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Chemical Research in Chinese Universities 2013年第2期29卷 206-209页

作者： WU Yu-sheng MA Jiao li ming-chun HU fang School of Materials Science and Engineering Shenyang University of Technology Shenyang 110870 P. R. China

γ-Al2O3 with high surface area and large pore volume combined with high thermal stability was synthe- sized by a reverse precipitation-azeotropic distillation method. The effects of azeotropic distillation on the characte- ristics of γ-Al2O3 were studied by means of X-ray diffraction（XRD）, Fourier transform infrared（FTIR） spectroscopy, transmission electron microscopy（TEM） and N2 adsorption-desorption. The results show that γ-Al2O3 dried by azeo- tropic distillation has excellent structure characteristics with a high surface area of 426 m2/g and a large pore volume of 2.56 cm3/g. After calcination at 1100 ℃, the surface area of γ-Al2O3 was still 92 mE/g with a large pore volume of 1.00 cma/g, indicating the potential application in catalyst and petroleum industry.

关键词： Reverse precipitation Azeotropic distillation y-Alumina n-Butyl alcohol Thermal stability

Molecular mechanism of temperature-dependent sex determination and differentiation in Chinese alligator revealed by developmental transcriptome profiling

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Science Bulletin 2018年第4期63卷 209-212页

作者： Jian-Qing lin Qi Zhou Hai-Qiong Yang li-ming fang Ke-Yi Tang li Sun Qiu-Hong Wan Sheng-Guo fang The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education and State Conservation Center for Gene Resources of Endangered WildlifeCollege of Life SciencesZhejiang UniversityHa ngzhou 310058China life Sciences Institute Zhejiang UniversityHangzhou 310058China Changxing Yinjiahian Chinese Alligator Nature Reserve Changxing 313100China

Sex determination(SD)is a critical embryonic development process that has profound impacts on the morphology of individuals and the survival of populations[1].In vertebrates,sex can be determined by either genetic or environmental factors[2],with the underlying molecular mechanism unknown for most species except for a few model organisms[3,4].Unlike mammals and birds,

关键词：温度依赖短吻鳄性别中国机制分子性染色体脊椎动物

Molecular characterization of two novel esterase genes from carmine spider mite, Tetranychus cinnabarinus （Acarina： Tetranychidae）

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Insect Science 2010年第2期17卷 91-100页

作者： Wei Sun Chuan-Hua Xue lin He Wen-Cai Lu ming li Xiao-fang Cao Zhi-Mo Zhao Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing China

Two novel esterase complementary DNAs were identified and cloned from the insecticide-susceptible strain of Tetranychus cinnabarinus （Boisduval）（Acarina： Tetrany- chidae）, which were designated as TCE1 and TCE2, respectively. The cDNA of TCE1 gene contained an open reading frame （ORF） of 1701 bp encoding 567 amino acids, and a predicted molecular weight of 62.75 kDa, the cDNA of TCE2 contained an ORF of 1680 bp encoding 560 amino acids, and a predicted molecular weight of 63.14 kDa. TCE1 and TCE2 were submitted to GenBank, accession number EU 130461 and EU 130462. The well-conserved sequence motif, GXSXG, used as a signature pattern in the esterase fam- ily are present in both TCE1 and TCE2 （GQSAG in TCE1, whereas GESAG in TCE2）, indicating that these two genes are predicted to be esterases. Comparison of the deduced amino acid sequence with the published mite esterase sequence coming from Boophilus microplus showed that TCE1 shares 33.98% identity and TCE2 shares 33.46% identity. TCE1 and TCE2 share 46.4% identity. Quantitative real-time polymerase chain reaction revealed that expression level of the TCE2 gene was relatively higher than that of the TCE1 in all instars examined except the protonymph, and the expression level of these two esterase genes in adults of T. cinnabarinus was significantly higher than that in any other instars, respectively. T. cinnabarinus is an important agricultural mite pest and esterases are important in the metabolisms of insects and mites; the genomic information obtained in this study will contribute to esterase molecular biological study on mite pest species.

