检索结果-南通市图书馆

Virologica Sinica 2022年第3期37卷 341-347页

作者： Lei Yang lingqian Tian leshan li Qiuhong liu Xiang Guo Yuan Zhou Rongjuan Pei Xinwen Chen Yun Wang State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

Transformation-associated recombination(TAR)has been widely used to assemble large DNA *** of the significant obstacles hindering assembly efficiency is the presence of error-prone DNA repair pathways in yeast,which results in vector backbone recircularization or illegitimate recombination *** increase TAR assembly efficiency,we prepared a dual-selective TAR vector,pGFCS,by adding a PADH1-URA3 cassette to a previously described yeast-bacteria shuttle vector,p GF,harboring a PHIS3–HIS3 cassette as a positive selection *** new cassette works as a negative selection marker to ensure that yeast harboring a recircularized vector cannot propagate in the presence of 5-fluoroorotic *** prevent pGFCS bearing ura3 from recombining with endogenous ura3-52 in the yeast genome,a highly transformable Saccharomyces cerevisiae strain,VL6-48B,was prepared by chromosomal substitution of ura3-52 with a transgene conferring resistance to blasticidin.A55-kb genomic fragment of monkeypox virus encompassing primary detection targets for quantitative PCR was assembled by TAR using pGFCS in *** pGFCS-mediated TAR assembly showed a zero rate of vector recircularization and an average correct assembly yield of 79%indicating that the dual-selection strategy provides an efficient approach to optimizing TAR assembly.

关键词： Monkeypox virus Transformation-associated recombination (TAR) TAR assembly

Comparison of viral propagation and drug response among SARS-CoV-2 VOCs using replicons capable of recapitulating virion assembly and release

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2022年第5期37卷 695-703页

作者： lingqian Tian Qiuhong liu Rongjuan Pei Yingshan Chen Chonghui Xu Jielin Tang Hao Sun Kunpeng liu Qi Yang Lei Yang leshan li Yongli Zhang Yuan Zhou Chao Shan Xue Hu Xinwen Chen Yun Wang State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China Department of Gastroenterology Guangzhou Women and Children's Medical CenterGuangzhou510623China Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou510530China Innovation Center for Pathogen Research Guangzhou LaboratoryGuangzhou510320China

Several variants of concern(VOCs)have emerged since the WIV04 strain of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)was first isolated in January *** to mutations in the spike(S)protein,these VOCs have evolved to enhance viral infectivity and immune ***,whether mutations of the other viral proteins lead to altered viral propagation and drug resistance remains *** replicon is a noninfectious viral surrogate capable of recapitulating certain steps of the viral life *** several SARS-CoV-2 replicons have been developed,none of them were derived from emerging VOCs and could only recapitulate viral genome replication and subgenomic RNA(sgRNA)*** this study,SARS-CoV-2 replicons derived from the WIV04 strain and two VOCs(the Beta and Delta variants)were prepared by removing the S gene from their genomes,while other structural genes remained *** replicons not only recapitulate viral genome replication and sgRNA transcription but also support the assembly and release of viral-like particles,as manifested by electron microscopic ***,the S-deletion replicon could recapitulate virtually all the post-entry steps of the viral life cycle and provides a versatile tool for measuring viral intracellular propagation and screening novel antiviral drugs,including inhibitors of virion assembly and *** the quantification of replicon RNA released into the supernatant,we demonstrate that viral intracellular propagation and drug response to remdesivir have not yet substantially changed during the evolution of SARS-CoV-2 from the WIV04 strain to the Beta and Delta VOCs.

关键词： SARS-CoV-2 Variants of concern(VOC) Viral-like particle(VLP) Replicon Remdesivir

在线全文

学校读者我要写书评

暂无评论

热力学函数简化式参数的加工

计算机与应用化学 1986年第3期 215-218页

作者：王乐珊李燕燕中国科学院化工冶金研究所

本文介绍在无机热化学数据库(ITDB)中根据用户的要求而新开发的第16个功能,即加工热力学函数计算的简化式F=A+BT之参数的方法,并给出一些实例。

关键词：热力学函数热力学性质热化学数据无机物 OK 温度范围等压热容化学反应平衡常数自由能计算

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

自然资本的内涵及其核算研究

生态经济 2018年第4期34卷 153-157,163页

作者：刘高慧胡理乐高晓奇杜乐山李俊生肖能文中国环境科学研究院生物多样性研究中心

我国国土面积广阔，自然资源丰富，根据目前的研究理论，绝大多数自然资源均具有价值，可转化为自然资本，作为国家财富的一部分。然而，目前人类对自然资本的概念及其自然资源、自然资源资产、生态系统服务等相关概念之间的关系仍不甚... 详细信息

我国国土面积广阔，自然资源丰富，根据目前的研究理论，绝大多数自然资源均具有价值，可转化为自然资本，作为国家财富的一部分。然而，目前人类对自然资本的概念及其自然资源、自然资源资产、生态系统服务等相关概念之间的关系仍不甚明了。文章梳理并比较分析了学术界及各大组织和机构对自然资本的定义，总结概括出适应中国现状的自然资本的概念，并对自然资本相关概念进行辨析，最后就中国自然资本核算及自然资源资产负债表编制工作提出了建议。

