Alterations in serotonin, transient receptor potential channels and protease-activated receptors in rats with irritable bowel syndrome attenuated by Shugan decoction

作     者：Hai-Lian Shi Chu-Hsuan Liu Li-Li Ding Yu Zheng Xiao-Yan Fei Lu Lu Xue-Ming Zhou Jian-Ye Yuan Jian-Qun Xie 

作者机构：Institute of Digestive DiseasesLonghua HospitalShanghai University of Traditional Chinese Medicine Institute of Chinese Materia MedicaShanghai Key Laboratory of Complex PrescriptionShanghai University of Traditional Chinese Medicine Experimental Animal CenterShanghai University of Traditional Chinese Medicine 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2015年第21卷第16期

页      面：4852-4863页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Supported by Innovation Program of the Shanghai Municipal Education Commission,No.12YZ065 National Natural Science Foundation of China,No.81072786,No.81473630 and No.81202665 Longhua Medical Project,No.D-09 High level Project of the University of Educational Commission of Shanghai,China,No.2008GSP19 Shanghai Leading Academic Discipline Project,No.J50305 

主　　题：Shugan decoction Visceral hyperalgesia Serotonin Transient receptor potential Proteaseactivatedreceptor Serotonin transporter Calcitoningene-related peptide Tumor necrosis factor-α 

摘      要：AIM:To determine the molecular mechanisms of Shugan decoction(SGD) in the regulation of colonic motility and visceral hyperalgesia(VHL) in irritable bowel syndrome(IBS).METHODS:The chemical compounds contained in SGD were measured by high-performance liquid chromatography.A rat model of IBS was induced by chronic water avoidance stress(WAS).The number of fecal pellets was counted after WAS and the pain pressure threshold was measured by colorectal *** changes in colonic mucosa were detected by hematoxylin-eosin *** contents of tumor necrosis factor(TNF)-αin colonic tissue and calcitonin-gene-related peptide(CGRP)in serum were measured by *** protein expression of serotonin[5-hydroxytryptamide(5-HT)],serotonin transporter(SERT),chromogranin A(Cg A)and CGRP incolon tissue was measured by ***:SGD inhibited colonic motility dysfunction and VHL in rats with *** of transient receptor potential(TRP)vanilloid 1(TRPV1)(Ruthenium Red)and TRP ankyrin-1(TRPA1)(HC-030031)and activator of protease-activated receptor(PAR)4 increased the pain pressure threshold,whereas activators of PAR2and TRPV4 decreased the pain pressure threshold in rats with *** effect of SGD on pain pressure threshold in these rats was abolished by activators of TRPV1(capsaicin),TRPV4(RN1747),TRPA1(Polygodial)and PAR2(AC55541).In addition,CGRP levels in serum and colonic tissue were both increased in these ***-αlevel in colonic tissue was also significantly ***,the levels of 5-HT,SERT and Cg A in colonic tissue were *** these pathological changes in rats with IBS were attenuated by ***:SGD alleviated VHL and attenuated colon motility in IBS,partly by regulating TRPV1,TRPV4,TRPA1,PAR2,5-HT,Cg A and SERT,and reducing CGRP and TNF-αlevel.