关键词： esterase gene molecular cloning quantitative real-time PCR Tetranychus cinnabarinus

Effects of Soybean Isoflavones on In vitro Antioxidative Capacity of Satellite Cells of Porcine Skeletal Muscles

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Agricultural Sciences in China 2011年第1期10卷 120-125页

作者： JIANG Zong-yong ZHOU Gui-lian liN Ying-cai JIANG Shou-qun ZHENG Chun-tian HAN li-ming CHEN fang Key Laboratory of Animal Nutrition and Feed Science （South China） Ministry of Agriculture/Institute of Animal Science Guangdong Academy of Agricultural Science Guangzhou 510640 P.R.China

A synthetic isoflavone （ISO-S） or genistein was added in culture medium at different concentrations （0, 10, 20, 30, 40, and 80 *** L^-1） to investigate the effects of soybean isoflavones on antioxidative capacity of porcine skeletal muscle satellite cells. After 48 h incubation, the suspension was cryopreserved for the determination of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GSH-Px） activities, and malondialdehyde （MDA） content. The mRNA levels of SOD, CAT, and GSH-Px gene in cells were detected with Taqman fluorescent probe method. The results showed that the content of MDA and the activities and the mRNA levels of SOD of porcine skeletal muscle satellite cells were influenced by supplemented soybean isoflavone （P〈0.05） when adding 10-80 μmol L^-1 ISO-S or genistein in the medium. The MDA contents, SOD and CAT activities and their mRNA expression levels of porcine skeletal muscle cells responded quadratically （P〈 0.05） as the level of ISO-S or genistein increased. Pre-incubation of porcine skeletal muscle satellite cells with ISO-S or genistein at 10-40 pmol L-1 elevated the activities and the mRNA expression levels of SOD and CAT in cells concurrently and decreased the cellular content of MDA （P〈 0.05）. The results indicated that pre-incubation of ISO-S or genistein at 10- 40μmol L^-1 could improve the antioxidative capacity of porcine skeletal muscle satellite cells.

关键词： soybean isoflavone antioxidative capacity superoxide dismutase catalase skeletal muscle satellite cells

Biochemical pathways in the antiatherosclerotic effect of berberine

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Chinese Medical Journal 2008年第13期121卷 1197-1203页

作者： GUO Yi WANG Qi-zhang li fang-ming JIANG Xin ZUO Yan-fang WANG ling Department of Neurology Second Affiliated Hospital of Jinan University ShenzhenGuangdong 518020 China Department of Neurology Affiliated Shenzhen Shajing Hospital of Guangzhou Medical College Shenzhen Guangdong 518104 China Department of Neurology Union Hospital Tongji Medical College Huazhong University of Science and Technology Wtthan Hubei 430030 China Department of Cardiology Second Affiliated Hospital of Jinan University ShenzhenGuangdong 518020 China

Background This study investigated the inhibitory effect of berberine （BBR） on lipopolysaccharide （LPS） induced cyclooxygenase-2 （COX-2） expression via the mitogen activated protein kinase （MAPK） signalling cascade pathways in human peripheral blood monocytes （PBMC）. Methods PBMC from whole blood were isolated and cultured for up to 24 hours after division into 5 groups treated with LPS, LPS＋BBR 25 μmol/L, LPS＋BBR 50 μmol/L or LPS＋BBR 100 μmol/L and untreated. Monocytes were extracted for RT-PCR and Western blot analyses to examine COX-2 mRNA and protein activated expression of p38 mitogen activated protein kinase （p38MAPK）, Jun N-terminal kinase （JNK） and extracellular regulated kinases 1/2 （ERK1/2） signalling pathways. Results COX-2 mRNA and protein expression decreased to a minimum at 12 hours after BBR treatment （P 〈0.05）. With the increasing concentration of BBR treatment, the COX-2 expression decreased progressively （P 〈0.01）. With BBR treatment for 6, 12 or 24 hours at three doses, ERK1/2 protein expression was significantly inhibited. For the JNK pathway, only with the treatment of BBR at the concentration of 100 μmol/L was JNK protein expression inhibited compared with the LPS stimulation group （P 〈0.01）. Irrespective of the BBR concentration, no difference was shown between the BBR group and the LPS group for p38MAPK protein expression. Human monocytes COX-2 mRNA, by RT-PCR, and protein expression, by Western blot analysis, were inhibited when incubated with PD98059, SP600125 and SB203580 （P 〈0.05）. Conclusions Berberine inhibits COX-2 expression via the ERK1/2 signalling pathway and, possibly, at a high dosage via the JNK pathway. P38MAPK may have no relationship with the effect of BBR in PBMC. Berberine inhibited COX-2 mRNA and protein expression in a dose dependent manner and suppressed COX-2 expression to a minimal level after 12 hours of berberine treatment.

