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Generation of Hematopoietic Stem Cells from Purified Embryonic Endothelial Cells by a Simple and Efficient Strategy

Journal of Genetics and Genomics 2013年第11期40卷 557-563页

作者： zhuan li Fan Zhou Dongbo Chen Wenyan He Yanli Ni lingfei Luo Bing liu Key Laboratory of Freshwater Fish Reproduction and Development Ministry of EducationLaboratory of Molecular Developmental BiologySchool of Life ScienceSouthwest University 307-Ivy Translational Medicine Center Laboratory of OncologyAffiliated Hospital of Academy of Military Medical Sciences

Recent progress by versatile approaches supports the new hypothesis that multi-potent hematopoietic stein cells （HSCs） are directly formed from a rare population of endothelial cells in mid-gestation mouse embryos. This process is therefore known as the endothelial-to- hematopoietic transition （EHT）. Nevertheless, there is no functional evidence that documents the HSC transition from purified endothelial cells. In this study, we developed an OP9-DLl-based co-culture system that was able to facilitate the HSC specification and/or expansion in vitro of mouse embryonic day 10.5 （El0.5） Tie2~ cells remarkably. Then, the immunophenotypically defined endothelial ceils were harvested by a combination of surface markers （Flkl＋CD31 ~CD41 CD45 Ter119 ） from the caudal half of EI0.0-EI 1.0 mouse embryos. The transplantation of the endothelia/OP9-DL1 co-cultures led to long-term, high-level, multi-lineage, and multi-organ he- matopoietic reconstitution in the irradiated adult recipients. The induced HSC activity was initially observed at El0.5, and a significant increase was detected at El 1.0, which suggests a temporally specific regulation. Taken together, tbr the first time, we provide functional evidence showing the HSC potential of purified embryonic endothelial cells, which is indispensable for the emerging EHT concept. Moreover, the newly defined co-culture system will aid the exploration of the key molecules governing the HSC transition from embryonic and even postnatal endothelial cells, which has enormous significance in basic and translational research.

关键词： Hematopoietic stem cells Endothelial-to-hematopoietic transition Endothelial cells Aorta-gonad--mesonephros region

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Multi-phasic CT arterial portography and CT hepatic arteriography improving the accuracy of liver cancer detection

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World Journal of Gastroenterology 2004年第21期10卷 3118-3121页

作者： lili li-Zhiliu zhuan-MiaoXie Yun-XianMo lieZheng Chao-MeiRuan linChen Pei-HongWu ImagingDiagnosis&InterventionalCenter CancerCenterSunYat-senUniversity.651DongfengRoadEastGuangzhou510060.GuangdongProvinceChina

AIM: To evaluate the value of multi-phasic CT arterial portography (CTAP) and CT hepatic arteriography (CTHA) in differential diagnosis of liver diseases, and to improve the specificity of CTAP and CTHA for liver cancerdetection. METHODS: From January 1999 to December 2002, multiphasic CTAP and CTHA were performed in 20 patients with suspected liver disease. CT scanning was begun 25s, 60s and 120s for the early-, late- and delayed-phase CTAP examinations, and 6sec, 40 s and 120 s for the early-, lateand delayed-phase CTHA examinations respectively, after a transcatheter arterial injection of non-ionic contrast material. If a lesion was diagnosed as a liver cancer, transcatheter hepatic arterial chemoembolization (TACE) treatment was performed, and the follow-up CT was performed three or four weeks ***: All eighteen HCCs in 12 cases were shown as nodular enhancement on early-phasic CTHA. The density of the whole tumor decreased rapidly on late and delayed phases, and the edge of 12 tumors (12/18) remained relatively hyperdense compared with the surrounding liver tissue, and demonstrated as rim enhancement. All HCCs were shown as perfusion defect nodules on multi-phasic CTAP. Five tumors (5/18) were shown as rim enhancement on delayed-phasic CTAP. Rim enhancement was shown as 1 to 2-mm-wide irregular, uneven and discontinuous circumferential enhancement at late-, and delayed-phase of CTHA or CTAP. Five pseudolesions and 4 hemoangiomas were found in multi-phasic CTAP and CTHA. No pseudolesions and hemoangiomas were shown as rim enhancement on late- or delayed-phasic CTHA and ***: Multi-phasic CTAP and CTHA could help to recognize the false-positive findings in CTAP and CTHA images, and improve the accuracy of CTAP and CTHA of liver cancer detection.

