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Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang Xin Liu Jian Zhang Ya Gao Shuyue Li Cui Chang xiang Liu Guofeng Yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-Yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

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司莫拉少数民族村寨旅游对佤族居民主观幸福感影响——基于居民旅游感知视角

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云南农业大学学报（社会科学版） 2025年第1期19卷 167-176页

作者：金磊高翔云南农业大学经济管理学院云南昆明650201 云南农业大学学生处云南昆明650201

为深入探讨少数民族村寨旅游发展与少数民族传统村落居民主观幸福感的关系,本文以云南腾冲司莫拉佤族村为研究案例,构建基于居民旅游感知的主观幸福感影响的实证研究。研究发现:(1)司莫拉当地佤族居民旅游感知主要包括经济、文化和环境... 详细信息

为深入探讨少数民族村寨旅游发展与少数民族传统村落居民主观幸福感的关系,本文以云南腾冲司莫拉佤族村为研究案例,构建基于居民旅游感知的主观幸福感影响的实证研究。研究发现:(1)司莫拉当地佤族居民旅游感知主要包括经济、文化和环境的正负面效应六个维度。(2)当地佤族居民对旅游正面效应的感知显著高于负面效应,尤其是旅游经济正面效应和环境正面效应对其主观幸福感的提升作用显著,而文化负效应对幸福感有显著的负向影响。基于上述研究结果和当地发展的实际,针对推动当地旅游业可持续发展和提升居民幸福感给出三点建议:(1)丰富居民旅游参与方式,加深居民旅游参与程度,夯实居民幸福基础。(2)增强文化认同感、规范文明行为、构建和谐的旅游发展环境。(3)推动基建升级,加强生态保育,走绿色旅游之路。

关键词：乡村振兴少数民族村寨旅游居民旅游感知居民主观幸福感

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Resting-state brain network remodeling after different nerve reconstruction surgeries:a functional magnetic resonance imaging study in brachial plexus injury rats

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Neural Regeneration Research 2025年第5期20卷 1495-1504页

作者： Yunting xiang xiangxin Xing Xuyun Hua Yuwen Zhang Xin Xue Jiajia Wu Mouxiong Zheng He Wang Jianguang Xu School of Rehabilitation Science Shanghai University of Traditional Chinese MedicineShanghaiChina Engineering Research Center of Traditional Chinese Medicine Intelligent Rehabilitation Ministry of EducationShanghaiChina Department of Rehabilitation Medicine Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Department of Traumatology and Orthopedics Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghaiChina Institute of Science and Technology for Brain-Inspired Intelligence Fudan UniversityShanghaiChina Key Laboratory of Computational Neuroscience and Brain-Inspired Intelligence(Fudan University) Ministry of EducationShanghaiChina Human Phenome Institute Fudan UniversityShanghaiChina

Distinct brain remodeling has been found after different nerve reconstruction strategies,including motor representation of the affected ***,differences among reconstruction strategies at the brain network level have not been *** study aimed to explore intranetwork changes related to altered peripheral neural pathways after different nerve reconstruction surgeries,including nerve repair,endto-end nerve transfer,and end-to-side nerve ***–Dawley rats underwent complete left brachial plexus transection and were divided into four equal groups of eight:no nerve repair,grafted nerve repair,phrenic nerve end-to-end transfer,and end-to-side transfer with a graft sutured to the anterior upper ***-state brain functional magnetic resonance imaging was obtained 7 months after *** independent component analysis algorithm was utilized to identify group-level network components of interest and extract resting-state functional connectivity values of each voxel within the *** in intra-network resting-state functional connectivity were compared among the *** muscle reinnervation was assessed by behavioral observation(elbow flexion)and *** results showed that alterations in the sensorimotor and interoception networks were mostly related to changes in the peripheral neural *** repair was related to enhanced connectivity within the sensorimotor network,while end-to-side nerve transfer might be more beneficial for restoring control over the affected limb by the original motor *** thalamic-cortical pathway was enhanced within the interoception network after nerve repair and end-to-end nerve *** areas related to cognition and emotion were enhanced after end-to-side nerve *** study revealed important brain networks related to different nerve *** networks may be potential targets for enhancing motor recovery.

