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Saline conducted electric coagulation (SCEC):original experience in experimental hepatectomy

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第3期13卷 186-191页

作者： Guo-ping DING li-ping cao Da-ren liU Ri-sheng QUE Department of General Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective: To evaluate the feasibility and superiority of a new coagulating and hemostatic method named "saline conducted electric coagulation (SCEC)". Methods: The Peng's multifunction operative dissector (PMOD) was modified to enable saline to effuse persistently out of its nib at a constant speed. In a group of six New Zealand rabbits, two hepatic lobes of each rabbits were resected respectively by SCEC and conventional electric coagulation (EC). The features of SCEC were recorded by photo and compared with conventional EC. After 7 d, the coagulating depth was measured in each residual hepatic lobe. Hepatic tissue was dyed by hematoxylin and eosin (HE) and studied under a microscope. Results: The coagulating depth increased with the continuation of SCEC time. Hepatectomies were performed successfully, no rabbit died in the perioperative period. The incisal surface of SCEC was gray-white with no red bleeding point. There was a thick solidified layer at the margin and a thin red-white intermittent layer between the solidified layer and normal hepatic tissue at the vertical section of SCEC. The mean coagulating depth of SCEC was 1.8 cm vs. 0.3 cm of conventional EC. Pathological examination showed a mild inflammatory reaction by SCEC. Conclusions: SCEC is a feasible and safe method for surgical hemostasis. As a new technique for liver resection, SCEC shows better coagulating effect and milder inflammatory reaction than conventional EC. Our study shows bloodless liver resection can also be performed by SCEC, especially for liver malignant tumor.

关键词： Saline Electric coagulation Hepatectomy Hemostasis Bloodless liver resection

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Estimation of Physiologic Ability and Surgical Stress scoring system for predicting complications following abdominal surgery: A metaanalysis spanning 2004 to 2022

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World Journal of Gastrointestinal Surgery 2024年第1期16卷 215-227页

作者： Tian-Shu Pang li-ping cao Department of General Surgery Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016Zhejiang ProvinceChina

BACKGROUND Postoperative complications remain a paramount concern for surgeons and healthcare *** To present a comprehensive analysis of the Estimation of Physiologic Ability and Surgical Stress(E-PASS)scoring system’s efficacy in predicting postoperative complications following abdominal *** A systematic search of published studies was conducted,yielding 17 studies with pertinent *** such as preoperative risk score(PRS),surgical stress score(SSS),comprehensive risk score(CRS),postoperative complications,post-operative mortality,and other clinical data were collected for *** plots were employed for continuous and binary variables,withχ2 tests assessing heterogeneity(P value).RESULTS Patients experiencing complications after abdominal surgery exhibited significantly higher E-PASS scores compared to those without complications[mean difference and 95%confidence interval(CI)of PRS:0.10(0.05-0.15);SSS:0.04(0.001-0.08);CRS:0.19(0.07-0.31)].Following the exclusion of low-quality studies,results remained valid with no discernible *** analysis indicated that variations in sample size and age may contribute to hetero-geneity in CRS *** variable meta-analysis demonstrated a correlation between high CRS and increased postoperative complication rates[odds ratio(OR)(95%CI):3.01(1.83-4.95)],with a significant association observed between high CRS and postoperative mortality[OR(95%CI):15.49(3.75-64.01)].CONCLUSION In summary,postoperative complications in abdominal surgery,as assessed by the E-PASS scoring system,are consistently linked to elevated PRS,SSS,and CRS *** CRS scores emerge as risk factors for heightened morbidity and *** study establishes the accuracy of the E-PASS scoring system in predicting postoperative morbidity and mortality in abdominal surgery,underscoring its potential for widespread adoption in effective risk assessment.

