检索结果-南通市图书馆

N-methyl-D-aspartate receptor subunit 1 protein expression in the hippocampus and temporal cortex of kainic acid-induced epilepsy rats

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2010年第14期5卷 1045-1049页

作者： Zan Wang Hongyu jiang Suisheng Wu Hongmei Meng li ji li Cui Weihong lin Department of Neurology First Hospital of Jilin University Changchun 130021 Jilin Province China

BACKGROUND： The N-methyl-D-aspartate receptor subunit 1 （NMDAR1） contributes to the incidence of epilepsy. However, the relationship between epilepsy-induced brain injury and NMDAR1 remains poorly understood. OBJECTIVE： To investigate changes in NMDAR1 protein expression in the hippocampus and temporal cortex of kainic acid-induced epilepsy rats. DESIGN, TIME AND SETFING： A randomized, controlled, animal experiment was performed at the Department of Physiology and Department of Pathology, Basic Medical College of jilin University from March 2002 to March 2003. MATERIALS： Rabbit anti-NMDAR1 antibody was purchased from Wuhan Boster Biological Technology, China. METHODS： A total of 80 healthy, male, Wistar rats, aged 22 weeks, were randomly assigned to sham-surgery （n = 10） and model （n = 70） groups. Epilepsy models were established by injecting kainic acid （1μL） into the right amygdala, and rats were sacrificed at 2, 6, 24, 72 hours, and 7, 15, 30 days after surgery, with 10 animals at each time point. The rats in the sham-surgery group were injected with 1μL phosphate buffered saline into the right amygdala. MAIN OUTCOME MEASURES： NMDAR1 protein expression in the hippocampus and temporal cortex at 2, 6, 24, 72 hours and 7, 15, 30 days after epilepsy was detected using immunohistochemistry and flow cytometry analysis. RESULTS： In the sham-surgery group, a few NMDARl-positive cells were distributed in the hippocampus and temporal cortex. In the model group, NMDARl-positive cells were increased in the hippocampus and temporal cortex at 2 hours following kainic acid-induced epilepsy. They were significantly increased at 6 hours, and slightly decreased at 7 days （CA3 region and temporal cortex）, but remained greater than the sham-surgery group. This continued until day 30 （P 〈 0.01 ）. In addition, there were more NMDAR1 positive cells in the hippocampal CA3 and dentate gyrus than the temporal cortex （P 〈 0.01）. CONCLUSION： In epilepsy model rats, NMDAR1 protein ex

关键词： kainic acid epilepsy N-methyI-D-aspartate receptor rat subunit neural regeneration

Role of PGC-1αin muscle function and aging

在线全文

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2013年第2期2卷 81-86页

作者： Chounghun Kang li li ji Laboratory of Physiological Hygiene and Exercise Science School of KinesiologyUniversity of Minnesota

This article focuses on the current underlying of molecular mechanisms of the peroxisome proliferator-activated receptor-γ coactivator-1α （PGC-1α） mediated pathway and discuss possible therapeutic benefits of increased mitochondrial biogenesis in compensating for mitochondrial dysfunction and ameliorating aging and aging-related diseases. PGC-1α is the master transcription regulator that stimulates mitochondrial biogenesis, by upregulating nuclear respiratory factors and mitochondrial transcription factor A, leading to increased mitochondrial DNA replication and gene transcription. PGC-1α also regulates cellular oxidant-antioxidant homeostasis by stimulating the gene expression of superoxide dismutase-2, catalase, glutathione peroxidase 1, and uncoupling protein. Recent reports from muscle-specific PGC-1α overexpression underline the benefit of PGC-1α in muscle atrophy and sarcopenia, during which PGC-1α enhanced mitochondrial biogenic pathway and reduced oxidative damage. Thus, PGC-1α seems to have a protective role against aging associated skeletal muscle deterioration.

关键词： Aging Mitochondria Muscle PGC-1α Signaling

Healthy aging:Cellular insights

在线全文

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2013年第2期2卷 65-66页

作者： li li ji School of Kinesiology Laboratory of Physiological Hygiene and Exercise ScienceUniversity of Minnesota111 Cooke Hall1900 University Avenue

Aging is a multifactorial process characterized by deteriorations of functional capacity of various organs and the whole body performance. Exogenous mechanism for aging includes onset and progress of various degenerative diseases, whereas intrinsic mechanism is governed by cellular events wherein catabolic processes exceed the capacity for repair and rejuvenation.

