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A New Half-life Value of ^(79)Se

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Chinese Physics Letters 1996年第1期13卷 9-12页

作者： HANG Song-sheng GUO Jing-ru JIANG Shan li chun-sheng CUI An-zhi HE Ming China Institute of Atomic Energy Beijing 102413

In the recent charts of the nuclides an accepted half-life of ^(79)Se is only an upper limit,<6.5×10^(4)*** this letter,A new half-life of ^(79)Se has been measured tobe(1.13±0.17)×10^(6)*** number of ^(79)Se atoms... 详细信息

In the recent charts of the nuclides an accepted half-life of ^(79)Se is only an upper limit,life of ^(79)Se has been measured tobe(1.13±0.17)×10^(6)*** number of ^(79)Se atoms was determined from measured ^(79)Se/Se ratios with the accelerator mass spectrometry technique and the decay rate of ^(79)Se was determined by counting the emitted β rays with a liquid scintillationspectrorneter.

关键词： letter decay limit

Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第2期9卷 121-131页

作者： li-jiang WANG chun-sheng WU Zhao-ying HU Yuan-fan ZHANG Rong li Ping WANG Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China

By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid (MHDA) was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide (NHS) raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor’s resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0×102 colony forming units (CFU)/ml E. coli O157:H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly (LBL-SA) method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed.

关键词： Biosensor Escherichia coli O157:H7 Immunosensor Layer-by-layer self-assembly (LBL-SA) Quartz crystalmicrobalance (QCM)

Ulinastatin Exerts the Protective Effects of Lung by UpRegulating Aquaporins Expression in a Two-Hit Porcine Model of Acute Respiratory Distress Syndrome

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Biomedical and Environmental Sciences 2021年第12期34卷 1029-1032页

作者： HANG Chen Chen GUO Yu Hong li chun sheng WANG Shuo Emergency medicine clinical research center of Beijing Chao-Yang Hospital Capital Medical UniversityBeijing Key Laboratory of Cardiopulmonary Cerebral ResuscitationBeijing 100020China Medical Affairs Office Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing 100010China Department of emergency medicine Beijing Friendship HospitalCapital Medical UniversityBeijing 100050China Department of infectious diseases(Fever clinic) Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing 100010China

Acute respiratory distress syndrome(ARDS)is a syndrome characterized by significant lung edema,impaired permeability of lung microvascular endothelial cells with the mal-expression of aquaporins(AQPs).In this study,a two-hit porcine model of ARDS induced by smoking and methicillinresistant Staphylococcus aureus(MRSA)was developed,we investigate the AQPs expression and the influence of Ulinastatin(UTI)to ***-nine healthy male domestic pigs aged 8–10 weeks and weighing 30±2 kg were used for the *** was maintained through continuous intravenous infusion of pentobarbital[8 mg/(kg∙h)].A 5-Fr PiCCO catheter(Pulsiocath PV2015L20;Pulsion Medical Systems,Munich,Germany)and a central venous catheter was inserted for hemodynamic monitoring,including mean arterial pressure(MAP),cardiac index(CI),systemic vascular resistance index(SVRI)and extravascular lung water index(ELWI).

关键词： Acute lung impaired

Epinephrine in Out-of-hospital Cardiac Arrest： Helpful or Harmful？

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Chinese Medical Journal 2017年第17期130卷 2112-2116页

作者： Huan Shao chun-sheng li Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China

