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RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

World Journal of Stem Cells 2024年第6期16卷 656-669页

作者： Daniel B Ferreira leticia m gasparoni Cristiane F Bronzeri Katiucia B S Paiva Laboratory of Extracellular matrix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

BACKGROUND Validation of the reference gene(RG)stability during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data ***,in an unreliable way,several studies use genes involved in essential cellular functions[glyceraldehyde-3-phosphate dehydro-genase(GAPDH),18S rRNA,andβ-actin]without paying attention to whether they are suitable for such experimental conditions or the reason for choosing such ***,such studies use only one gene when minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines recom-mend two or more *** impacts the credibility of these studies and causes dis-tortions in the gene expression *** tissue engineering,the accuracy of gene expression drives the best experimental or therapeutical *** cultivated DPSCs under two conditions:Undifferentiated and osteogenic dif-ferentiation,both for 35 *** evaluated the gene expression of 10 candidates for RGs[ribosomal protein,large,P0(RPLP0),TATA-binding protein(TBP),GAPDH,actin beta(ACTB),tubulin(TUB),aminolevulinic acid synthase 1(ALAS1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWHAZ),eukaryotic translational elongation factor 1 alpha(EF1a),succinate dehydrogenase complex,subunit A,flavoprotein(SDHA),and beta-2-micro-globulin(B2m)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analysed by the four main algorithms,ΔCt method,geNorm,NormFinder,and BestKeeper and ranked by the RefFinder *** subdivided the samples into eight *** All of the data sets from clonogenic and osteogenic samples were analysed using the RefFinder *** final ranking showed RPLP0/TBP as the two most stable RGs and TUB/B2m as the two least stable *** theΔCt method or NormFinder analysis showed TBP/RPLP0 as the two most stable ***,geNorm analysis showed RPLP0/EF1αin the first *** algorithms’two least stab

关键词： Dental pulp stem cells Reference gene Housekeeping gene Endogenous gene Osteogenic differentiation RefFinder

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Therapeutic potential of periodontal ligament stem cells

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World Journal of Stem Cells 2021年第6期13卷 605-618页

作者： Aline Queiroz Emmanuel Albuquerque-Souza leticia miquelitto gasparoni Bruno Nunes de França Cibele Pelissari marília Trierveiler marinella Holzhausen Laboratory of Stem Cell Biology in Dentistry-LABITRON Department of Oral and Maxillofacial PathologySchool of DentistryUniversity of São PauloSão Paulo 05508-000Brazil Department of Stomatology Division of PeriodonticsSchool of DentistryUniversity of São PauloSão Paulo 05508-000Brazil

Inflammatory periodontal disease known as periodontitis is one of the most common conditions that affect human teeth and often leads to tooth *** to the complexity of the periodontium,which is composed of several tissues,its regeneration and subsequent return to a homeostatic state is challenging with the therapies currently *** therapy is increasingly becoming an alternative in regenerative medicine/dentistry,especially therapies using mesenchymal stem cells,as they can be isolated from a myriad of *** ligament stem cells(PDLSCs)are probably the most adequate to be used as a cell source with the aim of regenerating the *** insights have also highlighted PDLSCs as promising immunomodulator *** this review,we explore the state of knowledge regarding the properties of PDLSCs,as well as their therapeutic potential,describing current and future clinical applications based on tissue engineering techniques.

关键词： Periodontal ligament stem cell mesenchymal stem cell Regenerative dentistry Therapeutics Immunology Cellular

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Precipitation kinetics of carbides during cyclical and isothermal aging of 2.25Cr–1mo steel and its effect on mechanical properties

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Journal of Iron and Steel Research International 2021年第10期28卷 1282-1290页

作者： maribel leticia Saucedo-muñoz ESIQIE Instituto Politécnico NacionalMexico07300Mexico

The carbide precipitation was analyzed during aging of 2.25Cr–1mo steel at 550℃.The as-received steel was aged in two different manners:the first treatment was an isothermal aging at 550℃ for time up to 1000 h,and the second one was carried out by a cyclical aging,which consisted of aging at 550℃ for 1 h,water quenching at room temperature and then newly heating at 550℃.This procedure was repeated up to 300 *** m_(23)C_(6) and m6C carbide precipitation took place intragranularly and intergranularly for both aging *** carbide coarsening was detected to occur with the increasing aging ***,the growth kinetics of precipitation occurred more rapidly in the case of cyclical *** specimen hardness decreased with the aging time in both cases;however,it occurred in shorter aging time for the cyclical *** testing indicated the increase in ductility with the aging time,and the cyclically aged specimens showed larger ductility than the isothermally aged specimens.