关键词： brain functional networks end-to-end nerve transfer end-to-side nerve transfer independent component analysis nerve repair peripheral plexus injury resting-state functional connectivity

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Mutual regulation of microglia and astrocytes after Gas6 inhibits spinal cord injury

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Neural Regeneration Research 2025年第2期20卷 557-573页

作者： Jiewen Chen Xiaolin Zeng Le Wang Wenwu Zhang Gang Li Xing Cheng Peiqiang Su Yong Wan xiang Li Department of Spine Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Guangdong Province Key Laboratory of Orthopedics and Traumatology GuangzhouGuangdong ProvinceChina

Invasive inflammation and excessive scar formation are the main reasons for the difficulty in repairing nervous tissue after spinal cord *** and astrocytes play key roles in the spinal cord injury micro-environment and share a close ***,the mechanisms involved remain *** this study,we found that after spinal cord injury,resting microglia(M0)were polarized into pro-inflammatory phenotypes(MG1 and MG3),while resting astrocytes were polarized into reactive and scar-forming *** expression of growth arrest-specific 6(Gas6)and its receptor Axl were significantly down-regulated in microglia and astrocytes after spinal cord *** vitro experiments showed that Gas6 had negative effects on the polarization of reactive astrocytes and pro-inflammatory microglia,and even inhibited the cross-regulation between *** further demonstrated that Gas6 can inhibit the polarization of reactive astrocytes by suppressing the activation of the Yes-associated protein signaling ***,in turn,inhibited the polarization of pro-inflammatory microglia by suppressing the activation of the nuclear factor-κB/p65 and Janus kinase/signal transducer and activator of transcription signaling *** vivo experiments showed that Gas6 inhibited the polarization of pro-inflammatory microglia and reactive astrocytes in the injured spinal cord,thereby promoting tissue repair and motor function ***,Gas6 may play a role in the treatment of spinal cord *** can inhibit the inflammatory pathway of microglia and polarization of astrocytes,attenuate the interaction between microglia and astrocytes in the inflammatory microenvironment,and thereby alleviate local inflammation and reduce scar formation in the spinal cord.

关键词： astrocytes Axl cell polarization Gas6 Hippo signal inflammatory micro-environment intercellular interaction microglia single-cell sequencing spinal cord injury

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Preparation and study of novel mid-to-low-temperature phase-change molten salts

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Science China Technological Sciences 2025年

作者： Chenxing REN Yifan WANG Xin HE Chenghang ZHENG xiang GAO State Key Laboratory of Clean Energy Utilization Zhejiang University Institute of Carbon Neutrality Zhejiang University Baima Lake Laboratory Zhejiang University

Molten salts is a promising medium for heat storage and heat transfer due to their advantages of low cost, low steam pressure, high energy storage density and wide temperature range. In this paper, a new phase change mixture of molten salts with KNO3, NaNO3, ZnCl2, NaF, Na2CO3, NaCl, and MgO as materials has been prepared through a plenty of experiments. The experimental results show that the melting point of some molten salts can be reduced to 202.9°C, and the decomposition temperature can reach 701.3°C. In addition, its thermophysical properties, including specific heat, viscosity and thermal stability, were measured and analyzed. The average specific heat of some molten salts is as high as 2.53 J/（g K）, and the average thermal conductivity is as high as 0.77 W/（m K）. In the 4-day short-term thermal stability test, the fluctuation of each parameter is minimal, and the overall thermal physical property is relatively stable. Compared with the traditional phase change molten salt, the new hybrid molten salt has a significantly lower melting point, a further improvement in the decomposition temperature, specific heat and other comprehensive thermophysical properties, and a better overall economy, which has important application value in heat transfer and energy storage.