关键词：多形CT 门静脉造影术动脉肝动脉精确性肝癌肿瘤 CTHA 消化系统

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Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats

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Journal of Integrative Agriculture 2013年第1期12卷 118-126页

作者： LAN Xian-yong ZHAO Hai-yu li zhuan-jian ZHOU Rui PAN Chuan-ying LEI Chu-zhao CHEN Hong College of Animal Science and Technology Northwest A&F University/Shaanxi Key Laboratory of Molecular Biology for Agriculture College of life Sciences Northwest A&F University

Paired-like homeodomain transcription factor 1 （PITX1） plays an important role in pituitary development by indirectly regulating the expression of the GH and PRL genes, and therefore PITX1 gene is regarded as a potential candidate gene for building the relationship between the gene polymorphism and milk traits. The aim of this study was to explore the novel genetic variant in PITX1 gene and its effect on milk performance in dairy goats. Herein, a novel genetic variation （NW_00314033： g.201GA or IVS1＋41GA） located at nt41 position of the first intron of the goat PITX1 gene was reported at the P1 locus, which can be genotyped by the Msp I PCR-RFLP. In the Msp I PCR-RFLP analyis, the GG variant was a major genotype, and the A variant was a minor allele in Guanzhong dairy goats which was at Hardy-Weinberg disequilibrium （chi-square χ2=140, P0.01）. The establishment of associations between different genotypes and milk performance was performed in the analyzed population. A total of three significant associations of the polymorphism with average milk fat content （%）（P=0.045）, morning milk fat content （%）（P=0.049）, and afternoon milk fat content （%）（P=0.050）, were found, respectively. A significant relationship between the polymorphism and average total solid content （P=0.029） was also detected. This novel single nucleotide polymorphism （SNP） extended the spectrum of genetic variation of the goat PITX1 gene, and its significant association with milk performance would benefit from the application of DNA markers related to improving milk performance through marker-assisted selection （MAS） in dairy goats.

关键词： dairy goat PITX1 gene genetic variant association milk performance

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南水北调东线泵站机组站内叶片全调节优化研究

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水利学报 2016年第10期47卷 1332-1338页

作者：李伟专祥涛武汉大学动力与机械学院湖北武汉430072

南水北调工程是解决我国北方水资源缺乏的重大工程,而淮安四站是南水北调东线工程泵站之一。本文在考虑分时电价的基础上研究淮安四站的运行优化问题,建立泵站机组费用最小的叶片全调节数学模型,并针对该模型特点,提出了一种改进的动态... 详细信息

南水北调工程是解决我国北方水资源缺乏的重大工程,而淮安四站是南水北调东线工程泵站之一。本文在考虑分时电价的基础上研究淮安四站的运行优化问题,建立泵站机组费用最小的叶片全调节数学模型,并针对该模型特点,提出了一种改进的动态规划求解算法。最后,利用算例验证了该算法的可行性。计算结果表明:(1)在设计扬程、机组满负荷工作情况下,叶片全调节的调度方案与叶片角额定安放角度相比,可节约费用4.88%。(2)机组能源经济效率受叶片角和流量负荷转移两个因素影响:其中叶片角与机组自身的特性曲线相关,当扬程较大时,叶片角影响较小;流量负荷转移与机组容量与需求量相关,机组容量越大,负荷转移越显著。当需求量较小时,叶片角是主要影响因素,当需求量较大时,流量负荷转移是主要影响因素。

