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Synthesis of a new aromatic diacid containing pyridine ring and related polybenzimidazole

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Chinese Chemical Letters 2010年第8期21卷 976-978页

作者： Tao Ma Yan feng li shu Jiang Zhang feng Chun Yang Chen liang Gong Jiu Jiang Zhao College of Chemistry and Chemical Engineering State Key Laboratory of Applied Organic Chemistry Institute of Biochemical Engineering & Environmental TechnologyLanzhou UniversityLanzhou 730000China

A new aromatic diacid monomer containing pyridine unit,4-phenyl-2,6-bis[4-（4-carboxyphenoxy） phenyl]pyridine 3,was successfully synthesized in three steps,starting from *** corresponding pyridine-containing polybenzimidazole was prepared via polycondensation of 3 with tetraaminodiphenyl ether（TADE） in poly（phosphoric acid）（PPA）.The resulting polybenzimidazole exhibits excellent solubility,good thermal stabilities and high thermal resistance.

关键词： Polybenzimidazoles Pyridine-containing diacid monomer Synthesis Thermal stabilities

Determination of the gas compositions for direct coal liquefaction by gas chromatography

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Journal of Coal Science & Engineering(China) 2013年第4期19卷 535-539页

作者： Xiao-Man ZHU Pei-lin li shu-feng DU Beijing Research Institute of Coal Chemistry State Key Laboratory of Coal Mining and Clean Utilization National EnergyTechnology & Equipment Laboratory of Coal Utilization and Emission China Coal Research Institute Beijing 100013 China

Via multi-dimensional gas chromatography, configured with parallel dual-channel, double detectors, valves switching and back flushing, rapid analysis of the gas compositions consisting of C1-C5 hydrocarbons and permanent gases, such as CO2, H2S, H2, and CO, for direct coal liquefaction has been realized. With four packed chromatographic columns, which are Hayesep-Q pre-column, Hayesep-Q column, molecular sieve 5A column and one PLOT A1203 S capillary column, the gas compositions for direct coal liquefaction are analyzed qualitatively and quantitatively by the external standard method. The determination method has such advantages as excellent separation, simple operation, rapid analysis and accurate results.

关键词： gas chromatography gas compositions direct coal liquefaction external standard method

Pretreatment with interleukin-33 reduces warm hepatic ischemia/ reperfusion injury in mice

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Chinese Medical Journal 2013年第10期126卷 1855-1859页

作者： li shu ZHU feng-xue ZHANG Hong-bin li Hui AN You-zhong Department of Critical Care Medicine Peking University People'sHospital Beijing 100044 China

Background Interleukin （IL）-33 is a recently identified member of the IL-1 family that binds to the receptor, ST2L. This study examined IL-33 production in mouse liver and investigated its role in hepatic ischemia/reperfusion （I/R） injury. Methods Male BALB/c mice （（22＋3） g） were subjected to 90 minutes partial hepatic ischemia, followed by 6 hours reperfusion. First, mice were randomized into two groups： control group （laparotomy only, without blocking blood supply） and ischemia model group. IL-33 mRNA and serum protein levels were measured at 30, 60, 90 minutes after ischemia and 2 and 6 hours after reperfusion. Second, mice were randomized into four groups： control, model （injection of rabbit IgG polyclonal antibody）, recombinant IL-33 intervention and anti-ST2L antibody intervention group. Mice were sacrificed 6 hours after reperfusion. liver pathology was observed via transmission electron microscopy. Serum aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, IL-4, IL-5, IL-13, interferon-y （IFN-y） and tumor necrosis factor-a （TNF-a） levels were measured. Results Levels of IL-33 mRNA and protein did not change during ischemia （P 〉0.05） but increased significantly during reperfusion （P 〈0.05）. After reperfusion for 6 hours, serum levels of ALT, AST, IL-4, IL-5, IL-13, IFN-~ and TNF-a were significantly increased （P 〈0.05）, and hepatocellular ultrastructure was damaged. Pretreatment with IL-33 attenuated severity of liver damage compared with controls, but pretreatment with anti-ST2L antibody increased severity. Serum levels of IL-4, IL-5 and IL-13 protein increased whereas IFN-y decreased following IL-33 pretreatment. Pretreatment with anti-ST2L antibody significantly decreased serum IL-4, IL-5, IL-13 levels and increased serum IFN-r levels compared with controls （P 〈0.05）. There was no change in the level of TNF-a. Conclusion IL-33 is produced systematically and locally in liver during I/R injury. Pretreatment with IL-33 is therapeutic for hepatic

