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黑龙江教师发展学院学报 2024年第7期43卷 140-143页

作者：丘丽红厦门南洋职业学院教育学院福建厦门361000

民间游戏经过长期的发展与沉淀,积累了丰富的地域文化,颇具民族、地方特色,是弘扬中华优秀文化的重要载体。幼儿园课程设计直接影响幼儿成长,游戏是幼儿园教学中重要的方式,游戏具有独特的价值,将民间游戏融入幼儿园课程是一举多得。

关键词：民间游戏幼儿园课程融入的价值实施建议

Model parameters estimation of aero-engine based on hybrid optimization algorithm

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航空动力学报 2011年第7期26卷 1665-1671页

作者： li qiu-hong li Ye-bo JIANG Dian-wen (College of Energy and Power EngineeringNanjing University of Aeronautics and Astronautics Nanjing 210016 China

A hybrid optimization algorithm for the time-domain identification of multivariable,state space model for aero-engine was presented in this *** optimization procedure runs particle swarm optimization(PSO) and least squares optimization(LSO) "in series".PSO starts from an initial population and searches for the optimum solution by updating ***,it can sometimes run into a suboptimal *** LSO can start from the suboptimal solution of PSO,and get an optimum solution by conjugate gradient *** algorithm is suitable for the high-order multivariable system which has many parameters to be estimated in wide *** optimization algorithm is applied to estimate the parameters of a 4-input 4-output state variable model(SVM) for *** simulation results demonstrate the effectiveness of the proposed algorithm.

关键词： aero-engine state variable model(SVM) particle swarm optimization(PSO) least squares optimization(LSO) hybrid optimization algorithm

Identification of the 2-tridecanone cis-acting element in the promoter of cytochrome P450 CYP6B7 in Helicoverpa armigera

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Insect Science 2018年第6期25卷 959-968页

作者： li Xu Dong-Zhi li Yuan-Yuan Luo Jian-Ying Qin li-hong qiu College of Science China Agricultural UniversityBeijingChina

The expression level of cytochrome P450 genes in insects can be induced by plant allelochemicals,which is important for insects to adapt to host *** P450CYP6B 7has been reported to be involved in pyethroid insecticide resistance in Heli- coverpa armigera,and its transcription level was induced by some ***,the regulatory mechanism of the induced expression of CYP6B7remains unknown,although it is very important for understanding the detoxification mechanism to allelochemicals in host *** objective of the present study was to investigate the eis-acting ele- ment in the promoter of CYP6B7 mediating the inducible up-regulation of CYP6B7in *** by *** promoter region of CYP6B7was cloned by genome walking technique and analyzed by transient transfeetion *** 5'deletion of the promoter region of CYP6B7revealed that the relative luciferase activity of construct -320/+232could be significantly induced by *** stepwise deletion between -320 and -238 bp found that construct -292/+232 could also be significantly induced by 2-tridecanone,but the adjacent construct -256/+232could not,suggesting the essential role of the sequence between -292 and --257 bp for 2-tridecanone induction. Nucleotide mutations between -292 and -281 bp had no influence on the induction ef- fect by 2-tridecanone,but nucleotide mutations between -280 and -257 bp significantly decreased the induction *** results demonstrated that the cis-acting element for 2-trideeanone induction was between -280 and -257 bp in the promoter of CYP6B7.

关键词： cis-acting element cytochrome P450 CYP6B7 Helicoverpa armigera induction 2-tridecanone

神经纤毛蛋白-1在前成骨细胞成骨分化过程中及小鼠胫骨组织中的表达研究

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中国实用口腔科杂志 2024年第5期17卷 568-573页

作者：姚澳康李宗谕杨心月杨谛仇丽鸿于雅琼中国医科大学附属口腔医院牙体牙髓病科辽宁省口腔疾病重点实验室辽宁沈阳110002

目的探究神经纤毛蛋白-1(neuropilin 1,NRP1)在前成骨细胞MC3T3-E1成骨分化过程中的m RNA和蛋白表达特征,以及其在小鼠胫骨组织中的表达情况,为临床应用信号素3A/NRP1信号轴治疗慢性根尖周炎区骨吸收提供实验基础。方法研究于2023年4—... 详细信息