Objective：Epinephrine is the primary drug administered during cardiopulmonary resuscitation （CPR） to reverse cardiac *** evidence for the use of adrenaline in out-of-hospital cardiac arrest （OHCA） and in-hospital resuscitation is *** conducted a systematic review on the clinical efficacy of adrenaline in adult OHCA patients to evaluate whether epinephrine provides any overall benefit for *** Sources：The EMBASE and PubMed databases were searched with the key words ＆quot;epinephrine,＆quot; ＆quot;cardiac arrest,＆quot; and variations of these *** Selection：Data from clinical randomized trials,meta-analyses,guidelines,and recent reviews were selected for ***：Sudden cardiac arrest causes 544,000 deaths in China each year,with survival occurring in 〈1% of cases （compared with 12% in the United States）.The American Heart Association recommends the use of epinephrine in patients with cardiac arrest,as part of advanced cardiac life *** is a clear evidence of an association between epinephrine and increased return of spontaneous circulation （ROSC）.However,there are conflicting results regarding long-term survival and functional recovery,particularly neurological outcome,after *** is currently insufficient evidence to support or reject epinephrine administration during *** believe that epinephrine may have a role in resuscitation,as administration of epinephrine during CPR increases the probability of restoring cardiac activity with pulses,which is an essential intermediate step toward long-term ***：The administration of adrenaline was associated with improved short-term survival （ROSC）.However,it appears that the use of adrenaline is associated with no benefit on survival to hospital discharge or survival with favorable neurological outcome after OHCA,and it may have a harmful *** placebo-controlled,double-blind,randomized control trials are required to definitively establis

关键词： Adrenaline Cardiac Arrest Cardiopulmonary Resuscitation: Epinephrine Return of Spontaneous Circulation

Programmed Cell Death-I/Programmed Death-ligand 1 Pathway： A New Target for Sepsis

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Chinese Medical Journal 2017年第8期130卷 986-992页

作者： Qiang liu chun-sheng li Intensive Care Unit Central Hospital of Dandong City Dandong Liaoning 118002 China Department of Emergency Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation Beijing 100020 China

Objective： Sepsis remains a leading cause of death in many Intensive Care Units worldwide, lmmunosuppression has been a primary locus of sepsis research as a key pathophysiological mechanism. Given the important role of the negative costimulatory molecules programmed cell death-1 （PD-1） and programmed death-ligand 1 （PD-li） in the occurrence of immunosuppression during sepsis, we reviewed literatures related to the PD-1/PD-L 1 pathway to examine its potential as a new target for sepsis treatment. Data Sources： Studies of the association between PD-I/PD-li and sepsis published tip to January 31, 2017, were obtained by searching tile PubMed database. Study Selection： English language studies, including those based on animal models, clinical research, and reviews, with data related to PD- 1/PD-L I and sepsis, were evaluated. Results： lmmunomodulatory therapeutics could reverse the deactivation of immune cells caused by sepsis and restore immune cell activation and function. Blockade of＇the PD-1/PD-li pathway could reduce the exhaustion ofT-cells and enhance the proliferation and activation ofT-cells. Conclusions： The anti-PD- I/PD-L 1 pathway shows promise as a new target for sepsis treatment. This review provides a basis for clinical trials and Iiiture studies aimed at revaluating the efficacy and safety of this targeted approach.

关键词： lmmunosuoDression Programmed Cell Death-l Programmed Death-ligand 1 Sepsis

Protective Effect of Shenfu Injection on Vascular Endothelial Damage in a Porcine Model of Hemorrhagic Shock

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Chinese Journal of Integrative Medicine 2022年第9期28卷 794-801页

作者： ZHANG Ming-qing ZHANG Qiang YUAN Wei WU Jun-yuan liANG Yong QIN Hong-jie li chun-sheng Department of Emergency Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing100050China Department of Critical Care Medicine Peking University Third HospitalBeijing100191China Department of Emergency Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing100020China Department of Emergency Medicine Beijing Luhe HospitalCapital Medical UniversityBeijing101199China