关键词： liver ischemia/reperfusion T helper lymphocyte,interleukin-33

On the Making of History and Other Problems

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Social Sciences in China 1985年第1期 51-63页

作者：黎澍 Historical Research and Social Sciences in China the Chinese Academy of Social Sciences

reexa- mines the formulation'the masses are the makers of history'and shows in terms of textual and logical analysis that this is a distortion of the ideas of both Marxand *** phrase actually originated in the Short History of the CPSU(B)published in the Soviet Union in the late *** was subsequently inter-preted as meaning that the production of material values constituted the wholeof historical development,In China the phrase was later amended into'themasses are the masters of history'and construed as implying that the mass ofthe people consistently played a leading role in the historical *** shushows that logically the former argument represents a reductionist view of his-tory which neglects the larger,multi-faceted historical process in favor of thepremise of the making of history,*** making of material *** similarfashion he demonstrates that the productive activities of the masses are the objectivebasis for historical development, but nonetheless the oppressed and exploited massescould often only play a passive *** historical role as'masters of history'began only in recent *** concludes with a presentation of Marx,Engels andLenin on the question of how'men make their own history.'He shows they spokein a more nuanced fashion and always with emphasis on the limitations of objectivecircumstances.

关键词： On the Making of History and Other Problems

Application of Zero Correlation Zone Sequences in Quasi-synchronous CDMA Systems

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Semiconductor Photonics and Technology 2007年第1期13卷 39-42,52页

作者： li shu-feng JIA Zhi-cheng WANG Jing li Qi School of Information Engineering Hebei University of Technology Tianjin 300130 CHN

In a quasi-synchronous code division multiple access(QS-CDMA) system, the correlation functions around the origin influence the system performance and odd functions are as important as the even functions. Three kinds of zero correlation zone(ZCZ) sequences are used to analyze and compare the correlation functions. Binary phase shift keying(BPSK) simulations of QS-CDMA system with the three kinds of QS sequences as spreading codes on additive white Gaussian noise(AWGN) channel show that these sequences are quasi-orthogonal, so they are suitable for QS-CDMA system. The correlation between the system performance and the zero correlation zone of the sequence is also proved.

关键词： QS-CDMA spreading sequence ZCZ sequence

Effect of MSTN Propeptide and shRNA Co-expression Vector on Proliferation of Skeletal Muscle Satellite Cells

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Journal of Northeast Agricultural University(English Edition) 2014年第1期21卷 31-38页

作者： feng lin-he Wang Xin Lu Ming Tong Hui-li li shu-feng Yan Yun-qin College of life Sciences Northeast Agricultural University

Myostatin （MSTN） is a negative regulator of skeletal muscle growth, in order to study the effect of inhibition MSTN expression on the proliferation of bovine skeletal muscle satellite cells, we constructed co-expression vector pcDNA3.1-Pro- MSTNshRNA, transfected it into muscle satellite cells by liposome 2000, and detected cell proliferation changes by CCK-8 method and flow cytometry after 48 h. The expressions of P21 and CDK2 were detected by Western blot and real-time PCR. The results showed that the cell vitality of experimental groups significantly increased than that of the negative control, and cells in S phase also increased significantly （P〈0.05）. After knocked down MSTN gene, P21 expression decreased （P〈0.05）, but CDK2 gene expression increased （P〈0.05）. These results indicated that MSTN gene expression was associated with P21 and CDK2, the proliferation of skeletal muscle satellite cells could be promoted while MSTN was inhibited, which provided a theoretical basis for the study on transgenic cattle.

