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RPLP0/TBP are the most stable reference genes for human dental pulp stem cells under osteogenic differentiation

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World Journal of Stem Cells 2024年第6期16卷 656-669页

作者： Daniel B ferreira leticia M Gasparoni Cristiane F Bronzeri Katiucia B S Paiva Laboratory of Extracellular Matrix Biology and Cellular Interaction Department of AnatomyInstitute of Biomedical SciencesUniversity of São PauloSão Paulo 05508-000São PauloBrazil

BACKGROUND Validation of the reference gene(RG)stability during experimental analyses is essential for correct quantitative real-time polymerase chain reaction(RT-qPCR)data ***,in an unreliable way,several studies use genes involved in essential cellular functions[glyceraldehyde-3-phosphate dehydro-genase(GAPDH),18S rRNA,andβ-actin]without paying attention to whether they are suitable for such experimental conditions or the reason for choosing such ***,such studies use only one gene when Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines recom-mend two or more *** impacts the credibility of these studies and causes dis-tortions in the gene expression *** tissue engineering,the accuracy of gene expression drives the best experimental or therapeutical *** cultivated DPSCs under two conditions:Undifferentiated and osteogenic dif-ferentiation,both for 35 *** evaluated the gene expression of 10 candidates for RGs[ribosomal protein,large,P0(RPLP0),TATA-binding protein(TBP),GAPDH,actin beta(ACTB),tubulin(TUB),aminolevulinic acid synthase 1(ALAS1),tyro-sine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta(YWHAZ),eukaryotic translational elongation factor 1 alpha(EF1a),succinate dehydrogenase complex,subunit A,flavoprotein(SDHA),and beta-2-micro-globulin(B2M)]every 7 d(1,7,14,21,28,and 35 d)by *** data were analysed by the four main algorithms,ΔCt method,geNorm,NormFinder,and BestKeeper and ranked by the RefFinder *** subdivided the samples into eight *** All of the data sets from clonogenic and osteogenic samples were analysed using the RefFinder *** final ranking showed RPLP0/TBP as the two most stable RGs and TUB/B2M as the two least stable *** theΔCt method or NormFinder analysis showed TBP/RPLP0 as the two most stable ***,geNorm analysis showed RPLP0/EF1αin the first *** algorithms’two least stab

关键词： Dental pulp stem cells Reference gene Housekeeping gene Endogenous gene Osteogenic differentiation RefFinder

Spatial-temporal distribution of fatal yellowing in different oil palm genetic materials in eastern amazon

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Information Processing in Agriculture 2022年第3期9卷 365-377页

作者： Bruno Borella Anhe Artur Vinicius ferreira dos Santos Thiago Alan ferreira da Silva Lana leticia Barbosa de Carvalho Paulo Roberto Silva Farias Universidade Federal Rural da Amazonia Geoestatistics Department66.077-830 BelemBrazil Universidade Federal Rural da Amazonia Entomology Department66.077-830 BelemBrazil

Oil palm (Elaeis guineensis Jacq.) is one of the agricultural crops with the greatest potentialfor vegetable oil production in Brazil. However, a disease of unknown etiology popularlyknown as Fatal Yellowin (FY) has caused damage to Brazilian farmers particularly in theeastern region of the Amazon. So, the objective of this study was to evaluate the spatialdependence of FY on three oil palm genotypes, grown for many years in an organic produc-tion system in the Amazon region. The study area had 4104 ha, divided into 139 plots. Ineach plot, the monthly incidence of disease was monitored forming a database. The num-ber of diseased plants per year, number of accumulated diseased plants, number of dis-eased plants per hectare, growth rate of diseased plants and incidence of accumulateddisease were evaluated. The results indicated spatial distribution of the variables adjustedto the gaussian, spherical and exponential models, with predominance of the first *** increases the purpose that FY is caused by biotic factors. The highest range achievedin the study was 2929 m indicating the susceptibility of the tested genotypes. Some plotsclose to the river had the highest incidence of the disease on the study, probably associatedwith higher soil moisture.

关键词： Elaeis guineensis Geostatiscal analysis Kriging Palm oil Spatial dependence

Rat Hepatocyte Glucose Metabolism Is Affected by Caloric Restriction but not by Litter Size Reduction

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Journal of Pharmacy and Pharmacology 2017年第7期5卷 408-415页

作者： Nayra Thais Delatorre Branquinho Gabriel Henrique de Paula Cruz Arielle da Cunha Silvéio leticia Diniz repaldi Lais Akemi Yamada Isabela Ramos Mariano Vilma Aparecida ferreira de Godoi Maria ontserrat Diaz Pedrosa Program of Graduate Studies in Biological Sciences State University of Maringá Maringá 87020-900 Brazil Undergraduation in Biological Sciences State University of Maringá Maringá 87020-900 Brazil Undergraduation in Biotechnology State University of Maringá Maringá 87020-900 Brazil Department of Physiological Sciences State University of Maringá Matingá 87020-900 Brazil