目的探究神经纤毛蛋白-1(neuropilin 1,NRP1)在前成骨细胞MC3T3-E1成骨分化过程中的m RNA和蛋白表达特征,以及其在小鼠胫骨组织中的表达情况,为临床应用信号素3A/NRP1信号轴治疗慢性根尖周炎区骨吸收提供实验基础。方法研究于2023年4—6月在中国医科大学附属口腔医院中心实验室进行。成骨分化诱导培养MC3T3-E1,并根据成骨分化时间分组。采用Western blot检测对照组(0 d)和分化1、3、5、7、10、14、20 d组的NRP1蛋白表达情况;采用qRT-PCR检测对照组和分化1、3、5、7、10、14 d组的NRP1 mRNA表达情况;对照组和成骨分化3、5 d组细胞进行免疫荧光染色,采用激光共聚焦显微镜观察成骨分化前期NRP1亚细胞定位。将3只4日龄小鼠共6根胫骨均分为NRP1组和阴性对照IgG组,并进行免疫荧光染色,采用倒置荧光显微镜观察NRP1在小鼠胫骨组织中的表达情况。结果各组NRP1蛋白及mRNA的相对表达量比较,差异均有统计学意义(F值分别为48600.000、60.030,均P<0.001);且分化7、10 d组的NRP1蛋白相对表达量较其他时间组高,分化10 d组的NRP1 mRNA相对表达量最高,差异均有统计学意义(均P<0.05)。在Western blot检测中NRP1主条带下方观察到分泌型NRP1(soluble NRP1,sNRP1)副条带。对照组和分化3、5 d组中,NRP1均在细胞质中表达,其亚细胞定位表现为围绕细胞核周围。NRP1在小鼠胫骨干骺端表达,且成骨区成骨细胞内表达广泛。结论NRP1参与前成骨细胞成骨分化过程,成骨分化前期主要在细胞质中表达,且参与小鼠胫骨形成。

关键词：神经纤毛蛋白-1 前成骨细胞成骨分化

2011—2022年北京市通州区重要人体寄生虫病监测结果及居民认知行为调查

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寄生虫与医学昆虫学报 2024年第2期31卷 78-84页

作者：陈立新李秋虹艾薇朴锦龙白连军张杰北京市通州区疾病预防控制中心北京101100 潞城社区卫生服务中心北京101117 台湖社区卫生服务中心北京101111 马驹桥社区卫生服务中心北京101102 牛堡屯社区卫生服务中心北京101104

目的分析北京市通州区2011—2022年寄生虫病监测和调查结果,为制订防控措施提供科学依据。方法从疾病信息报告管理系统和流行病学调查资料分析寄生虫病特征。按照北京市人体重点寄生虫病现状调查方案,采用分层整群抽样方法抽取调查点,... 详细信息

目的分析北京市通州区2011—2022年寄生虫病监测和调查结果,为制订防控措施提供科学依据。方法从疾病信息报告管理系统和流行病学调查资料分析寄生虫病特征。按照北京市人体重点寄生虫病现状调查方案,采用分层整群抽样方法抽取调查点,采用改良加藤厚涂片法检测虫卵,儿童用透明胶纸肛拭法检测蛲虫虫卵。随机抽取部分居民进行认知行为问卷调查。结果2011—2022年北京市通州区寄生虫病例26例,其中疟疾21例、血吸虫病2例、包虫病2例、华支睾吸虫病1例,平均发病率为0.15/10万,除华支睾吸虫病例外均为输入性病例。2015年调查1500人,土源性线虫、华支睾吸虫等感染率为0。2019年调查1015人,土源性线虫、华支睾吸虫等感染率为0。对319人进行了土源性线虫病问卷调查,知晓率为71.79%,对379人进行了华支睾吸虫病问卷调查,知晓率为34.56%,差异有统计学意义(χ^(2)=199.97,P<0.05)。结论北京市通州区寄生虫病例以输入性为主,人体重点寄生虫感染率处于较低水平,居民土源性线虫病知晓率较高,华支睾吸虫病知晓率较低,食源性寄生虫病有本地发病风险,应采取有针对性健康教育工作。随着外地和外籍病例到通州区就诊量的增多,对寄生虫病的培训、监测等防控工作始终不能放松。

