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Production of Transgenic Anliucheng Sweet Orange(Citrus sinensis Osbeck) with Xa21 Gene for Potential Canker Resistance

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Journal of Integrative Agriculture 2014年第11期13卷 2370-2377页

作者： li ding-li XIAO Xuan GUO Wen-wu Key Laboratory of Horticultural Plant Biology Ministry of Education/College of Horticulture & Forestry Sciences Huazhong Agricultural University College of life and Environmental Sciences Gannan Normal University

Citrus canker, an epidemic quarantine disease caused by Xanthomonas axonopodis pv. citri, has brought a great damage in citrus production worldwide. Herein, a rice PRR （pattern recognition receptor） gene Xa21 together with GUS reporter gene and hygromycin phosphotransferase gene （HPT） was introduced into Anliucheng sweet orange （Citrus sinensis Osbeck） via Agrobacterium-mediated transformation of embryogenic callus. The transgenic calluses were screened on MT basal medium containing hygromycin （HYG） and detected by histochemical GUS staining. The transgenic plantlets were recovered through somatic embryogenesis pathway. The regenerated plantlets were accustomed to and maintained in the greenhouse. The transgene integration of recovered plantlets was identiifed by PCR and Southern blot hybridization. It showed that all the transgenic plantlets tested had undergone single copy integration, the expression of Xa21 in eight different transgenic lines detected by qRT-PCR can be divided into three grades, high for T5 and T6, middle for T4 and low for the rest. The tolerance to citrus canker disease of the three recovered transgenic lines T2, T4 and T6 was assessed by in vitro pin-puncture inoculation. The results showed that all the three transgenic lines conferred improved resistance to citrus canker bacterium infection and the T4 transgenic line displayed the highest resistance. The mechanism and feasibility of rice Xa21 in triggering innate immunity in citrus was brielfy discussed.

关键词： citrus canker Xa21 sweet orange pattern recognition receptors (PRRs)

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The formation and tectonic evolution of Philippine Sea Plate and KPR

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Acta Oceanologica Sinica 2011年第4期30卷 75-88页

作者： FANG Yinxia li Jiabiao li Mingbi ding Weiwei ZHANG Jie The Second Institute of Oceanography State Oceanic Administration Hangzhou 310012 China

The Philippine Sea Plate has an extremely special tectonic background. As an oceanic plate,it is almost entirely surrounded by subduction zones with complex internal tectonic features. On the basis of enormous published literature,this paper offers a comprehensive overview of the tectonic and evolution history of the Philippine Basin and the Kyushu-Palau Ridge（KPR） in the Philippine Sea Plate,and discusses the geological features of KPR. Referring to relevant definitions of various ＂ridges＂ stipulated in United Nations Convention on the Law of the Sea,so the KPR is believed to be a remnant arc formed during the opening of the Parece Vela and Shikoku Basins in the Philippine Sea Plate. It is a submarine ridge on oceanic plate rather than a submarine elevation. And thus,it is not a natural component of the Japan continental margin.

关键词： Philippine Sea Plate West Philippine Basin KPR submarine ridge

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A machine learning approach to TCAD model calibration for MOSFET

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Nuclear Science and Techniques 2023年第12期34卷 133-145页

作者： Bai‑Chuan Wang Chuan‑Xiang Tang Meng‑Tong Qiu Wei Chen Tan Wang Jing‑Yan Xu li‑li ding Department of Engineering Physics Tsinghua UniversityBeijing 100084China Key Laboratory of Particle and Radiation Imaging(Tsinghua University) Ministry of EducationBeijing 100084China State Key Laboratory of Intense Pulsed Radiation Simulation and Effect Xi’an 710024China

Machine learning-based surrogate models have significant advantages in terms of computing efficiency. In this paper, we present a pilot study on fast calibration using machine learning techniques. Technology computer-aided design(TCAD) is a powerful simulation tool for electronic devices. This simulation tool has been widely used in the research of radiation ***, calibration of TCAD models is time-consuming. In this study, we introduce a fast calibration approach for TCAD model calibration of metal–oxide–semiconductor field-effect transistors(MOSFETs). This approach utilized a machine learning-based surrogate model that was several orders of magnitude faster than the original TCAD simulation. The desired calibration results were obtained within several seconds. In this study, a fundamental model containing 26 parameters is introduced to represent the typical structure of a MOSFET. Classifications were developed to improve the efficiency of the training sample generation. Feature selection techniques were employed to identify important parameters. A surrogate model consisting of a classifier and a regressor was built. A calibration procedure based on the surrogate model was proposed and tested with three calibration goals. Our work demonstrates the feasibility of machine learning-based fast model calibrations for MOSFET. In addition, this study shows that these machine learning techniques learn patterns and correlations from data instead of employing domain expertise. This indicates that machine learning could be an alternative research approach to complement classical physics-based research.

