencesThird Military Medical UniversityChongqing 400038China CHOC Children’s Research Institute Children’s Hospital of Orange CountyUniversity of CaliforniaIrvineCA 92868United States

BACKGROUND Radiation induces rapid bone loss and enhances bone resorption and adipogenesis, leading to an increased risk of bone fracture. There is still a lack of effective preventive or therapeutic method for irradiation-induced bone *** activator of nuclear factor κB ligand(RANKL) provides the crucial signal to induce osteoclast differentiation and plays an important role in bone resorption. However, the mechanisms of radiation-induced osteoporosis are not fully *** To investigate the role of CR6-interacting factor-1(Crif1) in osteoclastogenesis after radiation and its possible *** C57 BL/6 mice were exposed to Co-60 gamma rays and received 5 Gy of wholebody sublethal irradiation at a rate of 0.69 Gy/min. For in vitro study, mouse bone marrow mesenchymal stem/stromal cells(BM-MSCs) were irradiated with Co-60 at a single dose of 9 Gy. For osteoclast induction, monocyte-macrophage RAW264.7 cells were cocultured with mouse BM-MSCs for 7 d. Clus Pro and Inter Pro Surf were used to investigate the interaction interface in Crif1 and protein kinase cyclic adenosine monophosphate(c AMP)-activited catalytic subunit alpha complex. Virtual screening using 462608 compounds from the life Chemicals database around His120 of Crif1 was carried out using the program Autodock_vina. A tetrazolium salt(WST-8) assay was carried out to study the toxicity of compounds to different cells, including human BM-MSCs, mouse BMMSCs, and Vero *** Crif1 expression increased in bone marrow cells after radiation in *** of Crif1 in mouse BM-MSCs and radiation exposure could increase RANKL secretion and promote osteoclastogenesis in vitro. Deletion of Crif1 in BM-MSCs could reduce both adipogenesis and RANKL expression,resulting in the inhibition of osteoclastogenesis. Deletion of Crif1 in RAW264.7 cells did not affect the receptor activator of nuclear factor κB expression or osteoclast differentiation. Following treatment with protein

关键词： Irradiation Osteoporosis Bone marrow Mesenchymal stem cells Monocyte macrophage Bone

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