关键词： 2.25Cr-1mo steel Precipitation Isothermal heating Cyclical heating mechanical property

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The Damage Spreading method in monte Carlo Simulations:A Brief Overview and Applications to Confined magnetic materials

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Communications in Computational Physics 2008年第7期4卷 207-230页

作者： m.leticia Rubio Puzzo Ezequiel V.Albano Instituto de Investigaciones Fisicoquimicas Teoricas y Aplicadas(INIFTA) UNLPCONICETCasilla de Correo 16 Sucursal 4(1900)La PlataArgentina

The Damage Spreading(DS)method allows the investigation of the effect caused by tiny perturbations,in the initial conditions of physical systems,on their final stationary or equilibrium *** damage(D(t))is determined during the dynamic evolution of a physical system and measures the time dependence of the difference between a reference(unperturbed)configuration and an initially perturbed *** this paper we first give a brief overview of monte Carlo simulation results obtained by applying the DS *** model systems under study often exhibit a transition between a state where the damage becomes healed(the frozen phase)and a regime where the damage spreads arriving at a finite(stationary)value(the damaged phase),when a control parameter is finely *** kinds of transitions are actually true irreversible phase transitions themselves,and the issue of their universality class is also ***,the attention is focused on the propagation of damage in magnetic systems placed in confined *** influence of interfaces between magnetic domains of different orientation on the spreading of the perturbation is also discussed,showing that the presence of interfaces enhances the propagation of the ***,the critical transition between propagation and nonpropagation of the damage is *** all cases,the determined critical exponents suggest that the DS transition does not belong to the universality class of Directed Percolation,unlike many other systems exhibiting irreversible phase *** result reflects the dramatic influence of interfaces on the propagation of perturbations in magnetic systems.

关键词： Propagation of perturbations phase transitions and critical phenomena magnetic systems in confined geometries.

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Long-term stability in protected-areas?A vision from American/New World amphibians

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Geography and Sustainability 2024年第4期5卷 673-683页

作者： leticia m.Ochoa-Ochoa Julian A.Velasco Facultad de Ciencias Universidad Nacional Autónoma de MexicoCP 04510Mexico CityMexico Instituto de Ciencias de la Atmósfera y Cambio Climático Universidad Nacional Autónoma de MexicoCP 04510Mexico CityMexico

Protected areas(PA)have proven to be one of the best ways to conserve biodiversity against environmental *** are considered the most threatened group,with habitat loss due to deforestation identified as their major ***,we assessed for each PA of the American continent:1)amphibian’s occurrence(Global Biodiversity Information Facility(GBIF)*** Union for Conservation of Nature(IUCN)data);2)temperature velocity and estimated the climate residence time,and using the latest models of the land future use;3)we estimated the changes of natural *** cover in three future *** occurrence showed differences between databases,while GBIF data shows that 52%of the amphibian species occurring in the continent are in PA,based on IUCN data,85%are *** from climate change show a low pace of climate velocity during the last century that is maintained in the green scenario(SSP126).However,change in temperature increases in rate in the rest of the scenarios,with scenario SSP58 showing the highest velocity of temperature *** estimates of residence times in PA show that lower levels as emission scenarios tend to be *** results are worrisome since climate lag,specifically temperature increase over the PA will probably affect amphibian communities as shown in previous *** in climate patterns have a direct—mostly negative—impact on amphibians’ability to disperse and *** results of land use change were unexpected,since the categories showed minimal ***,the data on urbanization changes do not seem to be reflecting the trends of other databases,which may be causing artifacts in the comparisons in the future models of land *** research will be necessary to evaluate the extent of similarities and differences in future projections of land use including urbanization and human population between different databases.