关键词： Estimation of Physiologic Ability and Surgical Stress scoring system Preoperative risk score Surgical stress score Comprehensive risk score Complications

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Transumbilical single-port laparoscopic cholecystectomy using traditional laparoscopic instruments:a report of thirty-six cases

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第10期12卷 862-866页

作者： li-ping cao Ri-sheng QUE Fan ZHOU Guo-ping DING De-xi JING Department of Surgery the Second Affliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China

Objective:To evaluate the feasibility and safety of the operation of transumbilical single-port laparoscopic cholecystectomy (TSPLC) by traditional laparoscopic instruments and summarize the initial ***:Sixty subjects with cholelithiasis were divided into two *** group (36 cases) underwent TSPLC and the control group (24 cases) underwent traditional three-port laparoscopic cholecystectomy (LC).Postoperative complications were observed and operation time,hospital days,visual analogue scale (VAS) after 6 and 24 h of operation,and subject satisfaction score were ***:TSPLC and traditional LC were performed successfully in the two *** operation time in the TSPLC group was significantly longer than that in the control *** was no statistically significant difference in hospital stay and VAS between the TSPLC and control *** subject satisfaction score in the TSPLC group was 91.2,significantly higher than that in the control group (Plication occurred during the period of two weeks after ***:TSPLC is a feasible and safe method for cholecystectomy,although it may be more ***,it is welcomed by patients who are more concerned with cosmetic *** studies are needed to confirm its disadvantages and contraindications.

关键词： Laparoscopic cholecystectomy Single-incision laparoscopic cholecystectomy Minimally invasive surgery Traditional laparoscopic instruments

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Effect of lead on ERK activity and the protective function of bFGF in rat primary culture astroglia

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第6期8卷 422-427页

作者： ZHANG Ying YE li-ping WANG Biao cao Shi-cheng SUN li-guang Department of Biochemistry and Molecule Biology College of Basic Medical Sciences China Medical University. Shenyang 110001 China

Objective： To observe the effects of lead on levels ofphosphorylated extracellular signal regulated kinase （p-ERK） in the cytoplasm of primary cultures of rat astroglial cells and the possible protective effect of basic fibroblast growth factor （bFGF） on lead-induced effects. Methods： The primary astroglia cells from 1-6 d old Wistar rats were cultured. The cells pretreated with the MEK1 （mitogen-activated protein kinase kinase 1） inhibitor PD98059 and bFGF, respectively, were exposed to Pb acetate of different concentrations for different times. Western blotting and reverse transcription polymerase chain reaction （RT-PCR） methods were used to detect the protein and mRNA expressions of ERK. Results： mRNA expression for ERK peaked 15 min after initiation of lead exposure （P〈0.05） and protein expression of p-ERK peaked at 30 min （P〈0.05）. ERK mRNA levels and p-ERK protein levels returned to baseline after 60 and 120 min of lead exposure, respectively （P〉0.05）. The increase in p-ERK levels in lead-treated cells could be inhibited by PD098059. Activation of ERK in the cells by lead was prevented by pretreatment with bFGF. Total ERK protein levels did not change under the same experimental conditions （P〉0.05）. Conclusion： Low-level lead exposure resulted in transient activation of ERK through the MEK pathway, which then returned to basal levels in the continued presence of lead. Exogenous bFGF protected ERK signaling components in astroglia from lead poisoning.

关键词： Lead Astroglia Extracellular signal regulated kinase (ERK) Basic fibroblast growth factor (bFGF)

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Cholangiocarcinoma-derived exosomes inhibit the antitumor activity of cytokine-induced killer cells by down-regulating the secretion of tumor necrosis factor-α and perforin

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第7期17卷 537-544页

作者： Jiong-huang CHEN Jian-yang XIANG Guo-ping DING li-ping cao Department of General Surgery Sir Run Run Shaw Hospital School of Medicine Zhejiang University