关键词：人体重点寄生虫病感染率认知行为通州区

Trends in Suicide by Poisoning in China 2000-2006: Age, Gender, Method, and Geography

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Biomedical and Environmental Sciences 2008年第3期21卷 253-256页

作者： XING ZHANG HAI-SHAN li qiu-hong ZHU JING ZHOU SHUANG ZHANG liN ZHANG CHENG-YE SUN National Institute for Occupational Health and Poison Control Chinese Center for Disease Control and Prevention Beijing 100050 China

Objective This study analyzed patterns of suicide and suicide attempts by poisoning as reported through a national poison control system for the purpose of improving intervention and prevention. Methods During the period of 2000 to 2006, 6440 cases of poisoning suicide were reported to the telephone consultation service system of The National Center for Poisoning Control （Chinese Center for Disease Control and Prevention）. Among these records, 4728 cases had completed data for this analysis in terms of age, sex, trend of time and location, and type of poisons. Results There were 60.6% female cases with the age from 10 to 90 years old. The age of cases from 20 to 39 years accounted for 54.5% of all age groups. Both the numbers and percentage in record related to poisoning consultation of oral poisoning suicide showed an increasing tendency during the 7 years. In particular, there was a drastic increase from 2004 to 2006. In addition, the high frequency of cases occurred from May to October. Hebei, Shandong, Henan, and Anhui Provinces had the highest number of cases. Pesticide poisonings were the most common method in these cases of consultation for suicide and suicide attempts. Conclusion This study describes epidemiological characteristics in the oral poisoning suicide cases and provides scientific basis for suicide prevention interventions.

关键词： Suicide by poisoning Tendency Pesticide Intervene

Advances in metal-organic framework-derived single-atom catalysts for biomedicine

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Nano Materials Science 2024年第4期6卷 396-412页

作者： li qiu Yingxi lin Jun Song Chen hong Luo Rui Wu Department of Ultrasound West China Second University Hospital Sichuan University Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of Ministry of Education Sichuan University School of Materials and Energy University of Electronic Science and Technology of China Interdisciplinary Materials Research Center Institute for Advanced Study Chengdu University

As a alternative for natural enzymes, nanozymes has shown enzyme-like activity and selectivity in the field of various kinds of biomedical application, which has attracted considerable research interest. Recently, single-atom catalysts(SACs) have been extensively studied due to their similar active centers, coordination environment and better stability to natural enzymes. Metal-organic frameworks(MOFs) have been demonstrated as highly promising precursors for the synthesis of various types of SACs. MOF-derived SACs can not only significantly enhance the catalytic activity, but also improve the selectivity of nanozymes due to tunable coordination environment and structure, thereby receiving widespread attention in biomedicine. This review provided an overview of the preparation strategies for MOF-derived SACs, and then detailed the latest research progress of the SACs in the biomedical field for cancer, antibacterial, antioxidation and biosensors. Finally, the challenges and potential future opportunities of MOF-derived SACs in biomedical applications are proposed.

关键词： Biomedicine MOF-Derived SAC nanozymes Catalytic mechanisms

Synthesis and characterization on a novel series of protic pyrrolidinium surfactants

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Chinese Chemical Letters 2010年第4期21卷 385-387页

作者： Ma, Fu Min Chen, Xiao Wang, Xu Dong Zhao, Yu Rong li, qiu hong Yue, Xiu Lv, Chao Shandong Univ Minist Educ Key Lab Colloid & Interface Chem Jinan 250100 Peoples R China

A novel series of protic tertiary pyrrolidinium surfactants were prepared and characterized by different *** compounds show good conductivity and efficient ability to reduce surface *** analysis proves their high thermal stability at decomposition temperatures over 250℃.Their lyotropic and thermotropic liquid crystalline properties are also discussed.

