Exploring the Novel Genetic Variant of PITX1 Gene and Its Effect on Milk Performance in Dairy Goats

作     者：LAN Xian-yong ZHAO Hai-yu LI Zhuan-jian ZHOU Rui PAN Chuan-ying LEI Chu-zhao CHEN Hong 

作者机构：College of Animal Science and Technology Northwest A&F University/Shaanxi Key Laboratory of Molecular Biology for Agriculture College of Life Sciences Northwest A&F University 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2013年第12卷第1期

页      面：118-126页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：funded by the National Natural Science Foundation of China (31172184) the Young New Star Project on Science & Technology of Shaanxi Province, China(2011kjxx64) the Natural Science Foundation of Shaanxi Province of China (2011JQ3009) the Young Topnotch Researcher Support Project of Northwest A&F University,China (QNGG-2009-007) the Special Fund for Basic Scientific Research and Operation Expenses in Sci-Tech Innovation of Northwest A&F University, China(QN2011102) 

主　　题：dairy goat PITX1 gene genetic variant association milk performance 

摘      要：Paired-like homeodomain transcription factor 1 （PITX1） plays an important role in pituitary development by indirectly regulating the expression of the GH and PRL genes, and therefore PITX1 gene is regarded as a potential candidate gene for building the relationship between the gene polymorphism and milk traits. The aim of this study was to explore the novel genetic variant in PITX1 gene and its effect on milk performance in dairy goats. Herein, a novel genetic variation （NW_00314033： g.201GA or IVS1＋41GA） located at nt41 position of the first intron of the goat PITX1 gene was reported at the P1 locus, which can be genotyped by the Msp I PCR-RFLP. In the Msp I PCR-RFLP analyis, the GG variant was a major genotype, and the A variant was a minor allele in Guanzhong dairy goats which was at Hardy-Weinberg disequilibrium （chi-square χ2=140, P0.01）. The establishment of associations between different genotypes and milk performance was performed in the analyzed population. A total of three significant associations of the polymorphism with average milk fat content （%） （P=0.045）, morning milk fat content （%） （P=0.049）, and afternoon milk fat content （%） （P=0.050）, were found, respectively. A significant relationship between the polymorphism and average total solid content （P=0.029） was also detected. This novel single nucleotide polymorphism （SNP） extended the spectrum of genetic variation of the goat PITX1 gene, and its significant association with milk performance would benefit from the application of DNA markers related to improving milk performance through marker-assisted selection （MAS） in dairy goats.