关键词：自然资本自然资源资产价值核算

Taxonomic circumscription of Sinojackia xylocarpa (Styracaceae)

在线全文

学校读者我要写书评

暂无评论

Journal of Systematics and Evolution 2011年第2期49卷 163-164页

作者： 1 li-Qun LUO 2 Chi LUO 1 (Department of Chemistry and life Science, leshan Normal University, leshan 614004, China) 2 (Department of Mathematics, leshan Normal University, leshan 614004, China) (Department of Chemistry and life Science Leshan Normal University Leshan 614004 China) (Department of Mathematics Leshan Normal University Leshan 614004 China)

Sinojackia xylocarpa Hu in Journ. Arn. Arb. 9: 130. 1928. Type: China. Jiangsu: Nanjing, Y. L. Keng 1376 (PE!). Sinojackia rehderiana Hu in Journ. Arn. Arb. 11: 227. 1930, syn. nov. Type: China. Jiangxi: Nanchang, H. ... 详细信息

关键词： PE Styracaceae Taxonomic circumscription of Sinojackia xylocarpa

慢性前列腺炎患者信息需求与支持需求调查研究

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

护理学杂志 2009年第8期24卷 42-44页

作者：雷芬芳邓翠珍尹冠群唐怡涛李乐山邵阳医学高等专科学校护理系湖南邵阳422000 邵阳医学高等专科学校附属医院泌尿外科湖南邵阳422000 邵阳市中心医院泌尿外科

目的探讨慢性前列腺炎(CP)患者信息与支持需求,为临床开展有效护理干预提供依据。方法采用症状自评量表、自制慢性前列腺炎信息需求与支持需求调查表,对186例慢性前列腺炎患者进行调查。结果各需求因素得分排前5位的是:性生活指导、疾... 详细信息

目的探讨慢性前列腺炎(CP)患者信息与支持需求,为临床开展有效护理干预提供依据。方法采用症状自评量表、自制慢性前列腺炎信息需求与支持需求调查表,对186例慢性前列腺炎患者进行调查。结果各需求因素得分排前5位的是:性生活指导、疾病预防措施、医生心理指导、配偶支持、疾病反复难治的原因。30～岁组、本科及以上学历、病程1～2年、局部加全身症状组信息与支持需求最高(均P<0.05)。结论CP患者信息需求较高,应重视患者需求及护理干预重点人群,采取个性化的心理指导,帮助患者建立良好的行为方式,调动家庭支持系统的作用,以促进患者康复。

关键词：慢性前列腺炎信息需求支持需求数据收集

livelihood Diversification of Farm Households and Its Impact on Cultivated Land Utilization in Agro-pastoral Ecologically-vulnerable Areas in the Northern China

在线全文

学校读者我要写书评

暂无评论

Chinese Geographical Science 2020年第2期30卷 279-293页

作者： liU Haiyan HAO Haiguang HU Xujun DU leshan ZHANG Zhe li Yuanyuan State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental SciencesBeijing 100012China

The relationship between livelihood diversification of farm households and cultivated land utilization has become a core research topic related to global environmental ***-pastoral ecologically-vulnerable areas face challenges such as insufficient ecosystem conservation,low agricultural production,and weak *** this study,215 farm households from Zhengxiangbai Banner,Taibus Banner,and Duolun County of Inner Mongolia were *** sustainable livelihoods framework of the United Kingdom(UK)Department for International Development(DFID)was used to measure the livelihood capital of these farm households.A one-way analysis of variance(ANOVA)was applied to examine the differences in the livelihood capital of different types households,and a correlation analysis was applied to analyze its impact on cultivated land *** showed that households with non-farming activities accounted for 64.7%of the total surveyed households,and non-farming employment was becoming more *** and financial capital was the driving factors for livelihood *** livelihood capital had key factors that affected household farmland use behaviors,such as the age of householder,the labor ratio,proportion of income,farmland scale,number of machines,and these had a significantly positive or negative influence on farmland ***-time farming households were more likely to transfer the land into cultivation and invest more labor,while non-farming households with high income were likely to transfer farmland out and invest more money to develop efficient farming or improve the employment *** results of this study suggest that policymakers need to fully consider livelihood changes of local *** is effective to strengthen labor training,create farmland market and improve the efficiency of farmland *** hope to achieve a win-win scenario to improve local economies and ecosystem conservation.

