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Expression, subcellular localization and protein-protein interaction of four isoforms of EcR/USP in the common cutworm

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Insect Science 2015年第1期22卷 95-105页

作者： li-xia huang Yan-Jun Gong Jun Gu Bao-Juan Zeng li-Hua huang Qi-li Feng Laboratory of Molecular and Developmental Entomology Guangdong Provincial Key Laboratory of Biotechnology for Plant DevelopmentSchool of Life Sciences South China Normal University Guangzhou China

Ecdysone receptor （EcR） and ultraspiracle （USP） form heterodimers to mediate ecdysteroid signaling during molting and metamorphosis. Various EcR/USP heterodimers have been reported. However, it is unclear what kind of EcR/USP combination is adopted by lepidopteran insects during the larval-pupal metamorphosis and whether the EcR/USP heterodimer varies among different tissues. To address these questions, two isoforms of each EcR and USP were cloned from the common cutworm, their messenger RNA expression patterns were examined by real-time quantitative polymerase chain reaction in different tissues during the larval-pupal metamorphosis and in the midgut in response to hormonal induction. Furthermore, their subcellular localization and protein-protein interaction were explored by transient expression and far-western blotting, respectively. All the four genes were significantly up-regulated in prepuae and/or pupae. The expression profiles of EcRB1 and USP1 were nearly identical to each other in the epidermis, fat body and midgut, and a similar situation also applied to EcRA and USP2. The three genes responded to 20-hydroxyecdysone （20E） induction except for USP2, and USP1 could be up-regulated by both 20E and juvenile hormone. The four proteins mainly localized in the nucleus and the nuclear localization was promoted by 20E. The protein-protein interaction between each EcR and USP was found in vitro. These results suggest that two types of EcR/USP heterodimer （EeRA/USP2 and EcRB 1/USP1） may exist simultaneously in the common cutworm, and the latter should play more important roles during the larval-pupal metamorphosis. In addition, the types of EcR/USP heterodimer do not vary in the tissues which undergo histolysis and regeneration during metamorphosis.

关键词： ecdysone receptor protein interaction subcellular localization ultraspiracle

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Multiple microRNAs control ecdysone signaling in the midgut of Spodoptera litura

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Insect Science 2020年第6期27卷 1208-1223页

作者： Wei Luo li-xia huang Shuang-Kang Qin xian Zhang Qi-li Feng Jun Gu li-Hua huang Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangzhou Key Laboratory of Insect Development Regulation and Application Research Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Hubei Key Laboratory of Application of Plant-Cancer Active Ingredients School of Chemistry and Life SciencesHubei University of EducationWuhanChina

Metamorphosis is one of the most important physiological processes in *** is regulated by a serial of ecdysone cascade ***,lots o f microRNAs(miRNAs)were investigated in insects;however,their function in metamorphosis is largely *** the present study,the dynamics of a small RNA population was investigated by RNA sequencing from the midgut of a lepidopteran pest Spodoptera litura during larval-pupal metamorphosis.A total of 101 miRNAs were identified,and 75 miRNAs were differentially expressed during the metamorphic *** relationship between these differentially expressed miRNAs and 12 ecdysone cascade genes was analyzed by four classical software programs,and a multiple-to-multiple regulatory network was found to exist between these miRNAs and their *** them,miR-14-3p and its two targets(EcR and E75)were chosen for further ***-14-3p had higher expression level in the 6 th instar larvae as compared with either the prepupae or pupae,which was opposite to that of both EcR and E75,two ecdysone cascade *** reporter assay confirmed that both EcR and E75 were regulated by ***,the 3'untranslated regions are nearly identical to each other among different transcript variants of the ecdysone cascade genes,including EcR,USP,E75,E74,E78,E93,Hr3,Hr4,Hr39,K rhl and F ***,different transcript variants of one ecdysone cascade gene could be regulated by the same mi *** above data suggest that the ecdysone signaling pathway is under the tight control of *** findings expand our understanding of the mechanism of insect metamorphosis and may also provide a novel possibility for the control of pest insects in the future.