关键词： Machine learning Radiation effects Surrogate model TCAD model calibration

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Effect of electroacupuncture on the mRNA and protein expression of Rho-A and Rho-associated kinase Ⅱ in spinal cord injury rats

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Neural Regeneration Research 2017年第2期12卷 276-282页

作者： You-jiang Min li-li-qiang ding li-hong Cheng Wei-ping Xiao Xing-wei He Hui Zhang Zhi-yun Min Jia Pei Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine Nanchang Jiangxi Province China Shanghai University of Traditional Chinese Medicine Shanghai China

Electroacupuncture is beneficial for the recovery of spinal cord injury, but the underlying mechanism is unclear. The Rho/Rho-associated kinase（ROCK） signaling pathway regulates the actin cytoskeleton by controlling the adhesive and migratory behaviors of cells that could inhibit neurite regrowth after neural injury and consequently hinder the recovery from spinal cord injury. Therefore, we hypothesized electroacupuncture could affect the Rho/ROCK signaling pathway to promote the recovery of spinal cord injury. In our experiments, the spinal cord injury in adult Sprague-Dawley rats was caused by an impact device. Those rats were subjected to electroacupuncture at Yaoyangguan（GV3）, Dazhui（GV14）, Zusanli（ST36） and Ciliao（BL32） and/or monosialoganglioside treatment. Behavioral scores revealed that the hindlimb motor functions improved with those treatments. Real-time quantitative polymerase chain reaction, fluorescence in situ hybridization and western blot assay showed that electroacupuncture suppressed the m RNA and protein expression of Rho-A and Rho-associated kinase Ⅱ（ROCKⅡ） of injured spinal cord. Although monosialoganglioside promoted the recovery of hindlimb motor function, monosialoganglioside did not affect the expression of Rho-A and ROCKⅡ. However, electroacupuncture combined with monosialoganglioside did not further improve the motor function or suppress the expression of Rho-A and ROCKⅡ. Our data suggested that the electroacupuncture could specifically inhibit the activation of the Rho/ROCK signaling pathway thus partially contributing to the repair of injured spinal cord. Monosialoganglioside could promote the motor function but did not suppress expression of Rho A and ROCKⅡ. There was no synergistic effect of electroacupuncture combined with monosialoganglioside.

关键词： nerve regeneration spinal cord injury electroacupuncture Rho/Rho-associated kinase signaling pathway monosialoganglioside motor function cytoskeleton real-time quantitative polymerase chain reaction western blot assay hybridization in situ neural regeneration

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Phenotype and mechanism analysis of plant dwarfing in pear regulated by abscisic acid

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Journal of Integrative Agriculture 2022年第5期21卷 1346-1356页

作者： liU Jian-long ZHANG Chen-xiao li Tong-tong liANG Cheng-lin YANG Ying-jie li ding-li CUI Zhen-hua WANG Ran SONG Jian-kun College of Horticulture Qingdao Agricultural UniversityQingdao 266109P.R.China Haidu College Qingdao Agricultural UniversityLaiyang 265200P.R.China

Close planting of dwarf varieties is currently the main cultivation direction for pear trees,and the screening of excellent dwarf varieties is an important goal for *** this study,the dwarfing pear variety‘601D’and its vigorous mutant‘601T’were used to show their biological characteristics and further explore the dwarfing mechanism in‘601D’.The biological characteristics showed that‘601D’had a shorter internode length,a shorter and more compact tree body,thicker and broader leaves,lower stomata density,larger stomata size(dimension),and higher photosynthetic *** biological characteristics of‘601T’showed notable *** results of endogenous hormone tests indicated that the contents of abscisic acid(ABA),ABA-glucosyl ester,and GA_(4) were higher in‘601D’,but the trans-zeatin content was *** transcriptomic analysis,significant differences were found in the biosynthetic and metabolic pathways of *** transcription factors such as bHLH,WRKY,and homeobox also participated in the regulation of plant *** therefore examined three hormones with obvious differences with‘601T’,and found that only ABA could induce‘601T’to return to a dwarfing plant ***,we conclude that the dwarfing of‘601D’is caused by an excessive accumulation of *** study provides a new theoretical basis for breeding dwarf varieties.