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基于荧光光谱和拉曼光谱技术的中药检测研究

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光谱学与光谱分析 2025年第1期45卷 139-145页

作者：逯美红张凡暴雅婷王志军雷海英王翔宇高鹏慧长治学院物理系山西长治046011 西安信息职业大学物理实验中心陕西西安710125 长治学院生命科学系山西长治046011

中医药是中华文化的瑰宝和中华文明的结晶,在全民健康中发挥着重要作用。由于中药生长环境受限及市场需求量的增大,市场上出现了许多以次充好、以假乱真的中药,这对中药发展及质量保障都极为不利。因此,对中药的检测和鉴定非常重要。为... 详细信息

中医药是中华文化的瑰宝和中华文明的结晶,在全民健康中发挥着重要作用。由于中药生长环境受限及市场需求量的增大,市场上出现了许多以次充好、以假乱真的中药,这对中药发展及质量保障都极为不利。因此,对中药的检测和鉴定非常重要。为了探究中药的荧光光谱和拉曼光谱特性,选取白芷、苏木、山药、地榆四种中药进行分类检测研究。一方面,从化学成分和结构的角度分析中药白芷和苏木产生荧光的可能性,采用F-4600型荧光光谱仪(200~750 nm)分别在不同激发波长和不同浓度下测试了白芷和苏木水浸液的荧光光谱,探究了白芷和苏木水浸液的荧光光谱特性,并对荧光强度与不同浓度、激发波长的关系进行了探讨。另一方面,采用共聚焦显微拉曼光谱仪(100~4000 cm^(-1))对4种不同产地的山药和2种不同产地的地榆中药饮片的拉曼光谱分别进行测试研究。结果表明,白芷水浸液在波长260~350 nm的光激发下,产生较强荧光,最佳激发波长是340 nm,荧光峰值波长为420 nm,其荧光强度随浓度的增大而增强,且样品浓度较低时,荧光强度与浓度成线性关系;苏木水浸液在200~290 nm的光激发下,产生较强荧光,最佳激发波长为220 nm,荧光峰值波长为345 nm,随浓度的增加,荧光强度先增强后减弱,在浓度为0.175 mg·mL^(-1)时荧光强度达到最大。白芷水浸液和苏木水浸液的荧光强度都与激发波长的关系呈现高斯分布,符合荧光规律。分析山药和地榆的拉曼光谱,发现不同产地的拉曼特征基本一致,确认山药的拉曼特征峰主要集中在477、862、939、1080、1258、1337和1457 cm^(-1)处;地榆的拉曼特征峰主要集中在862和1337 cm^(-1)处,主要归属与已有的化学成分研究结果相符。部分特征峰处拉曼活性出现差异,可以作为区分不同产地中药的依据。该研究结果不仅为荧光光谱在中药鉴定及质量分析中的应用提供了实验数据和方法参考,也为利用拉曼光谱技术实现中药的快速准确检测及产地归属奠定了基础。

关键词：白芷苏木山药地榆荧光光谱拉曼光谱

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Pro-resolving lipid mediator reduces amyloid-β42–induced gene expression in human monocyte–derived microglia

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Neural Regeneration Research 2025年第3期20卷 873-886页

作者： Ying Wang xiang Zhang Henrik Biverstål Nicolas GBazan Shuai Tan Nailin Li Makiko Ohshima Marianne Schultzberg Xiaofei Li Department of Neurobiology Care Sciences&SocietyDivision of NeurogeriatricsKarolinska InstitutetStockholmSweden Department of Neurology Neuroscience CenterThe First Hospital of Jilin UniversityChangchunJilin ProvinceChina Department of Physiology and Pharmacology Karolinska InstitutetStockholmSweden Department of Biosciences and Nutrition Karolinska InstitutetHuddingeSweden Neuroscience Center of Excellence Louisiana State UniversityNew OrleansLAUSA Department of Medicine SolnaClinical Pharmacology GroupKarolinska University HospitalStockholmSweden

