Midkine accumulated in nucleolus of HepG2 cells involved in rRNA transcription
Midkine accumulated in nucleolus of HepG2 cells involved in rRNA transcription作者机构：Huzhou keylaboratory of Molecular Medicine Huzhou Central HospitalHuzhou 3 13000 Zhejiang Province China College ofLife Sciences Zhejiang University Hangzhou 310031 ZhejiangProvince China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2008年第14卷第40期
页 面：6249-6253页
核心收录：
学科分类：1006[医学-中西医结合] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床]
基 金：The Medical Science Research Foundation of Zhejiang Province No.2004A083
主 题：45S rRNA Anti-apotosis Proliferation Midkine
摘 要：AIM： To invesgate the ultrastructural location of midkine （MK） in nucleolus and function corresponding to its location. METHODS： To investigate the ultrastructural location of MK in nucleolus with immunoelectronic microscopy. To study the role that MK plays in ribosomal biogenesis by real-time PCR. The effect of MK on anti-apoptotic activity of HepG2 cells was studied with FITC- conjugated annexin V and propidium iodide PI double staining through FACS assay. RESULTS： MK mainly localized in the granular component （GC）, dense fibrillar component （DFC） and the border between the DFC and fibrillar center （FC）. The production of 45S precursor rRNA level was decreased significantly in the presence of MK antisense oligonucleotide in the HepG2 cells. Furthermore, it was found that exogenous MK could protect HepG2 from apoptosis significantly. CONCLUSION： MK was constitutively translocated to the nucleolus of HepG2 cells, where it accumulated and mostly distributed at DFC, GC components and at the region between FC and DFC, MK played an important role in rRNA transcription, ribosome biogenesis, and cell proliferation in HepG2 cells. MK might serve as a molecular target for therapeutic intervention of human carcinomas.
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