关键词： dwarf pear phenotype abscisic acid transcriptomic analysis

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Different phenotypes of monocytes in patients with newonset mild acute pancreatitis

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World Journal of Gastroenterology 2017年第8期23卷 1477-1488页

作者： Man-li Zhang Yan-Fang Jiang Xin-Rui Wang li-li ding Hong-Juan Wang Qing-Qing Meng Pu-Jun Gao Department of Hepatology and Gastroenterology The Second Part of First Hospital of Jilin University Department of Central Laboratory The Second Part of First Hospital of Jilin University Key Laboratory of Zoonosis Research Ministry of EducationJilin University Department of Hepatology The First Hospital of Jilin University Department of Intensive Care Unit The First Hospital of Jilin University

AIM To evaluate the numbers of different subsets of monocytes and their associations with the values of clinical measures in mild acute pancreatitis(MAP) *** The study included one group of 13 healthy controls and another group of 24 patients with new-onset MAP. The numbers of different subsets of monocytes were examined in these two groups of subjects by flow cytometry. The concentrations of plasma interleukin(IL)-10 and IL-12 were determined by cytometric bead array. The acute physiology and chronic health evaluation(APACHE) II scores of individual patients were evaluated, and the levels of plasma C-reactive protein(CRP) as well as the activities of amylase and lipase were measured. RESULTS In comparison with that in the controls, significantly increased numbers of CD14+CD163-, CD14+CD163-MAC387+ M1 monocytes, but significantly reduced numbers of CD14+CD163+IL-10+ M2 monocytes were detected in the MAP patients(P < 0.01 or P < 0.05). Furthermore, significantly higher levels of plasma IL-10 and IL-12 were observed in the MAP patients(P < 0.01 for all). More importantly, the levels of plasma CRP were positively correlated with the numbers of CD14+CD163-(R = 0.5009, P = 0.0127) and CD14+CD163-MAC387+(R = 0.5079, P = 0.0113) M1 monocytes and CD14+CD163+CD115+ M2 monocytes(R = 0.4565, P = 0.0249) in the patients. The APACHE II scores correlated with the numbers of CD14+CD163+CD115+(R = 0.4581, P = 0.0244) monocytes and the levels of plasma IL-10(R = 0.4178, P = 0.0422) in the MAP patients. However, there was no significant association among other measures tested in this population. CONCLUSION Increased numbers of CD14+CD163- and CD14+ CD163-MAC387+ monocytes may contribute to the pathogenesis of MAP, and increased numbers of CD14+CD163+CD115+ monocytes may be a biomarker for evaluating the severity of MAP.

关键词： Mild acute pancreatitis Monocyte Cytokine Acute physiology and chronic health evaluation II score C-reactive protein

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Impact of neutron-induced displacement damage on the single event latchup sensitivity of bulk CMOS SRAM

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Chinese Physics B 2017年第1期26卷 542-546页

作者： Xiao-Yu Pan Hong-Xia Guo Yin-Hong Luo Feng-Qi Zhang li-li ding Jia-Nan Wei Wen Zhao Northwest Institute of Nuclear Technology Xi＇an 710024 China

Since the displacement damage induced by the neutron irradiation prior has negligible impact on the performance of the bulk CMOS SRAM, we use the neutron irradiation to degrade the minority carrier lifetime in the regions responsible for latchup. With the experimental results, we discuss the impact of the neutron-induced displacement damage on the SEL sensitivity and qualitative analyze the effectiveness of this suppression approach with TCAD simulation.

关键词： displacement damage neutron irradiation single event latchup TCAD simulation

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Chromosome-level genome assembly of the Asian citrus psyllid,Diaphorina citri

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Insect Science 2024年第1期31卷 13-27页

作者： Shuang Lei Shi-Jiang Yu Qi Pan li-li ding Si-Chen li Lu-Yan Cheng Shu-Qi Wang Bing-Hai Lou Jun He Cui-Yun Lei lin Cong Hao-Qiang liu Xue-Feng Wang Chun Ran Citrus Research Institute Southwest University/Chinese Academy of Agricultural SciencesNational Engineering Research Center for CitrusChongqingChina

Diaphorina citri is a global citrus *** a vector insect,it can transmit the causative agents of citrus huanglongbing,causing irreversible losses to the citrus *** acquisition of genomic information can provide a molecular genetic basis for effective control of ***,the DNBSEQ™,Oxford Nanopore Technologies,and Hi-C technologies are applied to generate a high-quality chromosome-level genome of *** genome size of *** was 523.78 Mb with a scaffold N50 of 47.05 Mb distributed on 13 chromosomes.A total of 250.64 Mb(47.85%)repeat sequences and 24048 protein-coding genes were *** resequencing of female and male individuals indicated that the sex chromosome system of *** is *** analysis demonstrated that *** and Pachypsylla venusta,which separated from their most recent common ancestor about 336.62 million years ago,were the most closely ***,we identified genes potentially involved in detoxification metabolism,pathogen transmission,and honeydew secretion for further *** high-quality genome provides an important reference for developing effective management strategies of ***.