关键词： Protic pyrrolidinium surfactants liquid crystal Conductivity Surface tension

Experimental Studies on Cyclooxygenase-2 Inhibitor Induced Cervical Cancer Hela Cell Apoptosis and Its Molecular Mechanism

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Journal of Reproduction and Contraception 2007年第4期18卷 271-277页

作者： ling YIN li-bei WEI qiu-hong QU Xiao-peng GUO Clinical College Wuhan University of Science and Technology Wuhan 430065 China

Objective To investigate the Hela cells growth inhibition and apoptosis possible molecular mechanisms. Methods Hela cells were treated with various concentrations （100μmol/L,200μmol/L, 300 μmol/L, 400 μmol/L） of NS-398 （selective for COX-2 inhibition）. Cell growth was measured by MTT （Thiazolyl blue）. Apoptosis was detected by double staining flow cytometry （FCM）. Levels of PGE： were measured by radioimmunoassay. The expressions of COX-2 protein were also examined by Western blot analysis. Results After treated with different concentrations of NS-398, the growth of Hela cells was suppressed significantly in a dose-and time-dependent manner （P〈0.01）. The NS-398 can induce apoptosis with the apoptosis rates at 8.53%-43.46% by FCM in a dose-dependent manner. The release of PGE2 was reduced in Hela cells with the values of 69.26 ± 2.13, 47.46 ± 2.18, 28.15 ± 1.64 and 17.01 ± 1.12, respectively, there was significant difference compared with control group （83.78 ± 1.11）（P〈0.01）. The NS-398 could inhibit the activity and expression of COX-2 in a dose- dependent manner and down-regulated the expression of COX-2 protein greatly. Conclusion NS-398 could inhibit the proliferation and increase apoptosis in human Hela cells. These effects may be depended on the inhibition of the expression of COX-2 and PGE2 by NS-398.

关键词： cyclooxygenase-2 NS-398 Hela cell apoptosis

慕课与Seminar文献研读联合教学法在牙体牙髓科住院医师规范化培训中的应用

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牙体牙髓牙周病学杂志 2024年第9期29卷 555-558页

作者：于雅琼薛明毕欣欣刘昕阳孙海燕仇丽鸿辽宁省口腔疾病重点实验室/中国医科大学口腔医学院/中国医科大学附属口腔医院牙体牙髓科辽宁沈阳110002 中国医科大学口腔医学院/中国医科大学附属口腔医院毕业后培训部辽宁沈阳110002

目的:探讨慕课与Seminar文献研读联合教学法在牙体牙髓科住院医师规范化培训(以下简称住培)中的作用。方法:以2021年01月至06月和2022年01月至06月的在中国医科大学附属口腔医院牙体牙髓科参加住培的40名住培学生为研究对象。其中,选择2... 详细信息

目的:探讨慕课与Seminar文献研读联合教学法在牙体牙髓科住院医师规范化培训(以下简称住培)中的作用。方法:以2021年01月至06月和2022年01月至06月的在中国医科大学附属口腔医院牙体牙髓科参加住培的40名住培学生为研究对象。其中,选择2021年的20名住培学生为对照组,采用传统教学法。2022年的20名住培学生为观察组,在传统教学模式基础上,增加慕课与Seminar文献研读联合教学法。比较两组住培学生的出科考核成绩,并进行教学满意度问卷调查。结果:观察组住培学生的出科考试理论成绩的平均分明显高于对照组(P<0.05),采用慕课与Seminar文献研读联合教学法的观察组住培生的住院医师岗位胜任力的多方面评价均高于采用传统教学方法的对照组住培学生(P<0.05)。结论:在牙体牙髓科住培中引入慕课与Seminar文献研读联合教学法能显著提高教学效果。

关键词：慕课 Seminar教学法牙体牙髓病学住院医师规范化培训文献研读