Objective： The aim of our study is to observe the impact of cholangiocarcinoma-derived exosomes on the antitumor activities of cytokine-induced killer （CIK） cells and then demonstrate the appropriate mechanism. Methods： Tumor-derived exosomes （TEXs）, which are derived from RBE cells （human cholangiocarcinoma line）, were collected by ultracentrifugation. CIK cells induced from peripheral blood were stimulated by TEXs. Fluorescence-activated cell sorting （FACS） was performed to determine the phenotypes of TEX-CIK and N-CIK （normal CIK） cells. The concen- trations of tumor necrosis factor-a （TNF-a） and perforin in the culture medium supematant were examined by using an enzyme-linked immunosorbent assay （EliSA） kit. A CCK-8 kit was used to evaluate the cytotoxic activity of the CIK cells to the RBE cell line. Results： The concentrations of TNF-a and perforin of the group TEX-CIK were 138.61 pg/ml and 2.41 ng/ml, respectively, lower than those of the group N-CIK 194.08 pg/ml （P〈0.01） and 3.39 ng/ml （P〈0.05）. The killing rate of the group TEX-CIK was 33.35%, lower than that of the group N-CIK （47,35% （P〈0.01））. The population of CD3＋, CD8＋, NK （CD56＋）, and CD3＋CD56＋ cells decreased in the TEX-CIK group （63.2±6.8）%, （2.5±1.0）%, （0.53±0.49）%, （0.45±0.42）%） compared with the N-CIK group （（90.3±7.3）%, （65.7±3.3）%, （4.2±1.2）%, （15.2±2.7）%）, P〈0.01. Conclusions： Our results suggest that RBE cells-derived exosomes inhibit the antitumor activity of CIK cells by down-regulating the population of CD3＋, CD8＋, NK （CD56＋）, and CD3＋CD56＋ cells and the secretion of TNF-a and perforin. TEX may play an important role in cholangiocarcinoma immune escape.

关键词： Cholangiocarcinoma Tumor-derived exosomes Cytokine-induced killer cells Immune escape

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Increasing the immune activity of exosomes: the effect of miRNA-depleted exosome proteins on activating dendritic cell/cytokine-induced killer cells against pancreatic cancer

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第5期17卷 352-360页

作者： Ri-sheng QUE Cheng liN Guo-ping DING Zheng-rong WU li-ping cao Department of Surgery the Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310009 China Department of Surgery Sir Run Run Shaw Hospital School of Medicine Zhejiang University Hangzhou 310020 China

Background： Tumor-derived exosomes were considered to be potential candidates for tumor vaccines because they are abundant in immune-regulating proteins, whereas tumor exosomal miRNAs may induce immune tolerance, thereby having an opposite immune function. Objective： This study was designed to separate exosomal protein and depleted exosomal microRNAs （miRNAs）, increasing the immune activity of exosomes for activating dendritic cell/cytokine-induced killer cells （DC/CIKs） against pancreatic cancer （PC）. Methods： PC-derived exosomes （PEs） were extracted from cultured PANC-1 cell supernatants and then ruptured; this was followed by ultrafiltered exosome lysates （UELs）. DCs were stimulated with lipopolysaccharide （LPS）, PE, and UEL, followed by co-culture with CIKs. The anti-tumor effects of DC/CIKs against PC were evaluated by proliferation and killing rates, tumor ne- crosis factor-a （TNF-a） and perforin secretion. Exosomal miRNAs were depleted after lysis and ultrafiltration, while 128 proteins were retained, including several immune-activating proteins. Results： UEL-stimulated DC/CIKs showed a higher killing rate than LPS- and PE-stimulated DC/CIKs. Conclusions： miRNA-depleted exosome proteins may be promising agonists for specifically activating DC/CIKs against PC.

关键词： Pancreatic cancer Exosome Dendritic cell MicroRNAs

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Gut microbiota-astrocyte axis: new insights into age-related cognitive decline

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Neural Regeneration Research 2025年第4期20卷 990-1008页

作者： Lan Zhang Jingge Wei Xilei liu Dai li Xiaoqi Pang Fanglian Chen Hailong cao ping Lei Haihe Laboratory of Cell Ecosystem Department of GeriatricsTianjin Medical University General HospitalTianjinChina Tianjin Geriatrics Institute Tianjin Medical University General HospitalTianjinChina Department of Gastroenterology and Hepatology Tianjin Medical University General HospitalNational Key Clinical SpecialtyTianjin Institute of Digestive DiseasesTianjin Key Laboratory of Digestive DiseasesTianjinChina Department of Urology Tianjin Medical University General HospitalTianjinChina Tianjin Neurological Institution Tianjin Medical University General HospitalTianjinChina