关键词： Asian citrus psyllid chromosome-level genome comparative genomics cytochrome P450 detoxification Huanglongbing

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Alterations in serotonin, transient receptor potential channels and protease-activated receptors in rats with irritable bowel syndrome attenuated by Shugan decoction

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World Journal of Gastroenterology 2015年第16期21卷 4852-4863页

作者： Hai-lian Shi Chu-Hsuan liu li-li ding Yu Zheng Xiao-Yan Fei Lu Lu Xue-Ming Zhou Jian-Ye Yuan Jian-Qun Xie Institute of Digestive Diseases Longhua HospitalShanghai University of Traditional Chinese Medicine Institute of Chinese Materia Medica Shanghai Key Laboratory of Complex PrescriptionShanghai University of Traditional Chinese Medicine Experimental Animal Center Shanghai University of Traditional Chinese Medicine

AIM:To determine the molecular mechanisms of Shugan decoction(SGD) in the regulation of colonic motility and visceral hyperalgesia(VHL) in irritable bowel syndrome(IBS).METHODS:The chemical compounds contained in SGD were measured by high-performance liquid chromatography.A rat model of IBS was induced by chronic water avoidance stress(WAS).The number of fecal pellets was counted after WAS and the pain pressure threshold was measured by colorectal *** changes in colonic mucosa were detected by hematoxylin-eosin *** contents of tumor necrosis factor(TNF)-αin colonic tissue and calcitonin-gene-related peptide(CGRP)in serum were measured by *** protein expression of serotonin[5-hydroxytryptamide(5-HT)],serotonin transporter(SERT),chromogranin A(Cg A)and CGRP incolon tissue was measured by ***:SGD inhibited colonic motility dysfunction and VHL in rats with *** of transient receptor potential(TRP)vanilloid 1(TRPV1)(Ruthenium Red)and TRP ankyrin-1(TRPA1)(HC-030031)and activator of protease-activated receptor(PAR)4 increased the pain pressure threshold,whereas activators of PAR2and TRPV4 decreased the pain pressure threshold in rats with *** effect of SGD on pain pressure threshold in these rats was abolished by activators of TRPV1(capsaicin),TRPV4(RN1747),TRPA1(Polygodial)and PAR2(AC55541).In addition,CGRP levels in serum and colonic tissue were both increased in these ***-αlevel in colonic tissue was also significantly ***,the levels of 5-HT,SERT and Cg A in colonic tissue were *** these pathological changes in rats with IBS were attenuated by ***:SGD alleviated VHL and attenuated colon motility in IBS,partly by regulating TRPV1,TRPV4,TRPA1,PAR2,5-HT,Cg A and SERT,and reducing CGRP and TNF-αlevel.

关键词： Shugan decoction Visceral hyperalgesia Serotonin Transient receptor potential Proteaseactivatedreceptor Serotonin transporter Calcitoningene-related peptide Tumor necrosis factor-α

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新型波长解调技术在干涉型光纤传感器输出解调中的应用

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光谱学与光谱分析 2017年第9期37卷 2948-2953页

作者：陈宸李仙丽丁晖西安交通大学电力设备电气绝缘国家重点实验室陕西西安710049

提出了一种改进型边缘滤波波长解调技术,解决干涉型或谐振型光纤传感器输出的干涉谱波长的快速解调问题。该波长解调技术基于啁啾光栅和长周期光栅的光谱特点,以啁啾光栅作为矩形滤波器,提取传感器输出干涉谱的一个干涉峰;以长周期作为... 详细信息

提出了一种改进型边缘滤波波长解调技术,解决干涉型或谐振型光纤传感器输出的干涉谱波长的快速解调问题。该波长解调技术基于啁啾光栅和长周期光栅的光谱特点,以啁啾光栅作为矩形滤波器,提取传感器输出干涉谱的一个干涉峰;以长周期作为边缘滤波器,将干涉峰的波长信息转化为光强信息进行检测。该波长解调技术波长解调范围为传感器输出干涉谱的一个自由光谱范围(FSR),解调速度快,克服了当前波长解调技术中存在的测量范围与测量速度难以同时提高的问题,同时还具有系统成本低,体积小,能够灵活多路扩展的特点。详细介绍了解调系统的工作原理以及系统结构,并将其实际应用于一种干涉型光纤电流传感器输出干涉谱的波长解调中。通过实验证明电流传感器工作在工频及脉冲电流(脉冲宽度25μs)情况下,该解调系统对传感器输出干涉谱具有准确、快速的解调效果。该研究对推进干涉或谐振型光纤传感器的实际工程化水平的提高具有重要意义。

关键词：波长解调边缘滤波法干涉型光纤传感器啁啾光栅长周期光栅

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