Multiple microRNAs control ecdysone signaling in the midgut of Spodoptera litura
在 Spodoptera litura 的 midgut 发信号的多重 microRNAs 控制蜕皮激素作者机构：Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologyInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Hubei Key Laboratory of Application of Plant-Cancer Active IngredientsSchool of Chemistry and Life SciencesHubei University of EducationWuhanChina
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2020年第27卷第6期
页 面：1208-1223页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：grants from the National Natural Science Foundation of China(Grant No.31672357 and 31872285)
主 题：ecdysone EcR E75 metamorphosis microRNA midgut
摘 要：Metamorphosis is one of the most important physiological processes in *** is regulated by a serial of ecdysone cascade ***,lots o f microRNAs(miRNAs)were investigated in insects;however,their function in metamorphosis is largely *** the present study,the dynamics of a small RNA population was investigated by RNA sequencing from the midgut of a lepidopteran pest Spodoptera litura during larval-pupal metamorphosis.A total of 101 miRNAs were identified,and 75 miRNAs were differentially expressed during the metamorphic *** relationship between these differentially expressed miRNAs and 12 ecdysone cascade genes was analyzed by four classical software programs,and a multiple-to-multiple regulatory network was found to exist between these miRNAs and their *** them,miR-14-3p and its two targets(EcR and E75)were chosen for further ***-14-3p had higher expression level in the 6 th instar larvae as compared with either the prepupae or pupae,which was opposite to that of both EcR and E75,two ecdysone cascade *** reporter assay confirmed that both EcR and E75 were regulated by ***,the 3 untranslated regions are nearly identical to each other among different transcript variants of the ecdysone cascade genes,including EcR,USP,E75,E74,E78,E93,Hr3,Hr4,Hr39,K rhl and F ***,different transcript variants of one ecdysone cascade gene could be regulated by the same mi *** above data suggest that the ecdysone signaling pathway is under the tight control of *** findings expand our understanding of the mechanism of insect metamorphosis and may also provide a novel possibility for the control of pest insects in the future.
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