关键词： ecdysone EcR E75 metamorphosis microRNA midgut

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Cloning, expression and chitin-binding activity of two peritrophin-like protein genes in the common cutworm, Spodoptera fitura

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Insect Science 2014年第4期21卷 449-458页

作者： Wei-Jun Chen li-xia huang Dan Hu li-Yan liu Jun Gu li-Hua huang Qi-li Feng Guangdong Provincial Key Laboratory of Biotechnology for Plant Development School of Life Sciences South China Normal UniversityGuangzhou China

Insect midgut secretes a semi-permeable peritrophic membrane （PM）, which plays important roles in protecting the midgut and helping with food digestion. The lep- idopteran larvae produce type 1 PM, which is degraded when insects develop into the metamorphic stages. However, the underlying mechanism is unclear. In the present study, two peritrophin-like proteins （peritrophin-57 and 37） were identified from the midgut expression sequence tag library and transcriptome of the common cutworm, Spodoptera litura. The temporal and spatial expression patterns and responses to the induction of 20- hydroxyecdysone （20E） and starvation were examined by real-time quantitative polymerase chain reaction according to their common sequence region. The chitin-binding activity was also studied using a competitor, calcofluor. The open reading frames are 1 554 and 1 020 bp, respectively. They shared four highly conserved peritrophin-A domains and were ex- pressed only in the midgut rather than in the other tissues, including fat body, epidermis, Malpighian tube and hemolymph. Their transcriptional expression could only be detected at the larval stages rather than in eggs, prepupae, pupae and adults. The purified protein of peritrophin-37 bound to chitin in a dose-dependent manner. These results indicate that the two proteins are peritrophins, the structural components of PM. In addition, the messenger RNA levels of the two peritrophins were significantly down-regulated by 20E injection, whereas feeding/starvation had no effect on the expression. These findings suggest that the increase of20E titer may be an important factor which controls the degradation of PM during metamorphosis.

关键词： metamorphosis peritrophic membrane peritrophin starvation 20-hydroxyecdysone

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微信平台护理管理对慢性乙型肝炎合并妊娠晚期患者负面情绪和生活质量的影响

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世界华人消化杂志 2018年第2期26卷 126-130页

作者：黄黎霞浙江省义乌市中心医院浙江省义乌市322000

目的观察微信平台护理管理对慢性乙型肝炎(chroni c hepatitis B,CHB)合并妊娠晚期患者负面情绪和生活质量的影响.方法选取2015-02/2017-02在浙江省义乌市中心医院产科住院的90例CHB合并妊娠晚期患者,采用随机数字表法分为对照组和观察... 详细信息

目的观察微信平台护理管理对慢性乙型肝炎(chroni c hepatitis B,CHB)合并妊娠晚期患者负面情绪和生活质量的影响.方法选取2015-02/2017-02在浙江省义乌市中心医院产科住院的90例CHB合并妊娠晚期患者,采用随机数字表法分为对照组和观察组各45例.对照组予以常规护理,观察组在对照组常规护理基础上采用微信平台护理.观察2组患者负面情绪和生活质量的变化情况.结果与干预前比较,2组焦虑自评量表、抑郁自评量表评分均降低(P<0.05),且微信平台干预后观察组评分分别为35.7分±4.8分和36.2分±4.9分,均明显低于对照组(P<0.05).与干预前比较,2组生活质量如躯体功能评分、心理功能评分、社会功能评分及物质功能评分均升高(P<0.05);且微信平台干预后观察组各量表评分均明显高于对照组(P<0.05).结论通过微信平台进行护理管理能降低CHB合并妊娠晚期患者负面情绪和提高患者生活质量,值得临床推广应用.

关键词：微信平台护理管理妊娠晚期慢性乙型肝炎负面情绪生活质量

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Protein nanoparticles as potent delivery vehicles for polycytosine RNA-binding protein one

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World Journal of Diabetes 2025年第1期16卷 222-225页

作者： Zi-Yu Zhao Pei-li Luo xia Guo Zheng-Wei huang State Key Laboratory of Bioactive Molecules and Druggability Assessment Guangdong Basic Research Center of Excellence for Natural Bioactive Molecules and Discovery of Innovative DrugsCollege of PharmacyJinan UniversityGuangzhou 511436Guangdong ProvinceChina

Ma et al recently reported in the World Journal of Diabetes that ferroptosis occurs in osteoblasts under high glucose conditions,reflecting diabetes *** condition could be protected by the upregulation of the gene encoding polycytosine RNA-binding protein 1(PCBP1).Additionally,Ma et al used a lentivirus infection system to express *** the authors’method of administration can be improved in terms of stability and cost,we propose delivering PCBP1 to treat type 2 diabetic osteoporosis by encapsulating it in protein ***,PCBP1 is small and ***,intravenous injection can help deliver PCBP1 across the mucosa while avoiding acid and enzyme-catalyzed ***,incorporating PCBP1 into nanoparticles prevents its interaction with water or oxygen and protects PCBP1’s structure and ***,the safety of the protein materials and the industrialization techniques for large-scale production of protein nanoparticles must be comprehensively investigated before clinical application.