关键词： antiatherosclerotic berberine cyclooxygenase erk1/2 jnk p38mapk

Effects of aspirin on atherosclerosis and the cyclooxygenase-2 expression in atherosclerotic rabbits

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Chinese Medical Journal 2006年第21期119卷 1808-1814页

作者： GUO Yi WANG Qi-zhang TANG Bing-shan ZUO Yan-fang li fang-ming JIANG Xin WANG ling MA Ke-fu Department of Neurology Second Affiliated Hospital of Jinan University Shenzhen 518020 China Department of Neurology Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Background Atherosclerosis is a complex vascular inflammatory disease. Aspirin is a mainstay in the prevention of vascular complications of atherosclerosis. In this study, the effectiveness of aspirin in suppressing atherosclerosis and the inflammation process was evaluated in rabbits fed with a high fat diet. Methods Eighteen male New Zealand rabbits were randomly divided into 3 groups： control group, untreated cholesterol-fed group, aspirin treated cholesterol-fed group, which were fed for 12 weeks. After 12 weeks, the aorta was harvested for pathologic morphology observation. Immunohistochemical analysis of cyclooxygenase-2 （COX-2）, macrophage and vascular smooth muscle cell （VSMC） was performed. The statistical analysis was performed by the statistical program SPSS 10.0. Results The aorta plaque/intima size （P/I） by pathologic morphology observation was 0%, （59.6± 13.7）% and （36.3±16.5）% in the control, untreated cholesterol-fed group and aspirin treated group, respectively. The maximum plaque thickness, the degree of artery stenosis and the proportion of the intimal circumference occupied by atheroma of the 3 groups were significantly different from each other （P〈0.01）. The expression of COX-2 and macrophage in plaque of the aspirin treated group were decreased compared with that in untreated cholesterol-fed group. However, no difference was found in the expression of VSMC between the aspirin treated and the untreated cholesterol-fed group. Conclusion The mechanism of atherosclerosis suppression by aspirin in cholesterol-fed rabbits is related to the inhibition of COX-2 expression together with the reduced inflammation followed by, but not related to the hypolipidemic effects.

关键词： aspirin atherosclerosis cyclooxygenase-2 rabbits

The sex ratio of wild Chinese alligators Alligator sinensis

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Current Zoology 2013年第6期59卷 725-731页

作者： Lan ZHAO Hai-Qiong YANG li-ming fang Guo-liang PAN Wei-Qiang ZOU Da-Bin REN Qiu-Hong WAN Sheng-Guo fang The Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education State Conservation Center for Gene Resources of Endangered Wildlife College of Life Sciences Zhejiang University Hangzhou 310058 China Changxing Yinjiabian Chinese Alligator Nature Reserve Changxing 313100 Zhejiang China

The Chinese alligator Alligator sinensis is one of the most endangered crocodilian species, and typically exhibits temperature-dependent sex determination. It is extremely important to clarify the sex structure of Chinese alligators to implement recovery projects successfully. However, the sex ratio of wild Chinese alligators remains unknown. In this study, we collected 28 years of sex ratio data from Chinese alligators residing in the natural and artificial habitats of Changxing Nature Reserve, China, and examined the differences in the sex ratio dynamics between these two populations. We observed that the sex ratio of wild Chinese alligators is 1 male to 4.507 females, which was significantly lower compared to that of the captive population （1 to 2.040; P 〈 0.001）, and is significantly different to previously documented sex ratios for this species （all P 〈 0.01）. Furthermore, we documented an annually stable （P = 1.000） female-biased sex ratio for wild alligators at hatching [1 male to 4.747 females; 0.174 （0.167~）.182）], in contrast to a dramatically fluctuating sex ratio （P 〈 0.001） in captivity [1 male to 1.674 females; 0.374 （0.246-0.593）], showing a potential mechanism for adjusting the sex structure. Finally, we found that the hatchling sex ratios were similar to that of the population sex ratio （P = 0.748）, with little correlation to air temperature values in the 60-70 day incubation period during the breeding season （July and August; both P 〉 0.05）. Overall, this study indicates that the stabilized female-biased sex ratio of Changxing Chinese alligators might result from selection pressure caused by local mate competition and major inbreeding .