关键词：南水北调运行优化动态规划能源经济效率

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Reduced corporal fibrosis to protect erectile function by inhibiting the Rho-kinase/liM-kinase/ cofilin pathway in the aged transgenic rat harboring human tissue kallikrein 1

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Asian Journal of Andrology 2017年第1期19卷 67-72页

作者： Kai Cui Yang Luan Tao Wang li zhuan Ke Rao Shao-Gang Wang Zhang-Qun Ye Ji-Hong liu Dao-Wen Wang Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Institute of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Reproductive Medicine the First People's Hospital of Yunnan Province Kunming 650032 China Division of Cardiology Department of Internal Medicine Tengji Hospiial Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China.

Our previous studies have demonstrated that erectile function was preserved in aged transgenic rats （TGR） harboring the human tissue kallikrein 1 （hKLK1）, while the molecular level of hKLK1 on corporal fibrosis to inhibit age-related erectile dysfunction （ED） is poorly understood. Male wild-type Sprague-Dawley rats （WTR） and TGR harboring the hKLK1 gene were fed to 4- or 18-month-old and divided into three groups： young WTR （yWTR） as the control, aged WTR （aWTR）, and aged TGR （aTGR）. Erectile function of all rats was assessed by cavernous nerve electrostimulation method. Masson＇s trichrome staining was used to evaluate corporal fibrosis in the corpus cavernosum. We found that the erectile function of rats in the aWTR group was significantly lower than that of other two groups. Masson＇s trichrome staining revealed that compared with those of the yWTR and aTGR groups, the ratio of smooth muscle cell （SMC）/collagen （C） was significantly lower in the aWTR group. Immunohistochemistry and Western blotting analysis were performed, and results demonstrated that expression of a-SMA was lower, while expressions of transforming growth factor-β1 （TGF-β1）, RhoA, ROCK1, p-MYPT1, p-liMK2, and p-cofilin were higher in the aWTR group compared with those in other two groups. However, liMK2 and cofilin expressions did not differ among three groups. Taken together, these results indicated that the RhoA/ROCK1/liMK/cofilin pathway may be involved in the corporal fibrosis caused by advanced age, and hKLK1 may reduce this corporal fibrosis by inhibiting the activation of this pathway to ameliorate age-related ED.

关键词： aged corporal fibrosis erectile dysfunction gene therapy hKLK1

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Global, regional, and national burden of gallbladder and biliary diseases from 1990 to 2019

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World Journal of Gastrointestinal Surgery 2023年第11期15卷 2564-2578页

作者： Zhong-zhuan li lin-Jing Guan Rong Ouyang Zhi-Xin Chen Guo-Qing Ouyang Hai-Xing Jiang Department of Gastroenterology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of Gastroenterology Liuzhou Workers’Hospital(The Fourth Affiliated Hospital of Guangxi Medical University)Liuzhou 545007Guangxi Zhuang Autonomous RegionChina Department of Abdomen Ultrasound Nanning Sixth People’s HospitalNanning 530002Guangxi Zhuang Autonomous RegionChina Department of General Surgery Liuzhou People’s Hospital Affiliated to Guangxi Medical UniversityLiuzhou 545006Guangxi Zhuang Autonomous RegionChina

