Sensing Escherichia coli O157:H7 via frequency shift through a self-assembled monolayer based QCM immunosensor

作     者：Li-jiang WANG Chun-sheng WU Zhao-ying HU Yuan-fan ZHANG Rong LI Ping WANG 

作者机构：Biosensor National Special Laboratory Key Laboratory of Biomedical Engineering of Ministry of Education Department of Biomedical Engineering Zhejiang University Hangzhou 310027 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2008年第9卷第2期

页      面：121-131页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 08[工学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Project supported by the Talent Foundation of Zhejiang Province (No. R205502) the Program of Education Department of Zhejiang Province (No. 20040197), China 

主　　题：Biosensor Escherichia coli O157:H7 Immunosensor Layer-by-layer self-assembly (LBL-SA) Quartz crystalmicrobalance (QCM) 

摘      要：By means of the specific immuno-recognition and ultra-sensitive mass detection, a quartz crystal microbalance (QCM) biosensor for Escherichia coli O157:H7 detection was developed in this work. As a suitable surfactant, 16-mercaptohexadecanoic acid (MHDA) was introduced onto the Au surface of QCM, and then self-assembled with N-hydroxysuccinimide (NHS) raster as a reactive intermediate to provide an active interface for the specific antibody immobilization. The binding of target bacteria with the immobilized antibodies decreased the sensor’s resonant frequency, and the frequency shift was correlated to the bacterial concentration. The stepwise assembly of the immunosensor was characterized by means of the electrochemical techniques. Using the immersion-dry-immersion procedure, this QCM biosensor could detect 2.0×102 colony forming units (CFU)/ml E. coli O157:H7. In order to reduce the fabrication time, a polyelectrolyte layer-by-layer self-assembly (LBL-SA) method was adopted for fast construction. Finally, the reproducibility of this biosensor was discussed.