关键词：细胞老龄化健康老化机制退行性疾病器官功能代谢过程

Reactive oxygen species promote endurance exercise-induced adaptations in skeletal muscles

在线全文

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2024年第6期13卷 780-792页

作者： Scott K.Powers Zsolt Radak li li ji Malcolm Jackson Department of Applied Physiology University of FloridaGainesvilleFL 32608USA Research Institute of Sport Science Hungarian University of Sport ScienceBudapest 1123Hungary Department of Kinesiology University of MinnesotaSt.PaulMN 55455USA Department of Musculoskeletal and Ageing Science Institute of Life Course and Medical SciencesUniversity of LiverpoolLiverpool L78TXUK

The discovery that contracting skeletal muscle generates reactive oxygen species(ROS)was first reported over 40 years *** prevailing view in the 1980s was that exercise-induced ROS production promotes oxidation of proteins and lipids resulting in muscle ***,a paradigm shift occurred in the 1990s as growing research revealed that ROS are signaling molecules,capable of activating transcriptional activators/coactivators and promoting exercise-induced muscle *** evidence supports the notion that reduction-oxidation(redox)signaling pathways play an important role in the muscle remodeling that occurs in response to endurance exercise *** review examines the specific role that redox signaling plays in this endurance exercise-induced skeletal muscle *** begin with a discussion of the primary sites of ROS production in contracting muscle fibers followed by a summary of the antioxidant enzymes involved in the regulation of ROS levels in the *** then discuss which redox-sensitive signaling pathways promote endurance exercise-induced muscle adaptation and debate the strength of the evidence supporting the notion that redox signaling plays an essential role in muscle adaptation to endurance exercise *** hopes of stimulating future research,we highlight several important unanswered questions in this field.

关键词： Antioxidants Mitochondrial biogenesis Radicals Redox signaling

Design of Track/Hold (T/H) Circuit of Broad Bandwidth and High Speed Pipeline Structure ADC Based on the Super Frequency Application

在线全文

学校读者我要写书评

暂无评论

Journal of Electronic Science and Technology of China 2007年第4期5卷 358-361页

作者： ji-An Wang Wei li Zhao-ji li School of Microelectronics and Solid-State Electronic University of Electronic Science and Technology ofChina （UESTC） Chengdu 610054 China

A track/hold （T/H） circuit of broad bandwidth high speed pipeline structure ADC based on the super frequency application is designed in the paper. Some main factors affecting SNR of high speed ADC, such as aperture uncertainty, switch capacitor, and MOS switch, are analyzed. In the circuit, the full-differential structure and the bottom plate sampling technique are adopted to optimize the switch capacitors and MOS switches. The result based on the Spectre simulation on 0.35pm Bi- CMOS technology indicate that the aperture uncertainty, charge-injection, and non-linearity of clock feed-through are considerably restrained and the performance of T/H circuit is enhanced

关键词： ADC aperture uncertainty MOS switch switch capacitance track and hold.

Mitochondrial redox metabolism in aging:Effect of exercise interventions

在线全文

学校读者我要写书评

暂无评论

Journal of Sport and Health Science 2013年第2期2卷 67-74页

作者： Hai Bo Ning jiang li li ji Yong Zhang Tianjin Key Laboratory of Exercise Physiology and Sports Medicine Department of Health & Exercise ScienceTianjin University of Sport Department of Military Training Medicines Logistics University of Chinese People's Armed Police Force Laboratory of Physiological Hygiene and Exercise Science School of KinesiologyUniversity of Minnesota

Mitochondrial redox metabolism has long been recognized as being central to the effects of aging and the development of age-related pathologies in the major oxidative organs. Consistent evidence has shown that exercise is able to retard the onset and impede the progression of aging by modifying mitochondrial oxidant--antioxidant homeostasis. Here we provide a broad overview of the research evidence showing the relationship between mitochondrial redox metabolism, aging and exercise. We address part aspects of mitochondrial reactive oxygen species （ROS） metabolism, from superoxide production to ROS detoxification, especially antioxidant enzymes and uncoupling protein. Furthermore, we describe mitochondrial remodeling response to aging and exercise, which is accompanied by bioenergetics and redox regulation. In addition, potential mechanisms for redox signaling involved in mitochondrial remodeling and redox metabolism regulation are also reviewed.

关键词： Aging Exercise Mitochondrial remodeling PGC-1α Reactive oxygen species

Clinical and electrophysiological profiles in early recognition of polyneuropathy, organomegaly, endocrinopathy, M-protein, and skin changes syndrome

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2019年第14期132卷 1666-1672页

作者： Qin Wang Peng liu li-li ji Shuai Wu Guo-Dong Feng Xin Wang ji-Hong Dong Department of Neurology Zhongshan Hospital Fudan UniversityShanghai 200032China Department of Haematology Zhongshan Hospital Fudan UniversityShanghai 200032China