关键词： Polycytosine RNA-binding protein 1 Protein nanoparticle Osteoblast Ferroptosis Diabetes

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第三方支付平台交易的粗糙博弈模型及算法研究

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运筹与管理 2016年第5期25卷 46-52,74页

作者：曹黎侠黄光球西安建筑科技大学管理学院陕西西安710055 西安工业大学理学院陕西西安710032

鉴于第三方支付平台交易的参与者在策略选择时存在着不可分辨现象和博弈,而目前粗糙博弈论的研究仍缺少系统化的理论成果。本文首先对照经典博弈论的概念,给出了粗糙博弈论的定义,然后创立了求解n人非合作完全信息静态粗糙博弈论的混合... 详细信息

鉴于第三方支付平台交易的参与者在策略选择时存在着不可分辨现象和博弈,而目前粗糙博弈论的研究仍缺少系统化的理论成果。本文首先对照经典博弈论的概念,给出了粗糙博弈论的定义,然后创立了求解n人非合作完全信息静态粗糙博弈论的混合策略均衡解的算法,最后建立了第三方支付平台交易的粗糙博弈模型,并给出模型的均衡解。应用实例表明模型和算法是有效可行的,比较符合当前第三方支付平台交易的实际状况和参与人的关系现状,模型的求解结果能够指导博弈各方更好地确定自己的策略。粗糙博弈论及非合作完全信息静态粗糙博弈论的混合策略均衡解的算法研究,给第三方支付平台交易的管理者提供了科学有益的借鉴。

关键词：粗糙博弈论混合策略均衡解算法粗糙集理论第三方支付平台交易

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Multi-Stage-Based Siamese Neural Network for Seal Image Recognition

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 405-423页

作者： Jianfeng Lu xiangye huang Caijin li Renlin Xin Shanqing Zhang Mahmoud Emam School of Computer Science and Technology Hangzhou Dianzi UniversityHangzhou310018China Shangyu Institute of Science and Engineering Hangzhou Dianzi UniversityShaoxing312300China Faculty of Artificial Intelligence Menoufia UniversityShebin El-Koom32511Egypt

Seal authentication is an important task for verifying the authenticity of stamped seals used in various domains to protect legal documents from tampering and *** seal inspection is commonly audited manually to ensure document ***,manual assessment of seal images is tedious and laborintensive due to human errors,inconsistent placement,and completeness of the *** image recognition systems are inadequate enough to identify seal types accurately,necessitating a neural network-based method for seal image ***,neural network-based classification algorithms,such as Residual Networks(ResNet)andVisualGeometryGroup with 16 layers(VGG16)yield suboptimal recognition rates on stamp ***,the fixed training data categories make handling new categories to be a challenging *** paper proposes amulti-stage seal recognition algorithmbased on Siamese network to overcome these ***,the seal image is pre-processed by applying an image rotation correction module based on Histogram of Oriented Gradients(HOG).Secondly,the similarity between input seal image pairs is measured by utilizing a similarity comparison module based on the Siamese ***,we compare the results with the pre-stored standard seal template images in the database to obtain the seal *** evaluate the performance of the proposed method,we further create a new seal image dataset that contains two subsets with 210,000 valid labeled pairs in *** proposed work has a practical significance in industries where automatic seal authentication is essential as in legal,financial,and governmental sectors,where automatic seal recognition can enhance document security and streamline validation ***,the experimental results show that the proposed multi-stage method for seal image recognition outperforms state-of-the-art methods on the two established datasets.

关键词： Seal recognition seal authentication document tampering siamese network spatial transformer network similarity comparison network

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The Relationship Among Photoinhibition, Photooxidation and Early Aging at a Late Development Stage in Different Varieties with High Yield

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Agricultural Sciences in China 2002年第6期1卷 618-625页

作者： JIAO De-mao, li xia, huang Xue-qing and JI Ben-hua( Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences , Nanjing 210014 , P.R. China Department of Biology, Nantong Normal College, Nantong 226007 , P.R. China) Institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences Nanjing 210014 P. R. China