BACKGROUND Gallbladder and biliary diseases(GABDs)are a major public health *** To analysis the cause-specific incidence,prevalence,and years lived with disability(YLDs)and its temporal trends of GABDs at the global,regional,and national *** on GABD were available from the Global Burden of Disease study *** The estimated annual percentage change(EAPC)was used to quantify temporal trend in GABD age-standardized incidence rates(ASIRs),age-standardized prevalence rate(ASPR),and age-standardized YLD rate(ASYR)by region,*** analyzed the relationship between the GABD burden and country development level using the human development index(HDI).RESULTS In 2019,the incident cases of GABD were 52003772,with an ASIR of 63432/100000 ***,the number of incident cases and ASIR of GABD increased 97%and 58.9%between 1990 and ***,the ASPR and ASYR decreased from 1990 to 2019,the number of prevalent and YLDs cases *** highest ASIR was observed in Italy,and the highest ASPR and ASYR was observed in United *** highest burden of GABD was found in low-SDI region,and the burden in female was significantly higher than males.A generally negative correlation(ρ=-0.24,Plic health challenge;however,the burden of GABD varies ***,the number of incident cases and ASIR of GABD increased between 1990 and *** results of our study provide insight into the global disease burden of GABD and may assist policymakers in formulating effective policies to mitigate modifiable risk factors.

关键词： Gallbladder and biliary diseases,incidence,prevalence,years lived with disability The Global Burden of Diseases study Estimated annual percentage changes

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Chemical vapor deposition synthesis of two-dimensional freestanding transition metal oxychloride for electronic applications

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Science China(Information Sciences) 2019年第12期62卷 149-163页

作者： Shengnan YAN Pengfei WANG Chen-Yu WANG Tao XU zhuan li Tianjun CAO Moyu CHEN Chen PAN Bin CHENG litao SUN Shi-Jun liANG Feng MIAO National Laboratory of Solid State Microstructures School of PhysicsCollaborative Innovation Center of Advanced Microstructures Nanjing University Key Laboratory of MEMS of Ministry of Education Southeast University

Two-dimensional transition metal oxychlorides(MOCl, M = Fe, Cr, V, Ti, Sc) with the metaloxygen plane sandwiched by two layers of chloride ions possess many exotic physical properties. Nevertheless,it is of great challenge to grow two-dimensional single-crystal MOCl because polyvalent nature of transition metal elements usually gives rise to mixed oxyhalides compounds with distinct physical properties. Here, we take VOCl as an example to present a solution for synthesizing 2 D freestanding MOCl with various thicknesses through chemical vapor deposition(CVD) method. The single crystal and elementary composition as well as elements ratio of as-grown samples have been characterized through measurements of X-ray diffraction, X-ray photoelectron spectroscopy and energy-dispersive spectroscopy, respectively. Furthermore, we demonstrate that 2 D VOCl-based memristive devices show low power consumption and excellent device reliability due to the layered-structure and electrically insulating properties of 2 D VOCl flakes. Besides, we utilize the feature of multilevel resistive switching that memristive devices exhibit to emulate depression and potentiation of synaptic plasticity. This method developed in this study may open up a new avenue for the growth of 2 D MOCl with single crystal and pave the way for high-performance electronic applications.

关键词： transition metal oxychlorides chemical vapor deposition freestanding memristive device neuromorphic computing

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Integrative analysis of hypothalamic transcriptome and genetic association study reveals key genes involved in the regulation of egg production in indigenous chickens

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Journal of Integrative Agriculture 2022年第5期21卷 1457-1474页

作者： WANG Dan-dan ZHANG Yan-yan TENG Meng-lin WANG Zhang XU Chun-lin JIANG Ke-ren MA Zheng li zhuan-jian TIAN Ya-dong KANG Xiang-tao li Hong liU Xiao-jun College of Animal Science and Technology Henan Agricultural UniversityZhengzhou 450046P.R.China Henan Key Laboratory for Innovation and Utilization of Chicken Germ plasm Resources Zhengzhou 450046P.R.China International Joint Research Laboratory for Poultry Breeding of Henan Zhengzhou 450046P.R.China School of life Science and Engineering Foshan UniversityFoshan 528225P.R.China