关键词： Climate lag Conservation Future land use models Governance Temperature tendency

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Novel strategies to tailor the photocatalytic activity of metal–organic frameworks for hydrogen generation: a mini-review

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Frontiers in Energy 2022年第5期16卷 734-746页

作者： Luis A.ALFONSO-HERRERA leticia m.TORRES-mARTINEZ J.manuel mORA-HERNANDEZ Universidad Autónoma de Nuevo León UANLFacultad de Ingeniería CivilDepartamento de Ecomateriales y EnergíaAv.Universidad S/N Ciudad UniversitariaSan Nicolás de los GarzaNuevo León 66455México Centro de Investigación en materiales Avanzados S.C.(CIMAV)Miguel de Cervantes No.120.Complejo Ind.ChihuahuaChihuahuaChih 31136México CONACYT-Universidad Autónoma de Nuevo León UANLFacultad de Ingeniería CivilDepartamento de Ecomateriales y EnergíaAv.Universidad S/N Ciudad UniversitariaSan Nicolás de los GarzaNuevo León 66455México

This review provides a recompilation of the most important and recent strategies employed to increase the efficiency of metal-organic framework(mOF)-based systems toward the photocatalytic hydrogen evolution(PHE)reaction through specific strategies:tailoring the photocatalytic activity of bare mOFs and guest@mOF composites,formation of heterojunctions based on mOFs and various photocatalysts,and inorganic photocatalysts derived from *** to the data reported in this mini-review,the most effective strategy to improve the PHE of mOFs relies on modifying the linkers with new secondary building units(SBUs).Although several reviews have investigated the photocatalytic activity of mOFs from a general point of view,many of these studies relate this activity to the physicochemical and catalytic properties of ***,they did not consider the interactions between the components of the photocatalytic *** study highlights the effects of strength of the supramolecular interactions on the photocatalytic performance of bare and mOF-based materials during PHE.A thorough review and comparison of the results established that metal-nanoparticle@mOF composites have weak van der Waals forces between components,whereas heterostructures only interact with mOFs at the surface of bare *** material derivatives from mOFs,we found that pyrolysis destroyed some beneficial properties of mOFs for ***,we conclude that adding SBUs to organic linkers is the most efficient strategy to perform the PHE because the SBUs added to the mOFs promote synergy between the two materials through strong coordination bonds.

关键词： metal-organic frameworks(mOFs) photocatalytic hydrogen evolution mOF heterojunctions materials derived from mOFs bandgap recombination

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Reproduction ecology of the recently invasive snake Hemorrhois hippocrepis on the island of Ilbiza Elba

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Current Zoology 2020年第4期66卷 363-371页

作者： Elba moNTES monica FERICHE leticia Ruiz-SUEIRo Esmeralda ALAmINos Juan m.Pleguezuelos Department of Zoology Faculty of Biological SciencesUniversity of Valenciac/Dr.Moliner50BurjassotValencia E-46100Spain Department of Zoology Faculty of SciencesGranada UniversityGranada E-18071Spain Laboratory of Ecology and Evolution Butantan InstituteUniversity of Sao PauloAv.Vital Brazil1.500ButantaE-05503900Sao PauloBrasil Department of Sciences Granada Sciences ParkGranadaSpain*Address correspondence to Elba Montes

Knowing the causes of biological invasion success can be relevant to combat future invasive *** recent invasion of the horseshoe whip snake Hemorrhois hippocrepis on the island of Ibiza provides the opportunity to compare natural history traits between invasive and source populations,and to unravel what makes this snake a successful invader that is threatening the only endemic vertebrate of the island,Podarcis *** study compares the basic reproductive traits of mainland native and invasive populations of the *** results revealed that invasive populations were characterized by female maturity at a smaller size,extended reproductive period,and much lower reproduction frequency compared to the native *** contrast,some major reproductive traits--the abdominal fat body cycle,clutch size,hatchling body size,and hatchling body condition,did not differ between the two *** of these results must reflect the environmental differences in the recently invaded island with respect to the source area,and overall plasticity of reproductive *** is evolutionarily interesting,and may aid the succesful growth of this species in their invasiveness of mediterranean islands like *** most significant finding is that this expression of phenotypic plasticity occurred rapidly in this invasive population,within a period of 14 years *** results on the reproduction ecology of the invasive population were not conclusive regarding the factors determining the invasiveness of the snake and pointed to alternative causes.

关键词： lbiza invasion sucess islands reproduction frequency.snakes

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The importance of glutathione and phytochelatins on the selenite and arsenate detoxification in Arabidopsis thaliana

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Journal of Environmental Sciences 2016年第11期28卷 150-161页

作者： Fatai Adigun Aborode Andrea Raab matthias Voigt leticia malta Costa Eva m.Krupp Joerg Feldmann Trace Element Spedation Laboratory Department of Chemistry University of Aberdeen Aberdeen AB24 3UE Scotland UK Departamento de Quimica Uniuersidade Federal de Minas Gerais Belo Horizonte MG CEP： 31270-901 Brazil