COVID-19: Feelings Experienced by Nurses, Physiotherapists, and Medical Doctors in a Brazilian Field Hospital

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Open Journal of Nursing 2021年第5期11卷 390-406页

作者： Joselito Adriano da Silva Giulia Catissi leticia Bernardes de Oliveira Daniela Reis Dal Fabbro Michele Jaures Elisiane Plack Gladys Cristina Borges Fábio Racy Andreia ferreira Nunes Rose Mara Miranda Denise Tiemi Noguchi Maki Guilherme de Paula Pinto Schettino Claudia Regina Laselva Eliseth Ribeiro Leão Hospital Israelita Albert Einstein S&#227 o Paulo Brazil

Facing critical situations such as the ones triggered by COVID-19 may take healthcare professionals to confront their psychological resources, which may induce a higher stress level. We aimed to understand the feelings experienced by nurses, physiotherapists, and medical doctors in a field hospital dedicated to patients with COVID-19 in the biggest city of Brazil. Methods: We performed a qualitative study in a state field hospital in S?o Paulo city, Brazil, in a sample of 25 volunteer healthcare professionals. Participants answered to a sociodemographic electronic survey and were subjected to an individual semi-structured on-site interview. Results: Participants were mostly female (68%), comprised eight nurses, eight physiotherapists, and nine medical doctors, had an average age of 36.8 years, and were professionally experienced. Speech analysis revealed two thematic categories (related to work and assistance in the pandemic) and 11 subcategories: adaptation to the scenario, stressing experience, psychological support, difficulties with workwear, safety with workwear, non-verbal communication, empathy, patient comfort, outstanding experiences, major challenges, and teamwork. Conclusion: Nurses, physiotherapists, and medical doctors’ experiences while working in the field hospital were permeated by both positive and negative feelings. Those feelings originated directly from the fear of the unknown when dealing with a new disease without well-established scientific evidence, the use of medical workwear for long periods, and communication and environmental difficulties. In contrast, positive feelings, related to teamwork and empathy, arose and overlapped with the difficulties.

关键词： Covid-19 Pandemic Healthcare Professionals Field Hospital

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

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Agricultural Sciences 2015年第3期6卷 295-303页

作者： leticia Sayuri Murate Admilton Goncalves de Oliveira Allan Yukio Higashi Andre Riedi Barazetti Ane Stefano Simionato Caroline Santos da Silva Glenda Cavalari Simoes Igor Matheus Oliveira dos Santos Marlon Renan ferreira Martha Viviana Torres Cely Miguel Octavio Pérez Navarro Vanessa Fogaca de Freitas Marco Antonio Nogueira Joao Carlos Palazzo de Mello Rui Pereira Leite Jr. Galdino Andrade Department of Microbiology Londrina State UniversityLondrinaBrazil Embrapa Soja LondrinaBrazil Department of Pharmacy and Pharmacology Maringa State UniversityMaringáBrazil Plant Protection Agronomic Institute of ParanáIAPARLondrinaBrazil

This study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions’ activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrationswere tested. Cytotoxicity effects were observed in all fractions in 50 μg·mL-1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p<0.05), reducing the incidence of citrus canker lesions.

关键词： X.axonopodis pv.citri Citrus sinensis Antibiosis Biological Control Natural Antibiotic

Caloric Restriction and Litter Size Do Not Compromise Renal Function of Male Wistar Rats

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Journal of Pharmacy and Pharmacology 2018年第4期6卷 362-369页

作者： Nayra Thais Delatorre Branquinho leticia Diniz Crepaldi Vilma Aparecida ferreira de Godoi Maria Montserrat Diaz Pedrosa Edmara Aparecida Baroni Graduation (PhD.) in Cell and Molecular Biology State University of Matinga Matinga 87020-900 Brazil Graduation (Msc.) in Cell and Molecular Biology State University of Maringa Maringa 87020-900 Brazil Associate Professor Department of Physiological Sciences State University of Maringa Maringa 87020-900 Brazil

This work had the purpose of comparing some renal parameters of Wistar rats raised in small litters either freely-fed (G3L) or subjected to 30% caloric restriction (G3R) with those of rats from normal litters (G9) at the age of 90 days. The results showed that the relative weight of the kidneys and urinary flow were not significantly different. All the groups had GFR (glomerular filtration rate) and proteinuria within normal values, despite group G3R having values statistically lower. However, group G3R had diminished glomerular area and increased numbers of renal corpuscles. Group G3L did not exhibit differences compared with controls (G9). The effects of caloric restriction did not compromise kidney structure and function in 90-day-old rats.

关键词： Wistar 老鼠热量损害肾尺寸相对重量价值