关键词： agro-pastoral ecologically-vulnerable areas livelihood diversification cultivated land utilization ecosystem conservation

Determining antimicrobial resistance profiles and identifying novel mutations of Neisseria gonorrhoeae genomes obtained by multiplexed MinION sequencing

在线全文

学校读者我要写书评

暂无评论

Science China(life Sciences) 2020年第7期63卷 1063-1070页

作者： Chi Zhang Feng Wang Cansheng Zhu leshan Xiu Yamei li li li Bo liu Yizhun li Yaling Zeng Boyang Guo Junping Peng NHC Key Laboratory'of Systems Biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100176China Shenzhen Center for Chronic Disease Control Shenzhen 518020China Department of STD Laboratory Shaanxi Provincial Institute for Skin Disease and STD ControlXi'an 710003China

Gonorrhea is one of the most common sexually transmitted diseases worldwide. To cure infection and prevent transmission,timely and appropriate antimicrobial therapy is necessary. Unfortunately, Neisseria gonorrhoeae, the etiological agent of gonorrhea, has acquired nearly all known mechanisms of antimicrobial resistance(AMR), thereby compromising the efficacy of antimicrobial therapy. Treatment failure resulting from AMR has become a global public health concern. Whole-genome sequencing is an effective method to determine the AMR characteristics of N. gonorrhoeae. Compared with next-generation sequencing, the MinION sequencer(Oxford Nanopore Technologies(ONT)) has the advantages of long read length and portability. Based on a pilot study using MinION to sequence the genome of N. gonorrhoeae, we optimized the workflow of sequencing and data analysis in the current study. Here we sequenced nine isolates within one flow cell using a multiplexed sequencing strategy. After hybrid assembly with Illumina reads, nine integral circular chromosomes were obtained. By using the online tool Pathogenwatch and a BLAST-based workflow, we acquired complete AMR profiles related to seven classes of antibiotics. We also evaluated the performance of ONT-only assemblies. Most AMR determinants identified by ONT-only assemblies were the same as those identified by hybrid assemblies. Moreover, one of the nine assemblies indicated a potentially novel antimicrobial-related mutation located in mtrR which results in a frame-shift, premature stop codon, and truncated *** addition, this is the first study using the MinION sequencer to obtain complete genome sequences of N. gonorrhoeae strains which are epidemic in China. This study shows that complete genome sequences and antimicrobial characteristics of *** can be obtained using the MinION sequencer in a simple and cost-effective manner, with hardly any knowledge of bioinformatics required. More importantly, this strategy provid

关键词： nanopore sequencing MinION sequencer Neisseria gonorrhoeae whole-genome sequencing antimicrobial resistance

Research on Human Resources Development of Rural Public Service Departments in New Countryside Construction

在线全文

学校读者我要写书评

暂无评论

Asian Agricultural Research 2011年第8期3卷 41-44页

作者： li Zhong-dong leshan Vocational and Technical College,leshan 614000,China

On the basis of defining related concepts concerning human resources in rural public service departments,this paper analyses the overall status quo of human resources in China's rural public service departments and points out the problems existing in human resources in China's rural public service departments during new countryside construction as follows:first,the constitution structure is not rational;second,the cultural quality of staff is universally low,and the business capacity remains to be promoted;third,the improvement of human resources development environment lags behind,and the supporting reform is *** the context of new countryside construction,the opportunities faced by the human resources in China's rural public service departments are as follows:China has elevated strengthening new countryside construction and rural talents construction as important state development strategy;the ideas of service-oriented government and learning-oriented government are put forward;civil servant system is ***,I advance the development strategy of human resources in China's rural public service departments as follows:implement elastic personnel system reform in public service departments(including civil servant positions in department),to form the public service personnel system of "able one comes in,mediocre one moves over and shiftless one steps down";audaciously promote young and middle-aged grass-roots cadres with strong business ability,high political quality,acute judgement and decision-making ability;build learning-oriented grass-roots public service organization,to make the staff in rural pubic service departments study assiduously and progress;broaden horizon,and build the human resources development system geared to international standards;in developed regions,the human resources in grass-roots public service departments should introduce enterprise competition model.

关键词： New countryside construction Rural public service

Optimization of the Recommended Time for Rectal Temperature Measurement in Children: An Observational Study

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Pediatrics 2019年第2期9卷 139-147页

作者： Qian Zhao Yanwei li leshan Zhou Nursing School of Central South University Changsha China Hunan Provincial Research Center for Women and Children Health Changsha China Pediatric Department Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Background: This study aimed to determine the optimum time required to measure rectal temperature in children with mercury-in-glass thermometers. Methods: This cross-sectional observational study involved a random sample of pediatric patients ≤5 years of age. Body temperature was measured for 3 - 5 minutes using standard mercury-in-glass rectal thermometers. Outcomes were rectal body temperatures at 1, 2, and 3 minutes until reaching a stable rectal temperature, and the final rectal temperature. Results: This study recruited 120 children. Mean time to reach a stable rectal temperature was 1.8 minutes (range: 30 seconds to five minutes). 90% of pediatric patients’ temperature came out within ±0.1°C of the final temperature at two minutes. There was no correlation between the time taken to reach a stable rectal temperature and age, body weight, gender, or the final temperature. Conclusion: Mercury-in-glass thermometers can be used to obtain accurate rectal temperature measurements at two minutes in routine pediatric practice.

关键词： Fever Mercury Thermometer Pediatrics Rectal Temperature