关键词： Reptile Female-biased Temperature-dependent sex determination Conservation

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竞争环境下制造商网络渠道选择演化博弈分析

软科学 2016年第8期30卷 113-118页

作者：李明芳河北科技大学经济管理学院石家庄050018

通过将演化博弈应用于竞争环境下制造商网络渠道选择研究,分析品牌竞争、渠道竞争以及顾客网络渠道接受度对制造商利润和网络渠道选择的影响作用。研究表明:竞争环境下制造商网络渠道选择是一个对称博弈,单渠道与双渠道间的利润变化会... 详细信息

通过将演化博弈应用于竞争环境下制造商网络渠道选择研究,分析品牌竞争、渠道竞争以及顾客网络渠道接受度对制造商利润和网络渠道选择的影响作用。研究表明:竞争环境下制造商网络渠道选择是一个对称博弈,单渠道与双渠道间的利润变化会影响博弈的演化趋势;品牌竞争和渠道竞争越激烈,交叉价格因子越大,双渠道带来的制造商利润增额亦越大;顾客网络渠道接受度对影响作用较为复杂,单方双渠道模式下制造商利润随着传统零售市场份额的增加而增加,双方双渠道模式下则随之而降低;博弈可能出现两种演化情况,当双渠道利润高于单渠道时趋向于双渠道,当对方单渠道的利润下降而对方双渠道的利润上升时存在两种演化稳定策略。

关键词：网络渠道选择品牌竞争渠道竞争演化博弈

Research on Jiuquan-Jiayuguan-Yumen Region's Dynamic Changing of Urbanization Based on Self-Organizing Model

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Chinese Journal of Population,Resources and Environment 2005年第3期3卷 22-28页

作者： li ming 1,2 & fang Chuanglin 2 1 Graduate School of the Chinese Academy of Sciences, Beijing 100039,China 2 Institute of Geographical Sciences and Natural Resources Research, CAS, Beijing 100101, China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Institute of Geographical Sciences and Natural Resources Research CAS Beijing 100101 China

Based on the self-organizing modeling principle in the dissipative structure theory, the paper regards the migration coupling between the regions as the influence factor to create the model of regional urbanization simulation and prediction, which is more practical to simulate the space-time and dynamic evolvement characteristics of regional urbanization level. According to the historical data about the population and urbanization of Jiuquan, Jiayuguan andYumen cities, the past 50 years' dynamic evolvement trend is simulated and the following 50 years' dynamic evolvement trend is predicted with the urbanization dynamic evolvement model based on the self-organizing model. The analysis of effective factors such as economic increase, resources exploitation, ecosystem construction, traffic location, national policies, population migration are given, then the reasons of the dynamic evolvement differences of the three cities' urbanization are drawn. The study suggests that the self-organizing model is fit for the simulation of Jiuquan-Jiayuguan-Yumen regional urbanization level in the past 50 years, and the departure between the stimulant and the actual data is under 5%, so it can be used to predict the urbanization future of the three cities. The predicted results show that the regional population will reach 1.074 million and the regional urbanization level will be 72.56%.

关键词： self-organizing urbanization dynamic evolvement Jiuquan-Jiayuguan-Yumen region

情境规范对认知失调与网购退货意愿关系的作用机理探析

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软科学 2017年第12期31卷 112-117页

作者：李明芳河北科技大学经济管理学院石家庄050018

通过将信任理论和认知失调理论应用于网购退货行为,揭示了顾客外在感知(情境规范)与内在价值认同(退货政策认可、产品熟悉)的交互调节机制,探讨情境规范对认知失调与退货意愿关系的调节作用、退货政策关注对调节作用的中介效应、以及产... 详细信息

通过将信任理论和认知失调理论应用于网购退货行为,揭示了顾客外在感知(情境规范)与内在价值认同(退货政策认可、产品熟悉)的交互调节机制,探讨情境规范对认知失调与退货意愿关系的调节作用、退货政策关注对调节作用的中介效应、以及产品熟悉度的再次调节效应。研究表明情境规范负向调节认知失调与退货意愿的正向关系;退货政策认可有利于弱化认知失调对退货意愿的积极作用,同时中介情境规范对二者关系的调节效应;顾客的产品熟悉度会再次调节情境规范的调节效应,当顾客对产品熟悉度高时,情境规范对认知失调与退货意愿关系的调节作用显著,反之不显著。

关键词：产品退货认知失调退货意愿情境规范