Objective:To investigate the effects of Shenfu Injection(SFI)on endothelial damage in a porcine model of hemorrhagic shock(HS).Methods:After being bled to a mean arterial pressure of40±3 mm Hg and held for 60 min,32 pigs were treated with a venous injection of either shed blood(transfusion group),shed blood and saline(saline group),shed blood and SFI(SEI group)or without resuscitation(sham group).Venous blood samples were collected and analyzed at baseline and 0,1,2,4,and 6 h after *** necrosis factor-α(TNF-α),serum interleuking(IL)-6,and IL-10 levels were measured by enzymelinked immunosorbent assay(EliSA);expressions of vascular cell adhesion molecule-1(VCAM-1),intercellular adhesion molecule 1(ICAM-1),von Willebrand factor(vWF),plasminogen activator inhibitor-1(PAI-1)and Bcl-2,Bax,and caspase-3 proteins were determined by Western ***:The serum level of TNF-αin the SFI group was significantly lower than in the other groups at 0,1,and 2 h after HS,while the level of IL-6 was lower at 4 and 6 h compared with the saline group(Plial injury in the porcine model of HS by inhibiting cell apoptosis,suppressing the formation of proinflammatory cytokines,and reducing endothelial activation.

关键词： hemorrhagic shock Shenfu Injection vascular endothelial damage Chinese medicine

Effect of Splenic Regulatory T-cell Apoptosis on the Postresuscitation Immune Dysfunction in a Porcine Model

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Chinese Medical Journal 2016年第13期129卷 1577-1583页

作者： Wei Gu Qian Zhang chun-sheng li Department of Emergency Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation Beijing 100020 China

Background： Postresuscitation immune dysfunction contributes to the low survival rate after successful resuscitation, but its mechanism remains poorly understood. The purpose of this study was to investigate whether splenic regulatory T-cell （Treg） apoptosis was involved in the postresuscitation immune dysfunction. Methods： Thirty-eight pigs were randomly divided into sham-operated group （SHAM group, n - 8）, 12 h post return of spontaneous circulation （ROSC） group, 24 h post-ROSC group, and 48 h post-ROSC group （n = 10 per group）. A Wuzhishan miniature porcine model of 8-min ventricular fibrillation cardiac arrest （CA） was established. The apoptosis rates of Treg in the spleen were tested by flow cytometry; the expressions of forkhead/winged helix transcription factor （Foxp3） of Treg in the spleen were detected by real-time polymerase chain reaction; and the levels ofinterleukin-4 （IL-4）, IL- 10, and interferon gamma （IFN-γ） of Treg in the spleen were detected by enzyme-linked immunosorbent assay. Results： The apoptosis rates of Treg in all post-ROSC groups were significantly lower than that of SHAM group （7.7% ± 1.9%, 7.1% ±1 .8%, 6.2% ± 0.4% vs. 13.1% ± 1.6%： P 〈 0.05）; the expression levels of Foxp3 and IL- 10 were also decreased with the increase ofapoptosis rates of Treg. Helper T-cells CD4^＋ lymphocyte subsets were significantly lower in the post-ROSC groups compared with SHAM group （29. 1% ± 2.2%, 24.3% ± 2.2%, 24.1% ± 2.5% vs. 43.8%± 4.5%; P 〈 0.01 ） at 12, 24, and 48 h after ROSC. Compared with SHAM group, the levels of IFN-,/（161.0 ± 12.9, 167.7 ± 10.5, 191.2 ± 7.7 vs. 7.6 ± 0.9 rig/L） and 1L-4 （27.7 ± 6.2, 35.9 ± 3.5, 50.6 ± 6.1 vs. 13.3 i 2.3 ng/L） and the ratio of IFN-y/IL-4 （8.6 ± 2.3, 4.9 ± 0.4, 4.5 ± 0.9 vs. 0.8 ± 0.2） were all greatly elevated in all post-ROSC groups （P 〈 0.05）. Conclusions： Apoptosis rate of Treg was significantly decreased after CA, and thus the proportion of Treg was increased and the inhibitory effects were enhanced, which furth

关键词： Apoptosis Cardiac Arrest Foxp3: Immunity

Hypothermia ameliorates gastrointestinal ischemic injury sustained in a porcine cardiac arrest model

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Chinese Medical Journal 2012年第24期125卷 4406-4411页

作者： LU Yi WANG Shuo li chun-sheng Department of Emergency Medicine Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China