We investigated the role of glutathione（GSH） and phytochelatins（PCs） on the detoxification of selenite using Arabidopsis thaliana. The wild-type（WT） of Arabidopsis thaliana and its mutants（glutathione deficient Cad 2–1 and phytochelatins deficient Cad 1–3） were separately exposed to varying concentrations of selenite and arsenate and jointly to both toxicants to determine their sensitivities. The results of the study revealed that, the mutants were about 20-fold more sensitive to arsenate than the WT, an indication that the GSH and PCs affect arsenate detoxification. On the contrary, the WT and both mutants showed a similar level of sensitivity to selenite, an indication that the GSH and PCs do not significantly affect selenite detoxification. However, the WT is about 8 times more sensitive to selenite than to arsenate, and the mutants were more resistant to selenite than arsenate by a factor of 2. This could not be explained by the accumulation of both elements in roots and shoots in exposure experiments. The co-exposure of the WT indicates a synergistic effect with regards to toxicity since selenite did not induce PCs but arsenic and selenium compete in their PC binding as revealed by speciation analysis of the root extracts using HPLC–ICP–mS/ESI–mS. In the absence of PCs an antagonistic effect has been detected which might suggest indirectly that the formation of Se glutathione complex prevent the formation of detrimental selenopeptides. This study, therefore, revealed that PC and GSH have only a subordinate role in the detoxification of selenite.

关键词： Toxicity Phytochelatins Glutathione Selenopeptides ICP–mS ESI–mS Arabidopsis thaliana

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Effect of the addition of different sodium alginates on viscoelastic,structural features and hydrolysis kinetics of corn starch gels

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Food Bioscience 2022年第3期47卷 271-278页

作者： leticia montes maria Santamaria Raquel Garzon Cristina m.Rosell Ramón moreira Department of Chemical Engineering Universidade de Santiago de Compostelarúa Lope Gómez de Marzoas/n15782Santiago de CompostelaSpain Institute of Agrochemistry and Food Technology (IATA-CSIC).C/Agustin Escardino 746980PaternaSpain

Corn starch gels(1:4 w:w)were made with the addition(up to 2%w/w starch basis)of sodium alginates of different average molecular sizes and *** of the starchy gels were characterized to evaluate their *** were subjected to in vitro digestion to estimate the effect of alginates addition on glycemic *** alginates significantly decreased the value of the elastic modulus of *** coordination number obtained from complex modulus indicated that in general,less compact structures were obtained in the presence of *** results were confirmed by scanning electron micrographs,where significant differences in the structure of alginate-corn starch gels were observed,but molecular size of alginates was not *** rate decreased with the presence of alginates in corn starch gels by interactions of alginates with leached *** high alginate content,slower hydrolysis rates were determined when the most hygroscopic alginates were added.

关键词： Biopolymers In vitro digestion Average molecular weight Rheology Scanning electron microscopy(SEm)

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Applications of endoscopic ultrasound in pancreatic cancer

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World Journal of Gastroenterology 2014年第24期20卷 7808-7818页

作者： leticia Perondi Luz mohammad Ali Al-Haddad michael Sai Lai Sey John m DeWitt Division of Gastroenterology and Hepatology Indiana UniversityMedical CenterRoudebush VA Medical CenterIndianapolisIN 46202United States

Since the introduction of endoscopic ultrasound guided fine-needle aspiration(EUS-FNA),EUS has assumed a growing role in the diagnosis and management of pancreatic ductal adenocarcinoma(PDAC).The objective of this review is to discuss the various applications of EUS and EUS-FNA in ***,its use for detection,diagnosis and staging will be *** and EUS-FNA are highly accurate modalities for detection and diagnosis of PDAC,this high accuracy,however,is decreased in specific situations particularly in the presence of chronic *** techniques such as contrast-enhanced EUS,elastography and analysis of DNA markers such as k-ras mutation analysis in FNA samples are in progress and might improve the accuracy of EUS in the detection of PDAC in this setting and will be *** and EUS-FNA have recently evolved from a diagnostic to a therapeutic technique in the management of *** developments in therapeutic EUS have occurred including advances in celiac plexus interventions with direct injection of ganglia and improved pain control,EUS-guided fiducial and brachytherapy seed placement,fine-needle injection of intra-tumoral agents and advances in EUS-guided biliary *** future role of EUS and EUS in management of PDAC is still emerging.

关键词： Pancreatic ductal carcinoma Pancreatic neoplasm Endoscopic Ultrasound-Guided Fine Needle Aspiration Endosonography Endoscopic ultrasound guided fine-needle aspiration

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