Specialized pro-resolving lipid mediators including maresin 1 mediate resolution but the levels of these are reduced in Alzheimer's disease brain, suggesting that they constitute a novel target for the treatment of Alzheimer's disease to prevent/stop inflammation and combat disease pathology. Therefore, it is important to clarify whether they counteract the expression of genes and proteins induced by amyloid-β. With this objective, we analyzed the relevance of human monocyte–derived microglia for in vitro modeling of neuroinflammation and its resolution in the context of Alzheimer's disease and investigated the pro-resolving bioactivity of maresin 1 on amyloid-β42–induced Alzheimer's disease–like inflammation. Analysis of RNA-sequencing data and secreted proteins in supernatants from the monocyte-derived microglia showed that the monocyte-derived microglia resembled Alzheimer's disease–like neuroinflammation in human brain microglia after incubation with amyloid-β42. Maresin 1 restored homeostasis by down-regulating inflammatory pathway related gene expression induced by amyloid-β42 in monocyte-derived microglia, protection of maresin 1 against the effects of amyloid-β42 is mediated by a re-balancing of inflammatory transcriptional networks in which modulation of gene transcription in the nuclear factor-kappa B pathway plays a major part. We pinpointed molecular targets that are associated with both neuroinflammation in Alzheimer's disease and therapeutic targets by maresin 1. In conclusion, monocyte-derived microglia represent a relevant in vitro microglial model for studies on Alzheimer's disease-like inflammation and drug response for individual patients. Maresin 1 ameliorates amyloid-β42–induced changes in several genes of importance in Alzheimer's disease, highlighting its potential as a therapeutic target for Alzheimer's disease.

关键词： Alzheimer's disease amyloid-β maresin microglia monocyte neuroinflammation resolution RNA-sequencing specialized pro-resolving lipid mediator

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PVT系统解的正则性

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应用数学 2025年第1期38卷 256-262页

作者：向鑫懿张森祥罗宏四川师范大学数学科学学院四川成都610066

物质的气、液、固三态转换是常见的自然现象,常用Physical Vapor Transport(PVT)系统进行描述.本文主要研究PVT系统动力学方程组初边值问题解的存在性及正则性.首先,引入具有变分结构的算子,证明算子的弱连续性,并结合泛函估计证明PVT... 详细信息

物质的气、液、固三态转换是常见的自然现象,常用Physical Vapor Transport(PVT)系统进行描述.本文主要研究PVT系统动力学方程组初边值问题解的存在性及正则性.首先,引入具有变分结构的算子,证明算子的弱连续性,并结合泛函估计证明PVT系统解的存在性;其次,利用能量估计和Gronwall不等式证明PVT系统解的唯一性;最后,通过ADN理论提高了PVT系统解的正则性.

关键词： PVT系统存在性唯一性正则性

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研究性教学模式在光信息专业实验教学中的应用

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实验室研究与探索 2008年第11期27卷 103-105页

作者：雷宏香蔡志岗王嘉辉王福娟中山大学物理科学与工程技术学院广东广州510275

用几个典型示例,介绍研究性教学模式在光信息专业实验教学中的重要应用价值,即将理论模拟作为一种重要研究手段,与专业实验操作密切结合,将实验做深做细,从而提高了学生的学习兴趣和研究性学习能力,获得了较好的教学效果。

关键词：研究性教学模式教学改革专业实验理论模拟

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Current development in quantitative phase-field modeling of solidification

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Journal of Iron and Steel Research International 2017年第9期24卷 865-878页

作者： xiang-lei Dong Hui Xing Kang-rong Weng hong-liang Zhao College of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450001HenanChina Shaanxi Key Laboratory for Condensed Matter Structure and Properties Northwestern Polytechnical UniversityXi'an 710129ShaanxiChina State Key Laboratory of Solidification Processing Northwestern Polytechnical UniversityXi'an 710072ShaanxiChina

The quantitative phase-field simulations were reviewed on the processes of solidification of pure metals and *** quantitative phase-field equations were treated in a diffuse thin-interface limit,which enabled the quantitative links between interface dynamics and model parameters in the quasi-equilibrium *** a result,the quantitative modeling is more effective in dealing with microstructural pattern formation in the large scale simulations without any spurious kinetic *** development of the quantitative phase-field models in modeling the formation of microstructures such as dendritic structures,eutectic lamellas,seaweed morphologies,and grain boundaries in different solidified conditions was also reviewed with the purpose of guiding to find the new prospect of applications in the quantitative phase-field simulations.

关键词： Phase-field modeling Liquid-solid interface Solidification Dendritic growth Microstructural formation

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