关键词： myostatin cell proliferation flow cytometry expression vector

A Comparison Study on the Chinese and Western Music Aesthetics

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Comparative literature:East & West 2007年第1期 136-138页

作者： Ph.D.Candidate:li shu Supervisor:feng Xian’guang

Specialty:literary Theory Music Aesthetics is a basic and theoretical subject,which studies the basic rules of music,and especially regards the study of the particularity of music and aesthetics as its general *** to ... 详细信息

关键词： comparison between China and the West music aesthe

从“双师型”教师政策的演进看职业教育教师专业发展的定位

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教师教育研究 2014年第3期26卷 17-22页

作者：李树峰教育部职业技术教育中心研究所北京100029

"双师型"教师政策是国家在特定阶段解决职业教育技能型教师短缺问题而制定的方案,其宗旨在于优化教师队伍素质结构,提升职业教育质量。从"双师型"教师政策的演进过程来看,国家对于职业教育教师业务水平的要求越来越高,对于职业教育教师... 详细信息

"双师型"教师政策是国家在特定阶段解决职业教育技能型教师短缺问题而制定的方案,其宗旨在于优化教师队伍素质结构,提升职业教育质量。从"双师型"教师政策的演进过程来看,国家对于职业教育教师业务水平的要求越来越高,对于职业教育教师素质的要求越来越专业化。准确定位职业教育教师专业发展,对于加强"双师型"教师队伍建设十分必要。

关键词： “双师型”教师政策演进教师专业发展

Rapid Detection of Haemophilus influenzae and Haemophilus parainfluenzae in Nasopharyngeal Swabs by Multiplex PCR

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Biomedical and Environmental Sciences 2012年第3期25卷 367-371页

作者： TIAN Guo Zhong ZHANG li Juan WANG Xiao Lei ZHANG li li shu feng GU Chang Mei SUN Jian CUI Bu Yun State Key Laboratory for Infectious Diseases Control and Prevention National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Department of Physiology and Pathophysiology Basic Medical Sciences School of Capital Medical University Beo'ing 100069 China The Third People's Hospital of Chengdu City Chengdu 610031 Sichuan China Chengdu Children Hospital Chengdu 610017 Sichuan China Feicheng Mining Group Company Health and Epidemic Prevention Station Feicheng 2 71608 Shangdong China

Objective To establish multiplex PCR-based assays for detecting *** and ***. And the PCR-based assays were applied to detect the carriage rates of *** and *** in nasopharyngeal swab specimens which were collected from healthy children. Methods Multiplex primers for species-specific PCR were designed by using DNAstar soft based on the sequences of 165 rRNA genes from genus Haemophilus to detect *** and ***. Results The sensitivity of the 165 rRNA PCR assay for detecting *** and *** was 97.53% and 100% respectively, and the specificity was 95.89% and 96.63% respectively. Youden＇s Index on the ability to detect *** and *** was 0.9342 and 0.9663 respectively. 666 nasopharyngeal swab specimens were collected from healthy children. The detection rates of *** and *** were 14.11% and 16.07% respectively by using isolation and culture methods. The detection rates of *** and *** were 43.54% and 57.96% respectively by 165 rRNA PCR assays. The carriage rates of serotypes a, b, c, d, e, f and non-typeable isolates were 0% （0/666）, 0.15% （1/666）, 1.20% （8/666）, 0.15% （1/666）, 1.20% （8/666）, 1.80% （12/666）, 95.50% （636/666） respectively. Conclusion The multiplex PCR assays were very rapid, reliable and feasible methods for detection of *** and *** in pharyngeal swab specimens which were compared to conventional isolation and culture methods. 95.5% of *** strains in healthy children were nontypeable. The encapsulated or typable strains were mainly three serotypes which was c, e, and f serotype.

关键词： H.influenzae H.parainfluenzae Multiplex PCR