With the rapidly aging human population,age-related cognitive decline and dementia are becoming increasingly prevalent *** is considered the main risk factor for cognitive decline and acts through alterations in the composition of the gut microbiota,microbial metabolites,and the functions of *** microbiota–gut–brain axis has been the focus of multiple studies and is closely associated with cognitive *** article provides a comprehensive review of the specific changes that occur in the composition of the gut microbiota and microbial metabolites in older individuals and discusses how the aging of astrocytes and reactive astrocytosis are closely related to age-related cognitive decline and neurodegenerative *** article also summarizes the gut microbiota components that affect astrocyte function,mainly through the vagus nerve,immune responses,circadian rhythms,and microbial ***,this article summarizes the mechanism by which the gut microbiota–astrocyte axis plays a role in Alzheimer’s and Parkinson’s *** findings have revealed the critical role of the microbiota–astrocyte axis in age-related cognitive decline,aiding in a deeper understanding of potential gut microbiome-based adjuvant therapy strategies for this condition.

关键词： age aging Alzheimer’s disease astrocytes cognitive decline dementia gut microbiota gut–brain axis microbial metabolites neuroinflammation Parkinson’s disease

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Combined gastroscopic and choledochoscopic transabdominal nasobiliary drainage

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第11期20卷 940-944页

作者： Song-mei LOU Min ZHANG Zheng-rong WU Gui-xing JIANG Hua SHEN Yi DAI Yue-long liANG li-ping cao Guo-ping DING Department of Hepatobiliary&Pancreatic Surgery Sir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou 310016China Department of General Surgery Huzhou Central HospitalHuzhou 313000China

Common bile duct(CBD)stones are a frequent problem in Chinese populations,and their incidence is particularly high in certain areas(Wang et al.,2013).In recent years,laparoscopic common bile duct exploration(LCBDE)and endoscopic retrograde cholangiopancreatography(ERCP)have been the main surgical procedures for CBD stones,although each has different advantages and disadvantages in the treatment of choledocholithiasis(Loor et al.,2017;Zhou et al.,2017).

关键词：胆道镜胃镜腹腔镜胆总管探查术(LCBDE) 鼻胆管引流

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Chaotic Method for Radio Fuze Signal Detection in Strong Noise

Chaotic Method for Radio Fuze Signal Detection in Strong Noi...

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2010 International Conference on Computer,Mechatronics, Control and Electronic Engineering (CMCE 2010)

作者： Yan Xiaopeng li ping cao Zhiyu Xu Fengkai Meng Feifei Laboratory for Mechatronic Engineering and Control, Beijing Institute of Technology, Beijing, China

The radio fuze signal is always buried in the noise, so it is difficult to detect useful information. A new method based on the chaotic theory is introduced to detect the radio fuze signal buried in the strong noise. Starting from the analysis of the chaotic detection theory, we established the chaotic system of the radio fuze signal, analyzed the influence for chaotic system from the radio fuze signal, and put forward the chaotic detection method. Then, an exposition was made on why the chaotic system can be used to improve signal-to-noise ratio. Simulink results show the new method can detect radio fuze signal in the strong noise efficiently.

关键词： duffing oscillator radio fuze denoising signal-to-noise ratio

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塔里木盆地巴楚凸起构造特征及含油气远景

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新疆石油地质 2001年第5期22卷 411-413页

作者：左龙凭李铁曹杨中国石化新疆石油公司西北石油局规划设计研究院新疆乌鲁木齐830011

塔里木盆地巴楚凸起地震地质条件较复杂，勘探程度较低。通过对巴楚凸起全区地震地质资料解释研究，发现了一些新的构造圈闭，认为该区的断裂及局部构造成排成带分布。在划分了构造带，描述了构造的形态和圈闭类型的基础上，对油气的控... 详细信息

塔里木盆地巴楚凸起地震地质条件较复杂，勘探程度较低。通过对巴楚凸起全区地震地质资料解释研究，发现了一些新的构造圈闭，认为该区的断裂及局部构造成排成带分布。在划分了构造带，描述了构造的形态和圈闭类型的基础上，对油气的控制作用及各构造带含油气远景有了一些新的认识，为该区今后的勘探描述提供了依据。

关键词：塔里木盆地巴楚凸起构造带构造圈闭断裂油气资源评价地震地质资料

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