Using six high yield varieties from different ecological districts in China the parameters of Chl fluorescence, the performance of membrane lipid peroxidation and the reduction of Chl content in flag leaf under natural condition at the later developmental stages (from heading stage to mature stage). The results showed that Fv/Fm , decreased gradually, the excessive light energy led to the accumulation of active oxygen O2-, H2O2 and the product of membrane-lipid peroxidation, MDA, which resulted in the reduction of Chl content and early aging due to photooxidation during the course of senescence of flag leaf. This phenomenon varied obviously in rice varieties. When comparina japonica tolerant to photooxidation, Fv/Fm in indica shanyou 63 susceptible to photoxidation decreased significantly. An increase of active oxygen and a sharp drop of Chl content, resulted in 'yellowish' early aging and influenced filling and setting of rice grain. The mechanism on early aging in indica was related to light and temperature conditions in filling stages. On a fine day (above 251), PS Ⅱ reaction center exhibited a dynamic change on revisable inactivation. Under strong midday light, PS Ⅱ function in indica exhibited obvious down-regulation and photoinhibition; Under strong light with low temperature, PS Ⅱ resulted in photodamage, showing early aging, which were related to the degradation of PSⅡ - D1 Protein and the inhibition of the endogenous protecting system such as Xanthophyll cycle and the enzymes of scavenging active oxygen. The results suggested that for a view of high-yield breeding, on the basis of a good type-plant, giving consideration to the utilization of heterosis and resistance to early aging, selecting japonica or a sterile line with japonice genotype as maternal was a breeding strategy worthy of being paid more attention.

关键词： Rice Characteristics of chl fluorescence Photoinhibition Photooxidation Early aging

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安徽省部分城市土壤中全氟化合物空间分布及来源解析

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环境科学 2017年第1期38卷 327-332页

作者：李法松倪卉黄涵宇徐志兵张倩李长霞黄文秀金陶胜安庆师范大学资源环境学院安庆246011 南开大学环境科学与工程学院教育部环境污染过程与基准教育部重点实验室天津300071 中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室北京100085

为探究安徽省城市土壤中全氟化合物(perfluorinated compounds,PFCs)的污染特征、空间分布及潜在的来源.2013年采集安徽省7个城市共11个土壤样品,利用超高效液相色谱-串联质谱联用分析了15种PFCs的含量,并运用主成分分析-线性回归法解... 详细信息

为探究安徽省城市土壤中全氟化合物(perfluorinated compounds,PFCs)的污染特征、空间分布及潜在的来源.2013年采集安徽省7个城市共11个土壤样品,利用超高效液相色谱-串联质谱联用分析了15种PFCs的含量,并运用主成分分析-线性回归法解析其可能来源.结果表明,被调查的11个城市土壤样品中的ΣPFCs的含量(以干重计,下同)范围为1.15~5.89 ng·g-1,平均含量2.69 ng·g-1.含量最高的单体PFCs是全氟辛烷磺酸(perfluorooctane sulfonate,PFOS),含量范围为n.d.~3.56 ng·g-1,均值含量为0.96 ng·g-1,其次是全氟辛烷羧酸(perfluorooctane acid,PFOA),含量范围为n.d.~2.89ng·g-1,均值含量为0.64 ng·g-1;ΣPFCs含量最高地区是滁州地区(5.89 ng·g-1),以PFOS和PFOA为主,其次为宣城市泾县(4.04 ng·g-1),而其中PFOS含量高达3.56 ng·g-1,占ΣPFCs的88.1%,这可能与该地发达的造纸行业有关;与我国其他地区土壤中PFCs含量相比,安徽省被调查地区的城市土壤中PFCs的含量居于中等水平;安徽省部分城市土壤中60%的PFCs可以有4个因子进行解释,代表性PFCs有PFOA、全氟丁烷磺酸(perfluorobutane sulfonate,PFBS)、全氟十二烷羧酸(perfluorododecanoic acid,PFDo A)、全氟丁烷羧酸(perfluorobutane acid,PFBA)和PFOS.

关键词：全氟化合物空间分布来源安徽省城市土壤

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The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice

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Chinese Science Bulletin 2001年第13期46卷 1080-1084页

作者： JIAO Demao, li xia, huang Xueqing, CHI Wei, KUANG Tingyun & Ku SB Maurice1. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nangjing 210014, China 2. Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China 3. Botany Department, Washington State University, Pullman, WA 99164-4236, USA Institute of Agrobiological Genetics and Physiology Jiangsu Academy of Agricultural Sciences Nangjing China Institute of Botany The Chinese Academy of Sciences Beijing China Botany Department Washington State University Pullman USA

With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3 h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC

关键词： transgenic rice photosynthetic characteristics photoin-hibition of photosynthesis chlorophyll fluorescence phosphoenopy-ruvate carboxylase (PEPC).

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