Background:The detection of polyneuropathy,organomegaly,endocrinopathy,M-protein,and skin changes (POEMS) syndrome at early stage is challenging for *** polyneuropathy could be the first manifestation,it could be misdiagnosed as chronic inflammatory demyelinating polyneuropathy (CIDP).The present study aimed to determine the clinical and electrophysiological features of POEMS syndrome to distinguish from ***:The data of a group of patients with POEMS (n =17) and patients with CIDP (n =17) in Zhongshan Hospital Fudan University from January 2015 to September 2017 were analyzed in this retrospective *** clinical features,neurological symptoms,and electrophysiological findings were compared between the two ***:Clinically,patients with POEMS demonstrated significantly more neuropathic pain in the lower extremities than patients with CIDP (58.8 % vs.11.8 %,P =0.01).Multisystem features like edema,skin change,organomegaly,and thrombocytosis were also pointed towards the diagnosis of POEMS ***,terminal latency index (Tli) was significantly higher in patients with POEMS than that in patients with CIDP (median nerve:0.39 [0.17-0.52] vs.0.30 (0.07-0.69),Z =-2.413,P =0.016;ulnar nerve:0.55 [0.23-0.78] vs.0.42 [0.12-0.70],Z =-2.034,P =0.042).Patients with POEMS demonstrated a higher frequency of absent compound muscle action potential of the tibial nerve (52.9% vs.17.6%,P =0.031),less conduction block (ulnar nerve:0 vs.35.3%,P =0.018),and less temporal dispersion (median nerve:17.6% vs.58.8%,P =0.032) than CIDP *** combination of positive serum monoclonal protein and high Tli (if either one or both were present) discriminated POEMS from CIDP with a sensitivity of 94.1% and 47.1% and specificity of 76.5% and 100.0%,***:POEMS syndrome could be distinguished from CIDP through typical clinical and electrophysiological characteristics in *** combination of serum monoclonal protei

关键词： Polyneuropathy Organomegaly Endocrinopathy M protein and skin changes syndrome Chronic inflammatory demyelinating polyradiculoneuropathy Terminal latency index

Chlorogenic acid prevents acetaminophen-induced liver injury: the involvement of CYP450 metabolic enzymes and some antioxidant signals

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第7期16卷 602-610页

作者： Chun PANG Yu-chen SHENG Ping jiANG Hai WEI li-li ji Shanghai Key Laboratory of Complex Prescription the MOE Key Laboratory for Standardization of Chinese MedicinesInstitute of Chinese Materia Medica Shanghai University of Traditional Chinese Medicine Center for Traditional Chinese Medicine and Systems Biology Shanghai University of Traditional Chinese Medicine Center for Drug Safety Evaluation and Research Shanghai University of Traditional Chinese Medicine

Chlorogenic acid（CGA）, a polyphenolic compound, is abundant in fruits, dietary vegetables, and some medicinal herbs. This study investigated the prevention of CGA against acetaminophen（AP）-induced hepatotoxicity and its engaged mechanisms. CGA reversed the decreased cell viability induced by AP in L-02 cells in vitro. In addition, CGA reduced the AP-induced increased serum levels of alanine/aspartate aminotransferase（ALT/AST） in vivo. The effect of CGA on cytochrome P450（CYP） enzymatic（CYP2E1, CYP1A2, and CYP3A4） activities showed that CGA caused very little inhibition on CYP2E1 and CYP1A2 enzymatic activities, but not CYP3A4. The measurement of liver malondialdehyde（MDA）, reactive oxygen species（ROS）, and glutathione（GSH） levels showed that CGA prevented AP-induced liver oxidative stress injury. Further, CGA increased the AP-induced decreased m RNA expression of peroxiredoxin（Prx） 1, 2, 3, 5, 6, epoxide hydrolase（Ephx） 2, and polymerase（RNA） II（DNA directed） polypeptide K（Polr2k）, and nuclear factor erythroid-2-related factor 2（Nrf2）. In summary, CGA ameliorates the AP-induced liver injury probably by slightly inhibiting CYP2E1 and CYP1A2 enzymatic properties. In addition, cellular important antioxidant signals such as Prx1, 2, 3, 5, 6, Ephx2, Polr2 k, and Nrf2 also contributed to the protection of CGA against AP-induced oxidative stress injury.

关键词： Chlorogenic acid Acetaminophen CYP450 Oxidative stress injury

STUDIES ON THE SEGMENTAL STRUCTURE OF POLYESTER-POLYETHER

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Polymer Science 1989年第2期7卷 143-149页

作者：陈传福王身国李学芬李执芬冀克俭李立璞 Institute of Chemistry Academia SinicaBeijing

In this paper the change of segment ratio, segmental sequence distribution and randonmess B of PET-PTMG block copolymer have been investigated by IR and ^(13)C-NMR spectra during the polycondensation reaction.

关键词： Polyester-polyether block copolymer Segmental structure Sequence distribution Probability Randomness.