Background During cardiac arrest, the gastrointestinal tract is sensitive to ischemia. Protection of the gastrointestinal tract is a critical factor in determining prognosis following cardiopulmonary resuscitation （CPR）. This study seeks to determine the extent of gastrointestinal tract injury and the potential protective effect of inducing hypothermia following a porcine cardiac arrest model and CPR. Methods Ventricular fibrillation was induced by programmed electrical stimulation in 16 male domestic pigs （n=8 per group）. Four minutes after ventricular fibrillation, CPR was performed. Pigs that successfully restored spontaneous circulation then received intravenous infusions of saline at either 4℃ or room temperature to produce hypothermic and control conditions respectively. Serum diamine oxidase and gastrointestinal adenosine triphosphate enzyme activity were determined and histopathology of the gastrointestinal tract was performed by light microscopy and electron microscopy. Results Significant injury of the gastrointestinal tract after CPR was found. Na＋-K＋ and Ca2＋ adenosine triphosphate enzyme activity in the gastric tissue were significantly high in animals receiving hypothermia treatment compared to controls. Hypothermia also significantly reduced serum diamine oxidase after CPR compared to the control group. Moreover, severe injury sustained by the gastrointestinal tissue was significantly ameliorated under hypothermic conditions compared to controls. Conclusions Gastrointestinal injury and abnormal energy metabolism are strikingly evident following CPR. Hypothermia, which is induced by an infusion of 4℃ saline, can rapidly reduce internal body temperature, improve energy metabolism, and ameliorate injury to the gastrointestinal mucosa after CPR.

关键词： cardiac arrest cardiopulmonary resuscitation gastrointestinal tract hypothermia ischemic injury

Effects of Shenfu Injection(参附注射液)on Inflammatory Response during Post-Resuscitation Myocardial Dysfunction after Cardiac Arrest in Swine

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Chinese Journal of Integrative Medicine 2021年第6期27卷 417-423页

作者： GU Wei HOU Xiao-min li chun-sheng Department of Emergency Medicine Beijing Friendship HospitalCapital Medical UniversityBeijing Key Laboratory of Cardiopulmonary Cerebral ResuscitationBeijing(100050)China Department of Emergency Medicine Beijing Chaoyang HospitalCapital Medical UniversityBeijing Key Laboratory of Cardiopulmonary Cerebral ResuscitationBeijing(100020)China Now the author is at Emergency Medicine Center Chui Yang Liu Hospital Affiliated to Tsinghua UniversityBeijing(100022)China

Objective:To investigate whether Shenfu Injection(SFI,参附注射液)can alleviate post-resuscitation myocardial dysfunction by inhibiting the inflammatory ***:After 8 min of ventricular fibrillation and 2 min of basic life support,24 pigs were randomly divided into 3 groups(n=8),which were given intravenous bolus injections of SFI(1.0 mL/kg),epinephrine(EP,0.02 mg/kg)and normal saline(SA),*** animals were sacrificed at 24 h after restoration of spontaneous circulation(ROSC),and serum interleuking-6(IL-6)and tumor necrosis factor-α(TNF-α)levels were measured by enzyme-linked immunosorbent assay(EliSA);expressions of Toll-like receptor 4(TLR4)/nuclear factor kappa B(NF-κB)m RNAs and proteins were determined by RT-PCR and Western blot,***:Compared with the EP and the SA groups,the ultrastructure of myocardial cells were slightly damaged and the systolic function of the left ventricle was markedly improved in the SFI group at 24 h after ROSC(Pling pathway may be involved in the pathological mechanisms of post-resuscitation myocardial *** may block NF-κB-mediated inflammatory response by reducing the activity of NF-κB and the level of TNF-α,thus playing a protective role in post-resuscitation myocardial dysfunction.

关键词： Shenfu Injection Chinese medicine cardiopulmonary resuscitation post-resuscitation myocardial dysfunction TLR4/NF-κB