Indigenous chicken products are increasingly favored by consumers due to their unique meat and egg ***,the relatively poor egg-laying performance largely impacts the economic benefits and hinders sustainable development of the local chicken ***,excavating key genes and effective molecular markers associated with egg-laying performance is necessary to improve egg production via genetic selection in indigenous *** the present study,comparative hypothalamic transcriptome between pre-laying(15 weeks old)and peak-laying(30 weeks old)Lushi blueshelled-egg(LBS)chicken was performed.A total of 518 differentially expressed genes(DEGs)were *** the DEGs,64 genes were enriched in 10 Gene Ontology(GO)terms associated with reproductive regulation via GO analysis and considered as potential candidate genes regulating egg-laying *** the 64 genes,16 showed high connectivity(degree≥12)by protein–protein interaction(PPI)network analysis and were considered as potential core candidate genes(PCCGs).To further look for key candidate genes from the PCCGs,firstly,the expression patterns of the 16 genes were examined in the hypothalamus of two indigenous breeds(LBS and Gushi(GS)chickens)between the pre-laying and peak-laying stages using quantitative real-time PCR(qRT-PCR).Eleven out of the 16 genes showed significantly differential expression(P<0.05)with the same changing trends in the two ***,correlations between the expression levels of the above 11 genes and egg numbers and reproductive hormone concentrations in serum were investigated in high-yielding and low-yielding GS *** the 11 genes,eight showed significant correlations(P<0.05)between their expression levels and egg numbers,and between expression levels and reproductive hormone concentration in ***,an association study on single nucleotide polymorphisms(SNPs)identified in these eight genes and egg production traits was carried out in 640 GS hens,and

关键词： hypothalamus RNA-seq egg production SNP indigenous chicken

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Aptamer/polydopamine nanospheres complex based fluorescent biosensor for sensitive and selective detection of ochratoxin A

Aptamer/polydopamine nanospheres complex based fluorescent b...

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中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： li-zhuan Zhang Xu-Hua Zhao Department of Biochemistry and Molecular Biology Shanxi Medical University

In this work,based on the high fluorescence quenching efficiency of polydopamine nanospheres(PDANS) and the specific recognition characteristic of aptamer,we construct a rapid,simple,sensitive and economical fluorescent biosensor for "turn-on" detection of ochratoxin A(OTA).In the absence of OTA,the fluorescence of FAM was readily quenched due to the strong binding between aptamer and *** the presence of OTA,the conformation of the OTA-specific aptamer changed from a random-coil to an antiparallel G-quadruplex,which resulted in the dissociation of the FAM from the surface of PDANS and the increasing of fluorescence *** method allowed quantitative detection of OTA with a detection limit of 20 ***,it was applied for the detection of OTA in red wine samples and showed satisfactory results.

关键词： polydopamine nanospheres Fluorescence Aptamer OTA detection Biosensor

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多关系关联规则挖掘中的隐私保护

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华中科技大学学报（自然科学版） 2007年第11期35卷 41-43页

作者：李专王元珍华中科技大学计算机科学与技术学院湖北武汉430074

分析了经典关联规则挖掘及相关的隐私保护等问题,同时研究了多关系关联规则的刻画和挖掘问题.通过重新定义查询模式,改进了Warmr方法,使查询模式支持"频繁查询模式的子模式也必然是频繁的"这种Apriori特性,进而将其移植到多关系规则的... 详细信息

分析了经典关联规则挖掘及相关的隐私保护等问题,同时研究了多关系关联规则的刻画和挖掘问题.通过重新定义查询模式,改进了Warmr方法,使查询模式支持"频繁查询模式的子模式也必然是频繁的"这种Apriori特性,进而将其移植到多关系规则的挖掘过程,从而加快规则的挖掘.研究了有针对性的敏感规则的挖掘方法,通过挖掘包含敏感信息的所有频繁查询模式,从中导出所有能够导致信息泄露的敏感规则;为了平衡数据可用性和安全性之间的矛盾,通过隐藏所有敏感规则中公共关系的元组,在保证规则隐藏和数据安全的同时,降低了对数据可用性的影响.

关键词：数据挖掘多关系关联规则频繁查询模式隐私保护

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